scholarly journals Evaluation of seed-borne fungi associated with tomato and their control measures

2018 ◽  
Vol 6 (2) ◽  
pp. 59-66
Author(s):  
Raihan I Raju
Keyword(s):  
Mycelial Growth ◽  
Trichoderma Viride ◽  
Poor Performance ◽  
Alternaria Solani ◽  
Pathogenic Fungi ◽  
Control Measures ◽  
Curvularia Lunata ◽  
Lawsonia Inermis ◽  
Mycelium Growth ◽  
Vigor Index

A number of seed borne fungi such as- Fusarium moniliforme, Curvularia lunata, Alternaria solani, Helminthosporium solani, Aspergillus flavus, A. niger and Penicillium spp. were identified from the selected BARI-8, Nilima, Patharkuchi, Roma and Ratan varieties of tomato seeds. The highest (2.53%) mean incidence of the fungi F. moniliforme were recorded in tomato variety Roma, BARI-8, Nilima and Ratan while the lowest (0.13%) mean incidence of the fungi Penicillium was found in Ratan. The maximum percent of germination (64%) was recorded in both BARI-8 and Patharkuchi varieties, while the minimum (7%) germination in Nilima. The highest seedling vigor index (632.96) was encountered in BARI-8 and the lowest in Nilima. Trichoderma viride, T. harzianum and T. koningii were used as bio-control agents against major seed borne fungi. T. viride showed the significantly effective mycelial growth inhibition against F. moniliforme and C. lunata, while T. harzianum and T. koningii showed poor performance against both tested fungi. In case of botanical extracts, Lawsonia inermis exhibited the maximum (82.15% and 86.24%) inhibition of mycelium growth of F. moniliforme and C. lunata, respectively. Chemical fungicides, Bavistin (0.5 gm/l) inhibited 65% mycelial growth against F. moniliforme, while higher concentration of chemical fungicide gave 81% inhibition of C. lunata. The results suggested that, L. inermis phytoextract can possibly be exploited for the management of seed-borne pathogenic fungi of tomato in an environmental friendly way.Jahangirnagar University J. Biol. Sci. 6(2): 59-66, 2017 (December)

scholarly journals Antifungal potential and biochemical effects of monoterpenes and phenylpropenes on plant

2017 ◽  
Vol 54 (No. 1) ◽  
pp. 9-16 ◽  
Author(s):  
Marei Gehan I Kh ◽  
Abdelgaleil Samir A M
Keyword(s):  
Antifungal Activity ◽  
Growth Inhibition ◽  
Mycelial Growth ◽  
Alternaria Solani ◽  
Pathogenic Fungi ◽  
Separate Experiment ◽  
Inhibitory Effects ◽  
Growth Inhibition Assay ◽  
Biochemical Effects ◽  
Antifungal Potential

To develop new natural fungicides, six monoterpenes and two phenylpropenes were tested for their antifungal activity against eight plant pathogenic fungi. The results of the mycelial growth inhibition assay showed that trans-cinnamaldehyde was the most potent compound against the eight tested fungi with EC<sub>50</sub> values ranging between 0.75 and 3.19 mg/l. This compound caused the higher mycelial growth inhibition than carbendazim. Furthermore, (–)-menthone exhibited strong antifungal activity against Alternaria solani (EC<sub>50</sub> = 9.31 mg/l), Penicillium digitatum (EC<sub>50</sub> = 16.14 mg/l), and Rhizoctonia solani (EC<sub>50</sub> = 24.69 mg/l). Likewise, eugenol showed potent antifungal activity against P. digitatum, R. solani, Fusarium solani, and A. solani, whereas EC<sub>50</sub> values were less than 30.0 mg/l. In a separate experiment, trans-cinnamaldehyde, p-cymene, eugenol, and (–)-menthone were evaluated for their inhibitory effects on pectin methyl esterase and cellulase. The tested compounds exhibited the pronounced inhibition of enzyme activities with trans-cinnamaldehyde being the most potent inhibitor for both enzymes.

scholarly journals Pathogenic potentiality of fungi isolated from seeds of three hill cotton varieties (Gossypium arboreum L.)

