scholarly journals Determination of total protein content in biomedical products by the PDMS-assisted lab-in-a-syringe assay using 3D printed scaffolds removal

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Ester Drastíková ◽  
Klára Konderlová ◽  
Andrea Šebestová ◽  
Daniel Baron ◽  
Petra Švecová ◽  
...  
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Low Cost ◽  
Total Protein Content ◽  
Fabrication Procedure ◽  
One Step ◽  
3D Printed ◽  
Bradford Assay ◽  
The One

AbstractThe aim of our work was to develop a low-cost, portable device for the fast and easy determination of total protein content by using PDMS-based lab-in-a-syringe technology with removal of 3D-printed channels. We proposed two designs with a one-step PDMS curing and a two-step PDMS-curing fabrication procedure. The one-step PDMS microdevices were found to be the best in the view of preparation, repeatability, and stability of the reagent. This design was then applied for the determination of total protein content in biomedical products using the Bradford assay.

Retardation Signal for Fluorescent Determination of Total Protein Content via Rapid and Sensitive Chip Moving Reaction Boundary Electrophoretic Titration

2014 ◽  
Vol 86 (6) ◽  
pp. 2888-2894 ◽  
Author(s):  
Houyu Wang ◽  
Yongting Shi ◽  
Jian Yan ◽  
Jingyu Dong ◽  
Si Li ◽  
...  
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Total Protein Content

scholarly journals Analysis of Fatty acid and Determination of Total Protein, Alkaloid, Saponin, Flavonoid of Bangladeshi Bombax ceiba Linn Leaves and Seeds

2016 ◽  
Vol 4 (04) ◽  
pp. 23-28
Author(s):  
Shahin Aziz ◽  
Shahal Ahmed ◽  
Sharmin Akter Lisa ◽  
Tanzima Parvin
Keyword(s):  
Fatty Acid ◽  
Protein Content ◽  
Total Protein ◽  
Petroleum Ether Extract ◽  
Folk Medicine ◽  
Fatty Acid Analysis ◽  
Total Protein Content ◽  
Saponin Content ◽  
Kjeldahl Method

Bombax ceiba Linn belongs to the family of Bombacaceae and is an important medicinal plant. In Bangladesh, Bombax ceiba Linn is locally known as “Shimul tree”. The whole part of the plant used as traditional folk medicine for the treatment of antidysentric, anti diahorreal and antipyretic effects. The present communication attempts to evaluate fatty acid analysis by GC-MS spectrophotometer, total protein content by Kjeldahl method and to quantify some active constituents i.e. alkaloid, saponin and flavonoid. The fatty acid compositions of the petroleum ether extract of leaves and seeds of Bombax ceiba grown in Bangaladesh were determined by gas chromatography- mass spectrophotometer. 8 compounds were identified from leaves and 13 compounds were identified from the seeds. For both cases Palmitic acid showed higher value. The findings from present study showed the protein content for seeds have higher value (18.89%) than leaves of Bombax ceiba . The present investigation showed that both leaves and seeds of Bombax ceiba contain phytochemicals such as flavanoids, alakaloids and saponins in appreciable quantities. The flavonoid content of leaves was 5.97% and for the case of seeds (5.72%), the alkaloid content for leaves was (9.73%) and for seeds (31.44), the saponin content for the case of leaves (13.90%) and for the case of seeds was(43.58%).

Determination of total protein content in soya-bean milk via visual moving reaction boundary titration

2013 ◽  
Vol 31 (11) ◽  
pp. 1064 ◽  
Author(s):  
Chengye GUO ◽  
Houyu WANG ◽  
Lei ZHANG ◽  
Liuyin FAN ◽  
Chengxi CAO
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Soya Bean ◽  
Total Protein Content

scholarly journals Colorimetric determination of total protein content in serum based on the polydopamine/protein adsorption competition on microplates

Talanta ◽  
2019 ◽  
Vol 198 ◽  
pp. 15-22 ◽  
Author(s):  
Pasquale Palladino ◽  
Alvaro Brittoli ◽  
Emanuela Pascale ◽  
Maria Minunni ◽  
Simona Scarano
Keyword(s):  
Protein Content ◽  
Protein Adsorption ◽  
Total Protein ◽  
Total Protein Content ◽  
Colorimetric Determination

scholarly journals Examinations of certain chemical characteristics of fermented dry sausage quality parameters

