scholarly journals Investigating TRSV Phytoalexin Infusions as a Novel Treatment for Alzheimer’s Disease Using ApoE4 Plasmid Transfections in Neuro2a Cell Complexes

2020 ◽  
Vol 7 (5) ◽  
Author(s):  
Om Gandhi
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Neuro2a Cell ◽  
Cell Complexes ◽  
Novel Treatment

scholarly journals Investigating TRSV Phytoalexin Infusions as a Novel Treatment for Alzheimer’s Disease Using ApoE4 Plasmid Transfections in Neuro2a Cell Complexes

2020 ◽  
Author(s):  
Om Gandhi
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Amyloid Plaques ◽  
Grape Seed ◽  
Neuro2a Cell ◽  
Pass Filter ◽  
Low Pass Filter ◽  
Cell Complexes ◽  
Novel Treatment ◽  
Neuro2a Cells

AbstractBecause the accumulation of amyloid plaques plays a central role in the pathogenesis of Alzheimer’s disease (AD) and is responsible for many of its neurodegenerative effects, this project aims to evaluate the effectiveness of TRSV phytoalexin infusions as a novel treatment for the disease by comparing three separate agents and determining its impact on the accumulation of amyloid plaques in mouse Neuro2a cells. The experimental model is as follows: Neuro2a cells were first thawed using incubation and centrifugation techniques. The subculturing protocol was then performed to maximize cellular viability using DPBS and TrypLE dissociation reagents. The cells were transfected with pCMV4-ApoE4 bacterial plasmid using Opti-MEM medium and Lipofectamine LTX while also being treated with phytoalexin agents. Bradford’s assay was performed on the samples using CBB G-250 reagent, and they were run through a spectrophotometer and a Python low-pass filter to generate readings. The experimental data showed that natural grape seed phytoalexin extract was the most effective treating agent, followed by curcumin extract and synthetic RDS phytoalexin. All treating agents lessened the accumulation of the amyloid plaques significantly, supporting the novel infusion protocol as a treatment for AD.

scholarly journals Nutraceutical Approaches of Autophagy and Neuroinflammation in Alzheimer’s Disease: A Systematic Review

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 6018
Author(s):  
Reinhard Gruendler ◽  
Berit Hippe ◽  
Vesna Sendula Jengic ◽  
Borut Peterlin ◽  
Alexander G. Haslberger
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Preventive Measures ◽  
Memory Loss ◽  
Epigallocatechin Gallate ◽  
Clinical Impact ◽  
Food Sources ◽  
Severe Dementia ◽  
Novel Treatment ◽  
Positive Outlook

Aging and the emergence of age-associated illnesses are one of the major challenges of our present society. Alzheimer’s disease (AD) is closely associated with aging and is defined by increasing memory loss and severe dementia. Currently, there are no therapy options available that halt AD progression. This work investigates three hallmarks of the disease (autophagy, neuroinflammation, and senescence) and systematically analyzes if there is a beneficial effect from three substances derived from food sources, the so called “nutraceuticals” epigallocatechin gallate, fisetin, and spermidine, on these hallmarks. The results imply a positive outlook for the reviewed substances to qualify as a novel treatment option for AD. A combination of nutraceutical substances and other preventive measures could have significant clinical impact in a multi-layered therapy approach to counter AD.

scholarly journals The role of epigenetic modifications in Alzheimer’s disease

2020 ◽  
Vol 9 (1) ◽  
pp. 294
Author(s):  
Shierly . ◽  
Chandra Wirawan
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Clinical Manifestations ◽  
Epigenetic Modifications ◽  
Novel Treatment ◽  
Amyloid Aβ ◽  
Memory Deterioration ◽  
Primary Risk Factor ◽  
Cognitive Disturbance

Aging is the primary risk factor for various neurodegenerative diseases, including Alzheimer’s disease (AD), which is the most frequent form of Dementia. AD is progressive neurodegenerative disease with abnormal protein production, inflammation and memory deterioration. The main clinical manifestations of this illness are cognitive disturbance and memory deficit. Abnormal of beta-amyloid (Aβ), neurofibrillary tangles (NFTs) and tau deposition are the most common findings pathology in this disease. Recent studies indicate that epigenetic modifications strongly correlate in developing these pathology and disease progression. The hallmarks of epigenetic modifications are DNA (deoxyribonucleic acid) methylation, histone modifications, chromatin remodeling and ncRNA (non-coding ribonucleic acid) expressions. This review aims to explain the potential mechanisms of epigenetic modifications associate with this disease. The general conclusion of this review is that epigenetic modifications play an ultimate role in AD and there are potential biomarkers of AD and future novel treatment of AD based on epigenetics.

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