scholarly journals Pathogenic potentiality of fungi associated with the seeds of different cotton (Gossypium hirsutum L.) varieties in Bangladesh

2020 ◽  
Vol 29 (1) ◽  
pp. 107-115
Author(s):  
Amina Khatun ◽  
Shamim Shamsi ◽  
MA Bashar

A total of 24 species of fungi, namely Aspergillus flavus Link, A. fumigatus Fresenius, A. niger (Type 1 and Type 2) Van Tiegh, A. ochraceus K. Wilhelm, A. nidulans Eidam, Aspergillus sp.1, Aspergillus sp.2, Aspergillus sp.3, Curvularia lunata (Wakker) Boedijn, Colletotrichum gloeosporioides Penz & Sacc, C. gossypii Southw., Chaetomium globosum Kunze., Fusarium nivale (Fr.) Sorauer, F. moniliforme J. Shelden, F. oxysporum Schlechtendal, F. fujikuroi Nirenberg, Mucor sp. P. Micheli ex L., Penicillium sp.1 and sp.2 Link, Rhizoctonia solani Khun., Rhizopus stolonifer (Ehrenb.) Vuill., Rhizomucor sp. Lucet & Costantin, Syncephalastrum racemosum Cohn and Trichoderma viride Pers. were found to be associated with the seeds of 14 varieties (CB 1- CB 14) of cotton. Out of these 24 fungal species, nine were found to be pathogenic to cotton. They were Aspergillus flavus, A. niger (Type 1), Aspergillus sp. 1, Colletotrichum gloeosporioides, Curvularia lunata, Fusarium nivale, F. moniliforme, Mucor sp. and Rhizoctonia solani. These pathogenic fungi had remarkable effect on seed germination, vigor index, root-shoot length and mortality of cotton seedlings. The germination percentage of control seeds was 88 but because of the presence of pathogenic fungi the rate showed considerable reduction in all the varieties and it varied from 20 to 82%. Among the nine fungal isolates Rhizoctonia solani showed maximum reduction in seed germination. Aspergillus flavus, Colletotrichum gloeosporioides and Fusarium moniliforme also caused near about 50% reduction in seed germination. Mortality percentage of control seedling were also less (6) whereas, in inoculated seeds it was higher and varied from 7 - 23%. Root- shoot ratio of control seedlings was high but less in inoculated seedlings. The vigor index of control plant was high (1548.8) but less in inoculated plants. The lowest vigor index was noticed for Rhizoctonia solani (202.0) and highest for Aspergillus sp. 1(1213.6). Results indicated that Curvularia lunata and Rhizoctonia solani showed a greater impact in reduction of cotton seed germination and vigor index. Dhaka Univ. J. Biol. Sci. 29(1): 107-115, 2020 (January)

2020 ◽  
Vol 29 (2) ◽  
pp. 237-244
Author(s):  
Amina Khatun ◽  
Shamim Shamsi ◽  
MA Bashar

Seed samples with highest (CB10), lowest (CB8), moderate fungal frequency (CB3) and randomly selected seeds from all varieties (CB1-14) were used. A total of 14 species of fungi, namely Aspergillus flavus Link, A. fumigatus Fresenius, A. niger Van Tiegh, Curvularia lunata (Wakker) Boedijn, Colletotrichum gloeosporioides Penz & Sacc, Fusarium nivale (Fr.) Sorauer, F. oxysporum Schlechtendal, F. fujikuroi Nirenberg, Penicillium aculeatum Raper & Fennell, Penicillium citrinum Thom, Rhizoctonia solani Khun., Rhizopus stolonifer (Ehrenb.) Vuill., Syncephalastrum racemosum Cohn and Trichoderma viride Pers. were found to be associated with seed parts of different varieties of cotton. The maximum ten species of fungi were isolated from seed coat. The most prevalent fungus was Rhizopus stolonifer which was found in all the parts of all the varieties of cotton seeds examined. Out of the 14 isolated fungi, Aspergillus flavus, A. fumigatus, A. niger, C. lunata, C. gloeosporioides, F. nivale, F. oxysporum, F. fujikuroi, Penicillium aculeatum, R. solani, R. stolonifer, S. racemosum and T. viride were found to be external and Aspergillus flavus, A. fumigatus, A. niger, C. lunata, F. nivale, Penicillium citrinum and R. stolonifer were internal seed borne fungi. Aspergillus flavus, A. fumigatus, A. niger, C. lunata, F. nivale and R. stolonifer were isolated as both external and internal seed borne fungi. Dhaka Univ. J. Biol. Sci. 29(2): 237-244, 2020 (July)


