scholarly journals Search for cytoplasmic male sterility plants and its maintainer plants of carrots in Rogneda variety

2020 ◽  
pp. 42-44
Author(s):  
A. V. Kornev ◽  
A. N. Khovrin ◽  
V. I. Leunov ◽  
S. N. Derevschukov ◽  
L. V. Sycheva
Keyword(s):  
Male Sterility ◽  
Federal State ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Maintainer Line ◽  
Maternal Line ◽  
Paternal Line ◽  
Russian Research Institute ◽  
Experimental Base ◽  
Population Breeding

Relevance. Hybrid seed production of carrots is carried out on a three-line basis, which suggests the presence of a male sterile maternal line and a fertile paternal line. For the reproduction of a male sterile line, a maintainer line is needed. The process of creating an isogenic pair of a manly sterile line – a maintainer line is of great complexity. As a maintainer line, it is necessary to use only a plant – a homozygote Cyt N msms for the core genes providing sterility, but with a normal cytoplasm. The purpose of the work is the creation of maintainer line for the sterility of carrots from the population of the Rogneda variety.Methods. Research were carried out: in 2016, at the experimental base of the Voronezh Vegetable Experimental Station (Voronezh Region); in 2017 - 2019, at the breeding center of All-Russian Research Institute of Vegetable Growing – branch of Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (Moscow Region). The material for the work was seed plants and roots of the Rogneda variety and hybrid, inbred, backcross progenies obtained from this population. Breeding methods: hybridization, inbreeding. Crosses used: simple direct and complex return (backcrosses), inbreeding.Results.Research revealed the possibility of searching for a maintainer line in the Rogneda variety. The P43f inbred progeny is potential line B, which maintains the CMS trait of the petaloid type at 100%. Of the 67 hybrid progenies, only P43st x P43f was left, in which all plants with male sterility. 65 hybrid progenies contained both sterile and fertile plants. Associated 65 inbred progenies had sterile and fertile plants, i.e. it makes no sense to try to obtain a maintainer line is needed. The process of creating an isogenic pair of a manly sterile line – a maintainer line from such fertile plants. Hybrid progeny of P4st x P4f had 100% fertile plants. The inbred progeny of P4f was represented only by fertile plants. 

scholarly journals USE OF MALE STERILITY AND SYNTHESIS OF MAINTAINER LINE FOR HYBRID SEED PRODUCTION IN ONION (Allium cepa L.)

2016 ◽  
Vol 29 (1) ◽  
pp. 31-38
Author(s):  
G. M. Mohsin ◽  
Farruk Ahmed ◽  
M. S. Rahman ◽  
M. S. Islam
Keyword(s):  
Male Sterility ◽  
F1 Hybrids ◽  
Recurrent Parent ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Maintainer Line ◽  
Local Cultivar ◽  
Allium Cepa L ◽  
Hybrid Variety

Field experiment was carried out and one cytoplasmic genetic male sterile line (Smsms) and two local lines were identified as maintainer lines (Fmsms). These two crossed materials namely 3(Shallot*Local) and 4 (Shallot *Local) produced 100 per cent male sterile progeny in full sib and backcross generations. The Shallot * market cultivar produced both male fertile and male sterile segregating progenies. It indicated that the market material is probably determined by dominant and recessive independently acting genes which genetically impure lines. All other crossed materials produced 100 per cent male fertile progeny upon crossing with shallot. So, in backcross generations, the male sterile plants were more when local was used as recurrent parent. Thus, the local cultivar can be used as maintainer line for “Shallot”. The performance of 904 F1 and 905 F1 hybrids over check and better parent was found to be preferably better using CMS system. So, considering the genetics and stability of the male sterility mechanism, further studies are needed towards hybrid variety development in Bangladesh.

scholarly journals Genetic Analysis and Fine Mapping of a Spontaneously Mutated Male Sterility Gene in Brassica rapa ssp. chinensis

