scholarly journals In vitro protein digestibility of different feed ingredients in Thai koi (Anabas testudineus)

1970 ◽  
Vol 7 (1) ◽  
pp. 205-210 ◽  
Author(s):  
H Ali ◽  
MM Haque ◽  
MMR Chowdhury ◽  
MI Shariful
Keyword(s):  
Fish Meal ◽  
Protein Digestibility ◽  
Crude Enzyme ◽  
In Vitro Digestibility ◽  
Enzyme Extract ◽  
Bone Meal ◽  
Anabas Testudineus ◽  
Crude Enzyme Extract ◽  
Feed Ingredients

The study was carried out to determine relative protein digestibility (RPD) of different feed ingredients for Thai koi (Anabas. testudineus; n=22) using in vitro digestibility technique. Gut crude enzyme extracted from the experimental species was used to assay RPD using pH drop method. The RPD of fish meal (FM), meat & bone meal (M&B), shrimp meal (SM), soybean meal (SM), mustard oilcake (MOC) and rice polish (RP) were 78.08%, 72.82%, 20.65%, 76.08%, 67.39% and 35.86%, respectively when the respective ingredients were hydrolyzed by the gut crude enzyme extract of A. testudineus and caesin was used as the standard. The highest relative protein digestibility was found in fish meal (78.08 %) and the lowest was found in shrimp meal (35.65 %). The determined RPD of different feed ingredients can be used as the base information for the feed preparation of A. testudineus. Keywords: Protein digestibility; Thai koi (A. testudineus); In vitro digestibility DOI: 10.3329/jbau.v7i1.4985 J. Bangladesh Agril. Univ. 7(1): 205-210, 2009

scholarly journals In vivo and in vitro protein digestibility of formulated feeds for Artemesia longinaris (Crustacea, Penaeidae)

2009 ◽  
Vol 52 (6) ◽  
pp. 1379-1386 ◽  
Author(s):  
Analía Verónica Fernández Gimenez ◽  
Ana Cristina Díaz ◽  
Susana María Velurtas ◽  
Jorge Lino Fenucci
Keyword(s):  
Fish Meal ◽  
Protein Digestibility ◽  
Apparent Digestibility ◽  
Enzyme Extract ◽  
In Vitro Protein Digestibility ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Vitro Protein

This study was undertaken to determine the in vivo crude protein apparent digestibility in the prawn Artemesia longinaris, using feeds with 0.25% of chromic oxide and animal (fish meal, meat and bone meal and squid protein concentrate) and plant (soybean meal) ingredients. Three replicate groups of prawn were fed and the feces were collected. The rate of protein hydrolysis was measured in vitro using midgut gland enzyme extract from the prawns fed the respective feeds and was compared with those found with enzyme extract of wild prawn. The in vivo apparent digestibility coefficients showed significant differences among the feeds (P<0.05). Fish meal feed presented the highest digestibility (92%); intermediate digestibility (83%) was found for meat and bone meal feed, and the less digestible feed (63%) was that containing soybean meal and squid proteins concentrate. No significant differences in the in vitro protein digestibility were found among the experimental feeds. The results indicated the limitation of in vitro enzyme assays and that it should be complemented by in vivo studies.

scholarly journals Combined Effects of Calcium Addition and Thermal Processing on the Texture and In Vitro Digestibility of Starch and Protein of Black Beans (Phaseolus vulgaris)

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1368
Author(s):  
Marbie Alpos ◽  
Sze Ying Leong ◽  
Indrawati Oey
Keyword(s):  
Thermal Processing ◽  
Profile Analysis ◽  
Protein Digestibility ◽  
In Vitro Digestibility ◽  
Texture Profile ◽  
Black Beans ◽  
Calcium Addition ◽  
The Impact ◽  
Cacl2 Solution

Legumes are typically soaked overnight to reduce antinutrients and then cooked prior to consumption. However, thermal processing can cause over-softening of legumes. This study aimed to determine the effect of calcium addition (0, 100, 300, and 500 ppm in the form of calcium chloride, CaCl2), starting from the overnight soaking step, in reducing the loss of firmness of black beans during thermal processing for up to 2 h. The impact of calcium addition on the in vitro starch and protein digestibility of cooked beans was also assessed. Two strategies of calcium addition were employed in this study: (Strategy 1/S1) beans were soaked and then cooked in the same CaCl2 solution, or (Strategy 2/S2) cooked in a freshly prepared CaCl2 solution after the calcium-containing soaking medium was discarded. Despite the texture degradation of black beans brought about by increasing the cooking time, texture profile analysis (TPA) revealed that their hardness, cohesiveness, springiness, chewiness, and resilience improved significantly (p < 0.05) with increasing calcium concentration. Interestingly, beans cooked for 2 h with 300 ppm CaCl2 shared similar hardness with beans cooked for 1 h without calcium addition. Starch and protein digestibility of calcium-treated beans generally improved with prolonged cooking. However, calcium-treated beans cooked for 1 h under S2 achieved a reduced texture loss and a lower starch digestibility than those beans treated in S1. A lower starch digestion could be desired as this reflects a slow rise in blood glucose levels. Findings from this result also showed that treating black beans with high level of CaCl2 (i.e., 500 ppm) was not necessary, otherwise this would limit protein digestibility of cooked black beans.