2021 ◽  
Vol 5 (1) ◽  
pp. 86
Author(s):  
Grace Purnamasari Christian ◽  
Wahyu Irawati

<p>The post-harvest fungi is one of the greatest cause of various diseases. Particularly, in fungus that infects seeds, the toxic substance called aflatoxin is found. This has caused us to wonder: Has fungi been pathogenic fungi since the beginning of creation? The purpose of this research is to study: 1) The Place of Fungi in God’s Original Creation and the Origin of Pathogenic Fungi, 2) Characterization of post-harvest fungus on corn seeds, 3) Aspergillus sp.’s Structure as Proof of God’s Wonderful Original Creation and Providence, and 4) The factors that contribute to the growth of fungi in post-harvest corn seeds and our responsibility. We conclude that in the beginning, fungi were a part of God’s originally good and perfect creation. These fungi were created by God with the structure and function to support its operation in fulfilling God’s creative purpose. The complex structure of Aspergillus niger and Aspergillus flavus that infected the corn seeds showed God’s providence after the Fall. Pathogenic fungi were the result of the Fall of man into sin. Their growth is caused by the failure to give proper care to the crops. But this should not discourage us since the Bible tells us that God is continually working to restore His creation. Therefore, we are called to be stewards of His creation, to develop and preserve whatever is entrusted in our hands, including the crops for God’s glory and under His authority until He has fully renewed and restored everything.</p><p><strong>BAHASA INDONESIA ABSTRACT:</strong> Jamur pasca panen merupakan salah satu penyebab terbesar timbulnya berbagai penyakit, khususnya disebabkan oleh jamur yang menginfeksi biji-bijian dan menghasilkan substansi beracun disebut dengan aflatoksin. Keadaan ini menimbulkan pertanyaan apakah jamur patogenik ada sejak awal penciptaan? Penelitian ini bertujuan untuk mempelajari: 1) Posisi jamur patogenik di awal penciptaan dan asal mula jamur patogenik, 2) karakteristik jamur pasca panen pada biji jagung, 3) struktur <em>Aspergillus</em> sp sebagai bukti dari providensi Allah akan ciptaanNya yang sangat luar biasa, 4) faktor-faktor yang berkontribusi terhadap pertumbuhan jamur pasca panen biji jagung dan Tanggung jawab kita. Dari hasil penelitian ini disimpulkan bahwa pada mulanya, jamur merupakan bagian ciptaan Allah yang sungguh amat baik dan sempurna. Struktur yang kompleks dari <em>Aspergillus niger</em> dan <em>Aspergillus flavus </em>yang menginfeksi biji jagung menunjukkan providensi Allah setelah kejatuhan dosa. Pertumbuhan jamur patogenik merupakan gambaran kejatuhan manusia ke dalam dosa yaitu kegagalan manusia untuk memberikan pemeliharaan yang memadahi pada biji-bijian pasca panen. Namun demikian, keadaan ini tidak mematahkan semangat kita karena Alkitab mengatakan bahwa Allah masih terus bekerja untuk memulihkan ciptaanNya. Oleh karena itu, diharapkan kita semua menjadi pelayan atas ciptaanNya untuk mengembangkan dan menjaga apa yang dipercayakan kepada kita di bawah otoritasNya termasuk biji-bijian untuk memuliakan Allah hingga Dia secara penuh memperbaharui dan memulihkan segala sesuatu</p>


2020 ◽  
Vol 3 (1) ◽  
pp. 1-15
Author(s):  
Sifa Sobianti ◽  
Loekas Soesanto ◽  
Suciati Hadi

