scholarly journals Role of nuclear morphometry in cervicovaginal Papanicolaou smears and its utility in diagnosing cervical lesions

2020 ◽  
Vol 3 (1) ◽  
pp. 227-234
Author(s):  
Dr. Sindhu C ◽  
Dr. Shameem Shariff
Keyword(s):  
Cervical Lesions ◽  
Nuclear Morphometry ◽  
Papanicolaou Smears

E6 and E7 oncoproteins: Potential targets of cervical cancer

2020 ◽  
Vol 27 ◽  
Author(s):  
Ramarao Malla ◽  
Mohammad Amjad Kamal
Keyword(s):  
Cervical Cancer ◽  
Drug Resistance ◽  
Host Cell ◽  
Molecular Mechanisms ◽  
Immune Therapy ◽  
Cervical Lesions ◽  
Diagnostic Strategies ◽  
E6 And E7 Oncoproteins ◽  
E6 And E7

: Cervical cancer (CC) is the fourth leading cancer in women in the age group 15-44 globally. Experimental as well as epidemiological studies identified that type16 and 18 HPV cause 70% of precancerous cervical lesions as well as cervical cancer worldwide by bringing about genetic as well as epigenetic changes in the host genome. The insertion of the HPV genome triggers various defense mechanisms including the silencing of tumor suppressor genes as well as activation of oncogenes associated with cancer metastatic pathway. E6 and E7 are small oncoproteins consisting of 150 and 100 amino acids respectively. These oncoproteins affect the regulation of the host cell cycle by interfering with p53 and pRb. Further these oncoproteins adversely affect the normal functions of the host cell by binding to their signaling proteins. Recent studies demonstrated that E6 and E7 oncoproteins are potential targets for CC. Therefore, this review discusses the role of E6 and E7 oncoproteins in metastasis and drug resistance as well as their regulation, early oncogene mediated signaling pathways. This review also uncovers the recent updates on molecular mechanisms of E6 and E7 mediated phytotherapy, gene therapy, immune therapy, and vaccine strategies as well as diagnosis through precision testing. Therefore, understanding the potential role of E6/E7 in metastasis and drug resistance along with targeted treatment, vaccine, and precision diagnostic strategies could be useful for the prevention and treatment of cervical cancer.

scholarly journals Diagnostic Modalities of Precancerous and Cancerous Cervical Lesions with Special Emphasis on CD31 Angiogenesis Factor as a Marker

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Bandana Sharma ◽  
Neetu Singh ◽  
Neena Gupta ◽  
Pavika Lal ◽  
Shefali Pande ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Pap Smear ◽  
Histopathological Examination ◽  
Cervical Lesions ◽  
Invasive Group ◽  
Angiogenesis Factor ◽  
Medical College ◽  
Obstetrics And Gynaecology ◽  
Diagnostic Modalities

Objectives. To evaluate the role of angiogenesis tumor marker CD31 in the detection of precancerous and cancerous cervical lesions and to compare its efficacy with colposcopy and histopathology. Materials and Methods. 230 patients with a suspicious looking cervix and an abnormal Pap smear attending the Outpatient Department of Obstetrics and Gynaecology of GSVM Medical College were subjected to a colposcopic examination. 180 patients with suspected colposcopic findings were subjected to a colposcopic directed biopsy. Biopsy tissues were sent for histopathological examination out of which 50 biopsied samples were sent for immunostaining of CD-31. Statistical analysis was done. Results. Comparison of microvessel density (MVD) count by haematoxylin and eosin staining (HE) and immunostaining of CD31 in preinvasive group were and , respectively, and in invasive group were and , respectively, which showed that MVD was higher by CD31 both in preinvasive and invasive group, and it was statistically significant. Conclusion. Angiogenesis is a marker of tumor progression, and CD31 fixes up vessel better as compared to HE, so aggressiveness of the tumor can be better predicted by MVD-CD31 as compared to MVD-HE.

scholarly journals FEATURES OF DENTINE CHEMICAL COMPOSITION OF INTACT TEETH AND TEETH WITH WEDGE-SHAPED DEFECTS

2021 ◽  
Vol 74 (8) ◽  
pp. 1869-1875
Author(s):  
Svitlana P. Yarova ◽  
Iryna I. Zabolotna ◽  
Olena S. Genzytska ◽  
Andrii A. Komlev
Keyword(s):  
Chemical Composition ◽  
Chemical Elements ◽  
Cervical Region ◽  
Cervical Lesions ◽  
X Ray ◽  
Sodium Magnesium ◽  
Calcium Phosphorus ◽  
Cervical Area ◽  
Focused Beam

