Immunogenicity of BAU-Bivalent Salmonella and Fowl Cholera Vaccines in Shuvra Chickens

The study was performed to investigate the immunogenicity of Salmonella and Fowl cholera vaccine prepared at BAU using Shuvra strain of chickens administered with BAU Salmonella bivalent and BAU Fowl cholera vaccines prepared by Livestock and Poultry Vaccine Research and Production Centre, (LPVRPC). A total of 60 apparently healthy BLRI strains Shuvra were used in this study. The birds were divided into three groups, consisting of 20 birds in each group. Shuvra- A group birds were vaccinated with BAU Salmonella bivalent vaccine, Shuvra-B group birds with BAU Fowl cholera vaccines and Shuvra-C group birds were used as unvaccinated control. The birds, except the ones in group C, were vaccinated intramuscularly (0.5ml) at 8 weeks of age followed by a booster dose at 12 weeks of age. Sera samples were collected on day 56, 85, 105 days and after challenge at 140 days age of birds. Secondary (booster) vaccination (P< 0.05) exhibited a significant level of antibody response. The antibody titre of these birds slowly descended till four weeks of challenge test. It was observed that a dependable level of immunogenicity of BAU Salmonella Bivalent and BAU Fowl cholera vaccine was observed when administered under this schedule followed in Shuvra strain of chickens. DOI: http://dx.doi.org/10.3329/mh.v1i2.14089 Microbes and Health, 2012 1(2): 50-53

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Immune response of fowl cholera vaccine produced at Bangladesh Agricultural University in farm level

Research in Agriculture Livestock and Fisheries ◽

10.3329/ralf.v2i1.23035 ◽

2015 ◽

Vol 2 (1) ◽

pp. 103-107

Author(s):

Md Abdus Sattar Bag ◽

Md Mansurul Amin ◽

Md Bahanur Rahman ◽

Yasir Ahammad Arafat ◽

Md Salim ◽

...

Keyword(s):

Booster Vaccination ◽

Primary Vaccination ◽

Control Group ◽

Cholera Vaccine ◽

Vaccine Research ◽

Farm Level ◽

Fowl Cholera ◽

Unvaccinated Control ◽

The Mean ◽

Poultry Vaccine

The work was carried out to determine the immunogenicity of fowl cholera vaccine (FCV) produced by Livestock and Poultry Vaccine Research and Production Centre (LPVRPC) at Bangladesh Agricultural University (BAU). A total five hundred of sevenweek old Hy-sex chickens (both white and brown) were vaccinated @ 0.5 ml of 2.93×108 CFU through subcutaneous route in each selected groups such as A1, A2 and A3; and B1, B2 and B3. Booster dose was provided at 13 weeks of age in group A3 and B3. Group C was kept as unvaccinated/control. Postvaccination sera were collected at different time schedule from all the groups of birds and antibody against fowl cholera were determined by Passive haemagglutination (PHA) test. At 4 weeks of primary vaccination (11 weeks aged birds) the mean PHA titres of sera were 96.00±34.21 and 96.00±34.21in group A1 and B1 respectively. On the other hand mean PHA titres at 5-weeks following vaccination (12 weeks aged birds) were 88.00±33.12 and 96.00±34.21in group A2 and B2, respectively. After 4 weeks of booster vaccination the mean PHA titres were 104.00±33.12in A3 and 104.00±33.12 in B3 group. The mean PHA titres in chickens of unvaccinated control group C was <4±0.00.Fowl cholera vaccine prepared at LPVRPC induced a good level of immunity at the farm level and it was also demonstrated that booster (secondary) vaccination is essential to develop protective level of immunity.Res. Agric., Livest. Fish.2(1): 103-107, April 2015

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Comparative efficacy of BAU-fowl cholera and DLS-fowl cholera vaccines in indigenous chicken

Research in Agriculture Livestock and Fisheries ◽

10.3329/ralf.v5i2.38108 ◽

2018 ◽

Vol 5 (2) ◽

pp. 193-199

Author(s):

Chamak Nahar Shampa ◽

Suma Akter ◽

Sukumar Saha ◽

Md Hadiuzzaman ◽

Azhar Ul Alam ◽

...

