scholarly journals Global Proteomics Revealed Klebsiella pneumoniae Induced Autophagy and Oxidative Stress in Caenorhabditis elegans by Inhibiting PI3K/AKT/mTOR Pathway during Infection

2017 ◽  
Vol 7 ◽  
Author(s):  
Arumugam Kamaladevi ◽  
Krishnaswamy Balamurugan
Keyword(s):  
Oxidative Stress ◽  
Caenorhabditis Elegans ◽  
Klebsiella Pneumoniae ◽  
Mtor Pathway ◽  
And Oxidative Stress

scholarly journals Effects of electrotactic exercise and antioxidant EUK-134 on oxidative stress relief in Caenorhabditis elegans

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245474
Author(s):  
Thi Thanh Huong Pham ◽  
Wan-Ying Huang ◽  
Chang-Shi Chen ◽  
Wen-Tai Chiu ◽  
Han-Sheng Chuang
Keyword(s):  
Oxidative Stress ◽  
Caenorhabditis Elegans ◽  
Intracellular Signaling ◽  
Stress Effect ◽  
Wild Type ◽  
Antioxidant Supplementation ◽  
Antioxidative Stress ◽  
Biochemical Indices ◽  
And Oxidative Stress

Antioxidant uptake and regular exercise are two well-acknowledged measures used for rejuvenation and oxidative stress elimination. Previous studies have revealed that moderate exercise mildly increases intracellular signaling oxidant levels and strengthens the ability of an organism to deal with escalating oxidative stress by upregulating antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase. Antioxidant supplementation directly scavenges intracellular reactive oxygen species (ROS) to reduce oxidative stress. However, research to understand the impacts of these enzymes on mitigating oxidative stress from the perspective of simple animals is limited. Herein, we show that exercise combined with antioxidant supplementation ameliorates the physiological phenotypes and markers of aging in wild-type and SOD/CAT-deficient Caenorhabditis elegans. We discovered that treated wild-type and gene-deficient worms show better survivorship, reproduction, and motility compared with their control counterparts. Assays of biochemical indices revealed that variations in sod-3 expression under different stress levels imply an inducible enzyme response resulting from exercise training and antioxidant supplementation. In addition, induced ROS resistance obtained from any type of treatment could persist for several days even after treatment cessation, thus suggesting a potential long-term antioxidative stress effect. Our findings confirm that exercise, antioxidant supplementation, and their combination could significantly improve the ability of C. elegans to withstand adverse stress. Our observations provide promising insights into future therapies of anti-oxidative stress in higher animals.

scholarly journals Correction: A Novel Role for the SMG-1 Kinase in Lifespan and Oxidative Stress Resistance in Caenorhabditis elegans

PLoS ONE ◽  
2009 ◽  
Vol 4 (1) ◽  
Author(s):  
Ingrid Masse ◽  
Laurent Molin ◽  
Laurent Mouchiroud ◽  
Philippe Vanhems ◽  
Francesca Palladino ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Caenorhabditis Elegans ◽  
Stress Resistance ◽  
Oxidative Stress Resistance ◽  
And Oxidative Stress

scholarly journals Autophagy R Educes B Acterial T Ranslocation by Attenuating Oxidative Stress in Hypervirulent Klebsiella Pneumoniae-Caused Liver Abscesses Mice

2021 ◽  
Author(s):  
Xing Lu ◽  
Xianlong Wang ◽  
Jie Xu ◽  
Chengfen Yin ◽  
Peng Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Klebsiella Pneumoniae ◽  
Intestinal Mucosa ◽  
Intestinal Flora ◽  
Mucosal Barrier ◽  
Ultrastructural Changes ◽  
Sequencing Analysis ◽  
Liver Abscesses ◽  
Rdna Sequencing ◽  
And Oxidative Stress

Abstract Background Translocation of intestinal flora can cause liver abscesses.The epidemiological data were mainly Kebsiella pneumoniae infection.It is usually associated with changes in mucosal autophagy and oxidative stress.Objective The aim of this study was to investigate the correlation between autophagy and oxidative stress on the intestinal mucosal barrier of hypervirulent Klebsiella pneumoniae-caused liver abscesses(hvKp-cla) mice model. And the genes that might be involved.Methods C57BL/6J mice were used as study subjects to induce liver abscesses model by hypervirulent Klebsiella pneumoniae gavage. Bacterial translocation (BT) was detected by 16S rDNA sequencing analysis.Morphological alterations in the liver and gut were assessed by hematoxylin–eosin staining.Oxidative stress status was determined by measuring the level of intestinal malondialdehyde (MDA),superoxide dismutase (SOD) and glutathione peroxidase (GPx).In situ hybridization was used to determine whether the bacteria had migrated to the liver.Western blot, RT-PCR,and immunofuorescent staining were preformed to analyze the expression of tight junction and autophagy proteins. The ultrastructural changes of liver were examined by electron microscopy.RNA-seq was used to detect the possible involved genes. Results According to the sequencing analysis, mice were divided into BT (+) group (n = 7) and BT (-) group(n = 7).The damage of intestinal mucosa and liver in BT(+) group was more serious than that in BT(-) group. The translocated Klebsiella pneumoniae was observed in the intestinal mucosa lamina propria and liver.The content of MDA was clearly elevated, and SOD as well as GPx activities were decreased in BT (+) group as compared with BT (-) group.The expression of LC3II and Beclin1 in BT (-) group was higher than that observed in BT (+). In contrast, BT (+)group had a lower level of Zonulin-1 (ZO-1) and claudin-2. RNA-seq found 1912 genes were up-regulated and 1,911 genes down-regulated. Those genes of mTOR,Atg4b and SERPINA3 were involved. Conclusion Autophagy reduces intestinal hypervirulent Klebsiella pneumoniae translocation by reducing oxidative stress levels. Those genes of mTOR,Atg4b and SERPINA3 were involved.

