scholarly journals Thrombolytic Potentials of Some Medicinal Plants Used by the Local People for Cardiovascular Diseases in Bangladesh

2021 ◽  
Vol 28 (2) ◽  
pp. 405-412
Author(s):  
Mohammad Zashim Uddin ◽  
Atiya Begum Rifat ◽  
Farhana Yesmin Mitu ◽  
Tahmina Haque ◽  
Md Abdul Mazid
Keyword(s):  
Cardiovascular Diseases ◽  
Medicinal Plants ◽  
Animal Studies ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Clot Lysis ◽  
Terminalia Arjuna ◽  
Thrombolytic Activity ◽  
Standard Drug

Cardiovascular diseases (CVDs) are one of the major causes of death in the world. Medicinal plants with thrombolytic properties may be used as an alternative to modern medicines for CVDs. The present study was aimed to evaluate the thrombolytic potential of six medicinal plants available in Bangladesh using an in vitro clot lysis method where streptokinase and ethanol were used as a positive and negative control, respectively. Ethanolic extract at a dose of 10 mg/ml of Arjun tree (Terminalia arjuna), Garlic (Allium sativum), Elephant apple (Dillenia indica), Amla (Phyllanthus emblica), Yellow mombin (Spondias pinnata) and Burmese grape (Baccaurea ramiflora) showed 14.18 ± 1.23%, 10.72 ± 0.78%, 8.25 ± 0.42%, 7.08 ± 0.64%, 5.42 ± 0.47% and 2.47 ± 0.19% clot lysis, respectively, whereas the standard drug streptokinase lysed 41.11±0.31% clot at a dose of 30,000 IU. From the data, it is evident that ethanolic extracts of six selected medicinal plants possess a moderate to insignificant thrombolytic activities. Among these plants, Arjun tree and Garlic exhibited the highest thrombolytic activity and the Burmese grape showed the lowest thrombolytic activity. Through our study, it could be concluded that Arjun tree, Garlic, and Elephant apple might be used as traditional healing purposes of CVDs. However, further animal studies will prove the scientific justification of their uses. Conservation efforts should be given for Arjun tree, Elephant apple, Yellow mombin, Burmese grape, and Amla to save these plants from extinction in nature. Bangladesh J. Plant Taxon. 28(2): 405-412, 2021 (December)

scholarly journals Ascertainment of In vivo Antidiarrheal and In vitro Thrombolytic Effect of Ethanolic Extract of Leaves of Amomum dealbatum

2019 ◽  
Author(s):  
Md. Azimul Islam ◽  
Mohammed Aktar Sayeed ◽  
Md. Abdul Barek ◽  
Enama Nabi Shetu ◽  
Md. Nurul Faisal
Keyword(s):  
Castor Oil ◽  
Venous Blood ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Clot Lysis ◽  
Thrombolytic Activity ◽  
Thrombolytic Effect

Aims: The present study aimed to investigate antidiarrheal and thrombolytic effect of ethanolic extract of leaves of A. dealbatum in mice. Study design: Antidiarrheal effect was evaluated by castor oil-induced diarrhea method at two different concentrations in mice and in vitro thrombolytic activity was analyzed with clot lysis assay of human blood. Place and duration of study: Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh, between December 2018 and February 2019. Methodology: The male Swiss mice’s were divided into four groups (n = 5). First group was orally treated with 1% Tween-80 (10 ml/kg) and second group was orally treated with loperamide (5 mg/kg). Third and fourth group were orally treated with ethanolic extract of leaves of A. dealbatum at 200 and 400 mg/kg accordingly. Human RBCs were collected for conducting thrombolytic assay. During this study, 1.5 ml of venous blood was drawn from healthy volunteers (n = 10) and Streptokinase was employed as positive control and distilled water was employed as negative control. Results: In castor oil induced diarrhea model, ethanolic extract of leaves of A. dealbatum at 200, 400 mg/kg and loperamide (5 mg/kg) significantly reduced the number of feces and increase percent of inhibition of defecations compared to negative control. The extract showed percent of inhibition of defecation of 16.67 and 37.50 for 200 and 400 mg/ml respectively where the positive control loperamide showed 66.67%. Percentage of clot disruptions were 4.51 (p<.001), 75.69 (p<.001) and 26.07 (p<.001) for water, streptokinase and 10 mg/ml extract respectively. Conclusion: Based on the results from in vivo and in vitro activities, the leaves of A. dealbatum were found to be a potential source of new antidiarrheal and thrombolytic agents.

