scholarly journals Breeding for resistance to tomato bacterial wilt: Identification of potential sources of genetic resistance by field evaluation of 28 tomato cultivars in Daloa, Côte d’Ivoire

2020 ◽  
Vol 8 (12) ◽  
pp. 305-313
Author(s):  
N’Guessan Olivier Konan ◽  
◽  
Yao Patrice Houphouet ◽  
Ibrahim Konaté ◽  
Saïdou Diallo Mamadou ◽  
...  

Bacterial wilt caused by Ralstonia solanacearum is one of the most damaging tomato diseases in the world. The best strategy for controlling this disease is to use genetically resistant tomato plants. In this study, 28 tomato cultivars were evaluated in the field for their resistance to bacterial wilt in Daloa, one of the most important agricultural regions of Côte d'Ivoire. The experiments were carried out during the dry and rainy season and the experimental design was a Randomized Complete Block Design (RCBD) with three replications. The results showed the presence of wilt disease. The streaming test and bacterial culture on growth medium proved the bacterial origin of the wilt symptoms observed. The results of the staining reactions revealed short, straight, rod and Gram negative bacteria similar to R. solanacearum. The severity of the disease was found to be higher in the dry season than in the rainy season. Of 28 cultivars evaluated, 7 cultivars were resistant and did not show any diseased plants during the two study periods. These cultivars constitute interesting sources of resistance for a breeding program aimed at the development of new resistant cultivars adapted to the region of Daloa. Keywords: Bacterial wilt, Ralstonia solanacearum, resistance, tomato, Solanum lycopersicum, breeding program.

2016 ◽  
Vol 11 (1) ◽  
pp. 18-25
Author(s):  
Ouattara Genefol ◽  
Camara Brahima ◽  
Bomisso Edson Lezi ◽  
Cherif Mamadou ◽  
Sorho Fatogoma ◽  
...  

2019 ◽  
Vol 41.2 ◽  
pp. 6926-6939
Author(s):  
NIAMIEN Coffi Jean Magloire ◽  
KONAN Ekoun Michaël ◽  
ODOUKPE Kadio Saint Guillaume ◽  
YAOKOKORE-BEIBRO Kouassi Hilaire ◽  
N’GORAN Kouakou Eliézer

Les oiseaux rendent d’importants services écologiques nécessaires au bien-être de l’homme. En Côte d’Ivoire, l’essentiel des études ornithologiques se sont déroulées au sud, et accessoirement au centre et au centre-ouest. En outre, très peu de travaux ont porté sur les zones humides. La ville de Korhogo abrite des barrages à vocation agro-pastorale qui n’ont été l’objet d’aucune étude. Pour combler ce manque de connaissance, une étude a été menée de février 2016 à janvier 2017. L’objectif de ce travail est d’évaluer la richesse spécifique et de déterminer l’influence des saisons sur la distribution qualitative de la communauté des oiseaux du barrage urbain de Koko dans la commune de Korhogo. Pour ce faire, des observations directes, utilisant la méthode des transects ponctuée d’arrêts d’environ 15 minutes, ont été effectuées. Les résultats indiquent que cette communauté est constituée de 63 espèces d’oiseaux appartenant à 32 familles et regroupées en 14 ordres. Globalement, l’ordre des Passériformes et la famille des Ardeidae sont les mieux représentés. Du point de vue de leur statut biogéographique, les espèces résidentes (37 individus : 59 %) et accidentelles (23 individus : 36,51%) sont majoritaires. Le plus grand nombre d’espèces a été observé en saison des pluies (52 individus : 82,54%). Sept espèces ont été spécifiques de la saison sèche alors que trois espèces sont caractéristiques de la saison des pluies. Les espèces résidentes ont été les nombreuses en toute saison. Au niveau structural, les espèces accessoires, les espèces assez-fréquentes et les espèces fréquentes ont été les mieux représentées en saison sèche tandis que les espèces assez-fréquentes ont été majoritaires en saison des pluies. Cette étude confirme la distribution des communautés des oiseaux selon les saisons. ABSTRACT Birds provide important ecosystem services necessary for human well-being. In Côte d'Ivoire, ornithological studies were carried out in the south, central and central-west. In addition, very little work has been done on wetlands. The Commune of Korhogo is home to agro-pastoral dams that have not been studied. To fill in this gap of information, a study was conducted from February 2016 to January 2017. The aim of this study was to assess the specific richness and to examine the effect of seasons on the qualitative distribution of the Koko urban dam birds’ community. Direct observations using the method of line transects with a 15 minutes points were carried out. Results show that this community consists of 63 species of birds belonging to 32 families and grouped in 14 orders. Globally, the order of the Passeriformes and the family of Ardeidae were the best represented. In terms of their biogeographical status, resident species (37 individuals: 59%) and accidental species (23 individuals: 36.51%) were dominant. The high number of bird species was observed during the rainy season (52 individuals: 82.54%). Seven species were specific to the dry season while three species are characteristic of the rainy season. Resident species were numerous in all seasons. The accessory and relatively frequent species have been the most abundant during the dry season while the fairly frequent species were predominant in the rainy season. This study confirms the distribution of bird communities according to the seasons.