Penelitian ini bertujuan untuk mengetahui (1) beragam spesies jamur patogen tular-benih pada lima varietas padi, dan (2) persentase daya kecambah lima varietas padi. Penelitian dilaksanakan di Laboratorium Karantina Pertanian Kelas I Semarang, dari bulan Maret sampai dengan Mei 2019. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 5 perlakuan dan 5 ulangan, perlakuan terdiri atas Varietas Inpago Unsoed 1, Inpago Unsoed Parimas, IR64, Ciherang, dan Situ Bagendit. Isolasi dan identifikasi jamur patogen tular-benih dilakukan pada blotter test dan medium PDA, dengan pengamatan di bawah mikroskop stereo dan kompon, kemudian dibandingkan dengan pustaka. Variabel yang diamati yaitu morfologi koloni patogen, morfologi patogen, dan daya kecambah. Hasil penelitian menunjukkan bahwa (1) pada semua varietas benih padi terdapat jamur patogen tular-benih, tetapi tidak semua jamur tersebut dijumpai pada setiap varietas. Jenis jamur patogen tular-benih yang dijumpai, yaitu Alternaria padwickii Ganguly, Aspergillus flavus Link, Aspergillus niger van Tieghem, Curvularia lunata (Wakker) Boedjin, Curvularia pallescens Boedjin, Drechslera oryzae Breda de Haan, Fusarium semitectum Berk. & Rav. [W&R, G,B,J], Rhizoctonia solani J. G. Kuhn, Rhizopus oryzae Went & Prins. Geerl., dan Tilletia barclayana Bref., dan (2) uji daya kecambah menunjukkan bahwa masing-masing varietas benih padi memilki persentase daya kecambah benih yang berbeda. Daya kecambah benih berturut-turut pada varietas IR64, Ciherang, Inpago Unsoed 1, Inpago Unsoed Parimas, dan Situ Bagendit sebesar 85,75, 81,75, 80,25, 76,5, dan 70%.


2020 ◽  
Vol 21 (9) ◽  
Author(s):  
Loekas Soesanto ◽  
Annisa Rahmaddila Rizky Hartono ◽  
Endang Mugiastuti ◽  
Heri Widarta

Abstract. Soesanto L, Hartono ARR, Mugiastuti E, Widarta H. 2020. Seed-borne pathogenic fungi on some soybean varieties. Biodiversitas 21: 4010-4015. The present study was conducted to detect and identify seed-borne pathogenic fungi in some soybean varieties and their effect on seed germination. Experiment was performed in a completely random design with eight treatments and four replicates. Eight soybean varieties i.e., Malabar, Kaba, Dering, Detam I, Sinabung, Dena, Gepak Kuning, and Slamet were selected for the investigation. Seed borne fungi were isolated using blotter test and agar plate techniques. The variables observed were morphological/cultural characteristics, microscopic features of fungi, and percentage of seed germination. A total of eight fungi namely Aspergillus flavus Link, Aspergillus niger van Tieghem, Cladosporium oxysporum Berk. & M.A. Curtis, Colletotrichum dematium (Pers. et Fr.) Grove f.sp. truncate (Schw.) Arx, Curvularia pallescens Boedijn, Fusarium solani (Mart.) Sacc., Melanospora zamiae Corda, and Nigrospora sp. Mason were isolated from different varieties of soybean seeds. The highest seed germination was found to be 80.5% in Daring and Detam I varieties, respectively, and the lowest 53% was recorded in Dena variety.


2018 ◽  
Vol 10 (2) ◽  
pp. 45-54
Author(s):  
MD Hossain ◽  
M Imran ◽  
KA Bhuiyan ◽  
ME Habib ◽  
M Ikrum

A total of 20 chickpea seed samples were collected from BARI, Gazipurand different locations of Savar, Dhaka district. Blotter method was used for detection of the associated fungi of chickpea seeds. Altogether 14 fungi comprising 12 genera namely Aspergillus flavus, Aspergillus niger, Aspergillus sp, Botryodiplodia theobromae, Botryti cinerea, Curvularia lunata, Chaetomium globosum, Cladosporium sp., Colletotrichum dematium, Fusarium oxysporum, Macrophomina phasaelina, Rhizoctonia solani, Rhizopus stolonifer and Stemphylium sarciniforme were isolated from chickpea seed samples. Among the fungi isolated fungi, F. oxysporumwas most prevalent with an average incidence of 18.95% and found in all the seed samples. The germination of seed samples varied from 55-90 % on blotter. The pathogenic fungi and other storage fungi like Aspergillus caused lower the germination of the seeds.A pathogenicity test was conducted with 20 isolates of F. oxysporum against their origin of chickpea seed samples in pot culture. The pathogenecity ranged from weak to highly pathogenic. The isolates FO 19, FO 17, FO 11 and FO 18 were highly virulent. The isolates FO 9, FO10 and FO 15 were virulent and rest of the isolates were moderately virulent. The isolates FO 2 and FO 3 were weak pathogen.J. Environ. Sci. & Natural Resources, 10(2): 45-54 2017


