SPR Biosensor for Quantification of Fetuin-A as a Promising Multibiomarker

Early diagnosis of ongoing malignant disease is crucial to improve survival rate and life quality of the patients and requires sensitive detection of specific biomarkers e.g. prostate-specific antigen (PSA), carcinoembryonic antigen (CEA), alpha-fetoprotein (AFP), etc. In spite of current technological advances, malignant diseases are still identified in rather late stages, which have detrimental effect on the prognosis and treatment of the disease. Here, we present a biosensor able to detect fetuin-A, a potential multibiomarker. The biosensing platform is based on polymer brush combining antifouling monomer units of N-(2-hydroxypropyl)methacrylamide (HPMA) and carboxybetaine methacrylamide (CBMAA), statistically copolymerized by surface-initiated atom transfer radical polymerization. The copolymer poly(HPMA-co-CBMAA) exhibits excellent non-fouling properties in the most relevant biological media (i.e. blood plasma) as well as antithrombogenic surface properties by preventing the adhesion of blood components (i.e. leukocytes; platelets; and erythrocytes). Moreover, the polymer brush can be easily functionalized with biorecognition elements maintaining high resistance to blood fouling and the binding capacity can be regulated by tuning the ratio between CBMAA and HPMA units. The superior antifouling properties of the copolymer even after biofunctionalization were exploited to fabricate a new plasmonic biosensor for the analysis of fetuin-A in real clinical blood plasma samples. The assay used in this work can be explored as label-free affinity biosensor for diagnostics of different biomarkers in real clinical plasma samples and to shift the early biomarker detection toward novel biosensor technologies allowing point of care analysis.

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A miniaturized electrochemical assay for homocysteine using screen-printed electrodes with cytochrome c anchored gold nanoparticles

The Analyst ◽

10.1039/c5an00752f ◽

2015 ◽

Vol 140 (17) ◽

pp. 6071-6078 ◽

Cited By ~ 13

Author(s):

Thangamuthu Madasamy ◽

Christian Santschi ◽

Olivier J. F. Martin

Keyword(s):

Gold Nanoparticles ◽

Cytochrome C ◽

Blood Plasma ◽

Point Of Care ◽

Plasma Samples ◽

Electrochemical Assay ◽

Screen Printed Electrodes ◽

Screen Printed

Electrochemical point-of-care analysis of homocysteine in a drop of the blood plasma samples.

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Detection of Coxiella burnetii Using Silicon Microring Resonator in Patient Blood Plasma

Micromachines ◽

10.3390/mi10070427 ◽

2019 ◽

Vol 10 (7) ◽

pp. 427 ◽

Cited By ~ 2

Author(s):

Bonhan Koo ◽

Choong Eun Jin ◽

Moonsuk Bae ◽

Yoon Ok Jang ◽

Ji Yeun Kim ◽

...

Keyword(s):

Infectious Disease ◽

Blood Plasma ◽

Coxiella Burnetii ◽

Q Fever ◽

Microring Resonator ◽

Clinical Samples ◽

Plasma Samples ◽

Label Free ◽

Highly Sensitive ◽

Low Sensitivity

Blood plasma from patients is a powerful resource for diagnosing infectious disease due to it having many genetic materials as well as being relatively easy to obtain. Thus, various biosensors have been investigated for diagnosing diseases in blood plasma. However, there are no optimized and validated sensors for clinical use due to the low sensitivity, complexity, and difficulties of removing the inhibitors from plasma samples. In this study, we described a silicon microring resonator sensor used to detect Coxiella burnetii from the blood plasma of Q-fever patients in a label-free, real-time manner. Q-fever is an infectious disease caused by Coxiella burnetii via direct contact or inhalation aerosols. We validated this biosensor in the blood plasma of 35 clinical samples (including 16 Q fever samples infected with Coxiella burnetii and 19 samples infected with other febrile diseases. The biosensors are capable of rapid (10 min), highly sensitive (87.5%), and specific (89.5%) detection in plasma samples compared to the use of the conventional method.

