scholarly journals Past and Present Approaches to Diagnosis of Active Pulmonary Tuberculosis

2021 ◽  
Vol 8 ◽  
Author(s):  
Anna Ritah Namuganga ◽  
Novel N. Chegou ◽  
Harriet Mayanja-Kizza
Keyword(s):  
Pulmonary Tuberculosis ◽  
Sensitivity And Specificity ◽  
Diagnostic Tests ◽  
Selection Criteria ◽  
Diagnostic Methods ◽  
Ongoing Research ◽  
Active Pulmonary Tuberculosis ◽  
Mortality Burden ◽  
Short Time ◽  
Tuberculosis Disease

Tuberculosis disease continues to contribute to the mortality burden globally. Due to the several shortcomings of the available diagnostic methods, tuberculosis disease continues to spread. The difficulty to obtain sputum among the very ill patients and the children also affects the quick diagnosis of tuberculosis disease. These challenges warrant investigating different sample types that can provide results in a short time. Highlighted in this review are the approved pulmonary tuberculosis diagnostic methods and ongoing research to improve its diagnosis. We used the PRISMA guidelines for systematic reviews to search for studies that met the selection criteria for this review. In this review we found out that enormous biosignature research is ongoing to identify host biomarkers that can be used as predictors of active PTB disease. On top of this, more research was also being done to improve already existing diagnostic tests. Host markers required more optimization for use in different settings given their varying sensitivity and specificity in PTB endemic and non-endemic settings.

scholarly journals Diagnosis of Feline Infectious Peritonitis: A Review of the Current Literature

Viruses ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1068 ◽  
Author(s):  
Sandra Felten ◽  
Katrin Hartmann
Keyword(s):  
Sensitivity And Specificity ◽  
Diagnostic Tests ◽  
Clinical Signs ◽  
Etiologic Agent ◽  
Diagnostic Methods ◽  
Sample Material ◽  
Feline Infectious Peritonitis ◽  
Veterinary Practitioner ◽  
Laboratory Changes ◽  
Feline Coronavirus

Feline infectious peritonitis (FIP) is a fatal disease that poses several challenges for veterinarians: clinical signs and laboratory changes are non-specific, and there are two pathotypes of the etiologic agent feline coronavirus (FCoV), sometimes referred to as feline enteric coronavirus (FECV) and feline infectious peritonitis virus (FIPV) that vary fundamentally in their virulence, but are indistinguishable by a number of diagnostic methods. This review focuses on all important steps every veterinary practitioner has to deal with and new diagnostic tests that can be considered when encountering a cat with suspected FIP with the aim to establish a definitive diagnosis. It gives an overview on all available direct and indirect diagnostic tests and their sensitivity and specificity reported in the literature in different sample material. By providing summarized data for sensitivity and specificity of each diagnostic test and each sample material, which can easily be accessed in tables, this review can help to facilitate the interpretation of different diagnostic tests and raise awareness of their advantages and limitations. Additionally, diagnostic trees depict recommended diagnostic steps that should be performed in cats suspected of having FIP based on their clinical signs or clinicopathologic abnormalities. These steps can easily be followed in clinical practice.

Implications of covariate induced test dependence on the diagnostic accuracy of latent class analysis in pulmonary tuberculosis

2021 ◽  
Author(s):  
Alfred Kipyegon Keter ◽  
Lutgarde Lynen ◽  
Alastair van Heerden ◽  
Els Goetghebeur ◽  
Bart K.M. Jacobs
Keyword(s):  
Pulmonary Tuberculosis ◽  
Diagnostic Accuracy ◽  
Diagnostic Test ◽  
Sensitivity And Specificity ◽  
Diagnostic Tests ◽  
Latent Class ◽  
Test Accuracy ◽  
Test Results ◽  
Test Sensitivity ◽  
Correct Estimation

