The EuroFlow PID Orientation Tube for Flow Cytometric Diagnostic Screening of Primary Immunodeficiencies of the Lymphoid System

Osmotic Fragility Test (OFT) is widely considered as a sensitive indicator of red blood cells sensitivity to the hypotonic solution. Traditional osmotic fragility test (Dacie and Lewis) is time and work-consuming, and need relatively large minimum volume of peripheral blood for proper test performance. It does not belong to the most popular tests in a daily laboratory practice. The purpose of this article is to underline the diagnostic value of the Osmotic Fragility Test as well as present the latest methods that improves the traditional technique, such as Acidified Glycerol Lysis Test (AGLT 50), Pink Test, or Flow Cytometric Osmotic Fragility Test (FCM OF Test). Perhaps a new, fresh view at the issue in the nearest future will contribute to reconsider the osmotic fragility test for routine diagnostic screening of red blood cell disorders in children and adults.

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Flow Cytometric Approach in the Diagnosis of Primary Immunodeficiencies

Cell Interaction - Molecular and Immunological Basis for Disease Management ◽

10.5772/intechopen.96004 ◽

2021 ◽

Author(s):

Sevil Oskay Halacli

Keyword(s):

Flow Cytometry ◽

Protein Expression ◽

Adaptive Immune System ◽

Primary Immunodeficiencies ◽

Cellular Functions ◽

Adaptive Immune ◽

Flow Cytometric ◽

Protein Expressions ◽

And Function

Primary Immunodeficiencies (PIDs) compose of a large spectrum of diseases characterized by abrogated or dysregulated functions of innate and adaptive immune system components that cause susceptibility to recurrent infections, autoimmunity, neoplasia/malignancy and dysfunction of organs and skeletal system. PIDs are also evaluated as molecular diseases due to the mutations in one or more genes. That affects transcripts and protein expressions as well as their functions. Today, 430 different genes are known to have various functional effects which are related to 403 different PIDs. Analyzing the effects of the mutations on relevant protein expression and function is significant to diagnose and the follow-up of the PIDs. Application of flow cytometry for analyzing protein expression levels and functions in immune cells as well as investigating the cellular functions tender a rapid, quantitative and reliable approach to identify and to prove the genetic background of PIDs. Therefore, the use of flow cytometry aids to have a large spectrum of data from gene to function and from function to clinical relevance in the first-step and differantial diagnosis of PIDs.

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IVBT-documented platelet function correlates with flow cytometric data

Transfusion Science ◽

10.1016/s0955-3886(96)90091-0 ◽

1996 ◽

Vol 17 (4) ◽

pp. 553-558 ◽

Cited By ~ 2

Author(s):

J HOFFMANN

Keyword(s):

Platelet Function ◽

Flow Cytometric Data ◽

Flow Cytometric

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Re: Lymph node sampling for flow cytometric analysis

Pathology ◽

10.1080/00313020307577 ◽

2003 ◽

Vol 35 (2) ◽

pp. 183-183

Author(s):

Ibrahim Zardawi

Keyword(s):

Lymph Node ◽

Flow Cytometric Analysis ◽

Lymph Node Sampling ◽

Flow Cytometric

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Review of Diagnostic Screening Instruments for Alcohol and Other Drug Use and Other Psychiatric Disorders

PsycEXTRA Dataset ◽

10.1037/e674282010-001 ◽

2002 ◽

Cited By ~ 25

Author(s):

Sharon Dawe ◽

Natalie J. Loxton ◽

Leanne Hides ◽

David J. Kavanagh ◽

Richard P. Mattick

Keyword(s):

Drug Use ◽

Psychiatric Disorders ◽

Screening Instruments ◽

Diagnostic Screening

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Comparative analysis of flow cytometric methods for quantitating and phenotyping apoptotic peripheral lymphocytes from HIV-infected persons

Immunology Letters ◽

10.1016/s0165-2478(97)87372-5 ◽

1997 ◽

Vol 56 (1-3) ◽

pp. 134

Author(s):

H Lecoeur

Keyword(s):

Comparative Analysis ◽

Peripheral Lymphocytes ◽

Flow Cytometric

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Flow cytometric enumeration and immunophenotyping of hematopoietic stem and progenitor cells

Seminars in Hematology ◽

10.1053/shem.2001.21925 ◽

2001 ◽

Vol 38 (2) ◽

pp. 139-147

Author(s):

Jan W. Gratama ◽

D. Robert Sutherland ◽

Michael Keeney

Keyword(s):

Progenitor Cells ◽

Hematopoietic Stem ◽

Flow Cytometric ◽

Stem And Progenitor Cells

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Expression of Platelet Membrane Glycoprotein V in Human Megakaryocytes and Megakaryocytic Cell Lines: A Study Using a Novel Monoclonal Antibody against GPV

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648955 ◽

1994 ◽

Vol 72 (05) ◽

pp. 762-769 ◽

Cited By ~ 11

Author(s):

Toshiro Takafuta ◽

Kingo Fujirmura ◽

Hironori Kawano ◽

Masaaki Noda ◽

Tetsuro Fujimoto ◽

...

Keyword(s):

Monoclonal Antibody ◽

Cell Lines ◽

Immunohistochemical Analysis ◽

Specific Protein ◽

Platelet Membrane ◽

Rt Pcr ◽

Chain Reaction ◽

Flow Cytometric ◽

Polymerase Chain ◽

Megakaryocytic Cell

SummaryGlycoprotein V (GPV) is a platelet membrane protein with a molecular weight of 82 kD, and one of the leucine rich glycoproteins (LRG). By reverse transcription-polymerase chain reaction (RT-PCR), GPV cDNA was amplified from mRNA of platelets and megakaryocytic cell lines. However, since there are few reports indicating whether GPV protein is expressed in megakaryocytes as a lineage and maturation specific protein, we studied the GPV expression at the protein level by using a novel monoclonal antibody (1D9) recognizing GPV. Flow cytometric and immunohistochemical analysis indicated that GPV was detected on the surface and in the cytoplasm of only the megakaryocytes in bone marrow aspirates. In a megakaryocytic cell line UT-7, GPV antigen increased after treatment with phorbol-12-myri-state-13-acetate (PMA). These data indicate that only megakaryocytes specifically express the GPV protein among hematopoietic cells and that the expression of GPV increases with differentiation of the megakaryocyte as GPIb-IX complex.

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