scholarly journals Preparation and Characterization of Anti-CeTNT-1 and Anti-CeTNT-4 Antibodies

1970 ◽  
Vol 44 (3) ◽  
pp. 371-376
Author(s):  
M Ziaul Amin ◽  
Hiroaki Kagawa ◽  
Mohammed A Satter
Keyword(s):  
Fusion Proteins ◽  
Tissue Specificity ◽  
Body Wall ◽  
Troponin T ◽  
Specific Interaction ◽  
The Body ◽  
Expression Vectors ◽  
Antibody Specificity ◽  
Sds Page

Among the four CeTNT isoforms, CeTNT-1, CeTNT-2 are body wall types, CeTNT-4 is pharynx type and CeTNT-3 is expressed in both the body wall and pharyngeal tissue. In our previous study, we used body wall and pharynx type anti-CeTNI, anti-CeTNC and anti-CeTM antibodies to observe the tissue specific interaction of the TNI isoforms with others TN subunits and tropomyosin isoforms. To extent the interaction study of CeTNT isoforms, in this study, we prepared and characterized the body wall type anti-CeTNT-1 and pharynx type anti- CeTNT-4 antibodies. For the preparation of the anti-CeTNT-1 and anti-CeTNT-4 antibodies, in this study we constructed the pCTNT-1 and pCTNT-4 expression vectors. The sub-cloned of the pCTNT-1 and pCTNT-4 expression vectors were verified by DNA sequencing. These expression vectors were used to generate fusion proteins of the body wall, TNT-1 and pharyngeal TNT-4 isoforms in Escherichia Ecoli. The expression of these fusion proteins were confirmed by SDS-PAGE analysis. The anti-CeTNT-1 and anti-CeTNT-4 antibodies were prepared in the rabbit by using the gel cut of the CeTNT-1 and CeTNT-4 fusion proteins. The antibody specificity of the CeTNT-1 and CeTNT-4 fusion proteins was also judged by Western-analysis using prepared anti-CeTNT-1 and anti-CeTNT-4 antibodies. The antibody specificity results indicated that anti-sera against each of both the body wall type TNT-1 and pharynx type TNT-4 isoforms had tissue specificity. Key words: Troponin T, Caenorhabditis elegans, Body wall, Pharynx DOI: 10.3329/bjsir.v44i3.4413 Bangladesh J. Sci. Ind. Res. 44(3), 371-376, 2009

Extraction and Characterization of Pepsin Soluble Collagen from the Body Wall of Sea Cucumber Acaudina leucoprocta

2017 ◽  
Vol 26 (5) ◽  
pp. 502-515 ◽  
Author(s):  
Saijun Lin ◽  
Ya-Ping Xue ◽  
Enli San ◽  
Tan Chee Keong ◽  
Lifang Chen ◽  
...  
Keyword(s):  
Sea Cucumber ◽  
Body Wall ◽  
The Body ◽  
Soluble Collagen ◽  
Pepsin Soluble Collagen

Fragile skeletal muscle attachments in dystrophic mutants of Caenorhabditis elegans: isolation and characterization of the mua genes

Development ◽  
2000 ◽  
Vol 127 (6) ◽  
pp. 1197-1207 ◽  
Author(s):  
J.D. Plenefisch ◽  
X. Zhu ◽  
E.M. Hedgecock
Keyword(s):  
Caenorhabditis Elegans ◽  
Body Wall ◽  
Local Strain ◽  
Muscle Differentiation ◽  
The Body ◽  
Tissue Separation ◽  
New Genes ◽  
Isolation And Characterization ◽  
Skin Fragility

Over 30 Caenorhabditis elegans mutants were identified with normal muscle differentiation and initial locomotion followed by catastrophic detachment of skeletal muscles from the body wall. Reducing the strength of muscle contraction in these mutants with a myosin gene mutation suppresses muscle detachment. These dystrophic mutants identify a novel class of genes required for growth and maintenance of functional muscle attachments, not exceptional alleles of genes required for muscle differentiation and contractility. Nine new genes, named mua, and two previously published loci, unc-23 and vab-10, cause fragile musscle attachments. The primary sites of muscle detachment, including the plane of tissue separation, are characteristic for each gene. We suggest these genes identify feedback mechanisms whereby local strain regulates the extent of myofibril contraction and the placement of new muscle attachments in functioning muscles. Finally, we draw some comparisons to vertebrate skin fragility diseases and muscular dystrophies.