scholarly journals Kinetic Analysis of Crude Enzyme Extract Produced Via Solid State Fermentation of Banana Pseudo Stem Waste

2021 ◽  
Author(s):  
Mira Chares Subash ◽  
Muthiah Perumalsamy
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Crude Enzyme ◽  
Enzyme Extract ◽  
Waste Biomass ◽  
Textile Processing ◽  
Banana Fiber ◽  
Crude Enzyme Extract ◽  
Banana Fibers ◽  
Pectinase Enzyme

Abstract Banana Pseudo stem waste after each harvest contributes about 70–80 Milli Tons Per hector. The banana pseudo stem will be thrown as waste biomass after each harvest as it is unstable for the upcoming harvest. The biggest challenge in banana cultivation is the utilization of biomass of banana pseusostem waste into valuable products. In this study, Xylano-pectinase enzyme extract was produced from banana pseudo stem waste under solid-state fermentation by Enterobacter cloacae PMC04. The highest pectinase and xylanase activities obtained using banana pseudo stem as carbon source were 124.62 U/ml and 173.81 U/ml respectively. Thermodynamics stated that range 40-50oC were considered to be the optimal temperature for xylano-pectinase enzyme production and subsequent degumming of banana fibers. The crude enzyme extract were then used in the degumming of banana fibers for textile application. Textile processing of banana fiber necessitates the removal of hemicellulose substance which can be achieved by crude xylano-pectinase enzyme. It was found that crude xylano-pectinase was efficient in the removal of hemicellulose substance from the fibers. Results obtained from this study demonstrate that the proposed bioprocess could be successfully applied for the degumming of banana fibers sustainably.

Mycological Survey of Chicken Feed and Some Feed Ingredients in Turkey

1988 ◽  
Vol 51 (10) ◽  
pp. 807-810 ◽  
Author(s):  
DILEK HEPERKAN ◽  
ÍHSAN ALPERDEN
Keyword(s):  
Fish Meal ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Mixed Feed ◽  
Feed Ingredients ◽  
Penicillium Aurantiogriseum ◽  
Sunflower Cake ◽  
Feed Samples ◽  
Mold Count ◽  
Meat Bone Meal

Level of mold contamination and mycoflora were determined for 144 mixed feed and feed ingredients, including corn, sunflower cake, soja cake, meat and bone meal, and fish meal. Four samples were found to be free of mold. Among the feed samples examined, the mold count has been found to be low (102 to 103 colonies/g) for fish meal, high (104 to 105 colonies/g) for meat-bone meal and sunflower cake, and extremely high (more than 105 colonies/g) for soja cake, corn and mixed feed. The predominant flora in the feed samples consisted of Penicillium, Aspergillus, Fusarium, Mucor and Eurotium, respectively. The most frequently encountered species was found to be Penicillium aurantiogriseum, followed by Aspergillus flavus.

Parathion Degradation by Xanthomonas sp. and Its Crude Enzyme Extract in Clay Suspensions

1996 ◽  
Vol 25 (6) ◽  
pp. 1248-1255 ◽  
Author(s):  
Segula Masaphy ◽  
Tzion Fahima ◽  
Dan Levanon ◽  
Yigal Henis ◽  
Uri Mingelgrin
Keyword(s):  
Crude Enzyme ◽  
Enzyme Extract ◽  
Crude Enzyme Extract ◽  
Clay Suspensions

scholarly journals In vitro solubility of meat and bone meal protein with different pepsin concentrations

2000 ◽  
Vol 30 (3) ◽  
pp. 489-492 ◽  
Author(s):  
Cláudio Bellaver ◽  
Dirceu Luís Zanotto ◽  
Antônio Lourenço Guidoni ◽  
Claudete Hara Klein
Keyword(s):  
Crude Protein ◽  
Protein Digestibility ◽  
Enzyme Concentration ◽  
Asymptotic Model ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Meal Protein ◽  
Protein Origin

In vitro protein digestibility of protein sources has been correlated with in vivo digestibility values. However, factors like protein origin, enzyme used and its concentration, pH and processing have been related with the significance of the correlation between the estimates. To address only the enzyme concentration factor, this paper had the objective of testing pepsin at 0.2, 0.02, 0.002 and 0.0002% using the standard AOAC (1995) procedure. Two meat and bone meals (MBM) with low and high crude protein (CP) content were used to determine the coefficient of solubility of CP in pepsin and HCl (CSCPPEPH). Centrifugation was used to establish the nitrogen (N) in the soluble phase, instead of filtration and analysis of N in the residue. The variance analysis and a non-linear asymptotic model were adjusted. The CSCPPEPH under different pepsin concentrations for the two MBM showed higher solubility discrimination with low pepsin concentration. The level of 0.0002% pepsin is better to predict the CP soluble in MBM. This finding implies the assumption that 0.2% pepsin found in the AOAC is not correct for the purpose of determining the range of solubility in high and low CP content in MBM.