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Label-free Bacteria Quantification in Blood Plasma by a Bioprinted Microarray Based Interferometric Point-of-Care Device

ACS Sensors ◽

10.1021/acssensors.8b00789 ◽

2018 ◽

Vol 4 (1) ◽

pp. 52-60 ◽

Cited By ~ 17

Author(s):

Priyanka Dey ◽

Nuria Fabri-Faja ◽

Olalla Calvo-Lozano ◽

Roland A. Terborg ◽

Alexander Belushkin ◽

...

Keyword(s):

Blood Plasma ◽

Point Of Care ◽

Label Free ◽

In Blood Plasma

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Natural infections with different Plasmodium species induce antibodies reactive to a chimeric Plasmodium vivax recombinant protein

Malaria Journal ◽

10.1186/s12936-021-03626-0 ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Jessica N. McCaffery ◽

Balwan Singh ◽

Douglas Nace ◽

Alberto Moreno ◽

Venkatachalam Udhayakumar ◽

...

Keyword(s):

Plasmodium Vivax ◽

Malaria Elimination ◽

Malaria Incidence ◽

Binding Capacity ◽

Specific Antigen ◽

Plasmodium Species ◽

Population Level ◽

Antibody Detection ◽

Plasma Samples ◽

High Responders

Abstract Background As malaria incidence and transmission in a region decreases, it becomes increasingly difficult to identify areas of active transmission. Improved methods for identifying and monitoring foci of active malaria transmission are needed in areas of low parasite prevalence in order to achieve malaria elimination. Serological assays can provide population-level infection history to inform elimination campaigns. Methods A bead-based multiplex antibody detection assay was used to evaluate a chimeric Plasmodium vivax MSP1 protein (PvRMC-MSP1), designed to be broadly immunogenic for use in vaccine studies, to act as a pan-malaria serological tool based on its ability to capture IgG in plasma samples obtained from naturally exposed individuals. Samples from 236 US travellers with PCR confirmed infection status from all four major Plasmodium species infecting humans, Plasmodium falciparum (n = 181), Plasmodium vivax (n = 38), Plasmodium malariae (n = 4), and Plasmodium ovale (n = 13) were tested for IgG capture using PvRMC-MSP1 as well as the four recombinant MSP1-19 kD isoforms representative of these Plasmodium species. Results Regardless of infecting Plasmodium species, a large proportion of plasma samples from infected US travellers provided a high assay signal to the PvRMC-MSP1 chimeric protein, with 115 high responders out of 236 samples assessed (48.7%). When grouped by active infection, 38.7% P. falciparum-, 92.1% of P. vivax-, 75.0% P. malariae-, and 53.4% of P. ovale-infected individuals displayed high assay signals in response to PvRMC-MSP1. It was also determined that plasma from P. vivax-infected individuals produced increased assay signals in response to the PvRMC-MSP1 chimera as compared to the recombinant PvMSP1 for 89.5% (34 out of 38) of individuals. PvRMC-MSP1 also showed improved ability to capture IgG antibodies from P. falciparum-infected individuals when compared to the capture by recombinant PvMSP1, with high assay signals observed for 38.7% of P. falciparum-infected travellers in response to PvRMC-MSP1 IgG capture compared to just 1.1% who were high responders to capture by the recombinant PvMSP1 protein. Conclusions These results support further study of designed antigens as an approach for increasing sensitivity or broadening binding capacity to improve existing serological tools for determining population-level exposure to Plasmodium species. Including both broad-reacting and Plasmodium species-specific antigen-coated beads in an assay panel could provide a nuanced view of population-level exposure histories, an extensive IgG profile, and detailed seroestimates. A more sensitive serological tool for detection of P. vivax exposure would aid malaria elimination campaigns in co-endemic areas and regions where P. vivax is the dominant parasite.

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An Innovative Immunoanalysis Strategy towards Sensitive Recognition of PSA Biomarker in Human Plasma Samples Using Flexible and Portable Paper Based Biosensor: A New Platform towards POC Detection of Cancer Biomarkers Using Integration of Pen-on Paper Technology with Immunoassays Methods

ImmunoAnalysis ◽

10.34172/ia.2021.06 ◽

2021 ◽

Vol 1 (1) ◽

pp. 6-6

Author(s):

Fatemeh Farshchi ◽

Arezoo Saadati ◽

Houman Kholafazad kordasht ◽

Mohammad Hasanzadeh

Keyword(s):