Caenorhabditis elegans: A promising contrivance to study neurotoxicity and oxidative stress

2021 ◽  
Vol 16 (10) ◽  
pp. 198-206
Author(s):  
Kiran Singh ◽  
Shweta Yadav
Keyword(s):  
Oxidative Stress ◽  
Caenorhabditis Elegans ◽  
Genetic Manipulation ◽  
Model Organism ◽  
Mechanisms Of Toxicity ◽  
C Elegans ◽  
Toxicological Studies ◽  
And Oxidative Stress

Owing to ubiquitous distribution, high abundances and ecological relevance, Caenorhabditis elegans has strong potential interest as barometer of environment and human health. Ecotoxicological methods are used to evaluate the effect of various anthropogenic contaminants on the ecosystems that circumscribe both in-vivo and in-vitro toxicities to explore the pathways and mechanisms of toxicity and to set precise toxicity thresholds. The interest in C. elegans, as a model organism in toxicological studies, has increased over the past few decades. The enticement of C. elegans comes from the ease of metabolically active digestive, sensory, endocrine, neuromuscular, reproductive systems and genetic manipulation along with the ability to fluorescently label neuronal subtypes. The study reviews the competence of Caenorhabditis elegans as a potential model organism in various toxicity assays specifically neurotoxicity and oxidative stress.

scholarly journals Effects of sub-inhibitory concentrations of antibiotics and oxidative stress on the expression of type II toxin-antitoxin system genes in Klebsiella pneumoniae

2020 ◽  
Vol 21 ◽  
pp. 51-56 ◽  
Author(s):  
Negar Narimisa ◽  
Fatemeh Amraei ◽  
Behrooz Sadeghi Kalani ◽  
Rokhsareh Mohammadzadeh ◽  
Faramarz Masjedian Jazi
Keyword(s):  
Oxidative Stress ◽  
Klebsiella Pneumoniae ◽  
Type Ii ◽  
And Oxidative Stress

scholarly journals Tart Cherry Increases Lifespan in Caenorhabditis elegans by Altering Metabolic Signaling Pathways

Nutrients ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1482
Author(s):  
Shasika Jayarathne ◽  
Latha Ramalingam ◽  
Hunter Edwards ◽  
Siva A. Vanapalli ◽  
Naima Moustaid-Moussa
Keyword(s):  
Oxidative Stress ◽  
Caenorhabditis Elegans ◽  
Mitochondrial Function ◽  
Antioxidant Properties ◽  
Wild Type ◽  
Tart Cherry ◽  
C Elegans ◽  
Beneficial Effects ◽  
The Mean ◽  
And Oxidative Stress

Aging and healthspan are determined by both environmental and genetic factors. The insulin/insulin-like growth factor-1(IGF-1) pathway is a key mediator of aging in Caenorhabditis elegans and mammals. Specifically, DAF-2 signaling, an ortholog of human IGF, controls DAF-16/FOXO transcription factor, a master regulator of metabolism and longevity. Moreover, mitochondrial dysfunction and oxidative stress are both linked to aging. We propose that daily supplementation of tart cherry extract (TCE), rich in anthocyanins with antioxidant properties may exert dual benefits for mitochondrial function and oxidative stress, resulting in beneficial effects on aging in C. elegans. We found that TCE supplementation at 6 μg or 12 μg/mL, increased (p < 0.05) the mean lifespan of wild type N2 worms, respectively, when compared to untreated control worms. Consistent with these findings, TCE upregulated (p < 0.05) expression of longevity-related genes such as daf-16 and aak-2 (but not daf-2 or akt-1 genes) and genes related to oxidative stress such as sod-2. Further, we showed that TCE supplementation increased spare respiration in N2 worms. However, TCE did not change the mean lifespan of daf-16 and aak-2 mutant worms. In conclusion, our findings indicate that TCE confers healthspan benefits in C. elegans through enhanced mitochondrial function and reduced oxidative stress, mainly via the DAF-16 pathway.

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