2012 ◽  
Vol 102 (8) ◽  
pp. 733-740 ◽  
Author(s):  
C. A. N'Guessan ◽  
K. Abo ◽  
L. Fondio ◽  
F. Chiroleu ◽  
A. Lebeau ◽  
...  

The genetic and phenotypic diversity of Côte d'Ivoire Ralstonia solanacearum strains was assessed on a 168-strain collection sampled on Solanaceae both in the southern lowlands and western highlands. Phylotypes I, II, and III were prevalent, though at unexpected frequencies. Phylotype I strains (87.5%) were genetically diverse and overrepresented in all agroecological areas, including highlands (AEZ III). Phylotype II strains (10.7%) only belonged to one tropical lowland-adapted broad host range lineage (IIA-35), whereas no highland-adapted potato brown rot (IIB-1) or Moko strains were detected. African phylotype III strains were rare (1.8%). They originated from a single Burkina Faso lineage (III-23) and were only found in lowlands. Three phylotype I strains were found harboring pRSC35, a plasmid identified in phylotype III strains in Cameroon. From pathogenicity tests performed on commercial varieties and tomato/eggplant/pepper references, the virulence diversity observed was high, with five pathoprofiles described. Eggplant accessions MM152 and EG203 and tomato HW7996 displayed the largest resistance spectrum and highest level. Two highly virulent phylotype I strains were able to bypass resistance of HW7996 and the eggplant reference AG91-25. Collectively, these points lead to the conclusion that the situation in Côte d'Ivoire is specific towards other African countries, and specifically from the Cameroon reference, and that within phylotype I can exist a high virulence diversity. This calls for similar studies in neighboring West African countries, linking R. solanacearum pathogen genetic diversity to strain virulence at the regional level, for the rationalization of regional resistance deployment strategies and future resistance durability studies.


Parasite ◽  
2019 ◽  
Vol 26 ◽  
pp. 40 ◽  
Author(s):  
Barnabas Zogo ◽  
Dieudonné Diloma Soma ◽  
Bertin N’Cho Tchiekoi ◽  
Anthony Somé ◽  
Ludovic P. Ahoua Alou ◽  
...  