2012 ◽  
Vol 66 (1-2) ◽  
pp. 73-84 ◽  
Author(s):  
Marija Vukasinovic ◽  
Vladimir Kurcubic ◽  
Vesna Kaljevic ◽  
Pavle Maskovic ◽  
Milun Petrovic
Keyword(s):  
Protein Content ◽  
Meat Products ◽  
Quality Parameters ◽  
Total Protein Content ◽  
Chemical Quality ◽  
Official Gazette ◽  
The One ◽  
Dry Sausages ◽  
High Degree

On the grounds of data available from literature, we observed a high degree of variations in the values of numerous physical-chemical quality parameters of fermented sausages, so that we set as the objective of our investigations to carry out comprehensive examinations of selected chemical quality parameters (total protein content, relative protein content of connective tissue, and humidity in ten different products that belong to the group of fermented dry sausages). The other objective was to compare the values obtained in our investigations with the reference values envisaged under the Regulations on quality and other requirements for meat products (SCG Official Gazette, No. 33/2004) and to determine the percent of products that are found incompatible and the reasons for that. We used two standard reference chemical methods ? the determination of nitrogen and moisture contents (SRPS ISO 937:1992 and SRPS ISO 1442:1998), as well as a modification of method M050 for the determination of hydroxyproline content. We can conclude on the grounds of the results of our investigations that out of the representative number of examined samples (n = 156), originating from 10 different products from the group of fermented dry sausages, 51 samples (32.7%) did not meet the requirements prescribed under the Regulations on quality and other requirements for meat products. Among the rejected samples, the reason for the incompatibility of 40 samples (25.6%) was a a higher level of RPCCT, and 18 samples (11.5%) had a higher moisture content than permitted. None of the total number of examined samples had a content of total proteins lower than the one prescribed under Regulations.

Seromucoid Determination: Variables and Modifications

1960 ◽  
Vol 6 (3) ◽  
pp. 189-198 ◽  
Author(s):  
T M Asher ◽  
G R Cooper
Keyword(s):  
Environmental Factors ◽  
Protein Content ◽  
Perchloric Acid ◽  
Total Protein ◽  
Standard Procedure ◽  
Total Protein Content ◽  
Inflammatory Condition ◽  
Freezing And Thawing ◽  
Significant Difference

Abstract The technic of Winzler (1) for the determination of seromucoid has been studied to measure its reproducibility and the effect of certain environmental factors. Values obtained are fairly reproducible (a difference of 45 mg./l00 ml. BSAP between two specimens indicates a significant difference at the P = 0.05 level). Variables such as temperature, freezing and thawing, speed of addition of perchloric acid, and total protein content of serum have no practical influence on the final SM values. A modification in technic involving determinations on three different dilutions of a specimen gives differences greater than those found with the standard procedure for sera from patients with an inflammatory condition.

scholarly journals Blue lupine seeds protein content and amino acids composition

2018 ◽  
Vol 64 (No. 4) ◽  
pp. 147-155 ◽  
Author(s):  
Tomczak Aneta ◽  
Zielińska-Dawidziak Magdalena ◽  
Piasecka-Kwiatkowska Dorota ◽  
Lart-Szczapa Eleonora
Keyword(s):  
Amino Acids ◽  
Protein Content ◽  
Total Protein ◽  
Ultra Performance Liquid Chromatography ◽  
Point Of View ◽  
Total Protein Content ◽  
Amino Acids Composition ◽  
Kjeldahl Method ◽  
The One ◽  
Two Parameters

Lupine seeds are promising soy replacement in food products and feeds. Eighteen cultivars of blue lupine seeds were examined to indicate the one most favourable in protein content and quality. Two parameters were studied, i.e. total protein content (with the Kjeldahl method) and amino acids composition (with the ultra performance liquid chromatography technique). Both parameters studied were variable and depended both on the cultivar and on the place of cultivation. Protein content was in the range of 28–41% and the worst cultivar, regardless of cultivation place, was cv. Kalif (average total protein content 29.37 ± 1.14%), while the best cultivar was Boruta (average total protein content 37.43 ± 0.98%). The blue lupine seeds were rich especially in leucine (5.3 ± 0.5–9.7 ± 0.5 g/16 g N), threonine (2.4 ± 0.7–4.9 ± 0.1 g/16 g N) and lysine (2.7 ± 0.4–5.6 ± 0.1 g/16 g N). The richest among all amino acids studied were the cv. Bojar seeds from Wiatrowo and cv. Oskar from Przebędowo. It was not possible to choose one cultivar preferable from the nutritional point of view, because a strong influence of cultivation place on protein content and quality was observed, especially as to the precipitation sum.

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