scholarly journals USE OF MALE STERILITY AND SYNTHESIS OF MAINTAINER LINE FOR HYBRID SEED PRODUCTION IN ONION (Allium cepa L.)

2016 ◽  
Vol 29 (1) ◽  
pp. 31-38
Author(s):  
G. M. Mohsin ◽  
Farruk Ahmed ◽  
M. S. Rahman ◽  
M. S. Islam
Keyword(s):  
Male Sterility ◽  
F1 Hybrids ◽  
Recurrent Parent ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Maintainer Line ◽  
Local Cultivar ◽  
Allium Cepa L ◽  
Hybrid Variety

Field experiment was carried out and one cytoplasmic genetic male sterile line (Smsms) and two local lines were identified as maintainer lines (Fmsms). These two crossed materials namely 3(Shallot*Local) and 4 (Shallot *Local) produced 100 per cent male sterile progeny in full sib and backcross generations. The Shallot * market cultivar produced both male fertile and male sterile segregating progenies. It indicated that the market material is probably determined by dominant and recessive independently acting genes which genetically impure lines. All other crossed materials produced 100 per cent male fertile progeny upon crossing with shallot. So, in backcross generations, the male sterile plants were more when local was used as recurrent parent. Thus, the local cultivar can be used as maintainer line for “Shallot”. The performance of 904 F1 and 905 F1 hybrids over check and better parent was found to be preferably better using CMS system. So, considering the genetics and stability of the male sterility mechanism, further studies are needed towards hybrid variety development in Bangladesh.

Interspecific introgression of male sterility from tetraploid oilseed Brassica napus to diploid vegetable B. rapa through hybridisation and backcrossing

2013 ◽  
Vol 64 (7) ◽  
pp. 652 ◽  
Author(s):  
Zhengjie Wan ◽  
Yuanbao Tan ◽  
Minhui Shi ◽  
Yuejin Xu ◽  
Nader Aryamanesh ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Male Sterility ◽  
Morphological Characteristics ◽  
Female Parent ◽  
F1 Hybrids ◽  
Recurrent Parent ◽  
Male Sterile ◽  
Interspecific Introgression ◽  
C Genome ◽  
Inbreeding Line

Interspecific F1 hybrids were obtained from a cross between a male sterile Brassica napus (2n = 4x = 38, AA (20) and CC (18) genomes) and an inbreeding line B. rapa (Purple Cai-Tai inbred line 9418, 2n = 2x = 20, AA (20) genome) to introgress male sterility from a tetraploid into a diploid through backcrossing. The morphological characteristics of F1 plants were more like the female parent B. napus and segregated considerably in BC1 when backcrossed to the recurrent parent Purple Cai-Tai. The progeny became stable and more similar to Purple Cai-Tai by BC4. Most C genome chromosomes were found to be eliminated, based on cytogenetic analysis. The majority of chromosomes were eliminated at very early backcross stages, with only 20–26 chromosomes in BC1 plants, and some chromosomes were eliminated gradually with increased backcross generations. The BC4 plants were generally stable with exactly 20 chromosomes. Analysis by AFLP indicated that 49.5–68.7% of the total bands eliminated from F1 to BC4 were female parent specific, and ~12% of B. napus bands were retained with increased backcrossing. The genetic materials controlling sterility from the female parent B. napus were introgressed successfully into the BC4 plants even though most B. napus chromosomes/genetic materials were eliminated during the backcross process.

Male sterility in field beans (Vicia faba L.). II. Yield trials of F1 hybrid winter beans produced with the aid of male sterility

1964 ◽  
Vol 63 (2) ◽  
pp. 235-243 ◽  
Author(s):  
D. A. Bond ◽  
J. L. Fyfe ◽  
Gillian Toynbee-Clarke
Keyword(s):  
Male Sterility ◽  
Inbred Lines ◽  
F1 Hybrids ◽  
Male Sterile ◽  
Male Sterile Line ◽  
F1 Hybrid ◽  
Vicia Faba L ◽  
The Mean ◽  
Yield Trials ◽  
Field Beans

1. F1 hybrids between inbred lines of winter beans were obtained by roguing male-fertile plants from segregating progenies of a genetic male-sterile line which had been interplanted with a pollinator.2. In a series of trials the mean yields of many hybrids were significantly higher than those of open-pollinated varieties. Over the 4 years, 1959–62, with trials at three centres in each year, the mean advantages of all hybrids over the two control varieties was 6·4 cwt./acre over Gartons S.Q., 4·7 cwt./acre over Throws M.S.

scholarly journals Early Maturing Male Sterile Line ‘Wonye 30005’ for Hybrid Seed Production of Onion (Allium cepa L.)

