scholarly journals Determination of Pesticide Residues in Strawberry (Fragaria ananassa Duch.) from Upper Punjab Districts of Pakistan

2021 ◽  
Vol 3 (1) ◽  
pp. 39-47
Author(s):  
Muhammad Adnan Rafique ◽  
Abdul Qadir ◽  
Muhammad Ali ◽  
Adnan Noor Shah ◽  
Muhammad Ameen ◽  
...  
Keyword(s):  
Pesticide Residues ◽  
Present Experiment ◽  
Limit Of Detection ◽  
Fragaria Ananassa ◽  
Pesticides Residues ◽  
Detection And Quantification

Strawberry (Fragaria ananassa) is grown from mid-October to the end of April in Lahore, Sheikhupura, Sialkot and Gujranwala Districts of the Punjab. A huge amounts of pesticides especially fungicides are sprayed on it indiscriminately to protect it from different diseases and fungal attacks. Present experiment was planned for determination of pesticide residues in strawberry. For this purpose, 40 samples of strawberry were collected from Sheikhupura, Lahore, Sialkot, and Gujranwala districts of Punjab, Pakistan for determination of residues of Malathion, Chlorpyrifos, Profenofos, Atrazine, Carbofuron, Tricyclazole, Difenoconazole, Azoxystrobin, Tebuconazole and Cypermethrin in strawberry. The accuracy, precision, specificity, linearity, limit of detection and quantification were measured. The recovery ranged from 89.3 % to 108% with RSD ranging from 1.66 % to 14.2 %. The linearity showed reliable range (0.995–0.999). The limit of detection ranged from 0.006 to 0.04 μg g-1. Data showed that all the samples contain multi-residues of different pesticides. Among these 40 samples pesticide residues were most often found. All the samples contained multiresidues of different pesticides. The percentage of samples having pesticides residues are , Carbofuron (50 %), Malathion (37.50 %), Profenofos (32.50 %), Chlorpyrifos(25 %), Cypermethrin (20 %), Difenoconazole  in (47.50 %), Tricyclazol in ( 52.50 %), Tebuconazole in (50.00 %), and Azoxystrobin in (20 %) of the samples analyzed.

scholarly journals Pesticide Residues in Pineapple Juice in Abidjan, Cote D’ivoire

2020 ◽  
pp. 91-97
Author(s):  
AKE ASSI Yolande Epouse DATTE ◽  
GNONSORO Urbain Paul ◽  
KOUAKOU Konan Jean-Marie ◽  
KPAN KPAN Kouakou Gains ◽  
BIÉGO Godi Henri ◽  
...  
Keyword(s):  
Cote D'ivoire ◽  
Pesticide Residues ◽  
High Performance ◽  
Côte D’Ivoire ◽  
Limit Of Detection ◽  
Cote D’Ivoire ◽  
Pesticides Residues ◽  
Pineapple Juice ◽  
Côte D'ivoire

The determination of pesticides in food products is an essential issue to guarantee food safety and minimize health risks of consumers. In this study, 30 pineapple juice samples were collected randomly from 30 seller through the city of Abidjan (Côte d’Ivoire) and analyzed for the determination of pesticide residues. The aim of the work was to quantify 30 commonly used pesticides (Metolachlor, Chlorpropham, Parathion-methyl, Chlorfenvinphos, Vinclozolin, Parathion-ethyl, Fenuron, Aldicarb, Metoxuron, Monuron, Methabenzthiazuron, Chlortoluron, Monolinuron, Isoproturon, Diuron, Metobromuron, Metazachlor, Buturon, Linuron, Prometryn, Terbutryn, Desisopropylatrazine, Desethylatrazine, Simazine, Cyanazine, Atrazine, Propazine, Terbuthylazine, Metamitron, Crimidine and Metolachlor) in real samples of pineapple juice. The method used for the determination of these analytes in the complex matrices was high-performance liquid chromatography with UV/Visible detector. Results obtained indicate that 30% of the investigated pineapple juices samples are free of pesticides residues or have a level below Limit Of Detection......