Keyword(s):

Pasteurella Multocida ◽

Research Work ◽

Booster Vaccination ◽

Indigenous Chicken ◽

Passive Hemagglutination ◽

Fowl Cholera ◽

Unvaccinated Control ◽

Group A ◽

The Mean ◽

Group B

The present study was conducted to determine the immune response induced in indigenous chicken produced against BAU-FC and DLS-FC vaccines with their efficacy study against Pasteurella multocida. A total of forty (40) chickens were selected and divided into Group A (15), Group B (15) and Group C (10). Group A and B were vaccinated with BAU-FCV and DLS-FCV, respectively at the dose rate of 0.5 ml through SC at six weeks of age followed by boostering at 10 weeks of age while Group C was kept as unvaccinated control. Sera samples were collected after primary and booster vaccination and antibody titre was determined by Passive hemagglutination (PHA) test. The mean PHA titres recorded at 4 weeks after primary vaccination was 51.20 ± 7.84 in birds of group A and 38.40 ± 6.40 in birds of Group B. After booster vaccination, mean PHA titer was found 140.80 ± 31.35 at 16 weeks of age in case of BAU-FC vaccinated group and 115.20 ± 12.80 in case of DLS-FC vaccinated group. The mean PHA titer was 204.80 ± 31.35 and 179.20 ± 31.35 at 19 weeks of age in birds of BAU-FC and DLS-FC vaccinated group, respectively. Birds of all groups were challenged with virulent P. multocida at 17 weeks of age. It was observed that vaccinated chickens showed maximal resistance (100%) following challenge with virulent whereas unvaccinated control birds failed to resist the challenge infection. It can be assumed from the findings of present research work that both BAU-FCV and DLS-FCV are able to protect indigenous chicken from the outbreak of avian pasteurellosis and BAU-FV vaccine showed relatively higher immuno-protective titre than that of DLS-FC vaccine.Res. Agric., Livest. Fish.5(2): 193-199, August 2018

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Comparative immunogenicity of fowl cholera vaccine in Jinding ducks

Bangladesh Veterinarian ◽

10.3329/bvet.v30i2.18253 ◽

2014 ◽

Vol 30 (2) ◽

pp. 41-45 ◽

Cited By ~ 1

Author(s):

S Sultana ◽

S Saha ◽

MM Amin

Keyword(s):

Pasteurella Multocida ◽

Booster Vaccination ◽

Primary Vaccination ◽

Challenge Infection ◽

Fowl Cholera ◽

Significant Difference ◽

Unvaccinated Control ◽

Antibody Titres ◽

Group A ◽

Group B

This study compared the immunogenicity of alum-precipitated formalin-killed fowl cholera vaccines (BAU-FCV and LRI-FCV) in Jinding ducks. The ducks were divided into three groups (A = 14, B = 14, C = 12). Group A was inoculated with BAU- FCV 0.5 mL and group B with LRI- FCV 1.0 mL intramuscularly (im) at the age of six weeks and group C served as unvaccinated control. Booster vaccination was administered similarly at 11 weeks of age in groups A and B. Challenge infection was given to all birds two weeks after booster vaccination. Passive Haemagglutination Assay (PHA) antibody titres in group A were 59.4 ± 4.6 21 days after primary vaccination, 137.1 ± 21.8 15 days after booster vaccination, 100.6 ± 12.9 21 days after booster vaccination, and 256.0 ± 48.4 15 days after challenge. In group B, titres were 50.3 ± 6.5, 118.9 ± 9.1, 91.4 ± 12.9, 237.7 ± 51.7, respectively, whereas titres in group C remained at ?4.0 ± 0.0. The antibody titres were insignificant when compared between pre-vaccination and 21 days after primary vaccination in both vaccinated groups (A and B). PHA antibody titres of groups A were significantly (P < 0.0001) increased at 15 days after booster and in case of group B the antibody titres were insignificant. At 15 days after challenge the antibody titres were highly significant in both groups (A and B). There was no significant difference between the two vaccinated groups. Following challenge infection with virulent Pasteurella multocida 88.9% of birds vaccinated with BAU-FCV, and 77.8% of birds vaccinated with LRI-FCV survived, while all unvaccinated birds died. Both vaccines were safe and effective. DOI: http://dx.doi.org/10.3329/bvet.v30i2.18253 Bangl. vet. 2013. Vol. 30, No. 2, 41-45

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Investigation on the Efficacy of a Killed Salmonella pullorum Vaccine

Microbes and Health ◽

10.3329/mh.v1i1.13707 ◽

2013 ◽

Vol 1 (1) ◽

pp. 14-18 ◽

Cited By ~ 1

Author(s):