Prostate Cancer ◽

Human Plasma ◽

Specific Antigen ◽

Cost Effective ◽

Plasma Samples ◽

Label Free ◽

Free Paper ◽

Detection Range ◽

Efficient Treatment ◽

Human Plasma Samples

Background: The prostate-specific antigen (PSA) is one of the best markers for detecting prostate cancer. Rapid and real time recognition of PSA biomarker could be helpful in the early diagnosis and efficient treatment of prostate cancer. One of the usual methods to identify this biomarker is ELISA, which has a picomolar detection range but requires specialized personnel and also this technique is time-consuming and expensive. Methods: Cost-effective POC devices are great solutions because they are very cost-effective, sensitive and simple, do not require expert operators and have a high response time in a short time. With that in mind, in this work, a novel and simple label-free paper-based electrochemical immunosensor were designed by using conductive Ag-ink and designed directly by pen on paper technique on the surface of photographic paper, which is a suitable substrate for antibody immobilization, for rapid detection of PSA. Results: Based on the obtained results, under the optimum conditions, the synthesized Ag ink has a great substrate for antibody (Ab) and antigen (Ag) immobilization. The linear range was from 0.001 to 30 μg/L and the obtained low limite of quantification (LLOQ) was 0.001μg/L. This immunosensor also tested in human plasma samples, which had good analytical power. Conclusion: The proposed paper-based immunoassay could be a hopefully new and cheap tool for the diagnosis of other biomarkers.

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Natural Infections With Different Plasmodium Species Induce Antibodies Reactive to A Chimeric Plasmodium Vivax Recombinant Protein

10.21203/rs.3.rs-70161/v1 ◽

2020 ◽

Author(s):

Jessica N McCaffery ◽

Balwan Singh ◽

Douglas Nace ◽

Alberto Moreno ◽

Venkatachalam Udhayakumar ◽

...

Keyword(s):

Malaria Elimination ◽

Malaria Incidence ◽

Binding Capacity ◽

Specific Antigen ◽

Plasmodium Species ◽

Chimeric Protein ◽

Population Level ◽

Parasite Prevalence ◽

Antibody Detection ◽

Plasma Samples

Abstract Background: As malaria incidence and transmission in a region decreases, it becomes increasingly difficult to identify areas of active transmission. Improved methods for identifying and monitoring foci of active malaria transmission are needed in areas of low parasite prevalence in order to achieve malaria elimination. Serological assays can provide population-level infection history to inform elimination campaigns.Methods: A bead-based multiplex antibody detection assay was used to evaluate a chimeric P. vivax MSP1 protein (PvRMC-MSP1), designed to be broadly immunogenic for use in vaccine studies, to act as a pan-malaria serological tool based on its ability to capture IgG in plasma samples obtained from naturally exposed individuals. Samples from 236 US travelers with PCR confirmed infection status from all four major Plasmodium species infecting humans, P. falciparum, P. vivax, P. malariae, and P. ovale were tested for IgG capture using PvRMC-MSP1 as well as the four recombinant MSP1-19 kD isoforms representative of these Plasmodium species.Results: Regardless of infecting Plasmodium species, a majority of plasma samples from infected US travelers provided a high assay signal to the PvRMC-MSP1 chimeric protein. Most individuals that responded to the PmMSP1 or PoMSP1 antigen also responded to PvRMC-MSP1, with very few individuals responding to PmMSP1 or PoMSP1 antigens alone. When grouped by active infection, we observed that plasma from P. vivax-infected individuals produced increased assay signals in response to the PvRMC-MSP1 chimera as compared to the recombinant PvMSP1 for 89.5% (34/38) of individuals. PvRMC-MSP1 also showed improved ability to capture IgG antibodies from P. falciparum-infected individuals when compared to the capture by recombinant PvMSP1. Conclusions: These results support further study of designed antigens as an approach for increasing sensitivity or broadening binding capacity to improve existing serological tools for determining population-level exposure to Plasmodium species. Including both broad-reacting and Plasmodium species-specific antigen-coated beads in an assay panel could provide a nuanced view of population-level exposure histories, an extensive IgG profile, and detailed seroestimates. A more sensitive serological tool for detection of P. vivax exposure would aid malaria elimination campaigns in co-endemic areas and regions where P. vivax is the dominant parasite.

