Histological study of the developing gonads of endangered freshwater spiny eel, Mastacembelus armatus during the reproductive cycle

The spiny eel, Mastacembelus armatus is one of the most common and economically important inland teleost species of Asia having high market and nutritive values. The demand for the fish is almost always exceeds its supply. Histological analysis of developmental stages of both oocytes and sperrnatocytes maturation were undertaken and dimensions of ovarian oocytes were observed to evaluate the annual reproductive condition of M. armatus during August 2012 to July 2013. Monthly sampling of adult individuals of M. armatus was done from haor region of Kishoreganj district, Bangladesh. Both the gonads were paired, elongated and of unequal in length. The colour of ovaries varied from reddish brown to light yellowish; whereas, testes varied from whitish to creamy or dull white. From the histological study, five stages of oocyte development (oogonia, chromatin nucleolar stage oocyte, perinucleolar stage oocytes, yolk vesicle oocyte, and yolk granule stage) were observed upon their changes in nucleus. Female M. armatus having immature stages of oocytes (early and late perinucleolar stage oocytes) were mostly available in the months of November and December. Yolk vesicle stage oocytes appeared during the months of January and February. Early yolk granule stage oocytes appeared during the months of March and April while late yolk granule stage oocytes were observed during the months of May and June. Oocytes and nuclei diameters were observed ranged from 121050 ?m and 10212 ?m, respectively until ovulation. In male gonads, four stages of spermatogenesis (spermatogonia, spermatocytes, spermatids, and spermatozoa) were distinguished during the study period where spermatids were prominent in January to March and spermatozoa in April to July. From this study, the peak gonadal development of M. armatus is found in June and thus, it is clearly indicated that the fish breed during May to June.J. Bangladesh Agril. Univ. 14(2): 261-269, December 2016

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On the reproduction of the Mediterranean keystone limpet Patella rustica: histological overview

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s0025315414000976 ◽

2014 ◽

Vol 94 (8) ◽

pp. 1651-1660 ◽

Cited By ~ 4

Author(s):

Ivana Prusina ◽

Daria Ezgeta-Balić ◽

Stijepo Ljubimir ◽

Tatjana Dobroslavić ◽

Branko Glamuzina

Keyword(s):

Reproductive Cycle ◽

Shell Length ◽

Histological Analysis ◽

Developmental Stages ◽

Gonad Development ◽

Gonad Index ◽

Keystone Species ◽

Males And Females ◽

Sexually Mature ◽

High Shore

The reproductive cycle of the high shore limpet Patella rustica is described based on histological analysis as the primary method of staging gonad development. Sex-ratios, shell length at sexual maturity, gonad developmental stages, mean gonad index and oocyte size were investigated. Males and females were found to differ in size distribution, with females becoming more prevalent from ~28 mm onwards. The estimated shell length at which 50% of males were sexually mature was 13.1 mm. Patella rustica has only one reproductive cycle per year with a spawning peak between November and December for both sexes, and gonad redevelopment from January. First data on the size–frequency analysis of oocytes for this species are also presented, concurring with the qualitative analysis of the gonad developmental stages. This study presents updated information on the reproductive cycle of this keystone species and provides the first account of the reproductive biology of P. rustica in the Adriatic Sea.

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Reproduction of the turban shell Turbo torquatus Gmelin 1791 (Mollusca : Gastropoda), in New South Wales, Australia

Marine and Freshwater Research ◽

10.1071/mf00066 ◽

2002 ◽

Vol 53 (1) ◽

pp. 85 ◽

Cited By ~ 8

Author(s):

D. W. Ward ◽

A. R. Davis

Keyword(s):

Reproductive Cycle ◽

Developmental Stages ◽

New South ◽

Gonadosomatic Index ◽

New South Wales ◽

Gonad Development ◽

Gonadal Development ◽

Small Scale ◽

Frequency Distributions ◽

South Wales

The Sydney turban shell Turbo torquatus is the focus of a small-scale commercial fishery in New South Wales. Effective management requires knowledge of the reproductive biology, yet this is lacking for NSW waters. The reproductive cycle was investigated at three localities on the southern New South Wales coast. Samples of T. torquatus were collected monthly at Wollongong, Ulladulla and Eden from February 1996 until August or December 1997. The reproductive cycle was investigated by three methods: monthly determination of a gonadosomatic index, estimation of oocyte size-frequency distributions and classification of female gonads into developmental stages following histological sectioning. Males and females within a population underwent synchronous gonad development and spawning. Spawning events were often protracted over a period of several months with females in various stages of gonadal development. Two spawning events occurred each year, with a spawning event in autumn–winter and another in spring–summer. These events were asynchronous among the three localities, and partial spawning appeared to be a common occurrence. Owing to variation in the timing of spawning between populations separated by a distance as small as 15 km, seasonal closures to protect spawning stocks are unlikely to be effective.