Mst Shamima Akter ◽

Sukumar Saha ◽

Md Ariful Islam ◽

Md Golzar Hossain

Keyword(s):

Serum Antibody ◽

Booster Vaccination ◽

Antibody Responses ◽

Vaccine Research ◽

Lethal Challenge ◽

Whole Cells ◽

Salmonella Pullorum ◽

Antibody Titers ◽

Leukocyte Counts ◽

Poultry Vaccine

This study was carried out to determine the efficacy of a formalin-killed alum-precipitated Salmonella pullorum vaccine prepared by the Livestock and Poultry Vaccine Research and Production Centre (LPVRPC), BAU, Mymensingh, Bangladesh. Immunization with this vaccine induced serum antibody titers that peaked in 2-week following both primary and booster vaccination (P<0.05), and started to decline following 4-week of both vaccinations. Both primary and booster vaccination induced detectable antibody responses that were able to react with whole cells S. pullorum as determined by passive haemagglutination test (PHA). Both vaccinated chicken and mice showed maximal resistance following challenge with a virulent isolate of S. pullorum (P<0.01). In differential leukocyte counts in mice, a significant increase of lymphocytes was observed after primary immunization (P<0.01). Sera from vaccinated chickens conferred superior protection over naive chickens from lethal challenge with S. pullorum (P<0.01). Finally, this result indicates that S. pullorum vaccine is effective for controlling pullorum disease and antibody is crucial for protection.DOI: http://dx.doi.org/10.3329/mh.v1i1.13707 Microbes and Health Vol.1(1) June 2012 pp.14-18

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Comparative efficacy of avinew (VG/GA strain) and BCRDV (F strain) vaccines against Newcastle disease in broiler chickens

Bangladesh Journal of Veterinary Medicine ◽

10.3329/bjvm.v5i1.1304 ◽

1970 ◽

pp. 19-23

Author(s):

MS Mahmud ◽

MT Hossain ◽

P Monoura ◽

MM Amin

Keyword(s):

Newcastle Disease ◽

Challenge Test ◽

Maternal Antibody ◽

Case Group ◽

Broiler Chicks ◽

Comparative Efficacy ◽

Unvaccinated Control ◽

Group A ◽

Group B ◽

Sera Samples

A study was conducted for the detection of persistence of maternal antibody as well as the comparative efficacy of Avinew (VG/GA Strain) and BCRDV (F Strain) vaccines against Newcastle disease during the period from January to April 2006 using 100 day-old broiler chicks divided into three groups namely A, B and C. Birds of groups A and B consisting each of 40 were primarily vaccinated intraocularly (IO) with Avinew and BCRDV respectively at the age of day three (3) and secondarily with the same vaccines, as the cases were, on day 20. Sera samples were obtained from 10 randomly selected birds on each occasion of day 1, 10, 17, 27, 30, 34 and 44 of age of birds. Birds of group C were maintained as unvaccinated control for the determination of existence of maternal antibody parallel to the day of vaccination and challenge. It was observed that following primary vaccination in case of group A, the HI titres with a mean Â± SD on day 10 and 17 were 121.6 Â± 19.2 and 60.8 Â± 9.60 respectively as against at mean titres Â± SD of 57.6 Â± 12.8 and 30.4 Â± 4.80 in case group B respectively. On the other hand, subsequent to secondary vaccination, sera samples obtained from group A on day 27 had a mean Â± SD of HI titres 128 Â± 0 and in case of group B, the performance of Avinew in respect of such titre was 57.6 Â± 12.8 on day 27. Thus it was indicated that performance of Aninew in respect of elucidation of HI antibody was comparatively better than that of BCRDV. In case of unvaccinated control birds of group C, the mean of existence of maternal antibody with Â± SD were 512 Â± 0, 54.4 Â± 14.66, 24 Â± 8, 12.8 Â± 8.54, â¤ 4, â¤ 4 and â¤ 4 at the age of day 1, 10, 17, 27, 30, 34 and 44 respectively. One half of vaccinated birds of groups A and B were subjected to challenge test with a virulent isolate of NDV on each of two occasion of day 30 and 44 of age of birds where it was observed that 100% of both the groups of A and B were refractory to each test whereas 95% and 85% of the remaining half of birds of groups A and B resisted the challenge exposure. It was found that maternal antibody against NDV in chicks persisted to a minimal until the age of day 27 and none at day 30 or 34. The analysis of HI titres by Student's t-test revealed that Avinew vaccinated group maintained significantly higher HI titres following primary and secondary vaccination as well as during first challenge than that of BCRDV vaccinated group. Key words: Avinew, BCRDV, efficacy, vaccine, broiler chicks Â DOI = 10.3329/bjvm.v5i1.1304 Bangl. J. Vet. Med. (2007). 5 (1 & 2): 19-23

