In Vitroseed Germination And Micropropagation Of Dendrobiumlindleyisteud. An Indigenous Orchid Of Bangladesh

2015 ◽  
Vol 41 (2) ◽  
pp. 163-171
Author(s):  
SK Bhadra ◽  
TK Bhowmik
Keyword(s):  
Plant Growth ◽  
Germination Rate ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Shoot Bud ◽  
Multiple Shoot Buds ◽  
Half Strength ◽  
Basal Media ◽  
Rapid Elongation ◽  
Benzyl Amino Purine

The seeds of Dendrobium lindleyi were germinated on 0.8% (w/v) agar solidified Murashige and Skoog (MS) as well as Phytamax (PM) basal media. The germination rate was found to be better on PM medium. The germinated seedlings underwent rapid elongation on Plant Growth Regulators (PGRs) supplemented media and the highest growth was recorded on MS with 3% (w/v) sucrose + 2.0 mg/1 6-Benzyl Amino Purine (BAP) + 0.1mg/1 Indole-3-Butyric Acid (IBA). The seed derived seedlings developed strong and stout roots on half strength MS medium with 1.5% sucrose. For micropropagation the pseudobulb segments were used as explant and the lower part of pseudobulb segment produced highest number of multiple shoot buds (9-10/segment) on agar solidified MS medium supplemented with 3% (w/v) sucrose + 2.5 mg/1BAP + 0.1% (w/v) Activated Charcoal. The highest rate of elongation and rooting was found from multiple shoot bud derived seedlings as well as same seed derived seedlings. Seed and multiple shoot bud derived rooted seedlings were finally transferred to outside natural environment by successive phases of acclimatization. Asiat. Soc. Bangladesh, Sci. 41(2): 163-171, December 2015

scholarly journals In vitro Embryo Morphogenesis and Micropropagation of Dendrobium aggregatum Roxb.

2014 ◽  
Vol 23 (2) ◽  
pp. 241-249 ◽  
Author(s):  
Mohammad Musharof Hossain
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Developmental Stages ◽  
Cell Mass ◽  
Multiple Shoot ◽  
Seedling Development ◽  
Ms Medium ◽  
Multiple Shoot Buds ◽  
Half Strength

In vitro embryo morphogenesis and micropropagation of Dendrobium aggregatum Roxb. were described. The gradual developmental stages of embryos to seedlings were traced out. Within two weeks of culture the cells of undifferented embryos underwent repeated aniclinal and periclinal division producing a compact, green parenchymatous cell mass called spherule that emerged out by rupturing the testa. The spherules subsequently differentiated into greenish protocorms were considered as typical seed germination. Germination occurred on both (MS and Phytamax (PM) medium but MS medium proved to be more efficient. The primary protocorms underwent profuse proliferation through production of secondary (2º) protocorms when transferred to different plant growth regulators (PGRs) supplemented MS; the medium fortified with 2.0 mg/l BAP and 1.0 mg/l NAA proved to be most effective for induction of 2º protocorms and seedling development. Multiple shoot buds (MSBs) were induced in pseudobulb segments of the in vitro grown seedlings when cultured on different PGRs supplemented media; and the maximum number of MSBs were obtained MS + 2.0 mg/l BAP + 0.5 mg/l picloram. The MSBs underwent elongation and then they rooted when they were transferred to half strength of MS + 0.5 mg/l IAA. The well rooted plantlets were finally transferred to outside natural environment with 80% survival. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17525 Plant Tissue Cult. & Biotech. 23(2): 241-249, 2013  (December)

scholarly journals An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita)

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501
Author(s):  
Sanjog T. Thul ◽  
Arun K. Kukreja
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Mentha X Piperita ◽  
Half Strength ◽  
Multiple Shoot Regeneration

A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint ( Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L-l zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L−1) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half - strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.

