Morphological and Molecular Confirmation of Parvatrema duboisi Metacercariae in the Manila Clam Ruditapes philippinarum from Gochang-gun, Korea

Gymnophallid metacercariae found in the Manila clam Ruditapes philippinarum (‘Banjirak’ in Korean) from Gochang-gun, Jeollabuk-do, Korea were morphologically and molecularly confirmed to be Parvatrema duboisi (Dollfus, 1923) Bartoli, 1974. The metacercariae were morphologically characterized by having a large oral sucker, small ventral sucker, genital pore some distance anterior to the ventral sucker, no ventral pit, and 1 compact or slightly lobed vitellarium, which were all compatible with P. duboisi. Some of the metacercariae were experimentally fed to mice, and adult flukes were recovered at day 7 post-infection. The morphology of the adult flukes was basically the same as that of the metacercariae except for the presence of uterine eggs; the uterus was filled with up to 40 eggs. The nucleotide sequences (1,193 bp) from ITS regions (ITS1, 5.8S rDNA, and ITS2) of the metacercariae showed 99.7% identity with P. duboisi and 75.7% identity with Gymnophalloides seoi deposited in GenBank. These results confirmed the presence of P. duboisi metacercariae in the Manila clam R. philippinarum in an estuary region of Gochang-gun, Korea.

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Parvatrema duboisi (Digenea: Gymnophallidae) Life Cycle Stages in Manila Clams, Ruditapes philippinarum, from Aphae-do (Island), Shinan-gun, Korea

The Korean Journal of Parasitology ◽

10.3347/kjp.2021.59.1.83 ◽

2021 ◽

Vol 59 (1) ◽

pp. 83-88

Author(s):

Bong-Kwang Jung ◽

Taehee Chang ◽

Hyejoo Shin ◽

Seungwan Ryoo ◽

Sooji Hong ◽

...

Keyword(s):

Life Cycle ◽

Post Infection ◽

Ruditapes Philippinarum ◽

Manila Clam ◽

Long Tail ◽

5.8S Rdna ◽

Rdna Its2 ◽

Life Cycle Stages ◽

Second Intermediate Host

Life cycle stages, including daughter sporocysts, cercariae, and metacercariae, of Parvatrema duboisi (Dollfus, 1923) Bartoli, 1974 (Digenea: Gymnophallidae) have been found in the Manila clam Ruditapes philippinarum from Aphaedo (Island), Shinan-gun, Jeollanam-do, Korea. The daughter sporocysts were elongated sac-like and 307-570 (av. 395) μm long and 101-213 (av. 157) μm wide. Most of the daughter sporocysts contained 15-20 furcocercous cercariae each. The cercariae measured 112-146 (av. 134) μm in total length and 35-46 (av. 40) μm in width, with 69-92 (av. 85) μm long body and 39-54 (av. 49) μm long tail. The metacercariae were 210-250 (av. 231) μm in length and 170-195 (av. 185) μm in width, and characterized by having a large oral sucker, genital pore some distance anterior to the ventral sucker, no ventral pit, and 1 compact or slightly lobed vitellarium, strongly suggesting P. duboisi. The metacercariae were experimentally infected to ICR mice, and adults were recovered at day 7 post-infection. The adult flukes were morphologically similar to the metacercariae except in the presence of up to 20 eggs in the uterus. The daughter sporocysts and metacercariae were molecularly (ITS1-5.8S rDNA-ITS2) analyzed to confirm the species, and the results showed 99.8-99.9% identity with P. duboisi reported from Kyushu, Japan and Gochang, Korea. These results confirmed the presence of various life cycle stages of P. duboisi in the Manila clam, R. philippinarum, playing the role of the first as well as the second intermediate host, on Aphae-do (Island), Shinan-gun, Korea.

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Sibling Species of Cercospora Associated with Gray Leaf Spot of Maize

Phytopathology ◽

10.1094/phyto.1998.88.12.1269 ◽

1998 ◽

Vol 88 (12) ◽

pp. 1269-1275 ◽

Cited By ~ 46

Author(s):

Juan Wang ◽

Morris Levy ◽

Larry D. Dunkle

Keyword(s):