2020 ◽  
Vol 10 (4) ◽  
pp. 1309-1318
Author(s):  
Tzu-Kai Lin ◽  
Ya-Ping Lin ◽  
Shun-Fu Lin
Keyword(s):  
Male Sterility ◽  
Fine Mapping ◽  
Genotyping By Sequencing ◽  
Snp Markers ◽  
Hybrid Seed Production ◽  
Spontaneous Mutant ◽  
Male Sterile ◽  
Sterility Gene ◽  
Intron 4 ◽  
Male Sterility Gene

Male sterility has been widely used in hybrid seed production in Brassica, but not in B. rapa ssp. chinensis, and genetic models of male sterility for this subspecies are unclear. We discovered a spontaneous mutant in B. rapa ssp. chinensis. A series of progeny tests indicated that male sterility in B. rapa ssp. chinensis follows a three-allele model with BrMsa, BrMsb, and BrMsc. The male sterility locus has been mapped to chromosome A07 in BC1 and F2 populations through genotyping by sequencing. Fine mapping in a total of 1,590 F2 plants narrowed the male sterility gene BrMs to a 400 kb region, with two SNP markers only 0.3 cM from the gene. Comparative gene mapping shows that the Ms gene in B. rapa ssp. pekinensis is different from the BrMs gene of B. rapa ssp. chinensis, despite that both genes are located on chromosome A07. Interestingly, the DNA sequence orthologous to a male sterile gene in Brassica napus, BnRf, is within 400 kb of the BrMs locus. The BnRf orthologs of B. rapa ssp. chinensis were sequenced, and one KASP marker (BrMs_indel) was developed for genotyping based on a 14 bp indel at intron 4. Cosegregation of male sterility and BrMs_indel genotypes in the F2 population indicated that BnRf from B. napus and BrMs from B. rapa are likely to be orthologs. The BrMs_indel marker developed in this study will be useful in marker-assisted selection for the male sterility trait.

scholarly journals Potential Association of Reactive Oxygen Species With Male Sterility in Peach

2021 ◽  
Vol 12 ◽  
Author(s):  
Yaming Cai ◽  
Zhishen Ma ◽  
Collins Otieno Ogutu ◽  
Lei Zhao ◽  
Liao Liao ◽  
...  
Keyword(s):  
Male Sterility ◽  
Pollen Grains ◽  
Hybrid Vigor ◽  
Abnormal Development ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Potential Association ◽  
Genetic Mechanisms ◽  
Ros Burst ◽  
Underlying Mechanisms

Male sterility is an important agronomic trait for hybrid vigor utilization and hybrid seed production, but its underlying mechanisms remain to be uncovered. Here, we investigated the mechanisms of male sterility in peach using a combined cytology, physiology, and molecular approach. Cytological features of male sterility include deformed microspores and tapetum cells along with absence of pollen grains. Microspores had smaller nucleus at the mononuclear stage and were compressed into belts and subsequently disappeared in the anther cavity, whereas tapetum cells were swollen and vacuolated, with a delayed degradation to flowering time. Male sterile anthers had an ROS burst and lower levels of major antioxidants, which may cause abnormal development of microspores and tapetum, leading to male sterility in peach. In addition, the male sterility appears to be cytoplasmic in peach, which could be due to sequence variation in the mitochondrial genome. Our results are helpful for further investigation of the genetic mechanisms underlying male sterility in peach.

scholarly journals CHARACTERIZATION OF A MALE-STERILE SYSTEM WITH A CLOSELY LINKED SEEDLING MARKER TO FACILITATE F1 HYBRID TOMATO SEED PRODUCTION

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1168e-1168 ◽  
Author(s):  
Edward C. Tigchelaar
Keyword(s):  
Male Sterility ◽  
Seed Production ◽  
Marker Gene ◽  
Hybrid Seed ◽  
Hybrid Seed Production ◽  
Chromosome 2 ◽  
Male Sterile ◽  
Tomato Seed ◽  
F1 Hybrid

The coupling phase linkages have been synthesized between the gene aw (without anthocyanin) and the male sterile gene ms15 (and its alleles ms26, ms47, and an Israeli source of male sterility). Less than 2 map units separate aw and ms15 on chromosome 2, providing a convenient seedling marker gene to rapidly identify male sterility for both inbred development and hybrid seed production. The seedling marker also provides a convenient marker to rapidly assess hybrid seed purity. Unique features of each of the alleles involved in male sterility and their use in inbred and hybrid development will be described.