A better understanding of malaria transmission at a local scale is essential for developing and implementing effective control strategies. In the framework of a randomized controlled trial (RCT), we aimed to provide an updated description of malaria transmission in the Korhogo area, northern Côte d’Ivoire, and to obtain baseline data for the trial. We performed human landing collections (HLCs) in 26 villages in the Korhogo area during the rainy season (September–October 2016, April–May 2017) and the dry season (November–December 2016, February–March 2017). We used PCR techniques to ascertain the species of the Anopheles gambiae complex, Plasmodium falciparum sporozoite infection, and insecticide resistance mechanisms in a subset of Anopheles vectors. Anopheles gambiae s.l. was the predominant malaria vector in the Korhogo area. Overall, more vectors were collected outdoors than indoors (p < 0.001). Of the 774 An. gambiae s.l. tested in the laboratory, 89.65% were An. gambiae s.s. and 10.35% were An. coluzzii. The frequencies of the kdr allele were very high in An. gambiae s.s. but the ace-1 allele was found at moderate frequencies. An unprotected individual living in the Korhogo area received an average of 9.04, 0.63, 0.06 and 0.12 infected bites per night in September–October, November–December, February–March, and April–May, respectively. These results demonstrate that the intensity of malaria transmission is extremely high in the Korhogo area, especially during the rainy season. Malaria control in highly endemic areas such as Korhogo needs to be strengthened with complementary tools in order to reduce the burden of the disease.


Author(s):  
Kouakou Marius Konan ◽  
Sifolo Seydou Coulibaly ◽  
Kouadio Ignace Kouassi ◽  
Bi Irié Arsène Zoro ◽  
Dénezon Odette Dogbo

This study aimed to evaluate the effect of compost from chicken manure, NPK fertilizer and the mixture of both fertilizers on agronomic parameters of four varieties of cassava (Yacé, Bonoua, Six mois and Alleda agba) in Côte d’Ivoire. The field experiment was carried out under a randomized complete block design with three replicates on a ferralitic soil during two season-cycles. The results showed that the different fertilizers stimulated plants growth and tuberous roots production. The highest number of stems (3) and leaves (66) was obtained with the mixture of NPK and compost applied on the variety “Bonoua”. All varieties that received the compost mixed with NPK produced more leaves with a dominance of the Bonoua variety (65.57). The highest number of tuberous roots (5.58), the heaviest weight per root (6.13 kg) and the highest yield (61.37 t/ha) were obtained while using the mixture of compost and NPK as fertilizer and “Bonoua” as variety. Thus, mixing compost from chicken manure and NPK can be recommended to improve cassava productivity in Côte d'Ivoire on a ferralitic soil.


2021 ◽  
Vol 11 (2) ◽  
pp. 313-320
Author(s):  
Mohamed Doumbouya ◽  
Anderson Mohamed Yeo ◽  
Carine Aya N’Guessan ◽  
Arthur Martin Affery ◽  
Daouda Koné

Bacterial wilt caused by R. solanacearum is a constraint that limits the production of solanaceous in Côte d'Ivoire. The genetic control, which is more widely used, comes up against the genetic and phenotypic variability of the strains. The objective of this study was to screen commercial and traditional varieties of Solanaceae against Ivorian strains of different phylotypes to assess their spectrum of resistance to the disease. The level of virulence of the five strains of R. solanacearum was evaluated in semi-controlled conditions against eight varieties of Solanaceae (pepper, eggplant and tomato). Four groups of strains were identified according to the level of virulence. Group 4, composed of strains (RUN 1794 and RUN 1854) of phylotype III and I, respectively, and originating from the localities of Kondoukro and Yamoussoukro, was very virulent. The resistant varieties were for the F1 kalenda eggplant, for the yellow chilli from Burkina and for the F1 Lindo tomato. These varieties could be used in the fight against bacterial wilt in Côte d'Ivoire.


Author(s):  
Kouadio Koffi Patrice ◽  
Bongoua-Devisme Affi Jeanne ◽  
Yoboué Kouadio Emile ◽  
Touré Nantarie ◽  
Beugré Christian Moise ◽  
...  

