Cryptocotyle lata (Trematoda: Heterophyidae) Adult from a Korean Raccoon Dog, Nyctereutes procyonoides koreensis

Total 513 heterophyid flukes were collected from a carcass of wild Korean raccoon dog, Nyctereutes procyonoides koreensis, in Korea. With morphological and molecular characteristics, the flukes were identified to Cryptocotyle lata. The adult C. lata were minute, transparent, pentagonal, 522 µm long by 425 µm wide. Ceca extended into post-testicular region. Ventrogenital sac elliptical, 79 µm by 87 µm with genital pore and ventral sucker. Two testes semielliptical and slightly lobed, located in the posterior region, right testis 173 µm by 155 µm, left testis 130 µm by 134 µm. In a phylogenetic tree, the fluke specimen of this study was grouped with C. lata divergent from Cryptocotyle lingua. We report here N. procyonoides koreensis first as a natural definitive host of C. lata.

Euryhelmis squamula (Digenea: Heterophyidae) Recovered from Korean Raccoon Dog, Nyctereutes procyonoides koreensis, in Korea

In this study, we intended to describe an unrecorded species of heterophyid trematode recovered from the small intestine of a Korean raccoon dog, Nyctereutes procyonoides koreensis, in Korea. A total of 13 small flukes were collected from a deceased Korean raccoon dog which was found in Chuncheon-si, Gangwon-do, Korea in May 2017. The trematode body were covered with many small spines, rectangular, broader than long, 807-1,103 µm long and 1,270-1,550 µm wide. Oral sucker in the anterior end slightly smaller than acetabulum. Pharynx muscular and well developed. Esophagus relatively long and sigmoid. Acetabulum small and located at median in anterior 2/5 portion. Ceca bifurcated at the anterior of genital pore and acetabulum and terminated at testis level. Testes larger, deeply lobed and located at the near of posterior end of body. Ovary small, triangular and located at the slight left of median and the anterior of left testis. Vitelline follicles dendritic and extend from the middle level of esophagus to the posterior portion of body. Eggs embryonated, operculated, small and 33-35×15-16 µm in size. Based on the morphological characteristics, the small heterophyid flukes recovered from the small intestines of Korean raccoon dog, N. procyonoides koreensis, were identified as Euryhelmis squamula (Digenea: Heterophyidae). Accordingly, this species of heterophyid flukes is to be a new trematode fauna in Korea by this study.

A new order of fishes as hosts of blood flukes (Aporocotylidae); description of a new genus and three new species infecting squirrelfishes (Holocentriformes, Holocentridae) on the Great Barrier Reef

A new genus and three new species of blood flukes (Aporocotylidae) are described from squirrelfishes (Holocentridae) from the Great Barrier Reef. Holocentricola rufus n. gen., n. sp. is described from Sargocentron rubrum (Forsskål), from off Heron Island, southern Great Barrier Reef, and Lizard Island, northern Great Barrier Reef, Australia. Holocentricola exilis n. sp. and Holocentricola coronatus n. sp. are described from off Lizard Island, H. exilis from Neoniphon sammara (Forsskål) and H. coronatus from Sargocentron diadema (Lacepède). Species of the new genus are distinct from those of all other aporocotylid genera in having a retort-shaped cirrus-sac with a distinct thickening at a marginal male genital pore. The new genus is further distinct in the combination of a lanceolate body, X-shaped caeca, posterior caeca that are longer than anterior caeca, a single, post-caecal testis that is not deeply lobed, a post-caecal, post-testis ovary that is not distinctly bi-lobed, and a post-ovarian uterus. The three new species can be morphologically delineated based on the size and row structure of the marginal spines, as well by total length, oesophagus and caecal lengths, and the position of the male genital pore, testes and ovary relative to the posterior extremity. The three species of Holocentricola are genetically distinct from each other based on cox1 mtDNA and ITS2 rDNA data, and in phylogenetic analyses of 28S rDNA form a well-supported clade sister to species of Neoparacardicola Yamaguti, 1970. This is the first report of aporocotylids from fishes of the family Holocentridae and the order Holocentriformes.

Morphological and Molecular Confirmation of Parvatrema duboisi Metacercariae in the Manila Clam Ruditapes philippinarum from Gochang-gun, Korea

Gymnophallid metacercariae found in the Manila clam <i>Ruditapes philippinarum</i> (‘Banjirak’ in Korean) from Gochang-gun, Jeollabuk-do, Korea were morphologically and molecularly confirmed to be <i>Parvatrema duboisi</i> (Dollfus, 1923) Bartoli, 1974. The metacercariae were morphologically characterized by having a large oral sucker, small ventral sucker, genital pore some distance anterior to the ventral sucker, no ventral pit, and 1 compact or slightly lobed vitellarium, which were all compatible with <i>P. duboisi</i>. Some of the metacercariae were experimentally fed to mice, and adult flukes were recovered at day 7 post-infection. The morphology of the adult flukes was basically the same as that of the metacercariae except for the presence of uterine eggs; the uterus was filled with up to 40 eggs. The nucleotide sequences (1,193 bp) from ITS regions (ITS1, 5.8S rDNA, and ITS2) of the metacercariae showed 99.7% identity with <i>P. duboisi</i> and 75.7% identity with <i>Gymnophalloides seoi</i> deposited in GenBank. These results confirmed the presence of <i>P. duboisi</i> metacercariae in the Manila clam <i>R. philippinarum</i> in an estuary region of Gochang-gun, Korea.