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HUMORAL IMMUNE RESPONSE TO FOWL CHOLERA VACCINE IN DIFFERENT BREEDS OF COMMERCIAL BIRDS

Bangladesh Journal of Veterinary Medicine ◽

10.3329/bjvm.v9i2.13453 ◽

2013 ◽

Vol 9 (2) ◽

pp. 127-131

Author(s):

MS Parvin ◽

MP Siddique ◽

MT Islam

Keyword(s):

Immune Response ◽

Rhode Island ◽

Antibody Titre ◽

Humoral Immune Response ◽

Serum Antibody ◽

Booster Vaccination ◽

Cholera Vaccine ◽

Fowl Cholera ◽

Humoral Immune ◽

Antibody Titres

Fowl cholera is a highly contagious and economically important disease of poultry worldwide. Control of fowl cholera depends mainly on vaccination throughout the world including Bangladesh. Therefore, the objective of the study was to determine the antibody titre following vaccination with fowl cholera vaccine in different breeds of commercial birds including Aseel and its F1 crosses. The study was conducted at Bangladesh Agricultural University Poultry Farm during the period from March to December 2011. A total of 37 birds of four types of breeds (Synthetic - 10, White Rock - 10, Aseel - 7 and Aseel×Rhode Island Red - 10) of both sex and 17 weeks old were used in this trial. Primary and booster vaccination were done in all the birds of four groups with fowl cholera vaccine (BAU-FCV) @ 0.5 ml/bird IM at 20 weeks and 26 weeks of age, respectively. Blood samples were collected at different occasions of vaccination. The immune responses (serum antibody titre) were determined by using passive haemagglutination assay (PHA). All the four groups of vaccinated birds induced significantly higher humoral immune response after primary and booster vaccination. However, no significant differences were observed in antibody titres between breeds on different occasions of vaccination. Of the four groups, antibody titres were slightly higher in breeds of Aseel×RIR and White Rock birds than other two breeds. It appears from the study that breed variation has no significant effect on immune response to fowl cholera vaccine.DOI: http://dx.doi.org/10.3329/bjvm.v9i2.13453

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Efficacy of Formalin Killed Fowl Cholera Vaccine in Experimentally Immunized Fayoumi Chickens

Bangladesh Journal of Veterinary Medicine ◽

10.3329/bjvm.v2i1.1929 ◽

1970 ◽

Vol 2 (1) ◽

pp. 23-25

Author(s):

MK Rahman ◽

MB Rahman ◽

MNA Siddiky ◽

MA Kafi ◽

MA Islam ◽

...

Keyword(s):

Pasteurella Multocida ◽

Subcutaneous Route ◽

Cholera Vaccine ◽

Passive Haemagglutination ◽

Fowl Cholera ◽

Virulent Isolate ◽

Unvaccinated Control ◽

Serotype 1 ◽

Antibody Titres ◽

Subcutaneous Inoculation

Efficacy of experimentally prepared formalin killed fowl cholera vaccine in Fayoumi chickens via different routes of vaccination was determined during the period from April 2002 to March 2003. Pasteurella multocida (PM-38) serotype 1 (X-73) was employed for vaccine preparation and antibody titres of the chicken sera were determined by passive haemagglutination (PHA) test. Vaccination was done either intramuscularly or subcutaneously. Each of the experimental chickens was challenged with a virulent isolate of P. multocida @ 3.8 x 108 CFU / ml per bird intramuscularly. The 100% vaccinated chickens protected against virulent P. multocida infection but all (100%) unvaccinated control birds died within 72 hours of challenge. Intramuscular (both primary and booster) route of vaccinations was found superior and more effective than subcutaneous route of inoculation. The higher PHA antibody titre was recorded with intramuscularly (222.86 Â± 25.60) than subcutaneously (111.43 Â± 12.80) vaccinated groups of birds. The result revealed the fact that intramuscular route followed by subcutaneous inoculation could be done for immunization against fowl cholera in chickens. Key words: Efficacy; fowl cholera vaccine; formalin killed; Fayoumi chicken doi: 10.3329/bjvm.v2i1.1929 Bangl. J. Vet. Med. (2004). 2 (1) : 23-25