Selection of Nuclear Genotypes Associated with the Thermo-inducibility of Owen-type Cytoplasmic Male Sterility in Sugar Beet (Beta vulgaris L.)

2021 ◽  
Author(s):  
Hiroaki Matsuhira ◽  
Kazuyoshi Kitazaki ◽  
Katsunori Matsui ◽  
Keisi Kubota ◽  
Yosuke Kuroda ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Beta Vulgaris ◽  
Parental Line ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Flowering Season ◽  
Restorer Of Fertility ◽  
The Stability

Abstract The stability of cytoplasmic male sterility expression in several genetic backgrounds was investigated in sugar beet (Beta vulgaris L.). Nine genetically heterogenous plants from old cultivars were crossed with a cytoplasmic male-sterile line to obtain 266 F1 plants. Based on marker analysis using a multiallelic DNA marker linked to restorer-of-fertility 1 (Rf1), we divided the F1 plants into 15 genotypes. We evaluated the phenotypes of the F1 plants under two environmental conditions: greenhouse rooms with or without daytime heating during the flowering season. Three phenotypic groups appeared: those consistently expressing male sterility (MS), those consistently having restored pollen fertility, and those expressing MS in a thermo-sensitive manner. All plants in the consistently male-sterile group inherited a specific Rf1 marker type named p4. We tested the potential for thermo-induced male-sterile plants to serve as seed parents for hybrid seed production, and three genotypes were selected. Open pollination by a pollen parental line with a dominant trait of red-pigmented hypocotyls and leaf veins resulted in seed setting on thermo-induced male-sterile plants, indicating that their female organs were functional. More than 99.9% of the progeny expressed the red pigmentation trait; hence, highly pure hybrids were obtained. We determined the nucleotide sequences of Rf1 from the three genotypes: one had a novel allele and two had known alleles, of which one was reported to have been selected previously as a nonrestoring allele at a single US breeding station but not at other stations in the US, or in Europe or Japan, suggesting environmental sensitivity.

Development and evaluation of Diplotaxis muralis (mur) cytoplasmic male sterility system in summer rape

2003 ◽  
Vol 83 (2) ◽  
pp. 261-269 ◽  
Author(s):  
T. C. Riungu ◽  
P. B. E. McVetty
Keyword(s):  
Male Sterility ◽  
Floral Morphology ◽  
Seedling Emergence ◽  
Temperature Stability ◽  
Line Pair ◽  
Male Sterile ◽  
Maintainer Line ◽  
Brassica Napus L ◽  
Pair Comparisons ◽  
Winter Rapeseed

The Diplotaxis muralis male sterility inducing cytoplasm (mur cytoplasm) and its maintainer genes were transferred from the winter rapeseed male sterile line Mangun mur CMS A-line and the mur CMS maintainer line Mangun mur CMS B-line, respectively, into summer rape lines genetically related to the open-pollinated population cultivars Regent and Karat using standard cross, backcross and test cross breeding procedures. Three genetically identical summer rape mur CMS A-line and B-line pairs, Karat I A-line and B-line, Karat II A-line and B-line and Regent A-line and B line were developed. Flower morphology studies revealed that the mur CMS A-lines had anthers displaying a complete absence of pollen and floral morphology modifications similar to that seen for other CMS systems in summer rape. Seedling emergence, days to first flower, days to maturity, and height of the vernalized mur CMS Mangun A-line and B-line pair and the summer rape mur CMS A-line and B-line pairs were similar among treatments and equal for A-line and B-line pair comparisons within cultivars for plants grown in the greenhouse. There was no evidence of biological benefits or costs associated with the mur cytoplasm. The male sterility for the vernalized Mangun mur CMS A-line and the summer rape mur CMS A-lines was stable at temperatures up to 30/24EC. Key words: Brassica napus L., male sterility, temperature stability

scholarly journals Deciphering the dynamic gene expression patterns of pollen abortion in a male sterile line of Avena sativa through transcriptome analysis at different developmental stages

