In vitro assessment of antioxidative potential of goat milk, casein and its hydrolysates: Comparison of goat milk with bovine and buffalo milk

Abstract The present study was executed with an aim to explore the antioxidative potential of goat, cow, and buffalo milk. Buffalo milk has showed highest antioxidative potential than goat and cow milk as measured by ABTS, ORAC, and DPPH assays, whereas goat milk has showed better antioxidative potential than cow milk when measured by ORAC and DPPH. Further, the effect of temperature on the antioxidative potential of goat milk was assessed. An increase in temperature has a negatively affect the antioxidative potential of goat milk. The antioxidative potential of goat milk was in the following order: raw milk > pasteurized milk > boiled milk. Casein derived from goat milk by isoelectric precipitation was hydrolyzed by gastrointestinal enzymes pepsin (P), trypsin (T), chymotrypsin (C), and their combinations PT, PC, TC, and PTC. Among all the casein hydrolysates, the maximum antioxidative potential was found in PT hydrolysate, further fractionated by 10, 3 and 1 kDa ultrafiltration membranes. 3–10 kDa fraction exhibited maximum antioxidative potential in comparison to other fractions of PT hydrolysate. Our results suggested that antioxidative potential of goat milk and its hydrolysates could be an important mean to obtain natural antioxidative peptides.

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An in-vitro assessment of antifungal and antibacterial activity of cow, camel, ewe, and goat milk kefir and probiotic yogurt

Journal of Food Measurement & Characterization ◽

10.1007/s11694-020-00645-4 ◽

2020 ◽

Cited By ~ 1

Author(s):

Maryam Azizkhani ◽

Per Erik Joakim Saris ◽

Mehdi Baniasadi

Keyword(s):

Antibacterial Activity ◽

Goat Milk ◽

Probiotic Yogurt ◽

In Vitro Assessment

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SCREENING, CHARACTERIZATION, AND IN VITRO EVALUATION OF PROBIOTIC PROPERTIES OF LACTOBACILLUS STRAINS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i8.14233 ◽

2017 ◽

Vol 10 (8) ◽

pp. 288 ◽

Cited By ~ 1

Author(s):

Neha Jain ◽

Archana Mehata ◽

Vandana Bharti

Keyword(s):

Antimicrobial Activity ◽

Bile Salt ◽

Goat Milk ◽

Buffalo Milk ◽

Cattle Dung ◽

Probiotic Properties ◽

Gelatinase Activity ◽

Lactobacillus Pentosus ◽

Gram Staining

Objective: The aim of the present investigation was to isolate and identify Lactobacillus strains from dairy and cattle dung samples. Potent isolates were selected for screening by antimicrobial activity; selected lactobacilli were further tested for probiotic properties and adhesive attributes.Methods: Lactobacilli were isolated aseptically on specific de man, rogosa and sharpe medium from dairy and cattle dung samples. Isolates were identified by Gram-staining, motility, catalase, endospore, and carbohydrate fermentation tests. Further, the isolates were screened for antimicrobial activity by disk diffusion assay, and potent lactobacilli were observed for probiotic properties: Acid and bile salt tolerance, gelatinase activity, and autolytic activity. For analyzing the adhesive attributes, isolates were observed for autoaggregation, coaggregation and microbial adhesion to solvents assay.Results: About 12 Lactobacillus strains among 98 isolates exhibited maximum antimicrobial activity were further selected for identifying their probiotic and adhesive attributes. Among 12 selected isolates, cell-free supernatant (CFS) of buffalo milk BM10 and goat milk GM10 showed excellent antimicrobial activity, 20.34±0.02 mm against Staphylococcus aureus and 18.65±0.11 mm against Escherichia coli. Isolates showed survival at pH 2 and 3 and can tolerate 0.2-0.3% bile salt concentrations. The GM5 showed maximum autoaggregation (67.04±0.61%) and minimum coaggregation (11.51±0.50%) showed by GM3. The BM10 exhibited maximum adherent value 64.84±1.41% for n-hexadecane.Conclusion: The two lactobacilli, BM10 and GM10 identified as Lactobacillus fermentum and Lactobacillus pentosus on the basis of phenotypic and sugar utilization tests. The CFS of both lactobacilli can be used as antimicrobial agent. Both isolates showed significant results of probiotic and adhesive attributes, therefore, can be evaluated for clinical and therapeutic applications.

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