scholarly journals Mass Propagation of Feronia limonia L. through Tissue Culture

1970 ◽  
Vol 45 (1) ◽  
pp. 75-78 ◽  
Author(s):  
Shahina Islam ◽  
Mosfequa Zahan ◽  
Shahina Akter ◽  
Tanjina Akhtar Banu ◽  
Ahashan Habib ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Key Words ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Explants ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation

An efficient mass propagation method for Feronia limonia was developed from excised shoot tips and nodal explants of in vitro grown seedlings. Explants were cultured on MS medium with different conc. of NAA, Kn, IAA and BAP singly or in combinations. Highest number of micro shoots and better plant growth were obtained from these two explants on MS medium supplemented with 0.2 mg/l BAP alone. The regenerated shoots were successfully rooted on MS medium supplemented with 0.5 mg/l NAA. The in vitro raised plantlets were successfully established in soil following the formation of roots with 100% survivability under ex vitro condition. Key words: Feronia limonia; Mass propagation; Node; Shoot tips; Multiple shoot DOI: 10.3329/bjsir.v45i1.5186 Bangladesh J. Sci. Ind. Res. 45(1), 75-78, 2010

scholarly journals In vitro regeneration of two high-yielding eggplant (Solanum melongena L.) varieties of Bangladesh

1970 ◽  
pp. 08-12
Author(s):  
Sabina Yesmin, Mst Muslima Khatun, Tanzena Tanny ◽  
Anica Tasnim Protity ◽  
Md Salimullah ◽  
Iftekhar Alam
Keyword(s):  
In Vitro Regeneration ◽  
Solanum Melongena ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Healthy Plant ◽  
Ms Medium ◽  
Best Response ◽  
Half Strength ◽  
Multiple Shoot Regeneration

An in vitro regeneration protocol was developed for two high-yielding eggplant varieties (Solanum melongena L.) namely BARI begun-4 and BARI begun-6. Multiple shoots were regenerated from cotyledonary explants through organogenesis with growth regulators of different combinations and concentrations.  The best response towards multiple shoot regeneration was achieved from cotyledon explants on MS media complemented with 1 mg/l BAP + 0.2 mg/l IAA in both the two varieties of eggplant. Elongation of shoots was achieved on hormone free MS medium. Regenerated shoots of both the varieties produced   active in vitro root system on half strength of MS medium supplemented with 0.2 mg/l IBA.  The in vitro grown plantlets were acclimatized in soil, grew up to maturity, flowered, fruited and produced seeds as normal healthy plant like the control.

scholarly journals In vitro Plant Regeneration of Magnolia punduana: An Endemic and Threatened Plant Species

2017 ◽  
Vol 27 (2) ◽  
pp. 153-159 ◽  
Author(s):  
Rajib Borah ◽  
Suman Kumaria ◽  
Hiranjit Choudhury
Keyword(s):  
Plant Regeneration ◽  
Plant Growth ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
In Vitro Plant Regeneration ◽  
Threatened Plant Species ◽  
Callus Initiation ◽  
Half Strength ◽  
Basal Media

Shoots were induced from axillary and nodal buds of Magnolia punduna on MS supplemented with 0.1 μm of BAP. Out of five basal media tested (MS, ½ MS, ¼ MS, LS and WP), MS was found to be most effective for shoot and callus initiation. Different plant growth regulators (0.1 ‐ 1.0 μm) induced shoot formation in different proportions. The combination of 0.1 μm IBA and 0.5 μm BAP was found optimum for shoot elongation with minimal necrosis of the explants. Half strength of MS supplemented with 8.0 μm IBA was found suitable for rooting.Plant Tissue Cult. & Biotech. 27(2): 153-159, 2017 (December)

scholarly journals In vitro micropropagation of Rauvolfia serpentina (L.) Benth through induction of direct and indirect organogenesis

2013 ◽  
pp. 01-09
Author(s):  
SK Bhadra ◽  
TK Bhowmik ◽  
P Singh
Keyword(s):  
Callus Tissue ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Indirect Organogenesis ◽  
Rauvolfia Serpentina ◽  
Shoot Buds ◽  
Wide Range ◽  
Multiple Shoot Buds