Purification and partial characterization of an acid phosphatase from the body wall of sea cucumber Stichopus japonicus

2009 ◽  
Vol 44 (8) ◽  
pp. 875-879 ◽  
Author(s):  
Bei-Wei Zhu ◽  
Jian-Wei Yu ◽  
Zongshen Zhang ◽  
Da-Yong Zhou ◽  
Jing-Feng Yang ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Sea Cucumber ◽  
Body Wall ◽  
The Body ◽  
Partial Characterization ◽  
Stichopus Japonicus

scholarly journals Characterization of metallothionein protein from hepatopancreas organ of Pilsbryoconcha exilis collected from Cikaniki River, Western Java, Indonesia

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
SATA YOSHIDA SRIE RAHAYU ◽  
WAHYU PRIHATINI
Keyword(s):  
Stress Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
The Body ◽  
Freshwater Environment ◽  
Sds Page ◽  
Freshwater Biota ◽  
Aas Method ◽  
Endogenous Proteins

Abstract. Rahayu SYS, Prihatini W. 2020. Characterization of metallothionein protein from hepatopancreas organ of Pilsbryoconcha exilis collected from Cikaniki River, Western Java, Indonesia. Nusantara Bioscience 12: 1-5. Freshwater environment, undergoing various changes due to the presence of dangerous toxic anthropogenic waste. It causes pressure on the freshwater biota that lives in it, such as Pilsbryoconcha exilis mussel at the bottom of freshwater. This pressure is controlled by the body through the synthesis of a set of stress proteins. Endogenous proteins, metallothionein (MT), in the body of freshwater biota absorb heavy metals in the body of biota, in the form of stress control. This research identified MT protein on P. exilis from contaminated waters such as the Cikaniki river with the average of mercury levels in water, sediment, and hepatopancreas of mussels using AAS method were 0.001 mg/L, 0.120 mg/L, and 1.318 mg/L respectively. Hepatopancreas of P. exilis was extracted using a Tissue Extraction Reagent I kit (Invitrogen), with procedures following the factory manual. The extract was purified by filtration using Sephadec 50; then, the filtration results were migrated together with the PageRuler TM Unstained Low Range Protein Ladder (Fermentas) in Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS PAGE) gel medium on Biorad Protein II electrophoresis. After completion of electrophoresis, the gel was stained using Page Blue Protein Staining Solution (Fermentas), following the factory manual procedure. Characterization at this research has succeeded in obtaining the MT-I isoform protein measuring 5, 10, and 25 kDa from the hepatopancreas organ of P. exilis.

scholarly journals Release of Antioxidant Peptides from the Body Wall Proteins of the Sea Cucumber Isostichopus Fuscus

2015 ◽  
Vol 10 (8) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Arisaí C. Hernández-Sámano ◽  
Blanca Hernández-Ledesma
Keyword(s):  
Crude Protein ◽  
Sea Cucumber ◽  
Body Wall ◽  
Radical Scavenging ◽  
Marine Species ◽  
The Body ◽  
Antioxidant Peptides ◽  
Scavenging Capacity ◽  
Sds Page ◽  
Trolox Equivalent

Proteases from the tentacles of the sea cucumber Isostichopus fuscus were partially purified and used to produce antioxidant peptides from body wall proteins of this marine species. Three proteins (105, 68, and 39 kDa) were identified by SDS-PAGE in the proteolytic extract of the tentacles. Protein hydrolyzates were generated with gelatin and crude protein substrates from body wall, and peptidic fractions lower and higher than 3 kDa were obtained to evaluate their oxygen radical scavenging capacity (ORAC). The 3 kDa-fraction obtained from the crude protein hydrolyzate showed the highest ORAC value (0.92 ± 0.04 μmol Trolox equivalent/mg protein). This fraction was selected to purify peptides potentially responsible for the activity that might be used as ingredients for development of functional foods.

Neurochemical characterization of nervous elements innervating the body wall of earthworms (Lumbricus, Eisenia): immunohistochemical and pharmacological studies

2005 ◽  
Vol 321 (3) ◽  
pp. 479-490 ◽  
Author(s):  
Mária Csoknya ◽  
Boglárka Takács ◽  
Anna Koza ◽  
Viktória Dénes ◽  
Márta Wilhelm ◽  
...  
Keyword(s):  
Body Wall ◽  
The Body ◽  
Pharmacological Studies

Chemical characterization of saponins contained in the body wall and the Cuvierian tubules of the sea cucumber Holothuria (Platyperona) sanctori (Delle Chiaje, 1823)

2016 ◽  
Vol 68 ◽  
pp. 119-127 ◽  
Author(s):  
Guillaume Caulier ◽  
Karim Mezali ◽  
Dina L. Soualili ◽  
Corentin Decroo ◽  
Marie Demeyer ◽  
...  
Keyword(s):  
Sea Cucumber ◽  
Body Wall ◽  
Chemical Characterization ◽  
The Body
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