The aim: Is to define dentine chemical composition of intact teeth and those with wedge-shaped defects followed by the analysis of revealed differences. Materials and methods: Longitudinal sections of 22 clinically removed teeth (12 – clinically intact ones, 10 – with wedge-shaped defects) from both jaws were studied in patients aged between 25-54 years. JSM-6490 LV focused beam electron microscope (scanning) with system of energy-dispersive X-ray microanalysis INCA Penta FETх3 was used. The chemical composition of 148 dentine areas in the incisal region (tubercle), equator, cervical area has been determined as a percentage of the weight amounts of carbon, oxygen, calcium, phosphorus, sodium, magnesium, sulfur, chlorine, zinc, potassium, aluminum. Results: Dentine chemical composition of teeth with wedge-shaped defects differed from those of intact teeth by significantly lower content: sodium, chlorine and calcium – in the incisal region (tubercle); sodium, magnesium − at the equator; sodium, chlorine and calcium – in the cervical region (p≤0.05). In the sample groups with cervical pathology there was more sulfur and oxygen in the incisal region (tubercle), phosphorus and zinc – at the equator, carbon and potassium – in the cervical region (p≤0.05). Conclusions: Differences in the chemical composition of intact teeth and teeth with wedge-shaped defects, the presence of correlation between the studied chemical elements confirm the role of macro- and microelements in the pathogenesis of non-carious cervical lesions.

scholarly journals Relationship between the Cervical Microbiome, HIV Status, and Precancerous Lesions

mBio ◽  
2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Cameron Klein ◽  
Daniela Gonzalez ◽  
Kandali Samwel ◽  
Crispin Kahesa ◽  
Julius Mwaiselage ◽  
...  
Keyword(s):  
Hiv Positive ◽  
High Grade ◽  
Squamous Intraepithelial Lesions ◽  
Cervical Lesions ◽  
Cervical Cancers ◽  
Bacterial Microbiome ◽  
Bacterial Richness ◽  
Intraepithelial Lesions ◽  
Hiv Negative

ABSTRACT Nearly all cervical cancers are causally associated with human papillomavirus (HPV). The burden of HPV-associated dysplasias in sub-Saharan Africa is influenced by HIV. To investigate the role of the bacterial microbiome in cervical dysplasia, cytobrush samples were collected directly from cervical lesions of 144 Tanzanian women. The V4 hypervariable region of the 16S rRNA gene was amplified and deep sequenced. Alpha diversity metrics (Chao1, PD whole tree, and operational taxonomic unit [OTU] estimates) displayed significantly higher bacterial richness in HIV-positive patients (P = 0.01) than in HIV-negative patients. In HIV-positive patients, there was higher bacterial richness in patients with high-grade squamous intraepithelial lesions (HSIL) (P = 0.13) than those without lesions. The most abundant OTUs associated with high-grade squamous intraepithelial lesions were Mycoplasmatales, Pseudomonadales, and Staphylococcus. We suggest that a chronic mycoplasma infection of the cervix may contribute to HPV-dependent dysplasia by sustained inflammatory signals. IMPORTANCE HPV is known to be the causal agent in the majority of cervical cancers. However, the role of the cervical bacterial microbiome in cervical cancer is not clear. To investigate that possibility, we collected cervical cytobrush samples from 144 Tanzanian women and performed deep sequencing of bacterial 16S rRNA genes. We found that HIV-positive patients had greater bacterial richness (P = 0.01) than HIV-negative patients. We also observed that women with high-grade squamous intraepithelial lesions (HSIL) had greater cervical bacterial diversity than women with cytologically normal cervices. Data from our precise sampling of cervical lesions leads us to propose that Mycoplasma contributes to a cervical microbiome status that promotes HPV-related cervical lesions. These results suggest a greater influence of the bacterial microbiota on the outcome of HPV infection than previously thought.

