scholarly journals The Effect of Diabetes Mellitus on the Ventricular Epicardial Activation and Repolarization in Mice

2012 ◽  
pp. 363-370
Author(s):  
M. A. VAYKSHNORAYTE ◽  
A. O. OVECHKIN ◽  
J. E. AZAROV
Keyword(s):  
Diabetes Mellitus ◽  
Right Ventricular ◽  
Electrode Array ◽  
Ventricular Repolarization ◽  
Cardiac Repolarization ◽  
Diabetic Mice ◽  
Dispersion Of Repolarization ◽  
Electrocardiographic Markers ◽  
Potential Mapping ◽  
Activation Times

Cardiac repolarization is prolonged in diabetes mellitus (DM), however the distribution of repolarization durations in diabetic hearts is unknown. We estimated the ventricular repolarization pattern and its relation to the ECG phenomena in diabetic mice. Potential mapping was performed on the anterior ventricular surface in healthy (n=18) and alloxan-induced diabetic (n=12) mice with the 64-electrode array. Activation times, end of repolarization times, and activation-recovery intervals (ARIs) were recorded along with limb lead ECGs. ARIs were shorter in the left as compared to right ventricular leads (P<0.05). The global dispersion of repolarization, interventricular and apicobasal repolarization gradients were greater in DM than in healthy animals (P<0.03). The increased dispersion of repolarization and apicobasal repolarization gradient in DM correlated with the prolonged QTc and Tpeak-Tend intervals, respectively. The increased ventricular repolarization heterogeneity corresponded to the electrocardiographic markers was demonstrated in DM.

scholarly journals Global changes of ventricular repolarization in arrhythmogenic right ventricular dysplasia

2018 ◽  
Vol 5 (4) ◽  
pp. 35-43
Author(s):  
N. A. Arteyeva ◽  
I. A. Zemskov ◽  
T. A. Kurilenko ◽  
E. A. Parmon
Keyword(s):  
Right Ventricular ◽  
Arrhythmogenic Right Ventricular Dysplasia ◽  
Ventricular Repolarization ◽  
Lateral Part ◽  
Ecg Changes ◽  
T Wave ◽  
Wave Inversion ◽  
Dispersion Of Repolarization ◽  
Right Ventricular Dysplasia ◽  
The Right

Background.In arrhythmogenic right ventricular dysplasia (ARVD), repolarization disorders precede structural heart changes.Objective.The aim of this work was to evaluate repolarization changes in ARVD patients with various degree of electrocardiographic (ECG) changes.Design and methods. The standard ECG and vectorcardiograms (VCG) of patients with a confirmed ARVD diagnosis (N = 22, 13 men 51 ± 16 years and 9 women 50 ± 12 years), observed in the Medical Centre of V.A. Almazov, were analyzed. The degree of ECG changes was assessed by the number of precordial leads with negative T-wave, the global dispersion of repolarization — on the basis of the maximum interval between the peak and the end of T-wave, the spatial repolarization sequence — by T-vector direction, the action potential duration (APD) — on the basis of Q-Tpeak and Q-Tend intervals.Results.At no T-wave inversion in precordial leads, the latest repolarization took place in the posterior regions of ventricular base, at T-wave inversion only in the right precordial leads — in the lateral part of the right ventricle, at T-wave inversion in all precordial leads — in the antero-apical parts of the ventricles. Depending on the degree of ECG changes, the duration of repolarization increased in such a way that the shorter APD increased more than the longer ones; the global dispersion of repolarization was not increased.Conclusion.In ARVD, the increase of repolarization ECG changes is a result of inhomogeneous APD prolongation and the changes in ventricular repolarization sequence (up to it’s inversion), not accompanied with a growth of global dispersion of repolarization.

