scholarly journals Crude protein, fibre and phytic acid in vitro digestibility of selected legume and buckwheat samples

Author(s):  
Petra Vojtíšková ◽  
Stanislav Kráčmar

The aim of this study was to determine crude protein, fibre and phytic acid in vitro digestibility of selected legumes and buckwheat products. All analyses except the phytic acid contents were performed in the line with the Commission Regulation (EC) No. 152/2009. A modified version of Holt’s Method was used for phytic acid (phytate) determination. None of all samples contained more than 11% of moisture. Soybeans are rich in crude protein; they contain nearly 40% of this compound. The content of crude protein in buckwheat flours was about 14%. The highest amount of phytate was found in common beans and soybeans-about 2 g/100 g of dry matter. On the other hand, the lowest phytate content was observed in buckwheat pasta (< 1 g/100 g). The quantification of phytate in F. esculentum groats was 1.9 g per 100 g of dry matter. In vitro digestibility was determined using an incubator Daisy and pepsin enzymes and the combination of pepsin and pancreatin. The highest coefficient of crude protein digestibility was discovered to be in peels and wholemeal flour. The greatest fibre digestibility coefficients were obtained for peels, which contain about 65% of fibre in their dry matter. When pepsin was used, a higher phytic acid digestibility coefficient for G. max, Ph. vulgaris, peels, flour, groats and broken groats was observed; while when the combination of pepsin and pancreatin was used, higher phytic acid digestibility coefficients for peas, lentil and wholemeal flour were observed.

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 918 ◽  
Author(s):  
Francesco Serrapica ◽  
Felicia Masucci ◽  
Emiliano Raffrenato ◽  
Maura Sannino ◽  
Alessandro Vastolo ◽  
...  

Fifteen oilseed cakes from sunflower, pomegranate, cardoon, tobacco and hemp were characterized with regard to chemical composition, Cornell Net Carbohydrate and Protein System (CNCPS) fractionation, in vitro digestibility of dry matter, neutral detergent fiber, and crude protein. All the cakes presented low moisture, rather variable ether extract contents and medium to high levels of crude protein and neutral detergent fiber. The cakes significantly differed in terms of CNCPS partitioning and in vitro digestibility. Tobacco and hemp cakes presented high contents of slow degradable fractions of crude protein and carbohydrate joined to good post-ruminal protein digestibility. Cardoon cakes presented the highest rumen protein degradability. Based on crude protein content and intestinal digestibility of rumen undegraded protein, cakes of tobacco and hemp showed the better potential as alternative protein supplements for ruminants, while pomegranate appears to be the least suitable for ruminant feeding.


Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1273
Author(s):  
Rongrong Li ◽  
Mingli Zheng ◽  
Di Jiang ◽  
Pengjiao Tian ◽  
Menghu Zheng ◽  
...  

To develop an alternative high-protein forage resource to alleviate ruminant feed shortages, we investigated the effects of replacing alfalfa (Medicago sativa L.) with different ratios of paper mulberry (Broussonetia papyrifera L., RY) on fermentation quality, protein degradation, and in vitro digestibility of total mixed ration (TMR) silage. The TMR were made with alfalfa and RY mixtures (36.0%), maize meal (35.0%), oat grass (10.0%), soybean meal (7.5%), brewers’ grain (5.0%), wheat bran (5.0%), premix (1.0%), and salt (0.5%) on a dry matter basis, respectively. The alfalfa and RY mixtures were made in the following ratios of dry matter: 36:0 (RY0), 27:9 (RY9), 18:18 (RY18), 9:27 (RY27), and 0:36 (RY36). After ensiling for 7, 14, 28, and 56 days, fermentation quality, protein degradation, and microbial counts were examined, and chemical composition and in vitro digestibility were analyzed after 56 days of ensiling. All TMR silages, irrespective of the substitution level of RY, were well preserved with low pH and ammonia nitrogen content, high lactic acid content, and undetectable butyric acid. After ensiling, the condensed tannin content for RY18 silages was higher than the control, but non-protein nitrogen, peptide nitrogen, and free amino acid nitrogen contents was lower, while the fraction B1 (buffer-soluble protein) was not different among all the silages. Dry matter and crude protein digestibility for RY27 and RY36 silages was lower than the control, but there was no difference between control and RY18 silages. This study suggested that ensiling RY with alfalfa inhibited true protein degradation, but decreased in vitro dry matter and crude protein digestibility of TMR silages, and that 18:18 is the optimal ratio.


