scholarly journals Determination of the serum antibody level of different age groups of broiler chicken against Infectious Bursal Disease (IBD)

2016 ◽  
Vol 4 (2) ◽  
pp. 22-27
Author(s):  
MI Hossain ◽  
BK Nath ◽  
MAM Prodhan
Keyword(s):  
Antibody Titer ◽  
Broiler Chickens ◽  
Serum Antibody ◽  
Age Groups ◽  
Infectious Bursal Disease ◽  
Broiler Chicks ◽  
Protective Level ◽  
Bursal Disease ◽  
The Mean

A study on Infectious Bursal Disease (IBD) was conducted on broiler chicks (N=200+70) of strains: Cobb-500 and Hubbard classic at Laxmipur district of Bangladesh. The blood sample were collected at day old chicks (1st day), pre-vaccinated flocks (11th day) and post-vaccinated flocks (26th day), to measure the antibody titer by indirect ELISA method. Maternal derived antibody (MDA) mean titer of day old chicks (N=40) were found 9621.65 ± 780.78 and 4232.60 ± 301.66 for Cobb-500 and Hubbard classic respectively. MDA mean titer was measured at day eleven chicken (N=60) before vaccination and found 1963.00 ± 143 and 984.16 ± 126.4037 respectively. Of these two titers; Cobb-500 strain was found to be protective (>1000) where Hubbard classic was below protective (<1000) titer level. The two flocks of Cobb-500 and Hubbard classic strains of broiler chickens were vaccinated with intermediate (Bursine-2) and intermediate plus (IBD Blen) vaccine respectively at day twelve and serum was harvested on day twenty six. The mean serum titers were found 131.30 ± 36.04 and 7413.54 ± 569.39 in Cobb-500 and Hubbard classic respectively, where mean titer level of Cobb-500 was below the minimum protective level but Hubbard classic strain was developed 7413.54, protective level. These results were indicating that the maternal derived antibody titer persists above 1000 level hindering the development of antibody titer in vaccinated flocks. However, persistence of MDA titer above 1000 may causes of vaccination failure of IBD vaccine.International Journal of Natural Sciences (2014), 4(2) 22-27

scholarly journals Effect of Dietary Combination of Methionine and Fish Oil on Cellular Immunity and Plasma Fatty Acids in Infectious Bursal Disease Challenged Chickens

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Elham Maroufyan ◽  
Azhar Kasim ◽  
Goh Yong Meng ◽  
Mahdi Ebrahimi ◽  
Loh Teck Chwen ◽  
...  
Keyword(s):  
Immune Response ◽  
Fish Oil ◽  
Broiler Chickens ◽  
Dietary Treatment ◽  
Blood Parameters ◽  
Infectious Bursal Disease ◽  
Broiler Chicks ◽  
Treatment Groups ◽  
Bursal Disease ◽  
Methionine Supplementation

This study was carried out to investigate the modulatory effects of dietary methionine and fish oil on immune response, plasma fatty acid profile, and blood parameters of infectious bursal disease (IBD) challenged broiler chickens. A total of 300 one-day-old male broiler chicks were assigned to one of six dietary treatment groups in a 3 × 2 factorial arrangement. There were three levels of fish oil (0, 2.5 and 5.5%), and two levels of methionine (NRC recommendation and twice NRC recommendation). The results showed that the birds fed with 5.5% fish oil had higher total protein, white blood cell count, and IL-2 concentration than those of other groups at 7 days after IBD challenge. Inclusion of fish oil in diet had no effect on IFN-γconcentration. However, supplementation of methionine twice the recommendation enhanced the serum IFN-γand globulin concentration. Neither of fish oil nor methionine supplementation affected the liver enzymes concentration. It can be suggested that a balance of moderate level of fish oil (2.5%) and methionine level (twice NRC recommendation) might enhance immune response in IBD challenged broiler chickens.

scholarly journals A Novel Adjuvant “Sublancin” Enhances Immune Response in Specific Pathogen-Free Broiler Chickens Inoculated with Newcastle Disease Vaccine