Prevalence of Rumen and Reticulum Trematodes in The Goat Slaughtered at Pegirian Surabaya Slaughter House using Digestive Surgery Method

The purpose of this study was to determine the kind of trematodes which infected the rumen and reticulum of goat slaughtered at RPH Pegirian Surabaya using digestive surgery method, and determine the prevalence result. 36 samples of rumen and reticulum were collected from RPH Pegirian from September - November 2017 then using digestive surgery to obtain fresh adult worms, afterwards using the Semichen-Acetic Carmine staining method to makes the object easy to identifying. The results showed that species was found on goat was Paramphistomum cervi and Cotylophoron cotylophorum, in the Paramphistomum cervi are several characters wich is oral sucker, genital pore, uterus, testis, ventral sucker and in Cotylophoron cotylophorum contain oral sucker, branched oesophagus, uterus, vitelin glands, testes , posterior sucker. 3 rumen positive trematodosis with prevalence of 8.33% Chi-square statistic test (p>0.05) showed no effect between goat species and Trematodosis prevalence, 20 kacang goat and 16 peranakan etawa goat found 2 positive kacang goat (10%) and 1 peranakan etawa goat positive (6.25%).

Five new species of Acanthobothrium (Cestoda: Onchoproteocephalidea) from the long-tailed butterfly ray, Gymnura cf. poecilura (Elasmobranchii: Gymnuridae), from the Persian Gulf and Gulf of Oman

Five new species of Acanthobothrium Blanchard, 1848 are described from Gymnura cf. poecilura from the Gulf of Oman and Persian Gulf. They all belong to the Category 1 and can be differentiated from all congeners by a combination of characters, including marginal lappets on the bothridial rim, the lack of spinitrich microtriches on the proximal bothridial surfaces, the position of the genital pore in the posterior one fifth of the proglottid, the direction of the cirrus sac parallel and clinging to the ovarian lobe, the lack of post-vaginal testes, and the interruption of the vitelline follicles by the ovary. The five new species are morphologically similar to each other but differ among each other in their cephalic peduncle length, proglottid and testes number, and the apolysis status. The most similar species to this new group is Acanthobothrium fogeli Gloldstein, 1964 from the Gulf of Mexico. The new species differ from A. fogeli by the muscular pad size, cephalic peduncle length and having marginal lappets on the bothridial rim. the species of Acanthobothrium occurs in three families of elasmobranchs in the Gulf of Oman and Persian Gulf (Dasyatidae, Rhynchobatidae and Gymnuridae). The true identity of many hosts in the region is ambiguous. Therefore, we designated the sampled elasmobranch as G cf. poecilura in accordance to the previously molecular study on a few individuals from the region.

Study of morphology on newly found Heamphysalis concinna tick at Selenge province

