scholarly journals Effects of sub-inhibitory concentrations of antibiotics and oxidative stress on the expression of type II toxin-antitoxin system genes in Klebsiella pneumoniae

2020 ◽  
Vol 21 ◽  
pp. 51-56 ◽  
Author(s):  
Negar Narimisa ◽  
Fatemeh Amraei ◽  
Behrooz Sadeghi Kalani ◽  
Rokhsareh Mohammadzadeh ◽  
Faramarz Masjedian Jazi
Keyword(s):  
Oxidative Stress ◽  
Klebsiella Pneumoniae ◽  
Type Ii ◽  
And Oxidative Stress

scholarly journals Undenatured Type II Collagen Relieves Bone Impairment through Improving Inflammation and Oxidative Stress in Ageing db/db Mice

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4942
Author(s):  
Rui Fan ◽  
Yuntao Hao ◽  
Xinran Liu ◽  
Jiawei Kang ◽  
Jiani Hu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Cytokines ◽  
Intervention Group ◽  
Type Ii Collagen ◽  
Tartrate Resistant Acid Phosphatase ◽  
Type Ii ◽  
Mineral Density ◽  
Ageing Model ◽  
And Oxidative Stress ◽  
Undenatured Type Ii Collagen

Ageing-related bone impairment due to exposure to hyperglycemic environment is scarcely researched. The aim was to confirm the improvement effects of undenatured type II collagen (UC II) on bone impairment in ageing db/db mice, and the ageing model was established by normal feeding for 48-week-old. Then, the ageing db/db mice were randomly assigned to UC II intervention, the ageing model, and the chondroitin sulfate + glucosamine hydrochloride control groups. After 12 weeks of treatment, femoral microarchitecture and biomechanical parameters were observed, biomarkers including bone metabolism, inflammatory cytokines, and oxidative stress were measured, and the gastrocnemius function and expressions of interleukin (IL) 1β, receptor activator of nuclear factor (NF)-κB ligand (RANKL), and tartrate-resistant acid phosphatase (TRAP) were analyzed. The results showed that the mice in the UC II intervention group showed significantly superior bone and gastrocnemius properties than those in the ageing model group, including bone mineral density (287.65 ± 72.77 vs. 186.97 ± 32.2 mg/cm3), gastrocnemius index (0.46 ± 0.07 vs. 0.18 ± 0.01%), muscle fiber diameter (0.0415 ± 0.005 vs. 0.0330 ± 0.002 mm), and cross-sectional area (0.0011 ± 0.00007 vs. 0.00038 ± 0.00004 mm2). The UC II intervention elevated bone mineralization and formation and decreased bone resorption, inflammatory cytokines, and the oxidative stress. In addition, lower protein expression of IL-1β, RANKL, and TRAP in the UC II intervention group was observed. These findings suggested that UC II improved bones impaired by T2DM during ageing, and the likely mechanism was partly due to inhibition of inflammation and oxidative stress.

scholarly journals Autophagy R Educes B Acterial T Ranslocation by Attenuating Oxidative Stress in Hypervirulent Klebsiella Pneumoniae-Caused Liver Abscesses Mice

2021 ◽  
Author(s):  
Xing Lu ◽  
Xianlong Wang ◽  
Jie Xu ◽  
Chengfen Yin ◽  
Peng Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Klebsiella Pneumoniae ◽  
Intestinal Mucosa ◽  
Intestinal Flora ◽  
Mucosal Barrier ◽  
Ultrastructural Changes ◽  
Sequencing Analysis ◽  
Liver Abscesses ◽  
Rdna Sequencing ◽  
And Oxidative Stress