Hypoglycemic effects of Auricularia auricula polysaccharides on high fat diet and streptozotocin-induced diabetic mice using metabolomics analysis

2021 ◽  
Author(s):  
nannan liu ◽  
Xuefeng Chen ◽  
Juanna Song ◽  
Mengyin Chen ◽  
Pin Gong ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
High Fat Diet ◽  
Diabetic Mice ◽  
Male Mice ◽  
High Fat ◽  
Auricularia Auricula ◽  
Ultrahigh Performance Liquid Chromatography ◽  
Metabolomic Approach ◽  
Metabolomics Analysis

This study evaluated the hypoglycemic effect of Auricularia auricula polysaccharides (AAPs) on streptozotocin-induced type 2 diabetes mellitus (T2DM) male mice (C57BL/6J) using a metabolomic approach based on ultrahigh-performance liquid chromatography–Q...

scholarly journals Carbohydrate response element binding protein (ChREBP) modulates the inflammatory response of mesangial cells in response to glucose

2018 ◽  
Vol 38 (6) ◽  
Author(s):  
Yan Chen ◽  
Yan-Jun Wang ◽  
Ying Zhao ◽  
Jin-Cheng Wang
Keyword(s):  
Diabetes Mellitus ◽  
Inflammatory Response ◽  
Mesangial Cells ◽  
Binding Protein ◽  
The Body ◽  
Mrna Levels ◽  
Diabetic Mice ◽  
Response Element ◽  
Tnf Α ◽  
Element Binding Protein

Diabetic nephropathy (DN) is one of the most devastating complications of diabetes mellitus. Carbohydrate response element binding protein (ChREBP) is a basic helix–loop–helix leucine zipper transcription factor that primarily mediates glucose homeostasis in the body. The present study investigated the role of ChREBP in the pathogenesis of DN. The expression of ChREBP was detected in patients with type 2 diabetes mellitus (T2DM), diabetic mice, and mesangial cells. ELISA was used to measure cytokine production in mesangial cells. Flow cytometry analysis was performed to detect the apoptosis of mesangial cells in the presence of high glucose. The expression levels of ChREBP and several cytokines (TNF-α, IL-1β, and IL-6) were up-regulated in T2DM patients. The mRNA and protein levels of ChREBP were also significantly elevated in the kidneys of diabetic mice. Moreover, glucose treatment promoted mRNA levels of TNF-α, IL-1β, and IL-6 in mesangial cells. Glucose stimulation induced significant apoptosis of SV40 MES 13 cells. In addition, transfection with ChREBP siRNA significantly inhibited ChREBP expression. Consequently, the inflammatory responses and apoptosis were inhibited in SV40 MES 13 cells. These results demonstrated that ChREBP could mediate the inflammatory response and apoptosis of mesangial cells, suggesting that ChREBP may be involved in the pathogenesis of DN.

scholarly journals Atrial septal defect patients with greater shunts show susceptibility for ventricular arrhythmias

2020 ◽  
Vol 4 (Issue 3) ◽  
Author(s):  
Osman Can Yontar
Keyword(s):  
Ventricular Arrhythmia ◽  
Atrial Septal Defect ◽  
Septal Defect ◽  
Ventricular Repolarization ◽  
Systolic Pulmonary Artery Pressure ◽  
T Wave ◽  
Dispersion Of Repolarization ◽  
Increased Risk ◽  
Shunt Group ◽  
Ventricular Arrhythmogenesis