Determination of multiple organochlorine pesticide residues in shrimp using modified QuEChERS extraction and gas chromatography

2018 ◽  
Vol 16 (1) ◽  
pp. 81-93
Author(s):  
MDH Prodhan ◽  
SN Alam
Keyword(s):  
Gas Chromatography ◽  
Organochlorine Pesticide ◽  
Pesticide Residues ◽  
Limit Of Detection ◽  
Residue Analysis ◽  
Limit Of Quantification ◽  
Modified Quechers ◽  
Endosulfan Sulphate ◽  
Organochlorine Pesticide Residues

Determination of organochlorine pesticide residues in shrimp is very important to ensure the consumer’s safety and to fulfill the importer’s demand. Therefore, a simple and efficient multiple organochlorine pesticide residues analytical method using quick, easy, cheap, effective, rugged and safe (QuEChERS) extraction technique and Gas Chromatography coupled with Electron Capture Detector (ECD) has been developed and validated for the determination of 19 organochlorine pesticides (α- BHC, δ- BHC, β- BHC, γ- BHC, Heptachlor, Aldrin, Heptachlor Epoxide, γ- Chlordane, α- Chlordane, α- Endosulfan, 4,4 DDE, Dieldrin, Endrin, 4,4 DDD, β- Endosulfan, 4,4 DDT, Endosulfan sulphate, Methoxychlor, and Endrin Ketone) in shrimp. The method was validated by evaluating the accuracy, precision, linearity, limit of detection (LOD) and limit of quantification (LOQ). The average recoveries of the selected pesticides ranged from 84% to 106% with RSDr ≤ 14% in four fortification levels of 0.05, 0.1, 0.2 and 0.3 mg kg-1. The linearity was ≥ 0.996 for all of the selected pesticides with matrix matched calibration standards. The LOD ranged from 0.003 to 0.009 mg kg-1 and the LOQ was 0.05 mg kg-1. This method was applied successfully for the residue analysis of 40 shrimp samples collected from different regions in Bangladesh.SAARC J. Agri., 16(1): 81-93 (2018)

scholarly journals Development and method validation for determination of 54 pesticides in Okra by LC-MS/MS analysis

2020 ◽  
Vol 11 (1) ◽  
pp. 985-992
Author(s):  
Hymavati Muppalla ◽  
Kiranmayi Peddi
Keyword(s):  
European Union ◽  
Pesticide Residues ◽  
High Performance ◽  
Limit Of Detection ◽  
Extraction Procedure ◽  
Mass Spectrometry Analysis ◽  
Limit Of Quantification ◽  
Spectrometry Analysis ◽  
Tandem Mass Spectrometry Analysis

The presence of pesticide residues in primary and derived agricultural products raises serious health concerns for consumers across the globe. The aim of the present study was to assess the level of pesticide residues in Okra in India. A multi-residue method for the quantification of fifty-four pesticides in okra is described in this work. The present study employed a modified quick, easy cheap, effective rugged and safe (QuEChERS) extraction procedure followed by UHPLC-MS/MS (Ultra-High-Performance Liquid Chromatography coupled to Tandem Mass Spectrometry) analysis. Validation of the method was according to the guidelines given by European Union SANCO/12571/2013. The levels of validation were 10.0, 50.0 and 100 µg kg-1. The following parameters such as linearity, the limit of detection (LOD) (nearer to 0.005 mg kg-1) and limit of quantification (LOQ) (nearer to 0.01 mg kg-1) were set to be acceptable. The trueness of the method for 54 pesticides in all Okra commodities was between 80-110% with satisfactory repeatability and within-run reproducibility except for the pesticide residues such as Thiamethoxam and Fenamidone. The measurement of uncertainty for each of the pesticide was below 50% and was estimated to be in the range of 5.37% - 10.71%, which meets the criteria established in the SANCO/12571/2013 document (European Union, 2013). This method is concluded to be applicable for the determination of pesticide residues in Okra.

scholarly journals Sensitive Spectrophotometric Determinations of Paracetamol and Protriptyline HCl Using 3-Chloro-7-hydroxy-4-methyl-2H-chromen-2-one

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Kumble Divya ◽  
Badiadka Narayana ◽  
Majal Sapnakumari
Keyword(s):  
Charge Transfer ◽  
Spectrophotometric Method ◽  
Charge Transfer Complex ◽  
Limit Of Detection ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Pharmaceutical Samples ◽  
Chromogenic Reagent ◽  
Detection And Quantification