2019 ◽  
Vol 28 (2) ◽  
pp. 187-193
Author(s):  
Shamim Shamsi ◽  
Mst Naznin Nahar
Keyword(s):  
Rhizoctonia Solani ◽  
Trichoderma Viride ◽  
Pathogenic Fungi ◽  
Curvularia Lunata ◽  
Type I ◽  
Type Ii ◽  
Gossypium Arboreum ◽  
Rhizopus Stolonifer ◽  
Remarkable Effect ◽  
Seed Inoculation

Twelve species of fungi, namely Aspergillus flavus Link., A. fumigatus Fresenius., A. niger van Tiegh (Type-I)., A. niger van Tiegh (Type-II), Chaetomium globosum Kunze ex Fr., Curvularia lunata (Wakker) Boedijn, Fusarium moniliforme var. subglutinans Wr. & Reink, F. sporotrichioides Sherb., Mem., Penicillium Link., Pestalotiopsis guepinii (Desm.) Stay., Rhizoctonia solani J.G. Kühn, Rhizopus stolonifer (Ehrenb.: Fr.) Vuill and Trichoderma viride Pers were isolated from the seeds of three cotton varieties (HC-1, HC-2 and HC-3) following “Tissue planting” and “Blotter” methods. Among the isolated fungi, six, namely A. flavus, A. niger, C. lunata, F. moniliforme var. subglutinans, F. sporotrichioides and Rhizoctonia solani showed pathogenic potentiality following seed inoculation technique. These pathogenic fungi had remarkable effect on seed germination, root shoot length and mortality of cotton seedlings. Dhaka Univ. J. Biol. Sci. 28(2): 187-193, 2019 (July)

scholarly journals Antagonistic Potentials of Selected Soil Fungi Against Three Pathogenic Fungi of Tagetes Erecta L. and T. Patula L

2020 ◽  
Vol 29 (2) ◽  
pp. 147-153
Author(s):  
Mahfuza Aktar ◽  
Shamim Shamsi
Keyword(s):  
Radial Growth ◽  
Soil Fungi ◽  
Trichoderma Viride ◽  
Pathogenic Fungi ◽  
Complete Inhibition ◽  
Curvularia Lunata ◽  
Dual Culture ◽  
Volatile Metabolites ◽  
Culture Filtrates ◽  
Maximum Inhibition

Three antagonistic fungi were isolated from the field soil of blight infected Tagetes spp. by serial dilution method. The fungi were identified as Aspergillus flavus Link, A. niger van Tiegh. and Trichoderma viride Pers. Antagonistic potentiality of aforesaid fungi were evaluated against the pathogenic fungi of Tageteserecta L. and T. patula L. following "dual culture colony interaction" and volatile and nonvolatile metabolites. The pathogenic fungi were A. alternata, Aspergillus fumigatus and Curvularia lunata. In dual culture colony interaction, out of three soil fungi, T. viride showed the highest growth inhibition on A. alternata (71.03%), A. fumigatus (38.49%) and C. lunata (60.71%). The maximum inhibition of radial growth of A. alternata (74.55%) was observed with the culture filtrates of T. viride owing to volatile metabolites. The maximum inhibition of radial growth of A. fumigatus (37.43%) was observed with the culture filtrates of A. flavus owing to volatile metabolites. The complete inhibition of radial growth of C. lunata was observed with the culture filtrates of A. niger owing to volatile metabolites. The complete inhibition of radial growth of A. alternata was observed with nonvolatile metabolites of A. nigera and T. viride at all concentrations. The complete inhibition of radial growth of A. fumigatus was also observed with nonvolatile metabolites of A. niger at all concentrations. Aspergillus niger and T. viride may be exploited commercially as a biocontrol agent against blight pathogens of T. erecta and T. patula. Dhaka Univ. J. Biol. Sci. 29(2): 147-153, 2020 (July)

scholarly journals Pathogenic potentiality of fungi associated with the seeds of different cotton (Gossypium hirsutum L.) varieties in Bangladesh

2020 ◽  
Vol 29 (1) ◽  
pp. 107-115
Author(s):  
Amina Khatun ◽  
Shamim Shamsi ◽  
MA Bashar
Keyword(s):  
Seed Germination ◽  
Rhizoctonia Solani ◽  
Aspergillus Flavus ◽  
Colletotrichum Gloeosporioides ◽  
Pathogenic Fungi ◽  
Curvularia Lunata ◽  
Vigor Index ◽  
Aspergillus Sp ◽  
Fusarium Nivale