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Electrochemical impedimetric detection of stroke biomarker NT-proBNP using disposable screen-printed gold electrodes

The EuroBiotech Journal ◽

10.24190/issn2564-615x/2017/02.09 ◽

2017 ◽

Vol 1 (2) ◽

pp. 165-176 ◽

Cited By ~ 5

Author(s):

Prima Dewi Sinawang ◽

Dorin Harpaz ◽

Luka Fajs ◽

Raymond Chee Seong Seet ◽

Alfred Iing Yoong Tok ◽

...

Keyword(s):

Natriuretic Peptide ◽

Point Of Care ◽

Cardioembolic Stroke ◽

Gold Electrodes ◽

Plasma Samples ◽

Label Free ◽

Point Of Care Test ◽

Novel Approach ◽

Set Up ◽

Screen Printed

Abstract Stroke is the second top leading cause of death globally. It is caused by an abrupt interruption of blood flow to the brain. In that course, brain natriuretic peptide (BNP) and its derivative N-terminal pro b-type natriuretic peptide (NT-proBNP), neurohormones produced mainly by the heart ventricles in response to excessive stretching of cardiomyocytes (heart muscle cells), are proven to be good biomarkers for heart failure diagnosis. Moreover, there is growing clinical interest of the use of NT-proBNP for stroke diagnosis and prognosis because it is significantly associated with cardioembolic stroke and secondary stroke reoccurrence, with sensitivity >90% and specificity >80%. However, in diagnostic settings, there is still a need to address the encountered analytical problems, particularly assay specificity and set up. In this study, a novel approach for NT-proBNP detection is demonstrated using an electrochemical immunoassay method. A label-free impedimetry immunosensor for stroke biomarker was developed using modified disposable screen-printed gold electrodes (SPGE) hosting specific anti-NT-proBNP capture antibody. The performance of our immunosensor was studied in the presence of NT-proBNP in both buffered and mock (porcine) plasma samples. A linear relation between the relative total resistance (ΔRtot) responses and the NT-proBNP concentrations in buffer was observed in a range from 0.1 to 5 ng mL-1 with a correlation coefficient (R2) of 0.94656. Overall, the biosensor has demonstrated the capability to quantitate NT-proBNP and differentiate such concentrations in a low concentration range, especially among 0, 0.1, 0.5, 1, and 3 ng mL-1 in plasma samples within 25 min. This range is valuable not only for classifying cardioembolic stroke (higher or equal to 0.5 ng mL-1), but also predicting the risk of secondary stroke reoccurrence (higher than 0.255 ng mL-1). Our biosensor has the potential to be used as an easy-to-use point-of-care test that is both accurate and affordable.

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PLASMA CORTISOL, CORTICOSTERONE AND NON-PROTEIN-BOUND CORTISOL IN EXTRACORPOREAL CIRCULATION

Acta Endocrinologica ◽

10.1530/acta.0.0690517 ◽

1972 ◽

Vol 69 (3) ◽

pp. 517-525 ◽

Cited By ~ 15

Author(s):

T. Uozumi ◽

H. Manabe ◽

Y. Kawashima ◽

Y. Hamanaka ◽

Y. Monden ◽

...

Keyword(s):

Cortisol Level ◽

Extracorporeal Circulation ◽

Plasma Cortisol ◽

Elevated Level ◽

Marked Decrease ◽

Binding Capacity ◽

Biologically Active ◽

Major Surgery ◽

Plasma Samples ◽

Effective Factor

ABSTRACT The response of plasma cortisol, corticosterone and non-protein-bound cortisol in the extracorporeal circulation was investigated in 14 patients. The pre-perfusion levels of plasma cortisol, corticosterone and non-protein-bound cortisol were significantly elevated. During and immediately after perfusion, the levels of cortisol and corticosterone were found to decrease significantly from the pre-perfusion levels, while the percentage of non-protein-bound cortisol was shown to increase significantly. This indicates a marked decrease in cortisol binding capacity of plasma during extracorporeal circulation. Moreover in 200 plasma samples, it was demonstrated that the cortisol level increased markedly and the cortisol binding capacity decreased slightly during and shortly after major surgery without perfusion. It is concluded that stressful situations in major surgery with or without perfusion are associated with markedly increased levels of biologically active non-protein-bound cortisol. The elevated level of non-protein-bound cortisol in surgery seems to be dependent on the increase in the level of plasma cortisol as well as on the decrease in the cortisol binding capacity of plasma. Although the increased plasma cortisol plays the most important role in surgery with no perfusion, the decreased cortisol binding capacity may be the more effective factor involved during perfusion.