A new spectrophotometric method is developed for the determination of Paracetamol (PCT) and protriptyline HCl (PTP) in pure forms and in pharmaceutical formulations. The experiment involves the use of 3-chloro-7-hydroxy-4-methyl-2H-chromen-2-one as a novel chromogenic reagent for the determination of PCT and PTP. The method is based on the formation of charge transfer complex between the drugs and chromogenic reagent. Beer's law is obeyed in the concentration ranges 10.00–60.00 µg mL−1 for PCT at 545 nm and 40.00–160.00 µg mL−1 for PTP at 468 nm. The molar absorptivity, Sandell, sensitivity, and limit of detection and quantification are also calculated. The method has been successfully applied for the determination of both PCT and PTP in pharmaceutical samples with acceptable results.

Determination of Phenicols Using a Fast and Reliable HPLC Method Developed on Hypercarb Stationary Phase

2017 ◽  
Vol 68 (3) ◽  
pp. 545-548
Author(s):  
Georgeta Simona Stan ◽  
Florentina Moldovanu ◽  
Irinel Adriana Badea
Keyword(s):  
Stationary Phase ◽  
Mobile Phase ◽  
Reliable Method ◽  
Limit Of Detection ◽  
Hplc Method ◽  
Detection Capability ◽  
Milk Samples ◽  
Precision And Accuracy ◽  
Detection And Quantification

Hypercarb porous graphitic stationary phase was used to develop a fast and reliable method for simultaneous determination of chloramphenicol, thiamphenicol and florfenicol. The separation was achieved in 7 min elution being made isocratic using water modified with acetonitrile as mobile phase. The method was fully validated and showed good linearity, precision and accuracy. Limit of detection and quantification together with decision limit and detection capability were established. This method was applied for analysis of milk samples.

scholarly journals Determination of Pesticide Residuals in Soil and Tomato Fruits from Two Tomato Production Areas in northern Ghana

2020 ◽  
Vol 6 (2) ◽  
pp. 37-44
Author(s):  
Linda Dari ◽  
Ahmad ADDO ◽  
Komla Agbeko DZISI
Keyword(s):  
Pesticide Residues ◽  
High Performance ◽  
Tomato Fruit ◽  
Northern Region ◽  
Efficient Estimation ◽  
Northern Ghana ◽  
Tomato Fruits ◽  
Tomato Production ◽  
Pesticides Residues

Tomato fruit (Lycopersicon esculentum Mill) is an important vegetable commodity in Ghana, as it is consumed daily in many households either heat-treated or without any form of heat treatments. Tomato production is a major source of income for many smallholder producers in Northern Ghana especially through dry season farming when the major supply of tomatoes from Southern Ghana is exhausted. Research conducted for the past decade confirmed the presence of pesticide residues in fruits and vegetables such as cabbage, onion, cucumber, lettuce, tomatoes, okra and pepper. The objective of this study was to identify and estimate pesticide residual levels in the soil and tomato fruits in comparison with the maximum allowable residual limits. The research was carried out in two production communities namely Doba where the “Burkina” variety is mostly grown in the Kassena Nankana East District of the Upper East Region and Bunglung where the “Wosowoso” variety is cultivated in the Savelugu/Nanton Municipality of Northern Region. Soil samples were collected for residue determination before transplanting of tomato seedlings. Matured and ripe tomato fruits were also collected for the determination of the presence and amount of pesticide residues. All soil and plant samples were analysed using high performance liquid chromatography to determine the presence of twenty-four organochlorines and thirteen organophosphate pesticide residues. From the analysis, pesticide residues were present in different variations which ranged from 0.002 – 0.033 and 0.003 – 0.022 (soils) and 0.330 – 1.187 and 0.002 – 0.088 (fruits) for organochlorines and organophosphates respectively for both communities. Levels of pesticide residues were generally above the acceptable maximum residue limits as farmer practices produced fruits with more pesticide residues since the land areas could have been predisposed with residues from previous seasons for other food crops, which could be translocated into the tomato plant and through into the fruits. The presence of pesticide residues could also be attributed to the influence of run-off and drift from other cultivated lands. For effective determination of pesticides residues in the tomato plants, it is essential to use uncontaminated soil and water to facilitate the efficient estimation of pesticide residues in tomatoes and plants in general.