2016 ◽  
Vol 48 (2) ◽  
pp. 168-172
Author(s):  
Cheol-Woo Kim ◽  
Eul-Tai Lee ◽  
In-Hu Choi ◽  
Young-Seok Jang ◽  
Sang-kyeong Bae ◽  
...  
Keyword(s):  
Seed Production ◽  
Allium Cepa ◽  
Hybrid Seed ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Allium Cepa L ◽  
Early Maturing

scholarly journals Search for cytoplasmic male sterility plants and its maintainer plants of carrots in Rogneda variety

2020 ◽  
pp. 42-44
Author(s):  
A. V. Kornev ◽  
A. N. Khovrin ◽  
V. I. Leunov ◽  
S. N. Derevschukov ◽  
L. V. Sycheva
Keyword(s):  
Male Sterility ◽  
Federal State ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Maintainer Line ◽  
Maternal Line ◽  
Paternal Line ◽  
Russian Research Institute ◽  
Experimental Base ◽  
Population Breeding

Relevance. Hybrid seed production of carrots is carried out on a three-line basis, which suggests the presence of a male sterile maternal line and a fertile paternal line. For the reproduction of a male sterile line, a maintainer line is needed. The process of creating an isogenic pair of a manly sterile line – a maintainer line is of great complexity. As a maintainer line, it is necessary to use only a plant – a homozygote Cyt N msms for the core genes providing sterility, but with a normal cytoplasm. The purpose of the work is the creation of maintainer line for the sterility of carrots from the population of the Rogneda variety.Methods. Research were carried out: in 2016, at the experimental base of the Voronezh Vegetable Experimental Station (Voronezh Region); in 2017 - 2019, at the breeding center of All-Russian Research Institute of Vegetable Growing – branch of Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (Moscow Region). The material for the work was seed plants and roots of the Rogneda variety and hybrid, inbred, backcross progenies obtained from this population. Breeding methods: hybridization, inbreeding. Crosses used: simple direct and complex return (backcrosses), inbreeding.Results.Research revealed the possibility of searching for a maintainer line in the Rogneda variety. The P43f inbred progeny is potential line B, which maintains the CMS trait of the petaloid type at 100%. Of the 67 hybrid progenies, only P43st x P43f was left, in which all plants with male sterility. 65 hybrid progenies contained both sterile and fertile plants. Associated 65 inbred progenies had sterile and fertile plants, i.e. it makes no sense to try to obtain a maintainer line is needed. The process of creating an isogenic pair of a manly sterile line – a maintainer line from such fertile plants. Hybrid progeny of P4st x P4f had 100% fertile plants. The inbred progeny of P4f was represented only by fertile plants. 

scholarly journals Identifikasi Galur-galur Elit Padi (Oryza sativa) untuk Tetua Padi Hibrida

2017 ◽  
Vol 10 (1) ◽  
pp. 12
Author(s):  
Yudhistira Nugraha ◽  
E. Lubis ◽  
M. Diredja
Keyword(s):  
Hybrid Rice ◽  
Rice Yield ◽  
Parental Line ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Maintainer Line ◽  
Pests And Diseases ◽  
Four Seasons ◽  
Hybrid Variety ◽  
Parental Lines

<p><strong>Abstract</strong></p><p>One of the efforts to increase rice yield is through hybrid rice production. Hybrid rice has now been commercialized in Indonesia. The parental lines of the hybrids developed in Indonesia mostly came from introduced lines. Those lines were more susceptible to stresses, especially to pests and diseases, that make the yield of the hybrid fluctuated and wasn&amp;rsquo;t stable. Hence, it is needed to develop hybrid variety using Indonesian parental line which were well adapted to Indonesia. Therefore, identification of those lines is needed. In the last four seasons 2000/01 WS until 2002 DS, 1471 lines have been test-crossed in Muara experimental station, Bogor. Out of 2769 hybrids developed from 1471 parental lines, 18.1%, 74.5%, and 7.1% were fertile, partial fertile or partial sterile and sterile, respectivively. The frequency of fertile plants tested in the observation yield trail was 3.4% and only 0.7% of sterile plants were continued in the backcross program for new cytoplasmic male-sterile line development. Several number of lines identified as restorer lines were progenies of popular variety, namely IR64 and IR36. While those six lines which identified as maintainer line came from crosser of Ptb 33 and IR3043.</p><p><strong>Abstrak</strong></p><p>Salah satu upaya untuk meningkatan hasil padi adalah dengan penanaman varietas hibrida. Saat ini di Indonesia mulai menerapkan teknologi padi hibrida secara komersial. Padi hibrida yang berkembang di Indonesia berasal dari galur-galur tetua hasil introduksi. Biasanya galur-galur tetua introduksi lebih peka terhadap perubahan kondisi lingkungan terutama hama dan penyakit, sehingga hasilnya lebih berfluktuasi dan tidak stabil. Dengan demikian perlu dirakit varietas padi hibrida dengan menggunakan galur padi hasil pemuliaan Balai Penelitian Tanaman Padi (Balitpa) yang telah beradaptasi baik di Indonesia. Galur-galur tersebut perlu diidentifikasi sebelum digunakan sebagai tetua padi hibrida dengan cara diuji silang. Selama empat musim, yaitu MH 2000/2001sampai MK 2002, sebanyak 1471 galur diuji silang di Inlitpa Muara, Bogor. Sebanyak 2769 hibrida hasil uji silang dengan 1471 galur tetua teridentifikasi 18,1%, 74,5%, dan 7,1% berturut-turut fertil, parsial fertil atau steril, dan steril. Sebanyak 3,4% tanaman fertil dilanjutkan ke pertanaman observasi daya hasil dan hanya 0,7% tanaman steril yang dilanjutkan ke program silang balik untuk pembentukan galur mandul jantan baru. Sebanyak 16 galur yang teridentifikasi sebagai galur pemulih kesuburan berasal dari keturunan varietas populer IR64 dan IR36. Sementara itu enam galur yang teridentifikasi sebagai galur pelestari mandul jantan berasal dari persilangan Ptb 33 dan IR3043.</p>