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Gonadal Development of Females of the White Shrimp Penaeus schmitti (Burkenroad, 1936) Caught in Southeastern Brazil

Journal of Marine Science and Engineering ◽

10.3390/jmse9060653 ◽

2021 ◽

Vol 9 (6) ◽

pp. 653

Author(s):

Roberta Cardozo P. Garcia ◽

Cecília Fernanda F. Craveiro ◽

Caroline V. Bernabé ◽

Maria Aparecida Silva ◽

Paulo Henrique R. Aride ◽

...

Keyword(s):

Native Species ◽

Histological Analysis ◽

Visual Analysis ◽

Developmental Stages ◽

Reproductive Potential ◽

Gonadal Development ◽

White Shrimp ◽

Southeastern Brazil ◽

Initial Development ◽

In Captivity

This study describes the reproductive cell development and ovarian developmental stages, and identifies the nongerminal components of white shrimp (Penaeus schmitti) caught off the southern coast of Espírito Santo, Brazil. P. schmitti specimens were collected monthly from March 2019 to February 2020, by artisanal fishing. All shrimps were analyzed macroscopically (n = 181) and females were randomly selected monthly (n = 154) for ovarian histological analysis. The ovaries were extracted for visual analysis and then submitted to histological analysis. Macroscopic analysis determined five stages of gonadal development from the color and turgidity of the fresh ovary. Histological observations allowed us to observe five stages of gonadal development: immature, initial development, advanced development, mature and spawning. This information are fundamental for understanding the reproductive aspects of P. schmitti, as well as other penaeid shrimps, in order to promote preservation of natural stocks and provide information to assist in the development of the reproductive potential of native species in captivity.

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Histological study on the gonadal development of Scatophagus argus

JOURNAL OF FISHERIES OF CHINA ◽

10.3724/sp.j.1231.2013.38442 ◽

2013 ◽

Vol 37 (5) ◽

pp. 696 ◽

Cited By ~ 4

Author(s):

Dan CUI ◽

Zhiwei LIU ◽

Nanxi LIU ◽

Yingying ZHANG ◽

Junbin ZHANG

Keyword(s):

Histological Study ◽

Gonadal Development ◽

Scatophagus Argus

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Gonadal development and the reproductive cycle of the geoduck clam (Panopea japonica) in the Sea of Japan

Aquaculture ◽

10.1016/j.aquaculture.2021.737606 ◽

2021 ◽

pp. 737606

Author(s):

Wen Zhao ◽

Ying Li ◽

Yuhang Wang ◽

Xinghong Luo ◽

Qidi Wu ◽

...

Keyword(s):

Sea Of Japan ◽

Reproductive Cycle ◽

Gonadal Development ◽

The Sea Of Japan

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A Histological Study of Ovarian Development in the Giant Red ShrimpAristaeomorpha foliacea(Crustacea: Decapoda: Aristeidae) from the Southern Tyrrhenian Sea (Western Mediterranean)

The Scientific World JOURNAL ◽

10.1100/2012/289608 ◽

2012 ◽

Vol 2012 ◽

pp. 1-9 ◽

Cited By ~ 6

Author(s):

Anna Perdichizzi ◽

Laura Pirrera ◽

Valeria Micale ◽

Ugo Muglia ◽

Paola Rinelli

Keyword(s):

Ovarian Development ◽

Developmental Stages ◽

Histological Study ◽

Western Mediterranean ◽

Ovary Development ◽

Tyrrhenian Sea ◽

Length Frequency ◽

Southern Tyrrhenian Sea ◽

Aristaeomorpha Foliacea ◽

Trawl Surveys

The reproductive features of the giant red shrimp,Aristaeomorpha foliacea, were investigated in the southern Tyrrhenian sea by experimental trawl sampling. The annual length-frequency distribution showed a multimodal trend in females, ranging between 16 and 67 mm carapace length (CL), and a unimodal trend in males (18–45 mm CL). Mature males occurred in different proportions all year round, while females displayed seasonal maturity (June—September), with a peak in July. Six oocyte developmental stages were identified, the most advanced of which (Pv, postvitellogenic) had never been described before in this species. Ovary development followed a group-synchronous pattern, with the yolked oocyte stock clearly separated from the reservoir of unyolked oocytes, suggesting thatA. foliaceais a total spawner, with determinate fecundity. Based upon histological findings, a revision of macroscopic maturity staging employed in Mediterranean bottom trawl surveys (MEDITS) is proposed.

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Histological analysis of the tongue, esophagus and crop of Southern Carcará (Polyborus plancus)

Research Society and Development ◽

10.33448/rsd-v10i2.12439 ◽

2021 ◽

Vol 10 (2) ◽

pp. e21410212439

Author(s):

Vanessa Sobue Franzo ◽

Leiny Paula de Oliveira ◽

Ana Carolina Pôssas Viana ◽

Adriano Tony Ramos ◽

Jéssica Fernanda Bertolino ◽

...