scholarly journals Validated UV-Vis Spectrophotometric Determination of Andrographolide in Herbal Nano-Preparation

2019 ◽  
Vol 31 (9) ◽  
pp. 1985-1988
Author(s):  
Indah Hairunisa ◽  
Muhammad Da'i ◽  
Erindyah Retno Wikantyasning ◽  
Andhika Rizky Gilang Mahaputra ◽  
Normaidah Normaidah ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Picric Acid ◽  
Limit Of Quantification ◽  
Intermediate Precision ◽  
Herbal Products ◽  
Relative Standard ◽  
Acid Reagent ◽  
Interday Precision ◽  
Detection And Quantification

Determination of major bioactive compounds in polyphyto-formulation is important for production of standardized herbal products. A fast, simple and inexpensive method for detection and quantification of andrographolide concentration in nanoemulsion preparations containing a combination of Andrographis paniculata (Burm f.) Ness. and Phyllanthus niruri L. has been developed. Detection and quantification were carried out using UV-vis spectrophotometry analysis with picric acid reagent and NaOH (8:2) in methanol solvents, read at maximum wavelength 479 nm with 22 min of incubation time. Validation was done by determine the parameters such as linearity, intra and interday precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ). The results obtained showed linearity with r = 0.9945 (y = 0.0109x − 0.2066) in the range of 30-80 μg/mL. The accuracy (recovery) varied in the range of 97.15 to 104.42 %. Percentage of relative standard deviation (% RSD) for precision and intermediate precision value were 3.23 and 3.02 % with LOD value 211 μg/mL and the LOQ 705 μg/mL. As a conclusion, this method is suitable to detect andrographolide content in herbal nano-preparation.

scholarly journals DETERMINAÇÃO DE RESÍDUOS DE AGROTÓXICOS ORGANOCLORADOS EM HORTALIÇAS

2002 ◽  
Vol 12 ◽  
Author(s):  
MARIA TERESA PLATA OVIEDO ◽  
MARIA CECÍLIA DE FIGUEIREDO TOLEDO ◽  
EDUARDO VICENTE
Keyword(s):  
Gas Chromatography ◽  
Pesticide Residues ◽  
Detection Limits ◽  
Heptachlor Epoxide ◽  
Endosulfan Sulfate ◽  
Average Recovery ◽  
Pesticides Residues ◽  
Organochlorine Pesticide Residues ◽  
The University

Foram analisados resíduos de Clorotalonil, Aldrin, Dieldrin, Heptacloro, Heptacloro epóxido, Endosulfan alfa, Endosulfan beta e Sulfato de Endosulfan nas seguintes hortaliças: alface, acelga, chicória, repolho, tomate, chuchu, batata, cenoura, mandioca e mandioquinha. As amostras foram coletadas no restaurante da Universidade Estadual de Campinas, no período de abril/1998 a abril/ 2001 e nas Centrais de Abastecimento de Campinas, no período de abril a agosto/2001. Os resíduos dos agrotóxicos organoclorados foram determinados por cromatografia a gás com detector de captura de elétrons (CG-DCE- 63Ni). Os limites de detecção (LD) encontrados para Clorotalonil, Aldrin, Dieldrin, Heptacloro, Heptacloro epóxido, Endosulfan alfa, Endosulfan beta e Sulfato de Endosulfan foram 0,0006, 0,0006, 0,0008, 0,0006, 0,0007, 0,0008, 0,0009 e 0,0016 mg/kg, e os limites de quantificação 0,002, 0,002, 0,003, 0,002, 0,003, 0,003, 0,003 e 0,005 mg/kg, respectivamente. A recuperação média dos agrotóxicos analisados variou de 76,0 a 97,0% em repolho, de 90,0 a 103,0% em tomate e de 81,0 a 106,0% em batata. Em todas as amostras de hortaliças analisadas (n=151), os níveis residuais de agrotóxicos apresentaram-se abaixo dos limites de detecção (LD) do método validado. DETERMINATION OF ORGANOCHLORINE PESTICIDES RESIDUES ON VEGETABLES Abstract Residues of Chlorotalonil, Aldrin, Dieldrin, Heptachlor, Heptachlor epoxide, alpha and beta Endosulfan and Endosulfan sulfate were analysed in the following vegetables: lettuce, swiss chad, chicory, cabbage, tomato, chayote, potato, carrot, cassava and cassava specy. The samples were collected at the restaurant of the University of Campinas, from April/1998 to April/2001 at the “Centrais de Abastecimento de Campinas (CEASA)”, from April to August/2001. The organochlorine pesticide residues were determined by gas chromatography with an electron capture detector (ECD-63Ni). The detection limits of Chlorotalonil, Aldrin, Dieldrin, Heptachlor, Heptachlor epoxide, alpha and beta Endosulfan and Endosulfan sulfate were 0.0006, 0.0006, 0.0008, 0.0006, 0.0007, 0.0008, 0.0009 and 0.0016 mg/kg respectively, and their quantification limits were 0.002, 0.002, 0.003, 0.002, 0.003, 0.003, 0.003 and 0.005 mg/kg. The average recovery of the pesticides varied between 76.0 and 97.0% for cabbage, 90,0 to 103.0% for tomato and 81.0 to 106.0% for potato. The samples analysed (n=151) showed pesticide levels below the detection limits of the validated method.