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Yangke Liu ◽  
Jiang Zhang ◽  
Shuai Wang ◽  
Yong Guo ◽  
Tao He ◽  
...  
Keyword(s):  
Antibody Titer ◽  
Newcastle Disease ◽  
Lymphocyte Proliferation ◽  
Broiler Chickens ◽  
B Lymphocyte ◽  
Serum Antibody ◽  
Broiler Chicks ◽  
Specific Pathogen Free ◽  
Blood Samples ◽  
Specific Pathogen

Sublancin is a glycosylated antimicrobial peptide produced by Bacillus subtilis 168 possessing antibacterial and immunomodulatory activities. This study was aimed at investigating the effects of sublancin on immune functions and serum antibody titer in specific pathogen-free (SPF) broiler chickens vaccinated with Newcastle disease (ND) vaccine. For this purpose, 3 experiments were performed. Experiment 1: SPF broiler chicks (14 days old) were randomly allotted to 1 of 7 groups including a blank control (BC), vaccine control (VC), and 5 (3-7) vaccinated and sublancin supplemented at 5, 15, 30, 45, and 60 mg activity/L of water, respectively. Vaccinated groups (2-7) were vaccinated with ND vaccine by intranasal and intraocular routes at the 14th day. On 7, 14, 21, and 28 days post vaccination (dpv), the blood samples were collected for the determination of serum hemagglutination inhibition (HI) antibody titer. Experiment 2: SPF broiler chicks were divided into 1 of 3 groups, i.e., blank control (BC), vaccine control (VC), and sublancin treatment (ST). On 7, 14, and 21 dpv, the blood samples were collected for measuring HI antibody titer by micromethod. Experiment 3: the design of this experiment was the same as that of experiment 2. On 7 and 21 dpv, pinocytosis of peritoneal macrophages, B lymphocyte proliferation assay, measurement of CD4+ and CD8+ T cells, and serum cytokine quantitation were carried out. It was noted that sublancin promoted B lymphocyte proliferation, increased the proportion of CD8+ T lymphocyte subpopulations, and enhanced the antibody titer in broiler chickens. In addition, it was also observed that sublancin has the potential to induce the secretion of IFN-γ, IL-10, and IL-4. In conclusion, these findings suggested that sublancin could promote both humoral and cellular immune responses and has the potential to be a promising vaccine adjuvant.

scholarly journals Dietary Grape (Vitis vinifera) Seed Powder and Zn–Gly Chelate Complex for Mitigating Heat Stress in Broiler Chickens: Growth Parameters, Malondialdehyde, Paraoxonase-1, and Antibody Titer

Agriculture ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1087
Author(s):  
Muhammad Israr ◽  
Naila Chand ◽  
Rifat Ullah Khan ◽  
Ibrahim A. Alhidary ◽  
Mutassim M. Abdelrahman ◽  
...  
Keyword(s):  
Heat Stress ◽  
Antibody Titer ◽  
Broiler Chickens ◽  
Growth Traits ◽  
Growth Parameters ◽  
Control Diet ◽  
Paraoxonase 1 ◽  
Control Treatment ◽  
Broiler Chicks ◽  
Feed Conversion

A total of 300 day old broiler chicks (Hubbard) were assigned to 30 floor pens (10 birds per pen) under cyclic heat stress. Three diets including a control, as well as two levels of grape seed powder (GSP) and zinc (OZ) at the rates of 2.5 g/kg GSP + 50 mg/kg OZ and 5 g/kg GSP + 50 mg/kg OZ, were supplied to the broilers for 35 days. According to the results, broiler feed intake improved (p < 0.05) in GSP + OZ groups from 3–5 weeks and on an overall basis compared to the control diet. Body weight increased (p < 0.05) in GSP-5 + OZ-50 during weeks 2–5 and on an overall basis. The findings indicated that feed conversion ratio (FCR) decreased (p < 0.05) during week 5 in broilers supplemented with GSP-5 + OZ-50. The antibody titer (HI) against Newcastle disease (ND) was higher (p < 0.05) in GSP + OZ groups compared to control treatment. The value of malondialdehyde (MDA) decreased (p < 0.05) under GSP + OZ diets compared to control. Moreover, paraoxonase (PON1) was higher (p < 0.05) in GSP + OZ groups compared to untreated broilers. In conclusion, GSP + OZ positively supported growth traits, reduced MDA, and augmented PON1 and HI titer against ND in broilers exposed to heat stress.