The blood sucking 4 species ticks of Haemaphysalis genus were distributed in Mongolia, that an important vectors of diseases transmitting to human and animals and causing economic lost. Newly found Haemaphysalis females in Selenge province was confirmed to H. concinna species based on following features: strong lateral margins of palp article II, heart-shaped scutum with widest at middle, short-wide hypostome, sharpen cornua of basis capituli, posterior margins of basis capituli, ventral surface of basis capituli, latero-anterial I festoon connected with lateral, anterior groove of genital pore and additional spur of leg I trochanter. To confirm morphological description of H. concinna, CoxI sequence analysis was done and results was showed H. concinna CoxI sequences in GenBank with 86-89% identity. The results indicate that the Haemaphysalis concinna tick was distributed in Mongolia and it is announcing that the spread of Haemaphysalis tick in Selenge aimag. Сэлэнгэ аймгийн нутгаас шинээр олдсон Heamphysalis concinna хачгийн хэлбэр зүйн судалгаа Хураангуй: Монгол оронд Haemaphysalis төрлийн 4 зүйлийн хачиг тархан хүн, мал амьтанд шимэгчилж өвчин дамжуулан хохирол учруулдаг. Сэлэнгэ аймгийн нутгаас олдсон хачиг нь хэлбэр зүйн ерөнхий онцлогоороо Haemaphysalis төрөлд хамаарагдаж байлаа. Хошууны пальпын II үеийн хажуугийн нэмэлт хурц ирмэг, бамбайн дунд хэсгээрээ өргөссөн зүрх мэт хэлбэр, богино өргөн гипостом, хошууны суурийн булангийн эвэр, арын ирмэгийн илрэлт, хошууны суурийн доод гадаргуугийн хэлбэр, хачгийн захын I фестон нь зөвхөн биеийн хажуугийн ховилтой холбогдсон байдал, бэлгийн сүвийн урьдах зураасны онцлог, бэлгийн сүвийн хэлбэрийн онцлог, ялгадасны сүвийн арын ховилын онцлог, I хөлийн I үеийн нэмэлт өргөс хэлбэрийн өргөсөлт зэрэг онцлогоороо Haemaphysalis concinna зүйлийн хачиг болох нь батлагдав. Энэхүү хачгийн морфологийн ялган танилтыг CохI генийн нуклеотидын дарааллыг тогтоон баталгаажуулахад ГенБанкинд бүртгэлтэй H. concinna хачгийн нуклеотидын дарааллуудтай 86-89%-ийн адил байлаа. Бидний судалгааны үр дүнд хэлбэр зүйн онцлог, нуклеотидын дараалал тогтоох шинжилгээгээр Монгол оронд H. concinna зүйл хачиг тархсан нь тогтоогдсон тул Монгол орны хачгийн жагсаалтанд оруулж болохыг баталгаажуулж байна. Мөн энэхүү судалгаагаар Сэлэнгэ аймгийн нутгаас Haemaphysalis төрлийн хачиг анх удаа илрүүлэв. Түлхүүр үг: Цус сорогч хачиг, Haemaphysalis concinna, хэлбэр зүй, Сэлэнгэ аймаг

Description of a new species Fuhrmannetta jurubatensis n. sp. (Cestoda, Davaineidae) from Cerradomys goytaca Tavares, Pessôa & Gonçalves, 2011 (Rodentia, Cricetidae)

A new species of cestode of the genus Fuhrmannetta found in the small intestine of Cerradomys goytaca is described herein, named Fuhrmannetta jurubatensis n. sp. Rodents were collected from the sand-plains areas of the northern coast of the State of Rio de Janeiro, Brazil. Morphological analyses were conducted by light and scanning electron microscopy. From the morphological analysis and a comparison with the known species of the genus, F. jurubatensis n. sp. can be identified by a combination of morphological and morphometrical characteristics, including strobila length, number and length of rostellar hooks, position of the genital pore and the number of eggs per uterine capsule.

GLOSSIPHONIA BALCANICA N. SP. AND DINA PROKLETIJACA N. SP. (HIRUDINIDA: GLOSSIPHONIIDAE, ERPOBDELLIDAE) - TWO NEW LEECHES FROM MONTENEGRO AND KOSOVO

Two new leech species (Hirudinida) representing the families Glossiphoniidae (Glossiphonia balcanica n. sp.) and Erpobdellidae (Dina prokletijaca n. sp.) are described. The populations from Montenegro assigned by Utevsky et al. (2013) and Grosser et al. (2015) to Glossiphonia nebulosa Kalbe, 1964 represent an new species, here described as G. balcanica n. sp. The new species can be easily be separated from Glossiphonia nebulosa by the reduction of the papillae. Dina prokletijaca n. sp. closely resembles D. dinarica Sket, 1968 and D. montana Sket, 1968 from which can be distinguished in the combination of the small and stocky body, dorsal surface with two wide and dark paramedian longitudinal stripes and ovisacs reaching the fourth somite after the female genital pore, and curled in their entire course. A key to the species of Glossiphonia Johnson, 1816 and Dina R. Blanchard, 1892 from the Western Balkans is presented.

Two new species of Rhinebothrium (Cestoda: Rhinebothriidea) from granulated guitarfish Glaucostegusgranulatus in the Gulf of Oman

During a study of the rhinebothriideans of rays in the Gulf of Oman, two new species of Rhinebothrium Linton, 1890, Rhinebothrium kruppi sp. n. and R. persicum sp. n., were identified in Glaucostegus granulatus (Cuvier). Some significant features that distinguish R.kruppi sp. n. from R. persicum sp. n. include: scolex characteristics (hinged with 42–46 loculi vs. fusiform with 68–62 loculi), number of testes (4–5 vs. 20–27), genital pore position (61.1–76.9% of proglottid length vs. 47.2–63.3%), ovarian morphology (lobulated vs. follicular), cirrus-sac expansion (past midline of proglottid vs. limited to poral side of proglottid), vas deferens configuration (spanning posteriorly to near ovarian isthmus vs. to the level of ovarian anterior margins) and details of microthrix morphology. In addition, a combination of the aforementioned characteristics can be used to distinguish these two new species from other valid species of Rhinebothrium. These are the first species of rhinebothriidean cestodes to be described systematically from the Gulf of Oman, Iran. The two new species reported here increase the number of valid species of Rhinebothrium to 43.