1966 ◽  
Vol 66 (3) ◽  
pp. 351-357 ◽  
Author(s):  
W. Ellis Davies ◽  
G. ap Griffith ◽  
A. Ellington

The primary growth of eight varieties of three species–white clover (3), red clover (4) and lucerne (1)–was sampled at fortnightly intervals and the percentage dry matter, in vitro digestibility, crude protein, water soluble carbohydrates, P, Ca, K, Na and Mg were determined.Differences between species were nearly always significant and the general order of merit was white clover, red clover and lucerne. The exceptions were for dry-matter percentage where this order was reversed, and red clover had the lowest Na and highest Mg content.


1980 ◽  
Vol 60 (3) ◽  
pp. 807-811 ◽  
Author(s):  
R. S. FULKERSON

Midas marrowstem kale (Brassica oleracea L.) was grown in different row width associations with United 106 corn (Zea maize L.) in two studies and ensiled in different moisture blends with corn stover in another. Highest dry matter yields were obtained where a single row of kale was grown at 30 cm to the side of a corn row. This combination also provided the lowest moisture content feed and the highest in vitro digestibility and crude protein content. Changing the corn row width had no significant effect upon yield, plant height, in vitro digestibility, kale leaf or corn ear content. Blending kale with corn stover to provide a silage of about 70% moisture increased the digestibility and protein content of the feed and provided a silage that kept well in storage.


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Alberto Niccolai ◽  
Manuel Venturi ◽  
Viola Galli ◽  
Niccolò Pini ◽  
Liliana Rodolfi ◽  
...  

AbstractThe aim of this work was to evaluate the influence of Arthrospira platensis F&M-C256 (spirulina) incorporation on the nutritional and functional properties of “crostini”, a leavened bakery product largely consumed in Italy and Europe. Sourdough was used as leavening and fermentation agent and three concentrations of A. platensis F&M-C256 were tested: 2%, 6% and 10% (w/w). Despite a lower volume increase compared to the control, the A. platensis F&M-C256 “crostini” doughs reached a technological appropriate volume after fermentation. At the end of fermentation, no significant differences in microorganisms concentrations were observed. A. platensis F&M-C256 “crostini” showed higher protein content compared to the control. Considering the European Commission Regulation on nutritional claims, “crostini” incorporated with 6% and 10% biomass can be claimed to be a “source of protein”. Six and ten percent A. platensis “crostini” also presented significantly higher antioxidant capacity and phenolics. A significantly lower value of in vitro dry matter and protein digestibility between A. platensis F&M-C256 “crostini” and the control was found. The overall acceptability decreased with increasing A. platensis F&M-C256 addition. The combination of spirulina biomass addition and the sourdough technology led to the development of a novel microalgae-based bakery product with nutritional and functional features.


2018 ◽  
Vol 41 (1) ◽  
pp. 39492 ◽  
Author(s):  
Rafael Henrique de Tonissi e Buschinelli de Goes ◽  
Luiz Henrique Xavier da Silva ◽  
Tatiana García Díaz ◽  
Antonio Ferriani Branco ◽  
Ana Lúcia Teodoro ◽  
...  

 The objective of this study was to evaluate the effect of the inclusion of sunflower cake replacing soybean meal in beef cattle diets on the in vitro digestibility of dry matter (IVDDM), organic matter (IVDOM), crude protein (IVDCP) and the ruminal fermentation kinetics and parameters. The experiment was analyzed according to a completely randomized design. The treatments consisted of four levels of sunflower cake, 0, 200, 400, 600 g kg-1, replacing soybean meal in the concentrate of beef cattle diets. The coefficients of IVDDM, IVDOM and IVDCP presented a quadratic effect with the addition of sunflower cake. The soluble fraction (fraction B) degradation rate and total gas production decreased linearly with the inclusion of sunflower cake. Values of pH in ruminal fluid were higher for levels 0, 200 and 600 g kg-1 sunflower cake. Sunflower cake can replace soybean meal by up to 280 g kg-1 in the concentrate of beef cattle diets, improving the in vitro digestibility of dry matter organic, matter and crude protein. Levels above 400 g kg-1 reduce ruminal digestion rate, digestibility and release of final fermentation products. 