United States ◽

Genetic Distance ◽

Sibling Species ◽

Fragment Length ◽

Leaf Spot ◽

The United States ◽

Nucleotide Sequences ◽

Gray Leaf Spot ◽

5.8S Rdna ◽

Its Regions

Monoconidial isolates of the fungus causing gray leaf spot of maize were obtained from diseased leaves collected throughout the United States and analyzed for genetic variability at 111 amplified fragment length polymorphism (AFLP) loci. Cluster analysis revealed two very distinct groups of Cercospora zeae-maydis isolates. Both groups were found to be relatively uniform internally with an average genetic similarity among isolates of approximately 93 and 94%, respectively. The groups were separated from each other by a genetic distance of approximately 80%, a distance greater than that separating each group from the sorghum pathogen, C. sorghi (67 to 70%). Characteristics and dimensions of conidia and conid-iophores produced on infected plants or nutrient media were unreliable criteria for taxonomic differentiation of isolates composing the two groups of C. zeae-maydis. Nucleotide sequences of 5.8S ribosomal DNA (rDNA) and the internal transcribed spacer (ITS) regions were identical within each group but different between the two groups and different from C. sorghi. Restriction fragment length polymorphisms generated by digestion of the 5.8S rDNA and ITS regions with TaqI readily distinguished each group and C. sorghi. Isolates in one group were generally distributed throughout maize-producing regions of the United States; isolates in the other group were localized in the eastern third of the country. Both types were present in the same fields at some locations. The genetic distance based on AFLP profiles and different ITS nucleotide sequences between the two morphologically indistinguishable groups indicate that they are sibling species. Although it is unlikely that breeding for resistance to gray leaf spot will be confounded by local or regional variation in the pathogen, a vigilant approach is warranted, because two pathogenic species exist with unknown abilities to evolve new pathotypes.

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Microtrema barusi gen. n. and sp. n. (Digenea: Phaneropsolidae) from Prunella modularis (Aves, Passeriformes) in the Czech Republic

Helminthologia ◽

10.2478/s11687-013-0106-6 ◽

2013 ◽

Vol 50 (1) ◽

pp. 43-45 ◽

Cited By ~ 2

Author(s):

J. Sitko

Keyword(s):

Czech Republic ◽

Ventral Sucker ◽

Oral Sucker ◽

Genital Pore ◽

Short Distance ◽

Posterior Part ◽

Excretory Vesicle ◽

The Czech Republic

AbstractMicrotrema gen. n. and Microtrema barusi sp. n. are described from the intestine of Prunella modularis in the Czech Republic. Genus and species have the following features: Body claviform, widest in region of ventral sucker. Tegument densely covered with tiny spines. Suckers small, widely separated. Oral sucker globular, subterminal. Ventral sucker globular, equatorial. Prepharynx very short or absent. Pharynx small, fine. Oesophagus divided into two short intestinal branches ending blindly anterior to testes. Testes globular, lateral, in region of ventral sucker. Vesicula seminalis small, genital pore in short distance before ventral sucker and opening of uterus. Bursa cirri and cirrus absent. Ovary globular, median, between testes. Mehlis’ gland long oval, posterior to ovary. Vitellarium weakly developed, in two lateral clusters composed of small follicles. Uterus in posterior part of body, with many eggs, covering vitellarium and partly ventral sucker. Excretory vesicle V-shaped, pore terminal.

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Prevalence of Rumen and Reticulum Trematodes in The Goat Slaughtered at Pegirian Surabaya Slaughter House using Digestive Surgery Method

Journal of Parasite Science ◽

10.20473/jops.v3i2.16525 ◽

2019 ◽

Vol 3 (2) ◽

pp. 89

Author(s):

Novia Intan Kurnia ◽

Setiawan Koesdarto ◽

Herry Agoes Hermadi ◽

Kusnoto Kusnoto ◽

Hardany Primarizky ◽

...

Keyword(s):

Ventral Sucker ◽

Oral Sucker ◽

Genital Pore ◽

Staining Method ◽

Chi Square ◽

Digestive Surgery ◽

Slaughter House ◽

Paramphistomum Cervi

The purpose of this study was to determine the kind of trematodes which infected the rumen and reticulum of goat slaughtered at RPH Pegirian Surabaya using digestive surgery method, and determine the prevalence result. 36 samples of rumen and reticulum were collected from RPH Pegirian from September - November 2017 then using digestive surgery to obtain fresh adult worms, afterwards using the Semichen-Acetic Carmine staining method to makes the object easy to identifying. The results showed that species was found on goat was Paramphistomum cervi and Cotylophoron cotylophorum, in the Paramphistomum cervi are several characters wich is oral sucker, genital pore, uterus, testis, ventral sucker and in Cotylophoron cotylophorum contain oral sucker, branched oesophagus, uterus, vitelin glands, testes , posterior sucker. 3 rumen positive trematodosis with prevalence of 8.33% Chi-square statistic test (p>0.05) showed no effect between goat species and Trematodosis prevalence, 20 kacang goat and 16 peranakan etawa goat found 2 positive kacang goat (10%) and 1 peranakan etawa goat positive (6.25%).

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