scholarly journals Ethanolic extract of ocimum kilimandscharicum linnleaves: phytochemical and in vitro pharmacological activity

2021 ◽  
pp. 106-109
Author(s):  
Yamini N ◽  
Lahari S ◽  
Phani deepthi V
Keyword(s):  
In Vitro Model ◽  
Ethanolic Extract ◽  
Clinical Development ◽  
Dependent Manner ◽  
Thrombolytic Activity ◽  
Standard Drug ◽  
Ethanolic Extracts ◽  
Vitro Model ◽  
Dose Dependent

Using an in vitro model, the anti-thrombolytic efficacy of ethanolic extracts of Ocimum kilimandscharicum Linn was investigated. The researchers discovered that different concentrations of the extract had significant anti-thrombolytic activity in a dose-dependent manner , which was comparable to a standard drug. As a result of the presence of flavonoids and polyphenols in the plant extract, it can be concluded that it has a promising future in the treatment of thrombosis. This knowledge will be useful in the clinical development of thrombolytic therapeutics by identifying more potent anti-thrombolytic principles from natural resources..    

scholarly journals Thrombolytic Activity of Methanolic Extracts of Desmodium paniculatum (L.) and Sarcochlamys pulcherrima (Roxb.)

2015 ◽  
Vol 17 (1) ◽  
pp. 67-69 ◽  
Author(s):  
Mohammed Aktar Sayeed ◽  
Humayun Kabir ◽  
Mohammad Mamun Ur Rashid ◽  
Md Farid Ahamad Bhuiyan ◽  
Mohammad A Rashid
Keyword(s):  
Pharmaceutical Journal ◽  
Positive Control ◽  
Negative Control ◽  
Clot Lysis ◽  
Herbal Extracts ◽  
Thrombolytic Activity ◽  
Methanolic Extracts ◽  
Desmodium Paniculatum

An in vitro thrombolytic model was used to check the clot lysis effect of two herbal extracts viz., Desmodium paniculatum (L.) and Sarchochlamys pulcherrima (Roxb.) by using Streptokinase as positive control and water as negative control. D. paniculatum and S. pulcherrima showed 31.92 ± 8.09% and 36.12 ± 6.81% clot lysis, respectively. From our study we found that D. paniculatum and S. pulcherrima showed significant % of clot lysis effect with reference to Streptokinase (72.54 ± 6.03%). and water (3.48 ± 0.84%). DOI: http://dx.doi.org/10.3329/bpj.v17i1.22318 Bangladesh Pharmaceutical Journal 17(1): 67-69, 2014

scholarly journals In silico and antithrombotic studies of 1,3,4-oxadiazoles derived from benzimidazole

2015 ◽  
Vol 11 (1) ◽  
pp. 67 ◽  
Author(s):  
B. Vishwanathan ◽  
B. M. Gurupadayya ◽  
K. Venkata Sairam
Keyword(s):  
In Silico ◽  
Factor Xa ◽  
Docking Studies ◽  
Negative Control ◽  
Clot Lysis ◽  
Drug Candidate ◽  
Thrombolytic Activity ◽  
Reference Drug ◽  
Oxadiazole Derivatives