1969 ◽  
Vol 49 (3) ◽  
pp. 351-359 ◽  
Author(s):  
Alastair McLean ◽  
S. Freyman ◽  
J. E. Miltimore ◽  
D. M. Bowden

Seasonal changes in the quality of pinegrass (Calamagrostis rubescens) on native forest range in southern British Columbia included declines in crude protein content and in vitro digestibility of dry matter and increases in content of lignin, acid-detergent fiber, and ash. The decline in average daily gains of beef heifers was more rapid than changes in quality of the pinegrass over the grazing period from June to October.Pinegrass contained adequate nutrients for the rapid growth of yearlings or for maintaining weanling calves until August 1, whereupon protein and phosphorus supplementation were required. Nutrient requirements of pregnant cows and replacement heifers were adequately met until September 1.Copper and zinc were inadequate throughout the season. Calcium, iron, and manganese contents, however, were satisfactory. Silica was high and must be considered a potential problem in pinegrass utilization.Digestible dry matter gave a high positive correlation with crude protein and phosphorus and a high negative correlation with lignin, acid-detergent fiber, and total ash.Crude fiber, crude fat, and total soluble carbohydrate contents of pinegrass did not closely follow changes in either animal gains or in vitro digestible dry matter.Crude protein, phosphorus, and acid-detergent fiber appear to give a good indication of animal yields on pinegrass and could be used to evaluate pinegrass quality where animal data are not available.


Author(s):  
Ranjani Amarakoon ◽  
Stanislav Kráčmar ◽  
Ignác Hoza ◽  
Pavel Budinský

The aim of this study was to investigate the effect of various cooking methods on nutritional qua­li­ty by evaluating in vitro digestibility of some selected legumes (two cultivars Pisum sativum and Glycine max). Samples were soaked in 0.2% NaHCO3 for 6 hours and then cooked by normal (20, 25, 30 and 35 min) pressure (8, 10, 12 and 14 min) and microwave (8, 10, 12 and 14 min) cooking. In vitro protein and dry matter digestibility were investigated. Pressure cooking and microwave cooking are recommended after soaking with the cooking time between 8–14 mins for P. sativum (Xantos and Svit) and G. max. Based on in vitro protein digestibility of all the cooking treatments, pressure cooking is the most effective.


2021 ◽  
Vol 22 (1) ◽  
pp. 68-75
Author(s):  
A. A. TAIWO ◽  
E. A. ADEBOWALE ◽  
A. O. AKINSOYINU ◽  
J. F. D. GREENHALGH

Feedblocks, poultry manure diet, gliricidia: leucaena mixture (1:1 W/W) and maize offal diet were compared as sources of protein supplements for WAD sheep fed a basal diet of elephant grass hay (Pennisetum purpureum), through a 12-week performance and digestibility study. Dry matter and organic matter intakes (DMI, OMI) of the sheep were higher (P0.05) on the gliricidia: leucaena supplement than on the other diets. DMI on poultry manure and maize offal diets were not significant (P0.05). Organic matter intake (OMI) of the animals followed the same trend as DMI. Sheep fed legume supplements excelled in intakes of crude protein (CP), acid detergent fibre (ADF) and digestibilities of dry matter (DM), organic matter (OM) and neutral detergent fibre (NDF) (P>0.05). Intakes of CP and ADF by sheep on the other supplements were not significantly different (P>0.05). Sheep fed on feedblock supplement consumed the least amount. (P0.05) of NDF. Variations were observed in DM,OM, and NDF digestibilities by sheep on maize offal, poultry manure and legumes supplements. Crude protein digestibility in the legumes and feedblocks supplements was higher (P0.05) than with the other two supplements. All the sheep used gained weights but those on maize offa' diet recorded higher (P0.05). weight gain (86.57g) than those on browse leaves (59.40g), feedblocks (47.80g) and poultry manure (40.44g). Rumen pH was lower (P<0.05) on the gliricidia: leucaena supplement than on other diets. Total VFA recorded for sheep on maize offal diet and legumes supplements was higher (P<0.05) than for the other two supplements. Blood urea nitrogen of the supplements were not significantly different (P>0.05) 


2020 ◽  
Vol 17 (1) ◽  
pp. 45
Author(s):  
Tri Astuti

This research aimed to study effect of Corn cob fermentation using Panerochaeta chrysosporium with adding the different sources of carbohydrat on the digestibility of dry matter, organic matter, and crude protein by in-vitro methode. A Completely Randomized Design  with 4 treatments and  4 replications. The treatments  were : P0 = fermented corncobs + without carbohydrt, P1 = fermented corncobs + 10% rice bran; P2 = fermented corncobs + tapioca flour 10%; P3 = corncob fermented + molasses 10%. The results of this study shown a very significant affected  (P <0.01) on the digestible of dry matter and organic matter but no affected (P> 0.05) on crude protein digestibility. The best results  digestibility of dry matter (66.98%) on the  P3 treatment adding  molasses, and  the highest digestibility of organic matter (67.49%) on the P3 treatment too, but the highest digestibility of  crude protein (51.37%)  in P1 treatment (adding by rice bran)


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