Author(s):  
S. Apuri ◽  
C. Kwoseh ◽  
E. A. Seweh ◽  
I. O. Tutu

Aims: The study aims to identifying the microorganisms associated with post-harvest rot of frafra potatoes in Bongo-soe, Upper east region of Ghana. Place and Duration of Study: Department of Horticulture and the Pathology laboratory of the Faculty of Agriculture, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana. The Solenostemon rotundifolius tubers were stored at the Horticulture Department laboratory whiles rot identification was carried out at the Pathology laboratory of the Faculty of Agriculture. The Tubers were stored from 2nd November, 2012 to 22nd March 2013. Methodology: Four hundred (400) tubers of black cultivar and four hundred (400) tubers of a brown cultivar of Solenostemon rotundifolius tubers showing visible signs of rot during the storage were collected. Pieces of diseased tissues from the margin of the necrotic collected and immersed in 10% commercial bleach solution for sterilisation, for one minute. These were then blotted dry and plated on Potato Dextrose Agar PDA. The plates were sealed with a cellotape until growth occurred. Results: The microorganisms identified to be responsible for causing rot in Solenostemon rotundifolius tubers were six in number. Colletotrichum gloeosporioides was identified to be responsible for 30.76% of rots observed, followed by Aspergillus niger, 23.07%, Curvularia lunata, 19.23%, Aspergillus flavus, 11.54%, Trichoderma sp and Penicillium sp both recorded 7.70% of rots observed. The percentage incidence of Aspergillus niger (15.38%), Curvularia lunata (11.54%) and Aspergillus flavus (7.69%) was higher in the black cultivar as compared with the brown cultivar which had percentage incidence of 7.69%, 7.69% and 3.85% respectively. Also, the percentage incidence of Colletotrichum gloeosporioides (15.38%) and Penicillium sp (3.85%) was the same in both the black and brown cultivars of Solenostemon rotundifolius tubers used in this study. Conclusion: The activities of the damaging microorganisms can be reduced by controlling mechanical injury during harvesting, transportation and storage of Solenostemon rotundifolius tubers should be prevented or reduced because they pave the way for tuber infection by the rot causing microorganisms.


2020 ◽  
Vol 27 (1) ◽  
pp. 129-136
Author(s):  
Zuhra Yasmin ◽  
Shamim Shamsi

Fifteen fungal species representing 12 genera were found to be associated with leaf spot and anthracnose diseases of Rauvolfia serpentina (L.) Benth. ex Kurz. which were collected from different habitats of Bangladesh. Fungi were isolated following ‘tissue planting’ and ‘blotter’ method. The isolated fungi were Alternaria alternata (Fr.) Keissler, Aspergillus flavus Link ex Fr., A. niger van Tieghm, Colletotrichum gloeosporioides (Penz.) Sacc., Curvularia lunata (Wakker) Boedijn, Fusarium sp.1. Fusarium sp. 2, Macrophoma sp., Nigrospora sphaerica (Sacc.) Mason, Penicillium digitatum Saccardo, Penicillium italicum Whemer, Pestalotiopsis guepinii (Desm.) Stay, Pseudocercospora libenbergii (Syd.) Deighton, Rhizopus stolonifera Vuillemin. Toney, Trichoderma viride Pers. During the present investigation, Pseudocercospora libenbergii is a new record for Bangladesh. Bangladesh J. Plant Taxon. 27(1): 129-136, 2020 (June)


2019 ◽  
Vol 29 (2) ◽  
pp. 649
Author(s):  
Andrezza Klyvia Oliveira Araújo ◽  
Maria Lúcia Maurício Silva ◽  
Rommel Do Santos Siqueira Gomes ◽  
Angeline Maria da Silva Santos ◽  
Luciana Cordeiro do Nascimento