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INVESTIGATION ON THE EFFICACY OF SALMONELLA GALLINARUM KILLED VACCINE

Bangladesh Journal of Veterinary Medicine ◽

10.3329/bjvm.v10i1-2.15645 ◽

2013 ◽

Vol 10 (1-2) ◽

pp. 41-49

Author(s):

R. Khatun ◽

M. A. Parvez ◽

M. G. Hossain ◽

S. Saha

Keyword(s):

Leukocyte Count ◽

Booster Vaccination ◽

Control Group ◽

Overall Survival Rate ◽

Vaccine Research ◽

Differential Leukocyte Count ◽

Passive Protection ◽

Salmonella Gallinarum ◽

Virulent Isolate ◽

Poultry Vaccine

The study was undertaken to investigate the efficacy of Salmonella gallinarum vaccine prepared at the Livestock and Poultry Vaccine Research and Production Centre (LPVRPC), Department of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh. The vaccine induced immune response both in chicken and mice are determined by PHA antibody titre and protection test. The highest PHA antibody titer was at 15 days after booster vaccination in both chickens and mice. The chickens and mice of vaccinated groups conferred 100% protection following challenge infection with the virulent isolate of Salmonella gallinarum given at 2 weeks after final immunization through i/m route (p <0.01). Differential leukocyte count (DLC) was performed in vaccinated mice and it was revealed that 11% increase in lymphocyte count in vaccinated group compared to control group (p <0.01). Finally, passive protection test in chickens that the protective value in terms of overall survival rate was 100% (p <0.001). These results clearly demonstrated that the Salmonella gallinarum vaccine of LPVRPC induced satisfactory level of antibody titre.DOI: http://dx.doi.org/10.3329/bjvm.v10i1-2.15645

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Comparative efficacy of BAU-Fowl cholera and DLS-Fowl cholera vaccines in indigenous ducks

10.33109/bjvmjd1801 ◽

2019 ◽

Author(s):

S. Akter ◽

C. N. Shampa ◽

M. A. Islam ◽

A. U. Alam ◽

M. Hadiuzzaman ◽

...

Keyword(s):

Pasteurella Multocida ◽

Booster Vaccination ◽

Significant Loss ◽

Challenge Infection ◽

Fowl Cholera ◽

Antibody Titres ◽

Group A ◽

The Mean ◽

Group B ◽

Cholera Vaccines

Background: Duck cholera is an acute, fatal, septicemic disease of domestic ducks which is responsible for significant loss in duck population. The present study was conducted to compare the immunogenicity of two formalin killed fowl cholera vaccines (BAU-FCV and DLS-FCV) in indigenous ducks. Methods: The experimental ducks were divided into three groups (A=15, B=15 and C =10 ducks) of which birds of Group A and Group B were inoculated with 0.5 ml of BAU-FCV and DLS-FCV, respectively through subcutaneous route at the age of 10 weeks whereas ducks of group C were kept as unvaccinated control. Booster vaccination was done with same dose and route at 14 weeks of age. Challenge infection was conducted after 2 weeks of booster vaccination. Results: The mean PHA antibody titres on 15 days post vaccination (DPPV), 28 DPPV, 15 days postsecondary vaccination (DPSV), 28 DPSV and 15 days post challenge were 25.60 ± 3.92, 51.20 ± 7.84, 89.60 ± 15.68, 166.40 ± 38.40 and 204.80 ± 31.35, respectively in ducks of Group A whereas, the mean antibody titres in ducks of Group B were 25.60 ± 3.92, 44.80 ± 7.84, 64.00 ± 7.53,102.40 ± 15.68 and 179.20 ± 31.35 at 15 DPPV, 28 DPPV, 15 DPSV, 28 DPSV and 15 days after challenge, respectively. In this investigation, slightly higher immune responses were observed in ducks of Group A vaccinated with BAU-FCV compare to ducks of Group B vaccinated with DLS-FCV. Birds of both vaccinated groups conferred 100% protection against challenge infection with virulent Pasteurella multocida whereas, 100% mortality was observed in control ducks after challenge. Conclusion: Both vaccines were found to be safe and effective for the vaccination of indigenous ducks against duck cholera.

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