Leaf and nodal segments of two months old field grown seedlings of Rauvolfia serpentina (L.) Benth were aseptically cultured on agar solidified MS medium supplemented with various combinations and concentrations of auxins (NAA, IAA, 2,4-D and picloram) and cytokinins (BAP and Kn). The nodal segments produced highest number of multiple shoot buds (5.85/explant) on MS medium supplemented with 2.0 mgl-1 BAP + 0.2 mgl-1 NAA or 2.0 mgl-1 BAP + 0.1 mgl-1 IAA. Whereas nodal segment produced callus tissue of different nature on MS medium supplemented with 1.5 mgl-1 BAP + 0.5 mgl-1 IAA+ 1.5 mgl-1 2,4-D; 3.0 mgl-1 BAP + 1.0 mgl-1 NAA + 1.5 mgl-1 Kn and 0.1 mgl-1 Pic + 1.0 mgl-1 Kn. The callus tissue of light green and nodular nature on further subculture in a wide range of plant growth regulators (PGRs) supplemented media, differentiated into multiple shoot buds that underwent rapid elongation on 2.0 mg/l BAP and 0.2 mg/l NAA supplemented media. The elongated shoot buds on further subculture in rooting media produced strong and stout roots. Half strength MS with 1.5% (w/v) sucrose was most effective for enhancing rooting. Finally those plantlets were acclimatized in field. Thus a protocol was established for rapid micropropagation of this medicinal plant through induction of direct and indirect organogenesis from nodal explant. DOI: http://dx.doi.org/10.3329/cujbs.v3i1.13401 The Chittagong Univ. J. B. Sci.,Vol. 3(1&2):01-09, 2008

scholarly journals In Vitro Propagation of Pumpkin and Ash Gourd through Nodal Segments

1970 ◽  
Vol 16 ◽  
pp. 67-71 ◽  
Author(s):  
ME Haque ◽  
MAR Sarkar ◽  
MA Mahmud ◽  
D Rezwana ◽  
B Sikdar
Keyword(s):  
Shoot Multiplication ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Shoot Bud ◽  
Benincasa Hispida ◽  
Ash Gourd

The present study was undertaken with a view to develop an efficient protocol for in vitro multiple shoot formation and subsequent root induction considering various cultural aspects using nodal segments of Cucurbita maxima and Benincasa hispida. The best hormone for shoot multiplication of pumpkin was BAP (2 mg/l), incase of ash-gourd BAP was 1.5 mg/l. For callus induction BAP+2,4-D was best combination for pumpkin and it was 2.0+0.1 mg/l when in ash gourd BAP+NAA was the best combination. IBA + ½MS medium were used for induction of shoot bud root. In both of pumpkin and ash gourd 1.5 mg/l IBA was found best for induction of roots. Key words: In vitro, MS medium, nodal segments, multiplication, rooting, pumpkin and ash gourd.   DOI:10.3329/jbs.v16i0.3743 J. bio-sci. 16: 67-71, 2008

scholarly journals Plantlet regeneration from nodal explants of Ocimum citriodorum Vis.

2013 ◽  
Vol 47 (4) ◽  
pp. 433-436
Author(s):  
Balasundaram Janarthanam ◽  
Sumathi Ethiraj
Keyword(s):  
Growth Response ◽  
Average Length ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Average Height ◽  
Ms Medium ◽  
Farm Yard Manure ◽  
Multiple Shoot Induction ◽  
Half Strength ◽  
Indole 3 Acetic Acid

