In situ 13C tracer experiments elucidate carbon translocation rates and allocation patterns in eelgrass Zostera marina

2013 ◽  
Vol 487 ◽  
pp. 27-39 ◽  
Author(s):  
JE Kaldy ◽  
CA Brown ◽  
CP Andersen
2006 ◽  
Vol 29 (1) ◽  
pp. 467-467
Author(s):  
Hidetaka Nomaki ◽  
P Heinz ◽  
N Ohkouchi ◽  
T Nakatsuka ◽  
H Suga ◽  
...  

2014 ◽  
Vol 11 (14) ◽  
pp. 3729-3738 ◽  
Author(s):  
K. E. Larkin ◽  
A. J. Gooday ◽  
C. Woulds ◽  
R. M. Jeffreys ◽  
M. Schwartz ◽  
...  

Abstract. Foraminifera are an important component of benthic communities in oxygen-depleted settings, where they potentially play a significant role in the processing of organic matter. We tracked the uptake of a 13C-labelled algal food source into individual fatty acids in the benthic foraminiferal species Uvigerina ex. gr. semiornata from the Arabian Sea oxygen minimum zone (OMZ). The tracer experiments were conducted on the Pakistan margin during the late/post monsoon period (August–October 2003). A monoculture of the diatom Thalassiosira weisflogii was 13C-labelled and used to simulate a pulse of phytoplankton in two complementary experiments. A lander system was used for in situ incubations at 140 m water depth and for 2.5 days in duration. Shipboard laboratory incubations of cores collected at 140 m incorporated an oxystat system to maintain ambient dissolved oxygen concentrations and were terminated after 5 days. Uptake of diatoms was rapid, with a high incorporation of diatom fatty acids into foraminifera after ~ 2 days in both experiments. Ingestion of the diatom food source was indicated by the increase over time in the quantity of diatom biomarker fatty acids in the foraminifera and by the high percentage of 13C in many of the fatty acids present at the endpoint of both in situ and laboratory-based experiments. These results indicate that


2015 ◽  
Vol 45 (2) ◽  
pp. 407-423 ◽  
Author(s):  
Thomas Meunier ◽  
Claire Ménesguen ◽  
Richard Schopp ◽  
Sylvie Le Gentil

AbstractThe dynamics of the formation of layering surrounding meddy-like vortex lenses is investigated using primitive equation (PE), quasigeostrophic (QG), and tracer advection models. Recent in situ data inside a meddy confirmed the formation of highly density-compensated layers in temperature and salinity at the periphery of the vortex core. Very high-resolution PE modeling of an idealized meddy showed the formation of realistic layers even in the absence of double-diffusive processes. The strong density compensation observed in the PE model, in good agreement with in situ data, suggests that stirring might be a leading process in the generation of layering. Passive tracer experiments confirmed that the vertical variance cascade in the periphery of the vortex core is triggered by the vertical shear of the azimuthal velocity, resulting in the generation of thin layers. The time evolution of this process down to scales of O(10) m is quantified, and a simple scaling is proposed and shown to describe precisely the thinning down of the layers as a function of the initial tracer column’s horizontal width and the vertical shear of the azimuthal velocity. Nonlinear QG simulations were performed and analyzed for comparison with the work of Hua et al. A step-by-step interpretation of these results on the evolution of layering is proposed in the context of tracer stirring.


1982 ◽  
Vol 28 (4) ◽  
pp. 448-451 ◽  
Author(s):  
Garriet W. Smith ◽  
Steven S. Hayasaka

Nitrogenase activity (at in situ temperatures) associated with Zostera marina reflected the active growth periods of this plant in North Carolina coastal waters. During the plants most active growth period (late winter – spring) nitrogenase activity was primarily rhizospheric (8.47 μmol nitrogen fixed∙m−2∙day−1), while during its fall – early winter period it was primarily phyllospheric (8.03 μmol nitrogen fixed∙m−2∙day−1). No nitrogenase activity was detected during the warmer summer months when the plant is dormant. Phyllospheric nitrogenase activity (possibly the result of epiphytic heterocystic blue-green bacteria) was highest when plants were incubated aerobically in the presence of light.


2005 ◽  
Vol 33 (6) ◽  
pp. 1421-1422 ◽  
Author(s):  
J. Yang ◽  
S. Wongsa ◽  
V. Kadirkamanathan ◽  
S.A. Billings ◽  
P.C. Wright

Metabolic flux analysis using 13C-tracer experiments is an important tool in metabolic engineering since intracellular fluxes are non-measurable quantities in vivo. Current metabolic flux analysis approaches are fully based on stoichiometric constraints and carbon atom balances, where the over-determined system is iteratively solved by a parameter estimation approach. However, the unavoidable measurement noises involved in the fractional enrichment data obtained by 13C-enrichment experiment and the possible existence of unknown pathways prevent a simple parameter estimation method for intracellular flux quantification. The MCMC (Markov chain–Monte Carlo) method, which obtains intracellular flux distributions through delicately constructed Markov chains, is shown to be an effective approach for deep understanding of the intracellular metabolic network. Its application is illustrated through the simulation of an example metabolic network.


Metabolites ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 63 ◽  
Author(s):  
André Feith ◽  
Attila Teleki ◽  
Michaela Graf ◽  
Lorenzo Favilli ◽  
Ralf Takors

Dynamic 13C-tracer-based flux analyses of in vivo reaction networks still require a continuous development of advanced quantification methods applying state-of-the-art mass spectrometry platforms. Utilizing alkaline HILIC chromatography, we adapt strategies for a systematic quantification study in non- and 13C-labeled multicomponent endogenous Corynebacterium glutamicum extracts by LC-QTOF high resolution (HRMS) and LC-QQQ tandem mass spectrometry (MS/MS). Without prior derivatization, a representative cross-section of 17 central carbon and anabolic key intermediates were analyzed with high selectivity and sensitivity under optimized ESI-MS settings. In column detection limits for the absolute quantification range were between 6.8–304.7 (QQQ) and 28.7–881.5 fmol (QTOF) with comparable linearities (3–5 orders of magnitude) and enhanced precision using QQQ-MRM detection. Tailor-made preparations of uniformly (U)13C-labeled cultivation extracts for isotope dilution mass spectrometry enabled the accurate quantification in complex sample matrices and extended linearities without effect on method parameters. Furthermore, evaluation of metabolite-specific m+1-to-m+0 ratios (ISR1:0) in non-labeled extracts exhibited sufficient methodical spectral accuracies with mean deviations of 3.89 ± 3.54% (QTOF) and 4.01 ± 3.01% (QQQ). Based on the excellent HILIC performance, conformity analysis of time-resolved isotopic enrichments in 13C-tracer experiments revealed sufficient spectral accuracy for QQQ-SIM detection. However, only QTOF-HRMS ensures determination of the full isotopologue space in complex matrices without mass interferences.


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