scholarly journals Studi Lapang: Penegakan Diagnosis Infectious Bursal Disease (IBD) Pada Ayam Broiler

2019 ◽  
Vol 3 (1) ◽  
pp. 25-30
Author(s):  
Aan Awaludin ◽  
Yudhi Ratna Nugraheni ◽  
Theo Mahiseta Syahniar ◽  
Dyah Laksito Rukmi ◽  
Agus Hadi Prayitno ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Care Management ◽  
Clinical Symptoms ◽  
Viral Disease ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Bursal Disease ◽  
Commercial Farms ◽  
The One ◽  
Organ Changes

Infectious Bursal Disease (IBD), also called Gumboro, was disease which attacked cells in the bursa of fabricius, causing interference with the chicken's immune system or immunosuppressive. IBD was the one of viral disease that often attacks chickens in the field. The aim of this study was to determine the diagnosis of IBD by through at clinical symptoms and necropsy that can still be relevant on in the field. The diagnosis of IBD correctly, cheaply, easily and quickly in the field is very important to optimize the health care management and evaluation program. The method used was by observing clinical symptoms of broiler chickens in commercial farms suspected of contracting IBD and observing post-death organs (necropsy). The object of necropsy was 5 samples of broiler chickens from the farm. Data was analyzed descriptively. The results of this study was the broiler chickens that infected with IBD could be diagnosed through clinical symptoms and post-death organ changes, so that the diagnosis for IBD cases in the field used the observation of clinical symptoms and necropsy are still relevant.

scholarly journals Q&A on the Paper of Kurukulasuriya et al. (2017) on IBD Vaccine Efficacy Against a Canadian Variant IBDV Strain in Broiler Chickens

2018 ◽  
Vol 3 (1) ◽  
Keyword(s):  
Antibody Titer ◽  
Vaccine Efficacy ◽  
Broiler Chickens ◽  
Live Vaccine ◽  
The Other ◽  
In Ovo ◽  
Challenge Strain ◽  
Vector Vaccine ◽  
The Mean ◽  
Modified Live Vaccine

Kurukulasuriya, et al. (2017) are reporting the efficacy of two IBD vaccines against an early (6 days post-hatch) challenge with a variant Canadian IBDV strain in broilers. A modified live vaccine(UNIVAX BD) administered by SQ route at 1 dayof-age delayed infection whereas an HVT-IBD vector vaccine (VAXXITEK HVT+IBD) administered in ovodid not protect. Furthermore, the authors suggested that the HVT-IBD vector induced immunosuppression responsible for an earlier IBDV challenge strain replication in the bursa. The data presented in the paper showed no evidence of VAXXITEK HVT+IBD vaccine take since the mean IBD ELISA antibody titer at D35 in the vaccinated/non-challenged group was not significantly different from that of the non-vaccinated group. It wasmuch lower than the expected one based on previous studies performed in the same conditions : in ovo vaccination of broilers [1,2]. Since there is no evidence of vaccine take, the other potential effects (immunosuppression and earlier IBDV replication in the bursa) observed in that group cannot be attributed to the vaccine. Since its launch in 2006 in Brazil, VAXXITEK HVT+IBD has been licensed in more than 75 countries and more than 80 billion birds have been vaccinated. VAXXITEK HVT+IBD is protecting against a wide variety of IBDV strains including the classical, the very virulent and different variant strains. To our knowledge, noabsence of efficacy nor bursa depletions have been so far officially reported as long as the vaccine has been administered properlyto healthy embryonated eggs or to healthy one-day-old chicks.

scholarly journals Immune Response Study to Live Infectious Bursal Disease Vaccines in Broiler Chickens

2017 ◽  
Vol 6 (12) ◽  
pp. 2655-2668 ◽  
Author(s):  
Pooja Kundu ◽  
G. Narang ◽  
Rajesh Chhabra ◽  
Sushma Kajal ◽  
N.K. Mahajan ◽  
...  
Keyword(s):  
Immune Response ◽  
Broiler Chickens ◽  
Infectious Bursal Disease ◽  
Bursal Disease
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