scholarly journals Determination of Organophosphorus Pesticide Residues in Winter Bamboo Shoots by Homogenate Extraction Coupled with Gas Chromatography-Flame Photometric Detector

2020 ◽  
pp. 7-12
Author(s):  
Zhu Yu-Xin ◽  
Xu Liu-Yue ◽  
Feng Qi ◽  
Zhu Miao-Qin
Keyword(s):  
Gas Chromatography ◽  
Pesticide Residues ◽  
Limit Of Detection ◽  
Organophosphorus Pesticides ◽  
Flame Photometric Detector ◽  
Photometric Detector ◽  
Bamboo Shoots ◽  
Homogenate Extraction ◽  
Relative Standard

Excessive use of organophosphorus pesticides (OPPs) in fruits and vegetables may affect human health. In this paper, a simple, rapid and effective method for the determination of five OPPs in winter bamboo shoots by gas chromatography-flame photometric detector (GC-FPD) was developed. Three extractants and three extraction methods were examined respectively. The results showed that the recovery rate was higher when the samples were extracted by acetonitrile and treated with homogenate extraction method. Under the optimized conditions, recoveries ranged from 82.12% to 91.48% with the relative standard deviation (RSD) of 1.51-4.20% and the limit of detection (LOD) of 0.005-0.02 μg/mL. Results showed that using acetonitrile as extractants and homogenate extraction in sample preparation is an effective method in determination of pesticide residues in winter bamboo shoots.

scholarly journals β-Cyclodextrin Assisted Liquid–Liquid Microextraction Based on Solidification of the Floating Organic Droplets Method for Determination of Neonicotinoid Residues

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3954
Author(s):  
Vichapong ◽  
Moyakao ◽  
Kachangoon ◽  
Burakham ◽  
Santaladchaiyakit ◽  
...  
Keyword(s):  
Pesticide Residues ◽  
Limit Of Detection ◽  
Environment Friendly ◽  
Extraction Solvent ◽  
Salt Addition ◽  
High Enrichment ◽  
Sensitive Determination ◽  
Cloudy Solution ◽  
Liquid Microextraction

An efficient and environment-friendly microextraction method, namely, β-cyclodextrin assisted liquid–liquid microextraction, based on solidification of the floating organic droplets method coupled with HPLC is investigated for the sensitive determination of trace neonicotinoid pesticide residues. In this method, β-cyclodextrin is used as a disperser solvent, while 1-octanol is selected as an extraction solvent. β-cyclodextrins was found to decrease interfacial tension and increase the contact area between the organic and water phases with the help of centrifugation. A cloudy solution was rapidly formed and then centrifuged to complete phase separation. Various key parameters influencing extraction efficiency were systematically investigated and optimized; they include salt addition, concentration of β-cyclodextrin, and volume of extraction solvent (1-octanol). Under optimum conditions, good linearity was obtained with coefficient for determination (R2) greater than 0.99. A low limit of detection, high enrichment factor, and good recovery (83 – 132) were achieved. This proves that the proposed method can be applied to determine trace neonicotinoid pesticide residues in natural surface water samples.

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