O objetivo da pesquisa foi avaliar a eficiência dos extratos de alho e erva-cidreira sobre patógenos e na qualidade fisiológica de sementes de Chorisia glaziovii. Os tratamentos constituíram-se de extratos de alho e erva-cidreira à 0, 25, 50, 75 e 100%, e fungicida Captan®. Para sanidade das sementes adotou-se o método blotter test e a qualidade fisiológica foi determinada pela germinação e vigor. O delineamento foi inteiramente casualizados em esquema fatorial (2x5) +1 (extratos vegetais x concentrações + fungicida), com quatro repetições. Observou-se a ocorrência de Aspergillus flavus, Aspergillus niger, Aspergillus sp., Botrytis sp., Cladosporium sp., Curvularia lunata, Nigrospora sp., Pestalotia sp., Periconia sp. e Rhizopus stolonifer, nas sementes. O extrato de erva-cidreira à 100% foi eficiente na inibição dos patógenos. O aumento na concentração do extrato de alho foi prejudicial à primeira contagem de germinação, e o extrato de erva-cidreira proporcionou aumento de sementes germinadas.


2018 ◽  
Vol 6 (2) ◽  
pp. 59-66
Author(s):  
Raihan I Raju

A number of seed borne fungi such as- Fusarium moniliforme, Curvularia lunata, Alternaria solani, Helminthosporium solani, Aspergillus flavus, A. niger and Penicillium spp. were identified from the selected BARI-8, Nilima, Patharkuchi, Roma and Ratan varieties of tomato seeds. The highest (2.53%) mean incidence of the fungi F. moniliforme were recorded in tomato variety Roma, BARI-8, Nilima and Ratan while the lowest (0.13%) mean incidence of the fungi Penicillium was found in Ratan. The maximum percent of germination (64%) was recorded in both BARI-8 and Patharkuchi varieties, while the minimum (7%) germination in Nilima. The highest seedling vigor index (632.96) was encountered in BARI-8 and the lowest in Nilima. Trichoderma viride, T. harzianum and T. koningii were used as bio-control agents against major seed borne fungi. T. viride showed the significantly effective mycelial growth inhibition against F. moniliforme and C. lunata, while T. harzianum and T. koningii showed poor performance against both tested fungi. In case of botanical extracts, Lawsonia inermis exhibited the maximum (82.15% and 86.24%) inhibition of mycelium growth of F. moniliforme and C. lunata, respectively. Chemical fungicides, Bavistin (0.5 gm/l) inhibited 65% mycelial growth against F. moniliforme, while higher concentration of chemical fungicide gave 81% inhibition of C. lunata. The results suggested that, L. inermis phytoextract can possibly be exploited for the management of seed-borne pathogenic fungi of tomato in an environmental friendly way.Jahangirnagar University J. Biol. Sci. 6(2): 59-66, 2017 (December)


2004 ◽  
Vol 29 (3) ◽  
pp. 332-335 ◽  
Author(s):  
Severina R. O. Lins ◽  
Rildo S. B. Coelho

As condições de cultivo das plantas ornamentais tropicais, relacionadas aos fatores precipitação, umidade, temperatura e densidade de plantio, favorecem a ocorrência de doenças que limitam a produção e reduzem a qualidade das flores. Destacaram-se as doenças causadas por fungos e nematóides, sendo assinaladas a antracnose (Colletotrichum gloeosporioides) em Heliconia spp., Etlingera elatior, Tapeinochilos ananassae, causando lesões em folhas e inflorescências; manchas foliares (Bipolaris spp., Cercospora sp., Curvularia lunata, Glomerella cingulata, Guignardia sp. e Deigthoniella torulosa) em Heliconia spp., Calathea burle marx e Musa coccinea; podridão de rizomas e raízes (Rhizoctonia solani e Fusarium oxysporum f. sp. cubense) em E. elatior e Heliconia chartacea cv. Sex Pink. As fitonematoses, causadas por espécies dos gêneros Meloidogyne, Radopholus e Helicotylenchus, constituem um dos principais problemas sanitários em ornamentais tropicais em Pernambuco, ocorrendo comumente em Alpinia purpurata, E. elatior, Zingiber espectabiles, Heliconia spp. e Musa spp. A espécie A. purpurata foi a mais suscetível a M. incognita. Em função dos trabalhos de erradicação pelos produtores, a murcha bacteriana (Ralstonia solanacearum raça 2) foi assinalada com baixa incidência nas áreas de cultivo de flores tropicais.


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