A total of 24 species of fungi, namely Aspergillus flavus Link, A. fumigatus Fresenius, A. niger (Type 1 and Type 2) Van Tiegh, A. ochraceus K. Wilhelm, A. nidulans Eidam, Aspergillus sp.1, Aspergillus sp.2, Aspergillus sp.3, Curvularia lunata (Wakker) Boedijn, Colletotrichum gloeosporioides Penz & Sacc, C. gossypii Southw., Chaetomium globosum Kunze., Fusarium nivale (Fr.) Sorauer, F. moniliforme J. Shelden, F. oxysporum Schlechtendal, F. fujikuroi Nirenberg, Mucor sp. P. Micheli ex L., Penicillium sp.1 and sp.2 Link, Rhizoctonia solani Khun., Rhizopus stolonifer (Ehrenb.) Vuill., Rhizomucor sp. Lucet & Costantin, Syncephalastrum racemosum Cohn and Trichoderma viride Pers. were found to be associated with the seeds of 14 varieties (CB 1- CB 14) of cotton. Out of these 24 fungal species, nine were found to be pathogenic to cotton. They were Aspergillus flavus, A. niger (Type 1), Aspergillus sp. 1, Colletotrichum gloeosporioides, Curvularia lunata, Fusarium nivale, F. moniliforme, Mucor sp. and Rhizoctonia solani. These pathogenic fungi had remarkable effect on seed germination, vigor index, root-shoot length and mortality of cotton seedlings. The germination percentage of control seeds was 88 but because of the presence of pathogenic fungi the rate showed considerable reduction in all the varieties and it varied from 20 to 82%. Among the nine fungal isolates Rhizoctonia solani showed maximum reduction in seed germination. Aspergillus flavus, Colletotrichum gloeosporioides and Fusarium moniliforme also caused near about 50% reduction in seed germination. Mortality percentage of control seedling were also less (6) whereas, in inoculated seeds it was higher and varied from 7 - 23%. Root- shoot ratio of control seedlings was high but less in inoculated seedlings. The vigor index of control plant was high (1548.8) but less in inoculated plants. The lowest vigor index was noticed for Rhizoctonia solani (202.0) and highest for Aspergillus sp. 1(1213.6). Results indicated that Curvularia lunata and Rhizoctonia solani showed a greater impact in reduction of cotton seed germination and vigor index. Dhaka Univ. J. Biol. Sci. 29(1): 107-115, 2020 (January)

scholarly journals Molecular characterization and vegetative growth of pathogenic seed-borne fungus, Curvularia lunata of tomato and its in vitro control measures

2022 ◽  
Vol 11 (2) ◽  
pp. 124-132
Author(s):  
MB Billah ◽  
MM Sikder ◽  
MRI Mallik ◽  
MK Hossain ◽  
N Alam
Keyword(s):  
Vegetative Growth ◽  
Mycelial Growth ◽  
Culture Media ◽  
Pathogenic Fungus ◽  
Molecular Techniques ◽  
Control Measures ◽  
Curvularia Lunata ◽  
Its Sequencing ◽  
Fungal Culture

Present studies were conducted to isolate and identify the seed-borne pathogenic fungus from the selected tomato variety through morphological and molecular techniques based on the sequencing of internal transcribed spacer (ITS) region of 18S rDNA. According to the colony and conidial features, the fungus was identified as Curvularia sp. The obtained ITS sequencing showed above 99% similarity with Curvularia lunata in the NCBI database. The sequence of the fungus was deposited in NCBI GenBank under the accession number: ITS, MH382879.1. Besides, the phylogenetic tree further confirmed the taxonomic position of the studied fungus. Growth characteristics of the fungus on nine different fungal culture media were evaluated, in which Honey peptone agar, Carrot agar, Potato sucrose agar, and Kauffman’s agar were found the most suitable. The maximum vegetative growth of the fungus was recorded at 30°C temperature and pH conditions. The bio-control potential of five different antagonists against the studied fungus was assessed, in which Trichoderma harzianum showed the better performance to restrict mycelial growth. Three ethanolic plant extracts were also evaluated, in which Lowsonia inermis L. exhibited above 60% mycelial growth inhibition of the fungus. Among three tested fungicides, Tilt 250 EC was found as an excellent fungicide to inhibit mycelial growth of C. lunata under in vitro conditions. Int. J. Agril. Res. Innov. Tech. 11(2): 124-132, Dec 2021

scholarly journals Using Commercial Compost as Control Measures against Cucumber Root-Rot Disease