<p class="Abstract">In the present study, a series of 1,3,4-oxadiazole derivatives (4a-4k) derived from benzimidazole were docked onto factor Xa (PDB: 1NFY) protein using SYBYLX 2.1. and also evaluated for <em>in vitro</em> clot lysis for thrombolytic activity. The synthesized molecules were also screened for in silico ADME studies. The molecular docking studies highlighted that the molecules showed high affinity towards 1NFY with higher docking score and the <em>in silico</em> ADME results were promising and indicated that the molecules holds great potential as a drug candidate. The thrombolytic evaluation was performed for decrease in solid clot weight by the clot lysis study at a concentration of 6.25, 12.5 and 25 µM strengths, respectively. The results of in vitro clot lysis for thrombolytic evaluation revealed that the tested compounds 4a-4k exhibited significant clot lysis with respect to negative control phosphate buffered saline and in comparison to the reference drug streptokinase (30,000 IU). Among all the tested compounds, compound 4j, 4d and 4g exhibited potent thrombolytic activity with EC<sub>50</sub> value of 16.2, 18.1 and 23.7 µM, respectively. The thrombolytic efficacy investigation highlights that the synthesized compound 4j could be considered for further clinical studies to ascertain its possible hit as thrombolytic agents.</p><p> </p>

scholarly journals In vitro Thrombolytic and Membrane Stabilizing Studies of Brassica rapa subsp. chinensis (L.) Hanelt

2015 ◽  
Vol 17 (2) ◽  
pp. 172-176 ◽  
Author(s):  
Nushrat Jahan ◽  
SM Ashikur Rahman ◽  
Mohammad S Rahman ◽  
Mohammad A Rashid
Keyword(s):  
Carbon Tetrachloride ◽  
Brassica Rapa ◽  
Methanol Extract ◽  
Pharmaceutical Journal ◽  
Negative Control ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Standard Drug ◽  
Crude Methanol Extract

Brassica rapa subsp. chinensis (L.) Hanelt is a popular herb in Bangladesh. In the present studies, the plant has been assessed to evaluate its in vitro thrombolytic and membrane stabilizing properties. Initially, the plant was collected, processed and extracted with methanol and the concentrated crude methanol extract was fractionated into petroleum ether, carbon tetrachloride, chloroform and aqueous soluble materials. All extractives, including the parent extract were subjected to thrombolytic and membrane stabilizing bioassays. In case of thrombolytic study, the carbon tetrachloride soluble fraction exhibited highest clot lysis. Here, streptokinase and distilled water were used as positive and negative control, respectively. However, in the assay for membrane stabilizing activity, the crude methanol extract along with other extractives were capable to inhibit hemolysis of erythrocyte membrane in hypotonic solution- and heat- induced conditions, which indicated anti-inflammatory property of the samples. Acetyl salicylic acid was used as standard drug in this assay. This represents the first report of thrombolytic and membrane stabilizing activities of B. rapa subsp. chinensis. DOI: http://dx.doi.org/10.3329/bpj.v17i2.22336 Bangladesh Pharmaceutical Journal 17(2): 172-176, 2014

scholarly journals Evaluation of ex vivo Thrombolytic Activity and in vitro Anti-inflammatory Activity of Thespesia populnea Leaf Extract

2017 ◽  
Vol 9 (05) ◽  
Author(s):  
Vineela Satuluri ◽  
Vidyadhara Suryadevara ◽  
Vijetha Pendyala ◽  
Narasimhareddy M.
Keyword(s):  
Leaf Extract ◽  
Inflammatory Activity ◽  
Clot Lysis ◽  
Dependent Manner ◽  
Thrombolytic Activity ◽  
Standard Drug ◽  
Alcohol Extract ◽  
Thespesia Populnea ◽  
Anti Inflammatory

Thrombotic disorders like myocardial and cerebral infarction are fatal blood clotting related diseases. Synthetic therapeutics used in such disorders has serious adverse effects, so there is a need to investigate some more safe natural thrombolytic agents. Present study is a preliminary work towards such endeavors. During this study analysis of thrombolytic activity and anti-inflammatory activity of Thespesia populnea leaf extract using a simple and quick in vitro clot lysis assay was performed. Various concentrations of leaf extract i.e. 200μg/ml; 400μg/ml and 600μg/ml were tested at various time intervals including; 24, 48 and 72 hours duration of incubation at 37°C for observing maximum clot lysis. The result findings indicated that concentrations of leaf extract enhanced the percentage of clot lysis in dose dependent manner along with the incubation time factor. However; streptokinase SK a reference standard and water were used as a positive and negative control showed clot lysis maximum 96.35% and 35.22% in 72 hours of incubation respectively. Alcohol extract of whole plant of Thespesia populnea (Family: Malvaceae) was assessed for its anti-inflammatory activity by in vitro methods. In vitro anti-inflammatory activity was evaluated using albumin denaturation assay at different concentrations. Diclofenac sodium was used as standard drug. The results showed that Thespesia populnea alcohol extract at a concentration range of 400-1600μl significantly protects from protein denaturation.