scholarly journals In vitro techniques for the assessment of the nutritive value of feed grains for pigs: a review

1999 ◽  
Vol 50 (5) ◽  
pp. 871 ◽  
Author(s):  
Paul J. Moughan
Keyword(s):  
Nutritive Value ◽  
Protein Digestibility ◽  
In Vitro Assays ◽  
In Vitro Digestibility ◽  
In Vitro Techniques ◽  
Ileal Digestibility ◽  
Plant Fibre ◽  
Gross Energy

The philosophy inherent in developing in vitro digestibility assays for dietary energy and protein is reviewed and an historical account is given of the development of such assays for the pig. General principles to be considered in the development of in vitro digestibility assays are discussed, as are limitations of the in vitro approach. The importance of choosing the most appropriate in vivo measures of digestibility for the evaluation of in vitro assays is stressed. For protein sources that do not contain anti-nutritional factors or plant fibre, ‘true’ ileal digestibility should be the in vivo baseline, while plant proteins should be tested against ‘real’ ileal digestibility. There is a dearth of adequately conducted validation studies for in vitro digestibility assays. It appears that the 3-step (pepsin, pancreatin, Viscozyme) closed in vitro system to allow prediction of organic matter and gross energy digestibility in the pig has particular promise for practical feed evaluation. Similarly based protein digestibility assays may require further development before they can be applied with confidence.

scholarly journals The effect of cooking on in vitro digestibility of selected legumes

2009 ◽  
Vol 57 (5) ◽  
pp. 13-18
Author(s):  
Ranjani Amarakoon ◽  
Stanislav Kráčmar ◽  
Ignác Hoza ◽  
Pavel Budinský
Keyword(s):  
Glycine Max ◽  
Pisum Sativum ◽  
Dry Matter ◽  
Protein Digestibility ◽  
Cooking Time ◽  
Cooking Methods ◽  
In Vitro Digestibility ◽  
Pressure Cooking ◽  
Dry Matter Digestibility

The aim of this study was to investigate the effect of various cooking methods on nutritional qua­li­ty by evaluating in vitro digestibility of some selected legumes (two cultivars Pisum sativum and Glycine max). Samples were soaked in 0.2% NaHCO3 for 6 hours and then cooked by normal (20, 25, 30 and 35 min) pressure (8, 10, 12 and 14 min) and microwave (8, 10, 12 and 14 min) cooking. In vitro protein and dry matter digestibility were investigated. Pressure cooking and microwave cooking are recommended after soaking with the cooking time between 8–14 mins for P. sativum (Xantos and Svit) and G. max. Based on in vitro protein digestibility of all the cooking treatments, pressure cooking is the most effective.

scholarly journals Crude protein, fibre and phytic acid in vitro digestibility of selected legume and buckwheat samples

2013 ◽  
Vol 61 (1) ◽  
pp. 227-232 ◽  
Author(s):  
Petra Vojtíšková ◽  
Stanislav Kráčmar
Keyword(s):  
Phytic Acid ◽  
Crude Protein ◽  
Dry Matter ◽  
Protein Digestibility ◽  
The Other ◽  
In Vitro Digestibility ◽  
Common Beans ◽  
Commission Regulation ◽  
Phytate Content

The aim of this study was to determine crude protein, fibre and phytic acid in vitro digestibility of selected legumes and buckwheat products. All analyses except the phytic acid contents were performed in the line with the Commission Regulation (EC) No. 152/2009. A modified version of Holt’s Method was used for phytic acid (phytate) determination. None of all samples contained more than 11% of moisture. Soybeans are rich in crude protein; they contain nearly 40% of this compound. The content of crude protein in buckwheat flours was about 14%. The highest amount of phytate was found in common beans and soybeans-about 2 g/100 g of dry matter. On the other hand, the lowest phytate content was observed in buckwheat pasta (< 1 g/100 g). The quantification of phytate in F. esculentum groats was 1.9 g per 100 g of dry matter. In vitro digestibility was determined using an incubator Daisy and pepsin enzymes and the combination of pepsin and pancreatin. The highest coefficient of crude protein digestibility was discovered to be in peels and wholemeal flour. The greatest fibre digestibility coefficients were obtained for peels, which contain about 65% of fibre in their dry matter. When pepsin was used, a higher phytic acid digestibility coefficient for G. max, Ph. vulgaris, peels, flour, groats and broken groats was observed; while when the combination of pepsin and pancreatin was used, higher phytic acid digestibility coefficients for peas, lentil and wholemeal flour were observed.

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