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Kamel Kamal Sabet ◽  
Magdy Mohamed Saber ◽  
Mohamed Adel-Aziz El-Naggar ◽  
Nehal Samy El-Mougy ◽  
Hatem Mohamed El-Deeb ◽  
...  
Keyword(s):  
Root Rot ◽  
Control Measure ◽  
Disease Incidence ◽  
Pathogenic Fungi ◽  
Pythium Ultimum ◽  
Control Measures ◽  
Infested Soil ◽  
Fungal Isolates ◽  
Cucumber Root

Five commercial composts were evaluated to suppress the root-rot pathogens (Fusarium solani (Mart.) App. and Wr, Pythium ultimum Trow, Rhizoctonia solani Kuhn, and Sclerotium rolfsii Sacc.) of cucumber plants under in vitro and greenhouse conditions. In vitro tests showed that all tested unautoclaved and unfiltrated composts water extracts (CWEs) had inhibitor effect against pathogenic fungi, compared to autoclaved and filtrated ones. Also, the inhibitor effects of 40 bacteria and 15 fungi isolated from composts were tested against the mycelial growth of cucumber root-rot pathogens. Twenty two bacteria and twelve fungal isolates had antagonistic effect against root-rot pathogens. The antagonistic fungal isolates were identified as 6 isolates belong to the genus Aspergillus spp., 5 isolates belong to the genus Penicillium spp. and one isolate belong to the genus Chaetomium spp. Under greenhouse conditions, the obtained results in pot experiment using artificial infested soil with cucumber root-rot pathogens showed that the compost amended soil reduced the percentage of disease incidence, pathogenic fungi population, and improved the cucumber vegetative parameters as shoot length, root length, fresh weight, and dry weight. These results suggested that composts are consequently considered as control measure against cucumber root-rot pathogens.

scholarly journals Stimulatory Effects of Sublethal Doses of Dimethachlon on Sclerotinia sclerotiorum

Plant Disease ◽  
2014 ◽  
Vol 98 (10) ◽  
pp. 1364-1370 ◽  
Author(s):  
Feng Zhou ◽  
Hong-Jie Liang ◽  
Ya-Li Di ◽  
Hong You ◽  
Fu-Xing Zhu
Keyword(s):  
Oilseed Rape ◽  
Sclerotinia Sclerotiorum ◽  
Mycelial Growth ◽  
Plant Disease ◽  
Pathogenic Fungi ◽  
Potato Dextrose Agar ◽  
Detached Leaves ◽  
Sublethal Doses ◽  
Fungal Phytopathogens ◽  
Plant Disease Management

Growth and virulence stimulations of sublethal doses of fungicides on plant-pathogenic fungi and oomycetes have been reported and the stimulatory effects are potentially relevant to plant disease management. Sclerotinia sclerotiorum is one of the most devastating and economically important necrotrophic fungal phytopathogens, capable of infecting more than 400 species of plants worldwide. In order to study stimulatory effects of sublethal doses of fungicides on S. sclerotiorum, 55 dimethachlon-sensitive isolates and 3 dimethachlon-resistant isolates of S. sclerotiorum were assayed to determine effects of sublethal doses of dimethachlon on mycelial growth rate on potato dextrose agar (PDA) media and virulence on oilseed rape plants. Results showed that all 3 dimethachlon-resistant isolates and 13 of the 55 sensitive isolates exhibited stimulatory responses to sublethal doses of dimethachlon. Dimethachlon-resistant isolates grew significantly (P < 0.05) faster on PDA media amended with dimethachlon at 0.5 to 4 μg/ml than on fungicide-free PDA media. As for virulence on detached leaves of oilseed rape plants, lesion diameters of dimethachlon-resistant isolates after growth on PDA media amended with dimethachlon at 0.5 to 2 μg/ml were significantly larger (P < 0.05) than the control. The maximum stimulatory effects were 42.40 to 59.80%. In pot experiments, for both dimethachlon-sensitive and -resistant isolates, significant (P < 0.05) virulence stimulations were observed after spraying with dimethachlon at a concentration of 2 μg/ml. After growing on dimethachlon-amended PDA media, H2O2 sensitivity of S. sclerotiorum decreased significantly (P < 0.05) compared with the nonamended PDA control.