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Lijun Zhang ◽  
Mingchuan Ma ◽  
Lin Cui ◽  
Longlong Liu
Keyword(s):  
Gene Expression ◽  
Male Sterility ◽  
Developmental Stages ◽  
Expression Patterns ◽  
Hybrid Breeding ◽  
Gene Expression Patterns ◽  
Hybrid Seed Production ◽  
Isogenic Line ◽  
Male Sterile ◽  
Pollen Abortion

Abstract Background Male sterility (MS) has important applications in hybrid seed production, and the abortion of anthers has been observed in many plant species. While most studies have focused on the genetic factors affecting male sterility, the dynamic gene expression patterns of pollen abortion in male sterile lines have not been fully elucidated. In addition, there is still no hybrid oat that is commercially planted due to the lack of a suitable system of male sterility for hybrid breeding. Results In this study, we cultivated a male sterile oat line and a near-isogenic line by crossbreeding to elucidate the expression patterns of genes that may be involved in sterility. The first reported CA male sterile (CAMS) oat line was used for cross-testing and hybridization experiments and was confirmed to exhibit a type of nuclear sterility controlled by recessive genes. Oat stamens of two lines were sampled at four different developmental stages separately. Paired-end RNA sequencing was performed for each sample and generated 252.84 Gb sequences. There were 295,462 unigenes annotated in public databases in all samples, and we compared the histological characteristics and transcriptomes of oat stamens from the two oat lines at different developmental stages. Our results demonstrate that the sterility of the male sterile oat line occurs in the early stage of stamen development and is primarily attributable to abnormal meiosis and the excessive accumulation of superoxide. Conclusions To the best of our knowledge, this study is the first to decipher the dynamic expression profiles of pollen abortion CAMS and CA male fertile (CAMF) oat lines, which may represent a valuable resource for further studies attempting to understand pollen abortion and anther development in oats.

scholarly journals Comparative Transcriptome Analysis of the Anthers from the Cytoplasmic Male-Sterile Pepper Line HZ1A and Its Maintainer Line HZ1B

Horticulturae ◽  
2021 ◽  
Vol 7 (12) ◽  
pp. 580
Author(s):  
Zhixing Nie ◽  
Jianying Chen ◽  
Yunpeng Song ◽  
Hongfei Fu ◽  
Hong Wang ◽  
...  
Keyword(s):  
Male Sterility ◽  
Molecular Mechanisms ◽  
Male Sterile ◽  
Maintainer Line ◽  
Breeding Programs ◽  
Kegg Pathways ◽  
Kegg Analysis ◽  
Cms Line ◽  
Phenylpropanoid Biosynthesis ◽  
Go Terms

Cytoplasmic male-sterility (CMS) is important for the utilization of crop heterosis and study of the molecular mechanisms involved in CMS could improve breeding programs. In the present study, anthers of the pepper CMS line HZ1A and its maintainer line HZ1B were collected from stages S1, S2, and S3 for transcriptome sequencing. A total of 47.95 million clean reads were obtained, and the reads were assembled into 31,603 unigenes. We obtained 42 (27 up-regulated and 15 down-regulated), 691 (346 up-regulated and 345 down-regulated), and 709 (281 up-regulated and 428 down-regulated) differentially expressed genes (DEGs) in stages S1, S2, and S3, respectively. Through Gene Ontology (GO) analysis, the DEGs were found to be composed of 46 functional groups. Two GO terms involved in photosynthesis, photosynthesis (GO:0015986) and photosystem I (GO:0009522), may be related to CMS. Through Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, oxidative phosphorylation (ko00190) and phenylpropanoid biosynthesis (ko00940) were significantly enriched in the S1 and S2 stages, respectively. Through the analysis of 104 lipid metabolism-related DEGs, four significantly enriched KEGG pathways may help to regulate male sterility during anther development. The mitochondrial genes orf470 and atp6 were identified as candidate genes of male sterility for the CMS line HZ1A. Overall, the results will provide insights into the molecular mechanisms of pepper CMS.