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Electrochemical Immunosensors Based on Nanostructured Materials for Sensing of Prostate-Specific Antigen: A Review

Current Medicinal Chemistry ◽

10.2174/0929867328666201124151821 ◽

2020 ◽

Vol 28 ◽

Author(s):

Hayati Filik ◽

Asiye Aslıhan Avan ◽

Mustafa Özyürek

Keyword(s):

Prostate Specific Antigen ◽

Nanostructured Materials ◽

Specific Antigen ◽

Electrochemical Immunosensor ◽

Label Free ◽

Electrochemical Immunosensors ◽

Metal Metal ◽

Different Types ◽

Sandwich Type ◽

Carbon Based Nanomaterials

: The prostate-specific antigen (PSA) has been considered a crucial serological marker for distinguishing prostate based cancer. This surveys recent progress in the construction of nanomaterial-based electrochemical immunosensors for a PSA. This review (from 2015 to 2020) reports the latest progress in PSA sensing based on the employ of different types of nanostructured materials. The most popular used nanostructured materials are metal, metal oxide, carbon-based nanomaterials, and their hybrid architectures utilized for distinct amplification protocols. In this review, the electrochemical immunosensors for prostate-specific antigen sensing are classified into three categories such as sandwich type@labeled, label free@nonlabeled and aptamer-based electrochemical immunosensor.

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Label-Free Mass Spectrometry-Based Plasma Proteomics Identified LY6D, DSC3, CDSN, SERPINB12, and SLURP1,as Novel Protein Biomarkers For Pulmonary Tuberculosis

Current Proteomics ◽

10.2174/1570164617666191210105122 ◽

2019 ◽

Vol 17 ◽

Author(s):

Xiaoli Yu ◽

Lu Zhang ◽

Na Li ◽

Peng Hu ◽

Zhaoqin Zhu ◽

...

Keyword(s):

Pulmonary Tuberculosis ◽

Search Algorithm ◽

Differentially Expressed ◽

P Value ◽

Plasma Samples ◽

Label Free ◽

Protein Biomarkers ◽

Protein Database ◽

Leave One Out ◽

Potential Biomarkers

Aim: We aimed to identify new plasma biomarkers for the diagnosis of Pulmonary tuberculosis. Background: Tuberculosis is an ancient infectious disease that remains one of the major global health problems. Until now, effective, convenient, and affordable methods for diagnosis of Pulmonary tuberculosis were still lacked. Objective: This study focused on construct a label-free LC-MS/MS based comparative proteomics between six tuberculosis patients and six healthy controls to identify differentially expressed proteins (DEPs) in plasma. Method: To reduce the influences of high-abundant proteins, albumin and globulin were removed from plasma samples using affinity gels. Then DEPs from the plasma samples were identified using a label-free Quadrupole-Orbitrap LC-MS/MS system. The results were analyzed by the protein database search algorithm SEQUEST-HT to identify mass spectra to peptides. The predictive abilities of combinations of host markers were investigated by general discriminant analysis (GDA), with leave-one-out cross-validation. Results: A total of 572 proteins were identified and 549 proteins were quantified. The threshold for differentially expressed protein was set as adjusted p-value < 0.05 and fold change ≥1.5 or ≤0.6667, 32 DEPs were found. ClusterVis, TBtools, and STRING were used to find new potential biomarkers of PTB. Six proteins, LY6D, DSC3, CDSN, FABP5, SERPINB12, and SLURP1, which performed well in the LOOCV method validation, were termed as potential biomarkers. The percentage of cross-validated grouped cases correctly classified and original grouped cases correctly classified is greater than or equal to 91.7%. Conclusion: We successfully identified five candidate biomarkers for immunodiagnosis of PTB in plasma, LY6D, DSC3, CDSN, SERPINB12, and SLURP1. Our work supported this group of proteins as potential biomarkers for pulmonary tuberculosis, and be worthy of further validation.

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