A protocol for multiple shoot induction and plant regeneration from nodal explants of Ocimum citriodorum has been developed. Nodal explants inoculated on Murashige and Skoog (MS) medium supplemented with 1.0 mg/l Benzyl adenine (BAP) and 0.025 mg/l indole -3- acetic acid (IAA) showed better growth response (80%) and produced 15.2 ± 1.28 shoots per explant with an average length of 6.17 ± 0.29 cm after 35 days. Roots were induced after transfer to half strength MS medium supplemented with 0.5 mg/l Indole -3- butyric acid (IBA) produced 6.0 ± 1.0 roots with an average height of 4.9 ± 0.26 cm after 30 days. Plantlets with well developed root and shoot systems were successfully acclimated (80 %) and established in earthen pots containing mixture of soil, vermiculite and farm yard manure (1:1:1). Bangladesh J. Sci. Ind. Res. 47(4), 433-436, 2012 DOI: http://dx.doi.org/10.3329/bjsir.v47i4.8547

scholarly journals Development of an Embryo Rescue Protocol for Butterfly Weed

2020 ◽  
Vol 30 (1) ◽  
pp. 31-37
Author(s):  
Mary Lewis ◽  
Matthew Chappell ◽  
Donglin Zhang ◽  
Rebekah Maynard
Keyword(s):  
Germination Rate ◽  
Embryo Rescue ◽  
The United States ◽  
Commercial Production ◽  
Optimal Time ◽  
Propagation Time ◽  
Ms Medium ◽  
Native Plant ◽  
Half Strength ◽  
Soilless Substrate

We documented a successful embryo rescue (ER) protocol for butterfly weed (Asclepias tuberosa), a member of the milkweed family (Asclepiadaceae). Milkweed (Asclepias sp.) includes more than 100 species native to the United States, is an important pollinator plant, and has many commercially desirable traits. However, there is little commercial production outside of native plant nurseries because milkweed species are typically seed-grown and suffer from low seed set during pollination, late-term abortion of seed pods, and nonuniform germination. This project determined the optimal growing media (study one) and embryo maturity (study two) to recover mature seedlings from excised embryos and compared the results to those of traditional methods of seed germination (in soilless substrate). Study one investigated three different media: Murashige and Skoog (MS) medium at full strength and half strength and woody plant medium. MS medium at half strength was optimal for butterfly weed germination and maturation, with greater root and shoot lengths at the time of harvest. In study two, the effects of MS medium at half strength on embryo maturation 90, 60, and 30 days after pollination (DAP) were investigated. The optimal time to harvest embryos was 60 DAP; embryos at 30 DAP were capable of germination but not maturation. A mean germination rate of 97.4% was observed when using embryo rescue, but it was 72.3% with mature seed germinated in soilless substrate typical of commercial production. A similar increase in germination rates was observed for all embryo maturities when compared with seed germinated using soilless substrate. The protocol developed for this study should help to standardize production, reduce propagation time, and improve the commercial acceptance and profitability of milkweed.

scholarly journals In vitro propagation of banana

1970 ◽  
Vol 34 (2) ◽  
pp. 269-278
Author(s):  
M Rezaul Karim ◽  
MA Malek ◽  
Sajia Rahman ◽  
M Al-Amin ◽  
M Ruhul Amin
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Room Temperature ◽  
Ms Medium ◽  
Musa Sp ◽  
In Vitro Technique ◽  
Half Strength ◽  
The Mean ◽  
Basal Media

An in vitro technique for plant regeneration using meristem-derived plantlets of banana cv. BARI-l (Musa sp.) has been developed. Highest number of shoot regeneration was noticed on basal media supplemented with 7.5 mgL-1 BAP + 0.5 mgL-1 NAA at 30 days after inoculation (DAI). The mean number of shoots significantly reduced when the concentrations of BAP and NAA in the medium was high. Regenerated shoots were rooted on half strength MS medium containing 0.5 mgL-1 IAA + 0.5 mgL-1 IBA at 30 DAI. In vitro raised plantlets were transferred to poly bags containing ground soil and cowdung mixture (1:1) for acclimatization and hardening in room temperature (28-30°C) and the established plantlets are ready for planting in the field. Key Words: In vitro propagation; banana; Musa sp.DOI: 10.3329/bjar.v34i2.5799Bangladesh J. Agril. Res. 34(2): 269-278, June 2009

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