Abstract Background Translocation of intestinal flora can cause liver abscesses.The epidemiological data were mainly Kebsiella pneumoniae infection.It is usually associated with changes in mucosal autophagy and oxidative stress.Objective The aim of this study was to investigate the correlation between autophagy and oxidative stress on the intestinal mucosal barrier of hypervirulent Klebsiella pneumoniae-caused liver abscesses(hvKp-cla) mice model. And the genes that might be involved.Methods C57BL/6J mice were used as study subjects to induce liver abscesses model by hypervirulent Klebsiella pneumoniae gavage. Bacterial translocation (BT) was detected by 16S rDNA sequencing analysis.Morphological alterations in the liver and gut were assessed by hematoxylin–eosin staining.Oxidative stress status was determined by measuring the level of intestinal malondialdehyde (MDA),superoxide dismutase (SOD) and glutathione peroxidase (GPx).In situ hybridization was used to determine whether the bacteria had migrated to the liver.Western blot, RT-PCR,and immunofuorescent staining were preformed to analyze the expression of tight junction and autophagy proteins. The ultrastructural changes of liver were examined by electron microscopy.RNA-seq was used to detect the possible involved genes. Results According to the sequencing analysis, mice were divided into BT (+) group (n = 7) and BT (-) group(n = 7).The damage of intestinal mucosa and liver in BT(+) group was more serious than that in BT(-) group. The translocated Klebsiella pneumoniae was observed in the intestinal mucosa lamina propria and liver.The content of MDA was clearly elevated, and SOD as well as GPx activities were decreased in BT (+) group as compared with BT (-) group.The expression of LC3II and Beclin1 in BT (-) group was higher than that observed in BT (+). In contrast, BT (+)group had a lower level of Zonulin-1 (ZO-1) and claudin-2. RNA-seq found 1912 genes were up-regulated and 1,911 genes down-regulated. Those genes of mTOR,Atg4b and SERPINA3 were involved. Conclusion Autophagy reduces intestinal hypervirulent Klebsiella pneumoniae translocation by reducing oxidative stress levels. Those genes of mTOR,Atg4b and SERPINA3 were involved.

scholarly journals Correlation of Leptin, Proinflammatory Cytokines and Oxidative Stress with Tumor Size and Disease Stage of Endometrioid (Type I) Endometrial Cancer and Review of the Underlying Mechanisms

Cancers ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 268
Author(s):  
Clelia Madeddu ◽  
Elisabetta Sanna ◽  
Giulia Gramignano ◽  
Luciana Tanca ◽  
Maria Cristina Cherchi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endometrial Cancer ◽  
Prognostic Factors ◽  
Cancer Patients ◽  
Proinflammatory Cytokines ◽  
Disease Stage ◽  
Type I ◽  
Type Ii ◽  
Endometrioid Endometrial Cancer ◽  
And Oxidative Stress

Endometrioid endometrial cancer is associated with increased BMI and obesity through multiple pathogenetic mechanisms involving hyperestrogenism, hyperinsulinemia, altered adipokine secretion, inflammation, and oxidative stress. In the present study, we aimed to investigate the correlation between BMI, leptin, the proinflammatory cytokines IL-6 and TNFα, reactive oxygen species (ROS), and the traditional prognostic factors T, G, N and M status among type I endometrioid and type II endometrial cancer patients. We enrolled 305 consecutive endometrial cancer patients prospectively. We found that BMI, leptin, and IL-6 significantly correlated with T status, N status, and M status among endometrioid type I endometrial cancer patients. Among type II endometrial cancer patients, BMI and leptin did not correlate with any of the prognostic parameters, whereas there was a positive correlation between IL-6 and the presence of distant metastases. In the multivariate regression analysis, BMI, leptin, and IL-6 were independent predictive variables of T, N, and M status in endometrioid type I endometrial cancer patients. Our study demonstrates that weight gain, adiposity-related adipokines, inflammation, and oxidative stress correlate with the prognostic factors of endometrioid endometrial cancer. Knowledge of the role of obesity-related biological pathways and mediators in the pathogenesis and prognosis of endometrioid endometrial malignancies may offer new perspectives on combined therapeutic strategies that have not been explored to date, both in the advanced disease and in the adjuvant setting.

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