Keyword(s):

Digestive System ◽

Histological Analysis ◽

Squamous Epithelium ◽

Eating Habits ◽

Histological Study ◽

Pekin Ducks ◽

The Family ◽

Body Weights ◽

Stratified Squamous Epithelium ◽

Hematoxylin Eosin

The Southern Caracara is a bird of prey, of the family Falconidae, inhabits all the Brazilian territory, they are omnivorous birds adapted for hunting. The digestive system of the predators presents differences in their morphology due to their eating habits. Knowing the importance of the gastrointestinal system, this study aims to carry out a histological study of the tongue, esophagus and the crop of Polyborus plancus. For this purpose, eight avian of both sexes, with different body weights and ages were used, they were euthanized and the organs were collected, fixed, dehydrated and stained with hematoxylin-eosin. It was noted that the tongue has stratified squamous epithelium with mucous glands and with different degrees of keratinization. The esophagus and crop have four well developed muscle layers and mucous glands. Additionally, in the crop there is presence of an adventitious layer that consists of loose connective tissue. We conclude that the tongue, the esophagus and the crop of the Southern Caracara have similar histological organization of several birds like the Pekin Ducks, ostriches, domestic chicken and partridges.

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Stigmatic surface in the Vochysiaceae: reproductive and taxonomic implications

Acta Botanica Brasilica ◽

10.1590/s0102-33062009000300018 ◽

2009 ◽

Vol 23 (3) ◽

pp. 780-785 ◽

Cited By ~ 6

Author(s):

Renata Carmo-Oliveira ◽

Berta Lange de Morretes

Keyword(s):

Histological Analysis ◽

Developmental Stages ◽

Phylogenetic Reconstruction ◽

Breeding Systems ◽

Stigmatic Surface ◽

Central Brazil ◽

Fresh Material ◽

The Family ◽

Taxonomic Implications ◽

Trees And Shrubs

The Vochysiaceae are Neotropical trees and shrubs, common in the savanna areas in Central Brazil (Cerrados). The family has been traditionally divided into two tribes: Erismeae, with three genera, and Vochysieae, with five genera. We investigated the stigmatic surface of six Vochysiaceae species, belonging to four genera of Vochysieae: Vochysia, Salvertia, Callisthene and Qualea. Flowers and buds at different developmental stages were collected. Morphological features were observed on fresh material and stigmatic receptivity was inferred based on esterasic activity. Pistils were fixed and embedded in paraplast and sectioned on a rotary microtome; the sections were stained before histological analysis. Stigmas of open flowers were also observed by scanning electron microscopy. Stigmas of all species were wet and showed esterasic activity at pre-anthesis and anthesis stages. Stigmatic surface was continuous with transmitting tissue of glandular nature. Vochysia and Salvertia stigmatic surfaces were formed by multicelular uniseriate hairs, and species of the remaining genera showed papillate surface. The exudate over mature stigmas in all species flowed without rupture of stigmatic surface and pollen tubes grew down between hairs or papillae. Differences on the stigmatic surface agreed with a phylogenetic reconstruction that separated two clades and indicated that Vochysieae is not monophyletic. Stigmatic features could not be associated with pollination and breeding systems.

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Comprehensive analysis of miRNA-mRNA/lncRNA during gonadal development of triploid female rainbow trout (Oncorhynchus mykiss)

10.21203/rs.3.rs-31357/v2 ◽

2020 ◽

Author(s):

Tianqing Huang ◽

Wei Gu ◽

Enhui Liu ◽

Xiulan Shi ◽

Bingqian Wang ◽

...

Keyword(s):

Rainbow Trout ◽

High Throughput Sequencing ◽

Developmental Stages ◽

Expression Profiles ◽

Noncoding Rnas ◽

Gonadal Development ◽

Differentially Expressed ◽

Rainbow Trout Oncorhynchus Mykiss ◽

Oocyte Meiosis ◽

Non Coding Rna

Abstract Background: Chromosomal ploidy manipulation is one of the means to create excellent germplasm. Triploid fish could provide an ideal sterile model for the mechanism research of abnormality in meiosis. The complete understanding of the coding and noncoding RNAs regulating sterility caused by meiosis abnormality is still not well understood.Results: By high-throughput sequencing, we compared the expression profiles of gonadal mRNA, long non-coding RNA (lncRNA), and microRNA (miRNA) at different developmental stages [65 days post fertilisation (dpf), 180 dpf, and 600 dpf] between the diploid (XX) and triploid (XXX) female rainbow trout. A majority of differentially expressed (DE) RNAs were identified, and 22 DE mRNAs related to oocyte meiosis and homologous recombination were characterized. The predicted miRNA-mRNA/lncRNA networks of 3 developmental stages were constructed based on the target pairs of DE lncRNA-miRNA and DE mRNA-miRNA. According to the networks, meiosis-related gene of ccne1 was targeted by dre-miR-15a-5p_R+1, and 6 targeted DE lncRNAs were identified. Also, RT-qPCR was performed to validate the credibility of the network.Conclusions: This study explored the potential interplay between coding and noncoding RNAs during the gonadal development of polyploid fish. It provides full insights into polyploidy-associated effects on fertility of fish. These differentially expressed coding and noncoding RNAs provide a novel resource for studying genome diversity of polyploid induction.

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