In vitro Researches into the Biocompatibility of Titanium Alloys with a Modifi ed Surface

This work presents the results of a comparative in vitro study into the biocompatibility of titanium samples, the surface of which was pre-treated using various methods: milling, machining with acid etching and irradiation with a powerful ion beam (PIB) for creating a relief with the required roughness and the shape of asperities. A study of the effectiveness of attachment and growth of Balb/NIH 3T3 fi broblasts on titanium products with various types of surface treatment demonstrated that irradiation of a titanium surface with a powerful ion beam (PIB) leads to its active colonization with cells having a fl attened and stellate shape, which confi rms their active growth. An assessment of migration of metal impurities from the titanium alloy VT 1-0 was evaluated using the x-ray fl uorescence method

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Comparative Evaluation of Microroughness created on Titanium Alloy for use in Dental Implants subjected to Two Different Acid Etching Techniques: An in vitro Study

Journal of Contemporary Dentistry ◽

10.5005/jp-journals-10031-1134 ◽

2016 ◽

Vol 6 (1) ◽

pp. 1-8

Author(s):

Vishwas Madaan ◽

Ashvini M Padhye ◽

Manjeet Mapara

Keyword(s):

Titanium Alloy ◽

Dental Implants ◽

Comparative Evaluation ◽

In Vitro Study ◽

Vitro Study ◽

Acid Etching ◽

Titanium Plate ◽

Average Roughness ◽

Etching Technique

ABSTRACT Aim The aim of this study was to comparatively evaluate the microroughness created on titanium alloy for use in dental implants subjected to two different acid etching techniques. Materials and Methods Commercially available grade 5 pure titanium plates were machine prepared into 26 plates measuring 3 × 1 cm × 5 mm for acid etching with hydrofluoric acid (HF) and dual acid etching technique using sulfuric acid (H2SO4) followed by hydrochloric acid (HCl). Twenty-five plates were divided into four groups based on the duration and sequence of acid etching. Upon completion of the acid etching procedure, the titanium plates were assessed for their surface characteristics by a surface profilometer. The average roughness parameters values Ra, Rq, Rz obtained for each titanium plate were compared against each other and with unetched titanium plate. Results The average roughness value Ra obtained was 0.480 μm for untreated surface and 3.65 μm maximum for the titanium plate etched for 72 hours in H2SO4 and 48 hours in HCl, which is about seven times the value of surface roughness on the unetched plates. The roughness values obtained after acid etching with HF for any duration were nonsignificant compared with the unetched plates. Conclusion The dual acid etching technique seems to be a simple method to develop a titanium implant surface, though evaluation of the biological response to this surface is necessary. Clinical significance The present study showed that by optimizing the parameters of acid etching, a rough titanium surface can be obtained similar to the various implant surfaces available commercially. How to cite this article Madaan VU, Mapara M, Padhye AM. Comparative Evaluation of Microroughness created on Titanium Alloy for use in Dental Implants subjected to Two Different Acid Etching Techniques: An in vitro Study. J Contemp Dent 2016;6(1):1-8.

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Revealing Inflammatory Indications Induced by Titanium Alloy Wear Debris in Periprosthetic Tissue by Label-Free Correlative High-Resolution Ion, Electron and Optical Microspectroscopy

Materials ◽

10.3390/ma14113048 ◽

2021 ◽

Vol 14 (11) ◽

pp. 3048

Author(s):

Rok Podlipec ◽

Esther Punzón-Quijorna ◽

Luka Pirker ◽

Mitja Kelemen ◽

Primož Vavpetič ◽

...

Keyword(s):

Titanium Alloy ◽

High Resolution ◽

Wear Debris ◽

Ion Beam ◽

Quantitative Measure ◽

Label Free ◽

Periprosthetic Tissue ◽

Correlative Microscopy ◽

X Ray ◽

Tissue Reactions

The metallic-associated adverse local tissue reactions (ALTR) and events accompanying worn-broken implant materials are still poorly understood on the subcellular and molecular level. Current immunohistochemical techniques lack spatial resolution and chemical sensitivity to investigate causal relations between material and biological response on submicron and even nanoscale. In our study, new insights of titanium alloy debris-tissue interaction were revealed by the implementation of label-free high-resolution correlative microscopy approaches. We have successfully characterized its chemical and biological impact on the periprosthetic tissue obtained at revision surgery of a fractured titanium-alloy modular neck of a patient with hip osteoarthritis. We applied a combination of photon, electron and ion beam micro-spectroscopy techniques, including hybrid optical fluorescence and reflectance micro-spectroscopy, scanning electron microscopy (SEM), Energy-dispersive X-ray Spectroscopy (EDS), helium ion microscopy (HIM) and micro-particle-induced X-ray emission (micro-PIXE). Micron-sized wear debris were found as the main cause of the tissue oxidative stress exhibited through lipopigments accumulation in the nearby lysosome. This may explain the indications of chronic inflammation from prior histologic examination. Furthermore, insights on extensive fretting and corrosion of the debris on nm scale and a quantitative measure of significant Al and V release into the tissue together with hydroxyapatite-like layer formation particularly bound to the regions with the highest Al content were revealed. The functional and structural information obtained at molecular and subcellular level contributes to a better understanding of the macroscopic inflammatory processes observed in the tissue level. The established label-free correlative microscopy approach can efficiently be adopted to study any other clinical cases related to ALTR.

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Quantitative evaluation of microleakage in class V cavities using one-bottle and self-etching adhesive systems

Brazilian Oral Research ◽

10.1590/s1806-83242004000300014 ◽

2004 ◽

Vol 18 (3) ◽

pp. 253-259 ◽

Cited By ~ 2

Author(s):

Fabiana Mantovani Gomes França ◽

Flávio Henrique Baggio Aguiar ◽

Alex José Souza dos Santos ◽

José Roberto Lovadino

Keyword(s):

Methylene Blue ◽

In Vitro Study ◽

Adhesive System ◽

Acid Etching ◽

Single Bond ◽

Adhesive Systems ◽

Class V ◽

Bonding System ◽

Root Dentin

The aim of this in vitro study was to evaluate quantitatively the microleakage in class V cavities restored with one-bottle and self-etching adhesive systems with and without previous acid etching. Two one-bottle adhesive systems (Single Bond and Prime & Bond 2.1) and one self-etching adhesive system (Clearfil Mega Bond) were used in this study. One hundred and twenty sound human premolar teeth were randomly divided into 6 groups, and 20 class V restorations were prepared in the root dentin to test each bonding system. Each bonding system was used with and without acid etching. Specimens were prepared, dyed with 2% methylene blue, sectioned, triturated, and evaluated with an absorbance spectrophotometer test in order to quantify the infiltrated dye. Results were statistically evaluated by ANOVA and Tukey-Kramer test. No statistically significant differences were found among the adhesive systems when no etching agent was used. However, the Single Bond adhesive system showed statistically significant lower microleakage means than Clearfil Mega Bond and Prime & Bond 2.1 when 37% phosphoric acid was used. Single Bond and Clearfil Mega Bond adhesive systems presented similar behavior when the manufacturers' instructions were followed.

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