ANTIBACTERIAL ACTIVITY OF PIPER CUBEBA LINN. FRUIT EXTRACTS AGAINST SELECTED BACTERIAL PATHOGENS IN BASRAH CITY

A total of 72 morphologically different actinomycetes isolates were isolated from samples collected at different regions of Vellore, Tamil Nadu, India and screened for its antibacterial activity against fish and shellfish pathogens. All actinomycetes isolates were screened for antibacterial activity by cross streak method against the selected fish and shellfish bacterial pathogens including Aeromonas caviae, Aeromonas hydroplila, Edwardsiella tarda, Vibrio anguillarum and Vibrio harveyi. Secondary screening of antagonistic isolates by well diffusion method leads to the identification of potential isolate. Culture conditions for the potential isolate were optimized for maximal growth and yield of the ethyl acetate (EA) crude extract. The potential isolate was characterized by molecular taxonomy and phylogeny and identified as Streptomyces species and named as Streptomyces sp. VITNK9. The 16S rDNA nucleotide sequence was searched through the GenBank database and showed 83% similarity to Streptomyces vinaceusdrappus. The EA extract prepared from Streptomyces sp. VITNK9 showed moderate antagonistic activity accessed by the formation of zone of growth inhibition against, Aeromonas caviae (15.33 mm), Aeromonas hydrophila (17.66 mm), Edwardsiella tarda (18.33 mm), Vibrio anguillarum (14.33 mm) and Vibrio harveyi (14.33 mm). The MIC value of EA extract was ranged between 0.03-0.125 mg mL-1. The GC-MS spectrum of the ethyl acetate extract revealed the presence of two major compounds, pyrrolo [1,2-A] pyrazine-1,4-Dione (56.67%) and Hexahydro-3-(2-Methylpropyl) (27.91%), respectively. The results of the study suggest that Streptomyces sp. VITNK9 is a potential source for antagonistic secondary metabolites against fish and shellfish bacterial pathogens.

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ANTIMICROBIAL, ANTIRADICAL AND INSECTICIDAL ACTIVITY OF GARDENIA GUMMIFERA L. F. (RUBIACEAE)

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i10.20252 ◽

2017 ◽

Vol 9 (10) ◽

pp. 265 ◽

Cited By ~ 1

Author(s):

Prashith Kekuda T. R. ◽

Raghavendra H. L. ◽

Shilpa M. ◽

Pushpavathi D. ◽

Tejaswini Petkar ◽

...

Keyword(s):

Antibacterial Activity ◽

Insecticidal Activity ◽

Leaf Extract ◽

Antiradical Activity ◽

Radical Scavenging ◽

Fruit Extract ◽

Fruit Extracts ◽

Ic50 Value ◽

Agar Well Diffusion Assay ◽

Dose Dependent

Objective: The present study was carried out to investigate antimicrobial, antiradical and insecticidal potential of leaf and fruit of Gardenia gummifera L. f. (Rubiaceae).Methods: The leaf and fruits were shade dried, powdered and extracted by maceration process using methanol. Antibacterial activity was evaluated against Gram positive and Gram negative bacteria by Agar well diffusion assay. Antifungal activity was determined against six seed-borne fungi by Poisoned food technique. Antiradical activity of leaf and fruit extracts was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS) radical scavenging assays. Insecticidal activity of leaf and fruit extracts, in terms of larvicidal and pupicidal activity, was assessed against larvae and pupae of Aedes aegypti.Results: Both the extracts inhibited all test bacteria. Marked antibacterial activity was displayed by fruit extract when compared to leaf extract. S. epidermidis and E. coli were inhibited to highest and least extent by both extracts respectively. Fruit extract was found to exhibit higher antifungal effect when compared to leaf extract. Leaf extract and fruit extract exhibited highest inhibitory activity against A. niger and A. flavus respectively. Leaf and fruit extracts scavenged DPPH radical’s dose dependently with an IC50 value of 49.01µg/ml and 2.53µg/ml respectively. The scavenging of ABTS by leaf and fruit extracts was dose dependent and the IC50 value for leaf and fruit extract was 2.58µg/ml and 2.31µg/ml respectively. Fruit extract was shown to exhibit marked antiradical activity when compared to leaf extract. Leaf and fruit extracts exhibited dose dependent insecticidal activity in terms of larvicidal and pupicidal activity and the susceptibility of larvae and pupae to extracts was in the order II instar larvae>IV instar larvae>pupae. Fruit extract displayed marked insecticidal potential when compared to leaf extract.Conclusion: Overall, fruit extract of G. gummifera exhibited marked antimicrobial, antiradical and insecticidal activity when compared to leaf extract. The plant can be used for developing agents/formulations effective against infectious microorganisms, oxidative stress and insect vectors that transmit dreadful diseases. The observed bioactivities could be ascribed to the presence of active principles which are to be isolated and characterized.

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STUDY ON THE ANTIBACTERIAL ACTIVITY OF FRUIT EXTRACTS OF KLUTUK BANANA (MUSA BALBISIANA COLLA) AGAINST SHIGELLA DYSENTERIAE ATCC 13313

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i7.18561 ◽

2017 ◽

Vol 10 (7) ◽

pp. 220 ◽

Cited By ~ 5

Author(s):

Sriagung Fitri Kusuma ◽

Soraya R Mita ◽

Indah Firdayani ◽

Resmi Mustarichie

Keyword(s):

Antibacterial Activity ◽

Thin Layer ◽

Ethanol Extract ◽

Shigella Dysenteriae ◽

Thin Layer Plate ◽

Phytochemical Analysis ◽

Banana Fruit ◽

Musa Balbisiana ◽

Fruit Extracts ◽

Microdilution Method

Objective: The purpose of this study is to determine the antibacterial activity of Klutuk Banana (Musa balbisiana colla) fruit extracts against Shigella dysenteriae ATCC 13313 and the amount of potassium to the discovery of anti-dysentery drug candidates.Methods: The simplisia of Klutuk banana fruit was extracted with ethanol using a maceration method. The phytochemical screening of ethanol extract was performed using standard procedures. Determination thin layer chromatography (TLC) profile of the extract was performed using a thin layer plate. The antibacterial activity was investigated using agar well diffusion technique. The minimum inhibitory concentrations (MIC) were determined by a serial microdilution method, whereas the minimum bactericidal concentration (MBC) was done by subculturing the MIC result onto agar medium. Potassium levels of the extract were carried out quantitatively using atomic absorption spectrophotometry.Results: The phytochemical analysis revealed the presence of flavonoids, polyphenols, tannins, monoterpenoid and sesquiterpenoids, quinones, and saponins. The TLC results prove the existence of flavonoids in the tested extract. The content of secondary metabolites that can act as an antibacterial, strengthen the antibacterial activity of ethanol extract of Klutuk banana against S. dysenteriae 13313 with MBC values in the range of 5-10%w/v. Potassium levels in the ethanol extract of Klutuk banana fruits contain potassium as much as 2.919% (29 190 ppm).Conclusion: It can be concluded that the ethanol extract of Klutuk banana fruits is more potent as antibacterial against S. dysenteriae than as potassium supplier in hypokalemia therapy.

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