Objective: Ventricular arrhythmia episodes are not infrequent in patients with atrial septal defect (ASD). Disturbance in cardiac volume and pressures may lead to enlargement and fibrosis in heart. An interatrial volume displacement through septal defect, briefly interatrial shunt, is the major reason for this complication. Prolongation of the interval between the peak and end of the T wave (Tpeak to Tend, Tp-e) on the 12-lead electrocardiogram (ECG), is utilized as a marker of ventricular arrhythmogenesis during last years. The aim of this study was to assess if there is an impact of shunt ratio on ventricular repolarization in patients with ASD by using Tp-e interval, Tp-e/QT ratio, and Tp-e/QTc ratio. Methods: Patient records of Samsun Training and Research Hospital were retrospectively analyzed. Electrocardiograms of 133 patients, who were diagnosed as ASD between January 2016 and December 2019 were obtained and scanned. ECG intervals were measured. Shunt ratios, right ventricle diameters and volumes were also acquired. Patients were grouped into two by their calculated shunt ratio, ratio of ≥2.0 is accepted as a high shunt group and &lt;2.0 as a low shunt group. Results: Both groups’ baseline characteristics were similar. Right ventricular dimensions and systolic pulmonary artery pressure were higher in high shunt group. Furthermore, ASD patients with higher shunt ratio had significantly higher ECG measurements than controls, Tp-e: 103.0 (22.1) vs 76.2 (10.2); Tp-e/QT: 0.25 (0.03) vs 0.21 (0.02); Tp-e/QTc: 0.22 (0.03)  vs, 0.17 (0.02); for all p&lt;0.001). Of all ECG parameters; Tp-e (r=0.631, p&lt;0.001), Tp-e/QT (r=0.531, p&lt;0.001) and Tp-e/QTc (r=0.614, p&lt;0.001) had moderate correlation with shunt ratio. Conclusion: T wave peak-to-end interval is a measure of transmural dispersion of repolarization and accepted as a surrogate for increased ventricular arrhythmogenesis risk. Our findings show that ASD patients whose shunt ratio are ≥2.0 show increased risk for arrhythmias. Key words: atrial septal defect, electrocardiogram, ventricular arrhythmia, risk, ventricular repolarization

Effect of Crude Leaves Extract of Bersama Abyssinica on Blood Glucose Level and Serum Lipid Level of Streptozotocin-Induced Diabetic Mice: Evidence for In vivo Antidiabetic Activity

2021 ◽  
Vol 19 ◽  
Author(s):  
Zemene Demelash Kifle ◽  
Agumas Alemu Alehegn ◽  
Baye Yrga Adugna ◽  
Abebe Basazn Mekuria ◽  
Engidaw Fentahun Enyew
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Serum Lipid ◽  
Lipid Level ◽  
Serum Lipid Level ◽  
Diabetic Mice ◽  
Lipid Levels ◽  
Antidiabetic Effect ◽  
Bersama Abyssinica

Background: Diabetes mellitus is one of the major and common metabolic, and chronic disorders in the world. Several medicinal plants have been used globally for the management of diabetes mellitus. The current study aimed to study the anti-hyperglycemic and anti-hyperlipidemic effects of Bersama abyssinica. Methods: Antidiabetic effect of 80% methanolic crude extract of Bersama abyssinica was studied in repeated dose-treated STZ-induced diabetic mice model. The activities of Bersama abyssinica on serum lipid level and body weight were investigated on STZ-induced diabetic mice. Data were analyzed using one-way ANOVA and significant when the p-value was less than 0.05. Results: All doses of the crude 80% methanolic extract of Bersama abyssinica (100 mg/kg, 200 mg/kg, and 400 mg/kg) exhibited a noticeable BGL reduction when compared with baseline blood glucose level and diabetic control on the 7th and 14th days of administration. Moreover, the higher dose of the extract (at 400 mg/kg) significantly (p < 0.001, 54.3%) decreased the BGL in STZ-induced diabetic mice. The maximum decrement in fasting BGL was achieved at the 14th days: 34.92%, 41.10%, 54.30%, and 59.66%, respectively for BAC 100 mg/kg, BAC 200 mg/kg, BAC 400 mg/kg, and GLC 5 mg/kg treated groups. Bersama abyssinica also displayed a significant (p < 0.05) improvement of serum lipid levels and body weight. Conclusion: Bersama abyssinica crude extract exhibited a significant antidiabetic effect and prevented body weight loss in streptozotocin-induced diabetic mice. The finding also confirmed the valuable biochemical activity of Bersama abyssinica by improving serum lipid levels.

Sign in / Sign up

Export Citation Format

Share Document