scholarly journals Molecular Docking, Computational and Antithrombotic Studies of Novel 1,3,4-oxadiazole Derivatives

2018 ◽  
Author(s):  
Majda Batool ◽  
Affifa Tajammal ◽  
Firdous Farhat ◽  
Francis Verpoort ◽  
Zafar A. K. Khattak ◽  
...  
Keyword(s):  
Factor Xa ◽  
Negative Control ◽  
Clot Lysis ◽  
Standard Drug ◽  
Reference Drug ◽  
Docking Score ◽  
Oxadiazole Derivatives ◽  
Inhibitory Potential

A new series of 1,3,4-oxadiazoles derivatives was synthesized, characterized and evaluated for their in vitro and in vivo anti-thrombotic activity. Compounds (3a-3i) exhibited significant clot lysis with respect to negative control and reference drug streptokinase (30,000 IU) while enhanced clotting time (CT) values were observed (130-342 sec) for these tested compounds than the standard drug heparin (110 sec.). High affinity towards 1NFY with greater docking score was observed for the compounds (3a, 3i, 3e, 3d and 3h) than the control ligand RPR200095. In addition, very good inhibitory potential against factor Xa (F-Xa) was observed with higher docking scores (5612-6270) with ACE values (&ndash;189.68 to &ndash;352.28 kcal/mol) than the control ligand RPR200095 (Docking score 5192; ACE &ndash;197.81 kcal/mol. In vitro, in vivo and in silico results proposed that these newly synthesized compounds can be used as anti-coagulant agents.

scholarly journals Bioactivities and Chemical Profiling of Sesbania grandiflora (L.) Poir. Leaves Growing in Bangladesh

2017 ◽  
Vol 15 (2) ◽  
pp. 173-176 ◽  
Author(s):  
Farhina Rahman Laboni ◽  
Samira Karim ◽  
Jalal Uddin ◽  
Zubair Khalid Labu
Keyword(s):  
Ethyl Acetate ◽  
Soluble Fraction ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Negative Control ◽  
Clot Lysis ◽  
Significant Percentage ◽  
Chemical Profiling ◽  
Sesbania Grandiflora

The ethanolic extract of leaves of Sesbania grandiflora and their organic and aqueous soluble partitioning substances were evaluated for thrombolytic and membrane stabilizing potentials in vitro. In thrombolytic assay, among all partitionates, the ethyl acetate soluble fraction showed highest percent of clot lysis (59.57%) as compared to 69.23% and 3.07% exhibited by the standard streptokinase and water (negative control), respectively. With respect to the membrane stabilizing activity, ethyl acetate soluble fractions of S. grandiflora also profoundly inhibited the hemolysis of erythrocytes induced by osmosis (64.30 ± 0.64%) and heat (57.21 ± 0.69%), respectively. In contrast, standard acetyl salicylic acid resulted in 70.12 ± 26% inhibition of osmotically-induced hemolysis and slightly higher level of protection against heat-induced hemolysis (73.90 ± 0.29%). The ethanol extract of leaves of S. grandiflora revealed significant percentage of thrombolytic and membrane stabilizing activities.Dhaka Univ. J. Pharm. Sci. 15(2): 173-176, 2016 (December)

scholarly journals Optimal Initial Positioning of Chest Tubes to Prevent Retained Hemothorax Using a Novel Steerable Chest Tube With Extendable Infusion Cannula

2021 ◽  
Vol 186 (Supplement_1) ◽  
pp. 324-330
Author(s):  
John B Fortune ◽  
Serena Murphy ◽  
Kimberley Tiller
Keyword(s):  
Chest Tube ◽  
Animal Studies ◽  
Training Session ◽  
Penetrating Trauma ◽  
Pleural Space ◽  
Tube Placement ◽  
Clot Lysis ◽  
Retained Hemothorax ◽  
Tip Position