scholarly journals Combined Transcriptome and Proteome Analysis of Anthers of AL-type Cytoplasmic Male Sterile Line and Its Maintainer Line Reveals New Insights into Mechanism of Male Sterility in Common Wheat

2021 ◽  
Vol 12 ◽  
Author(s):  
Miaomiao Hao ◽  
Wenlong Yang ◽  
Tingdong Li ◽  
Muhammad Shoaib ◽  
Jiazhu Sun ◽  
...  
Keyword(s):  
Male Sterility ◽  
Proteome Analysis ◽  
Acid Synthesis ◽  
Anther Development ◽  
Ros Scavenging ◽  
Male Sterile ◽  
Pollen Abortion ◽  
Male Sterile Line ◽  
Maintainer Line ◽  
New Perspective

Cytoplasmic male sterility (CMS) plays an essential role in hybrid seeds production. In wheat, orf279 was reported as a CMS gene of AL-type male sterile line (AL18A), but its sterility mechanism is still unclear. Therefore, transcriptomic and proteomic analyses of the anthers of AL18A and its maintainer line (AL18B) were performed to interpret the sterility mechanism. Results showed that the electron transport chain and ROS scavenging enzyme expression levels changed in the early stages of the anther development. Biological processes, i.e., fatty acid synthesis, lipid transport, and polysaccharide metabolism, were abnormal, resulting in pollen abortion in AL18A. In addition, we identified several critical regulatory genes related to anther development through combined analysis of transcriptome and proteome. Most of the genes were enzymes or transcription factors, and 63 were partially homologous to the reported genic male sterile (GMS) genes. This study provides a new perspective of the sterility mechanism of AL18A and lays a foundation to study the functional genes of anther development.

Inheritance of thermo-photoperiod sensitive male sterility in wheat

2006 ◽  
Vol 57 (2) ◽  
pp. 187 ◽  
Author(s):  
Rui-Xing Guo ◽  
Dong-Fa Sun ◽  
Xun-Dong Cheng ◽  
De-Fu Rong ◽  
Chengdao Li
Keyword(s):  
High Temperature ◽  
Low Temperature ◽  
Male Sterility ◽  
Common Wheat ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Wheat Varieties ◽  
Sowing Dates ◽  
Recessive Genes

The fertility of a wheat male sterile line 337S was investigated in 4 consecutive years with 18 different sowing dates. Line 337S showed high sterility under both short daylength/low temperature and long daylength/high temperature during ear development. It has 2 time windows to be used as a male sterile line for hybrid seed production. Its fertility rate can be >50% with suitable sowing time; thus, it can be self-maintained as a male sterile line. Line 337S was reciprocally crossed with 7 common wheat varieties and the fertility of their F1, F2, and BC1 hybrids was investigated at different sowing dates. The results showed that recessive nuclear genes controlled male sterility in 337S and no cytoplasmic effect was observed. All common wheat varieties were able to restore its fertility. The male sterility was controlled by a pair of recessive genes under short daylength/low temperature, but was governed by 2 pairs of recessive genes under long daylength/high temperature. This novel male sterile line provides a new tool for using heterosis in wheat.

scholarly journals INHERITANCE OF FERTILITY RESTORATION INVOLVING WILD ABORTIVE CYTOPLASMIC MALE STERILITY SYSTEM IN RICE (Oryza sativa L.)

2011 ◽  
Vol 24 (1) ◽  
pp. 33-40
Author(s):  
M. J. Hasan ◽  
M. U. Kulsum ◽  
A. Ansari ◽  
A. K. Paul ◽  
P. L. Biswas
Keyword(s):  
Oryza Sativa ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Gene Action ◽  
Oryza Sativa L ◽  
Fertility Restoration ◽  
Dominant Gene ◽  
Recessive Gene ◽  
Male Sterile ◽  
Male Sterile Line

Inheritance of fertility restoration was studied in crosses involving ten elite restorer lines of rice viz. BR6839-41-5-1R, BR7013-62-1-1R, BR7011-37-1-2R, BR10R, BR11R, BR12R, BR13R, BR14R, BR15R and BR16R and one male sterile line Jin23A with WA sources of cytoplasmic male sterility. The segregation pattern for pollen fertility of F2 and BC1 populations of crosses involving Jin23A indicated the presence of two independent dominant fertility restoring genes. The mode of action of the two genes varied in different crosses revealing three types of interaction, i.e. epistasis with dominant gene action, epistasis with recessive gene action, and epistasis with incomplete dominance.DOI: http://dx.doi.org/10.3329/bjpbg.v24i1.16997

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