This study consists of using the cocoa bean teguments as a means of sustainable production growth and environmental preservation. It aims to characterize the effects of the teguments on the chemical properties of a Ferralsol in Ahoué (Côte d'Ivoire). In an experimental block design of Fisher, three treatments of Cocoa bean teguments: T1 (10 t.ha-1), T2 (15 t.ha-1), T3 (20 t.ha-1) and one Control T0 have been set up with four repetitions. The main results show that the contribution of cocoa bean teguments has a positive effect on all the characteristics studied. The values obtained of the pH of the T0 and the T1, T2, T3 indicates, respectively 4.2; 5.4; 5.5 and 5.8. As regards the contents of the CEC, the values obtained give: 2.32; 7.2; 11.44 and 12.5 cmol.kg-1, respectively, for T0, T1, T2 and T3 treatments. As for the organic matter, the contents obtained indicate the following values: T0 = 12.56 g.kg-1; T1 = 26.49 g.kg-1; T2 = 35.09 g.kg-1 and T3 = 38.08 g.kg-1. The data corresponding to the sum of the captions oscillated between 4.5 and 10.5 cmol.kg-1 according to the treatments.


2018 ◽  
Vol 11 (1) ◽  
pp. 2201-2217
Author(s):  
Dr. NDRI Kouadio Julien ◽  
Arnauth M. Guaci ◽  
Thierry Hance ◽  
Joseph G. Yaco ◽  
Jean-Luc D.S. Ahui ◽  
...  

Due to their interaction with many other small Arthropods, Uropodina mites can be considered as good indicators of soil fauna of forest litter. In order, to better understand their distribution and phenology according to forest type four sites from primary forest to plantations were sampled in 2008 in Côte d’Ivoire: 1- the Lamto savannah (6°13' N, 5°02' W), 2- Oumé primary forest (6°31’ N, 5°30’ W), 3- Oumé teak plantation (6°31’ N, 5°30’ W) all situated in the Sudanese domain and finally, 4- the Taï primary forest (5°45’ N, 7°07 W) located in the Guinean domain. After a preliminary study devoted to the efficiency of Berlese Tullgren funnel, the spatiotemporal variation of two Uropodina genera - Afrotrachytes Kontschán, 2006 and Trachyuropoda Berlese, 1888 - was assessed. We hypothesized that the abundance of Uropodina would be higher in primary forest and lower in savannah and monospecific plantation. Whatever the season, we expected that the abundance of Uropodina would decrease with soil depth and would vary along transect. On each site, 15 sampling points were allocated over a 14-m transect with 1m intervals between two consecutive points. For each sampling point, 9 cores (litter, 0-5, 5-10, 10-15, 15-20, 20-25, 25-30, 30-35 and 35-40 cm) were taken with a steel corer (Ø 3.5 cm). Thus, a total of 1,080 soil cores were collected over two sampling periods from January to March 2008 (dry season) and August to October 2008 (rainy season). Soil physico-chemical parameters were also characterized. Mites were extracted using the Berlese-Tullgren funnels for one week after testing the extraction duration in a preliminary study. The bulbs lighting as soon as the soil cores were placed in Berlese Tullgren gave better results regarding the abundance of extracted mites. The results showed that the abundance of Afrotrachytes sp and Trachyuropoda sp was higher in rainy season, and varied significantly through the sites, whatever the season. The highest abundances of Afrotrachytes sp were observed in Oumé primary forest whereas those of Trachyuropoda sp were recorded in Oumé primary forest, and in Lamto savannah, whatever the season. Apart from the distribution of Trachyuropoda sp in dry season, the abundance of Afrotrachytes sp and Trachyuropoda sp was greater in the topsoil (litter and 0-5 cm) and decreased with soil depth. The abundances of Afrotrachytes sp and Trachyuropoda sp did not follow a normal distribution along the transects. The season-soil depth interaction affected significantly the abundance of Trachyuropoda sp whereas the bulk density (dry season and rainy season), soil depth (dry season), carbon / nitrogen ratio (dry season) impacted significantly the abundance of Afrotrachytes sp. This first study highlighted the spatiotemporal variation of Uropodina in Côte d’Ivoire. However, taking into account of the different dispersal agents in future studies would help us to better understand their abundance and distribution along different habitats, as well as their role as biological control agents.


Sign in / Sign up

Export Citation Format

Share Document