ABSTRACT Introduction With blunt and penetrating trauma to the chest, warfighters and civilians frequently suffer from punctured lung (pneumothorax) and/or bleeding into the pleural space (hemothorax). Optimal management of this condition requires the rapid placement of a chest tube to evacuate as much of the blood and air as possible. Incomplete drainage of blood leading to retained hemothorax may be the result of the final tube tip position not being in contact with the blood collections. To address this problem, we sought to develop a “steerable” chest tube that could be accurately placed or repositioned into a specific desired position in the pleural space to assure optimal drainage. An integrated infusion cannula was added for the instillation of anticoagulants to maintain tube patency, thrombolytics for clot lysis, and analgesics for pain control if required. Materials and Methods A triple-lumen tube was designed to provide a channel for a pull-wire which was wound around an axle integrated into a small proximal handle and controlled by a ratcheted thumbwheel. Tension on the wire creates an arc on the tube that allows for positioning. In vitro testing focused on the relationship between the tension on the pull-wire and the resultant arc. Two adult cadavers and two anesthetized pigs were used to study the feasibility of accurate tube placement. After a brief training session, providers were asked to place tubes inferiorly along the diaphragm where blood was anticipated to accumulate or at the apex of the lung for pneumothorax. Success was determined with fluoroscopic images and was judged as a tube tip lying in the targeted position. Results The design was prototyped with an extruded polyvinyl chloride multilumen tube and a 3D printed tensioning handle. In vitro studies showed that one turn of the thumbwheel created 70° to 90° of arc of the tube. Cadaver and animal studies showed consistent success in the desired placement of the tube at or near the lateral diaphragm or in the apex. Attempts were also successful by surgical residents with minimal training. Conclusions Initial preliminary studies on a novel steerable chest tube have demonstrated the ability to appropriately position the tube in a desired location. The addition of an extendable cannula will allow for safe clot lysis or maintained tube patency. Additional studies are planned to confirm the benefit of this device in preventing retained hemothorax.

scholarly journals Exploration of in vitro thrombolytic, anthelminthic, cytotoxic and in vivo anxiolytic potentials with phytochemical screening of flowers of Brassica nigra

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Mohammad Sarowar Uddin ◽  
Md. Shalahuddin Millat ◽  
Mohammad Safiqul Islam ◽  
Md. Saddam Hussain ◽  
Md. Giash Uddin ◽  
...  
Keyword(s):  
Brassica Nigra ◽  
Anxiolytic Activity ◽  
Clot Lysis ◽  
Dependent Manner ◽  
Standard Drug ◽  
Lead Compounds ◽  
Lysis Activity ◽  
Test Models

Abstract Background Brassica nigra is a plant of Brassicaceae family, which possesses numerous medicinal values. Our present study is intended to assess the potential in vitro thrombolytic, anthelminthic, cytotoxic and in vivo anxiolytic properties of MCE of B. nigra flowers. MCE was fractioned for separating the compound on the basis of polarity by using chloroform, n-hexane and ethyl acetate solvent. Thrombolytic and anthelminthic activities were explained by collecting human erythrocytes and earthworms as test models, respectively. Anxiolytic activity was evaluated by elevated plus maze and hole board models while cytotoxic test was conducted through brine shrimp lethality bioassay. Results MCE revealed the presence of alkaloids, flavonoids, tannin, diterpenes, glycosides, carbohydrates, phenols, fixed oils and fat. In case of thrombolytic test, the MCE, CSF, ASF and n-HSF had produced maximum clot lysis activity at 5 and 10 mg/ml dose conditions. Two different concentrations (10 and 20 mg/ml) of MCE and its fractions showed significant (p < 0.05) anthelminthic activities in a dose-dependent manner. Significant anxiolytic activity was observed for all fractions which was comparable to the standard drug diazepam (p < 0.05). Again, the cytotoxic screening also presented good potentials for all fractions. Conclusion From the findings of present study, we can conclude that MCE of B. nigra flowers and its fraction possess significant anxiolytic, anthelmintic, anticancer and thrombolytic properties which may be a good candidate for treating these diseases through the determination of bio-active lead compounds.

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