scholarly journals Morfologi Bunga dan Korelasinya terhadap Kemampuan Menyerbuk Silang Galur Mandul Jantan Padi

2014 ◽  
Vol 33 (2) ◽  
pp. 109
Author(s):  
Indrastuti A. Rumanti ◽  
B. S. Purwoko ◽  
Iswari S. Dewi ◽  
Hajrial Aswidinnoor ◽  
Satoto Satoto
Keyword(s):  
Seed Set ◽  
Block Design ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Maintainer Line ◽  
Field Station ◽  
Cms Line ◽  
Randomized Complete Block Design ◽  
Flowering Behaviour ◽  
Stigma Exsertion

Good male sterile lines should have high and stable sterility, as well as have good characters of flowers that enhances the outcrossing ability. Research was conducted at Sukamandi field station during DS of 2010 to study the character and flowering behaviour and their genetic variability among the new CMS lines. Five new CMS lines were tested in the field in three replicates of randomized complete block design. The results showed that CMS derived from the Wild Abortive, Kalinga and Gambiaca all were early flowering. The new CMS lines have better flower characters than that of IR58025A, including the following: bigger stigma, higher stigma exsertion percentage, wider degree of glume opening, and longer duration of glume opening. Better flowering behaviour enhanced the ability of outcrossing, and resulted in seed set obtained from CMS lines, up to 25.90%; the traditional IR58025A CMS line produced seed set of only 2.98%. The significant positive correlation was found between seed set with stigma width (r = 0.44*), with stigma exsertion (r = 0.54*); and with degree of glume opening of male sterile lines (r = 0.42*); also with filament length (r = 0.47*) and degree of glume opening of maintainer line (r = 0.57**). Those characters are important for the seed set of CMS lines in the hybrid seed production.

scholarly journals Sunflower hybrid Status

2021 ◽  
Vol 187 (3) ◽  
pp. 91-95
Author(s):  
O.F. Gorbachenko ◽  
◽  
T.V. Usatenko ◽  
N.S. Luchkina ◽  
N.A. Zhitnik ◽  
...  
Keyword(s):  
Downy Mildew ◽  
Wide Distribution ◽  
Early Maturity ◽  
Maternal Line ◽  
High Productivity ◽  
Oil Crops ◽  
Paternal Line ◽  
Russian Research Institute ◽  
Average Plant ◽  
Favorable Conditions

The widespread saturation of crop rotations with sunflower crops contributed to the creation of favorable conditions for the emergence and wide distribution of new more aggressive races of broomrape, downy mildew and other pathogens of this crop. Sunflower varieties and hybrids resistant to previously common races have become severely affected. One of the ways to solve this problem is to develop sunflower hybrids resistant to new virulent races. The breeders had to combine high productivity and genes of resistance to new more aggressive races of broomrape and downy mildew in one genotype. The first stage of the work was development of a new breeding germplasm and selection of parental lines with breeding valuable traits from it. Based on these lines, there were developed hybrid combinations resistant to new highly virulent races of broomrape and downy mildew. The hybrid Status was developed by hybridization of a maternal line ED 47 and a paternal line ED 193. The maternal line ED 47 is single-headed, low height, resistant to the broomrape races: E, F, G, has a good combining ability. The paternal line ED 193 is branched, also resistant to broomrape (races E, F, G) and three races of downy mildew (330, 710, and 730). The hybrid Status belongs to the middle-early maturity group, duration of the growing season is 95 days. The average plant height over the years of testing was 140 cm; the plants are uniformed in height and the duration of the development phases. Seed yield of the hybrid Status exceeded the standard one (a hybrid Gorstar) by 0.25 t/ha, and oil yield – by 0.14 t/ha. The oil content of the seeds was 48.0%, the huskiness – 28.7%, the 1000 seeds weight – 60.6 g. The new hybrid is resistant to seven races of broomrape (A, B, C, D, E, F, and G) and three races of downy mildew (330, 710, and 730). A distinctive feature of it is resistance of both parent lines to broomrape. The originator of a simple interline sunflower hybrid Status is V.S. Pustovoit All-Russian Research Institute of Oil Crops.

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