Abstract Background Lack of a perfect reference standard for pulmonary tuberculosis (PTB) diagnosis complicates assessment of accuracy of new diagnostic tests. Alternative strategies such as discrepant resolution and use of composite reference standards may lead to incorrect inferences on disease prevalence and diagnostic test sensitivity and specificity. Latent class analysis (LCA), a statistical method for analyzing diagnostic test results in the absence of a gold standard, allows correct estimation under strict assumptions. The model assumes that the diagnostic tests are independent conditional on the true disease status and that the diagnostic test sensitivity and specificity remain constant across subpopulations. These assumptions are violated when a factor such as severe comorbidity affects the prevalence and/or alters the diagnostic test performance. We aim to provide guidance on correct estimation of the prevalence and diagnostic test accuracy based on LCA when a known factor induces dependence among the diagnostic tests. If unaccounted for, this dependence may lead to misleading inferences. Methods Through likelihood evaluation and simulation we examined implications of likely model violations on estimation of prevalence, sensitivity and specificity among passive case-finding presumptive PTB patients with or without HIV. We generated independent results for five diagnostic tests conditional on PTB and HIV. We performed Bayesian LCA, separately for five and three diagnostic tests using four working models with or without constant PTB prevalence and diagnostic test accuracy across HIV subpopulations. Results In evaluating three diagnostic tests, the models accounting for heterogeneity in diagnostic accuracy produced consistent estimates while the models ignoring it produced biased estimates. The model ignoring heterogeneity in PTB prevalence is less problematic. When evaluating five diagnostic tests, the models were robust to violation of the assumptions. Conclusions Well-chosen covariate-specific adaptations of the model can avoid bias implied by recognized heterogeneity in PTB patient populations generating otherwise dependent test results in LCA.

Diagnosis of Caprine Arthritis Encephalitis Virus infection in dairy goats by ELISA, PCR and Viral Culture

2017 ◽  
Vol 20 (2) ◽  
pp. 347-353 ◽  
Author(s):  
S. Panneum ◽  
T. Rukkwamsuk
Keyword(s):  
Sensitivity And Specificity ◽  
Test Performance ◽  
Diagnostic Tests ◽  
Antigenic Variation ◽  
Encephalitis Virus ◽  
Diagnostic Methods ◽  
Lower Sensitivity ◽  
Dairy Goats ◽  
Caprine Arthritis Encephalitis Virus ◽  
Viral Culture

AbstractFor preventive and control strategies of Caprine Arthritis Encephalitis Virus (CAEV) infection in dairy goats, performance of the available diagnostic tests was described as one of the most important and necessary aspects. The study aimed at evaluating the diagnostic test performance, including PCR, ELISA and viral culture, for CAEV infection in dairy goats in Thailand. Blood samples of 29 dairy goats from five low- to medium-prevalence herds and one very low-prevalence herd were collected for PCR and ELISA methods. The performance of these two diagnostic methods was evaluated by comparing with cytopathic effects (CPE) in the co-cultivation of CAEV and primary synovial cells. Results indicated that sensitivity, specificity were, respectively, 69.6%, 100%, for PCR; and 95.7%, 83.3% for ELISA. The PCR assay tended to have lower sensitivity and higher specificity than ELISA. When multiple tests were applied, parallel testing provided sensitivity and specificity of 98.7% and 83.3%, while series testing showed sensitivity and specificity of 66.6% and 100% respectively. These results indicated that combination of ELISA and PCR provided some advantages and possibly offered optimal methods to detect CAEV-infected goats. Kappa value of the agreement between PCR and ELISA test was 0.34, indicating fair agreement. Regarding the possibility of antigenic variation between CAEV strains used in both PCR and ELISA assays, the actual circulating CAEV strain should be reviewed in order to develop and enhance the diagnostic tests using the CAE viral antigens derived from specific local strains of Thailand.

Chronic Wasting Disease Options of Surveillance in Carpathian Cervids: from the Identification of Characteristic Microscopic Pathologic Changes to Prionic Seeded Conversion and Amplification Assays

2001 ◽  
Vol 71 (3) ◽  
pp. 480-486
Author(s):  
Florica Barbuceanu ◽  
Stelian Baraitareanu ◽  
Stefania-Felicia Barbuceanu ◽  
Gabriel Predoi
Keyword(s):  
Enzyme Immunoassay ◽  
Diagnostic Tests ◽  
Chronic Wasting Disease ◽  
Rapid Diagnostic Tests ◽  
Diagnostic Methods ◽  
Wasting Disease ◽  
Western Immunoblotting ◽  
Negative Results ◽  
Antigen Capture ◽  
Confirmatory Tests

This paper describes the current diagnostic methods of Chronic Wasting Disease (CWD) in cervides used between 2013 and 2017 in Romania. The active surveillance of CWD involves the targeted groups screening by using rapid diagnostic tests (e.g., antigen capture enzyme immunoassay). If the first test does not provide certain negative results, then the confirmatory methods have been used, i.e. histopathology, immunohistochemistry and Western immunoblotting. These tests did not lead to the detection of CWD prions (PrPCWD) in Romania. This may be due to the absence or insufficient quantity of PrPCWD in samples, below the threshold of confirmatory tests.

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