scholarly journals Evaluation of Bioagents and Biopesticides against Colletotrichum lindemuthianum and its Integrated Management in Common Bean

2010 ◽  
Vol 2 (3) ◽  
pp. 72-76 ◽  
Author(s):  
Bilal Ahmad PADDER ◽  
Prem Nath SHARMA ◽  
Renu KAPIL ◽  
Anju PATHANIA ◽  
Om Prakash SHARMA
Keyword(s):  
Mycelial Growth ◽  
Integrated Management ◽  
Trichoderma Viride ◽  
Significant Inhibition ◽  
Colletotrichum Lindemuthianum ◽  
Maximum Inhibition ◽  
Gliocladium Virens ◽  
Foliar Treatment

Three bioagents (Trichoderma viride, T. harzianum and Gliocladium virens) and five biopesticides (Achook, Neemgold, Wannis, Spictaf and Neemazal) were evaluated under in vitro and in vivo conditions against Colletotrichum lindemuthianum. All the three antagonistic fungi caused significant inhibition of mycelial growth, maximum being with T. viride (69.21%) followed by T. harzianum (64.20%). Among the biopesticides tested at four concentrations, Wanis applied @ 1000 ?l/ml caused maximum inhibition of 82.12 per cent followed by Spictaf (52.85%). T. viride and Wanis @ 1000 ?l/ml were most effective in reducing the seed borne infection. Integration of bioagents with Bavistin showed that disease can be effectively managed with seed dressing either with Bavistin or biopesticide followed by foliar treatment with fungicide or biopesticide.

scholarly journals The diversity of fungi colonizing necrotic inflorescence buds of rhododendron (Rhododendron L.)

2013 ◽  
Vol 66 (2) ◽  
pp. 79-84 ◽  
Author(s):  
Małgorzata Żołna ◽  
Barbara Kierpiec-Baran ◽  
Maria Kowalik
Keyword(s):  
Dominant Species ◽  
Trichoderma Viride ◽  
Ornamental Plants ◽  
Pathogenic Fungi ◽  
Lower Number ◽  
Botanical Species ◽  
Fusarium Poae ◽  
Identification Of Fungi ◽  
Improved Cultivars ◽  
Genetically Determined

<p>The infection of rhododendron (<em>Rhododendron </em>L.) inflorescence buds caused by pathogenic fungi induces its browning, withering, and dieback. The identification of fungi causing the infection of rhododendron inflorescence buds can be a reason for creating new improved cultivars with genetically determined resistance to pathogens. The investigations were carried out in 2010–2011 on the collection of ornamental plants of the Faculty of Horticulture, University of Agriculture in Kraków. The material comprised infected inflorescence buds collected from nine newly bred taxa and one botanical species of rhododendron. 596 colonies of fungi belonging to 31 species were isolated from infected rhododendron inflorescence buds. The dominant species were: <em>Pestalotiopsis sydowiana</em>, <em>Truncatella truncata</em>, <em>Alternaria alternata</em>, <em>Phialophora asteris,</em> and <em>Trichoderma viride</em>, which constituted almost 74% of the isolated fungi population.<em> Boeremia exigua </em>var<em>. exigua</em>,<em> Epicoccum nigrum</em>, <em>Fusarium poae</em>, <em>Mammaria echinobotryoides</em>, <em>Paraphoma chrysanthemicola</em>, <em>Phialophora cyclaminis</em>,<em> Phoma eupyrena</em>, <em>Talaromyces wortmannii</em>, <em>Umbelopsis isabellina</em>, and other fungi were isolated in a lower number.</p><p>The results of mycological analysis confirm the diversity of species colonizing necrotic inflorescence buds of rhododendron.</p>.

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