Role of nuclear morphometry in diagnosis of salivary gland neoplasms

2020 ◽  
Vol 48 (12) ◽  
pp. 1181-1193
Author(s):  
Jai Kumar Chaurasia ◽  
Vikas Gupta ◽  
Vipul Mayank ◽  
Ishan Raj Tiwari ◽  
Deepti Joshi ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Salivary Gland Neoplasms ◽  
Nuclear Morphometry

The role of nuclear morphometry in prediction of prognosis for rhabdomyosarcoma in children

2004 ◽  
Vol 45 (4) ◽  
pp. 352-359 ◽  
Author(s):  
B Kazanowska ◽  
M Jelen ◽  
A Reich ◽  
W Tarnawski ◽  
A Chybicka
Keyword(s):  
Nuclear Morphometry ◽  
Prediction Of Prognosis

scholarly journals Efficacy of HPV Vaccination in Women Receiving LEEP for Cervical Dysplasia: A Single Institution’s Experience

Vaccines ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 45 ◽  
Author(s):  
Marco Petrillo ◽  
Margherita Dessole ◽  
Elettra Tinacci ◽  
Laura Saderi ◽  
Narcisa Muresu ◽  
...  
Keyword(s):  
Hpv Vaccine ◽  
Odds Ratio ◽  
Protective Factor ◽  
Hpv Vaccination ◽  
Univariate Analysis ◽  
Cervical Dysplasia ◽  
Protective Role ◽  
P Value ◽  
Cervical Lesions

The aim of this study was to assess the role of a human papilloma virus (HPV) vaccine after loop electrosurgical excision procedure (LEEP) in reducing recurrent cervical dysplasia. A series of 503 women with cervical dysplasia received LEEP between January 2012 and October 2018. Of these patients, 379 were treated between January 2012 and June 2017, thus ensuring an adequate follow-up time. We made three attempts to establish telephone contact with each patient; 77 women did not respond and were excluded from the final study population, which consisted of 302 patients. One hundred eighty-two (60.7%) women were vaccinated with an HPV vaccine within 4 weeks of LEEP and 103 (34.3%) were followed up with but not vaccinated. Recurrence of cervical dysplasia requiring a further LEEP procedure occurred in 30 (10.5%) women, of whom 17 (16.5%) were not vaccinated and 13 (7.1%) were vaccinated (p-value = 0.010). At univariate analysis, HPV vaccination after LEEP (odds ratio (OR) = 0.4, p-value = 0.020) emerged as an independent protective factor. Choosing as an outcome of the analysis only recurrence as severe cervical lesions, the protective role of HPV vaccination after LEEP was found to be much more relevant with an odds ratio of 0.2 (95% CI = 0.1–0.6, p-value = 0.02). Administration of an HPV vaccine after LEEP seems to reduce the risk of recurrence, thus suggesting that HPV vaccination has a role as an adjuvant treatment after LEEP.

Role of endocervical curettage in the diagnostic workup of preinvasive cervical lesions

1990 ◽  
Vol 31 (2) ◽  
pp. 153-156 ◽  
Author(s):  
F. Sesti ◽  
C. Farné ◽  
M. Mattei ◽  
E. Piccione
Keyword(s):  
Diagnostic Workup ◽  
Cervical Lesions

Matrix metalloproteinases-2, -3, and -9 secreted by explants of benign and malignant lesions of the uterine cervix

2004 ◽  
Vol 14 (2) ◽  
pp. 333-340 ◽  
Author(s):  
J. Argüello-Ramírez ◽  
E. PÉREZ-CÁRDENAS ◽  
R. Delgado-Chávez ◽  
G. Solorza-Luna ◽  
S. Villa-Treviño ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Poor Prognosis ◽  
Critical Role ◽  
Tumor Development ◽  
Cervical Lesions ◽  
Elevated Expression ◽  
Human Carcinomas ◽  
Malignant Lesions ◽  
Biological Development

Elevated expression of matrix metalloproteinases (MMPs) plays a critical role in extracellular matrix (EM) degradation in tumor development and prognosis of different human carcinomas. In cervical carcinoma (Ce Ca), the role of these proteinases in the biological development of this neoplasm is controversial. In the present study, we compared the secretion of MMP-2, MMP-3 and MMP-9 among 29 benign and premalignant cervical lesions (cervicitis and cervical intraepithelial neoplasias) and 46 tumoral explants of Ce Ca. The explants were cultured for 48 h. The gelatinases secreted into conditioned medium were revealed by zymography and quantified by densitometry. The results showed high levels of MMP-3 and MMP-9 in tumoral explants. In contrast, only the pro-MMP-2 was higher in benign cervical lesions, although both active and inactive MMP-2 species are associated with advanced clinical stages in tumoral samples, and only the secretion of MMP-3 was associated with unresponsiveness to radiotherapy. We can conclude that the expression of MMPs is related to the invasive process in Ce Ca and suggest that they may play a role in degradation of the EM during local invasion. In addition, MMP-3 secretion could be a marker of poor prognosis in Ce Ca.

Sign in / Sign up

Export Citation Format

Share Document