Genomic Characterization of Listeria monocytogenes Isolated From Ready-to-Eat Meat and Meat Processing Environments in Poland

ABSTRACT This study was focused on characterization of the genetic diversity of Listeria monocytogenes isolated from packed fresh rabbit meat obtained from one producer via retail outlets. The partial aim was to compare the characteristics of a suspect persistent strain with strains from human cases. The occurrence of L. monocytogenes in vacuum-packed rabbit meat was monitored during 2013 to 2016. All strains were characterized by serotyping, pulsed-field gel electrophoresis, and multilocus sequence typing (MLST). Selected strains, which represented each year, were analyzed using the whole genome sequencing method. L. monocytogenes was detected in 21 (38%) of 56 originally packed rabbit meat samples from one food producer during the whole monitored period. All strains showed the identical serotype (1/2a), AscI/ApaI pulsotype (735/2), and sequence type (ST451). The clonal similarity of strains from rabbit meat was also confirmed on the basis of core genome MLST (on 1,701 loci). This fact suggests the occurrence of a suspect persistent strain in the meat processing plant. Results of core genome MLST enabled us to unambiguously exclude rabbit meat as a source of listeriosis in humans caused by the indistinguishable AscI/ApaI pulsotype and sequence type, although all strains carried all genes important for the virulence of L. monocytogenes. No specific genes that may be associated with its persistence in the food processing environment were detected among the tested strains of ST451. HIGHLIGHTS

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Phenotypic, Proteomic, and Genomic Characterization of a Putative ABC-Transporter Permease Involved in Listeria monocytogenes Biofilm Formation

Foodborne Pathogens and Disease ◽

10.1089/fpd.2010.0697 ◽

2011 ◽

Vol 8 (4) ◽

pp. 495-501 ◽

Cited By ~ 15

Author(s):

Xinna Zhu ◽

Weibing Liu ◽

René Lametsch ◽

Frank Aarestrup ◽

Chunlei Shi ◽

...

Keyword(s):

Listeria Monocytogenes ◽

Abc Transporter ◽

Biofilm Formation ◽

Genomic Characterization

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Characterization of Listeria monocytogenes Originating from the Spanish Meat-Processing Chain

Foods ◽

10.3390/foods8110542 ◽

2019 ◽

Vol 8 (11) ◽

pp. 542 ◽

Cited By ~ 2

Author(s):

Rosa Capita ◽

Amanda Felices-Mercado ◽

Camino García-Fernández ◽

Carlos Alonso-Calleja

Keyword(s):

Listeria Monocytogenes ◽

Clinical Importance ◽

Maximum Growth ◽

Maximum Growth Rate ◽

Meat Processing ◽

Hygiene Practices ◽

Adhesion Ability ◽

Listeria Ivanovii ◽

Good Hygiene

Using agglutination techniques, 118 Listeria monocytogenes isolates from red meat and poultry were serotyped. Strains were ascribed to the serotypes 4b/4e (44.1% of the strains), 1/2 (a, b or c; 28.0%), 4c (6.8%), 4d/4e (5.9%) and 3 (a, b or c; 2.5%). Among these are the serotypes most frequently involved in cases of human listeriosis. The susceptibility of 72 strains to 26 antibiotics of clinical importance was determined by disc diffusion (Clinical and Laboratory Standards Institute; CLSI). High levels of resistance were observed to cefoxitin (77.8% of the strains showed resistance), cefotaxime (62.5%), cefepime (73.6%), and nalidixic acid (97.2%), nitrofurantoin (51.4%) and oxacillin (93.1%). Less than 3% of the strains showed resistance to the antibiotic classes used in human listeriosis therapy (i.e., ampicillin, gentamicin, rifampicin, chloramphenicol, enrofloxacin, vancomycin, trimethoprim-sulfamethoxazole, erythromycin, and tetracycline). The influence of species and serotype on the growth kinetics (modified Gompertz equation) and on the adhesion ability (crystal violet staining) of nine isolates of L. monocytogenes (serotypes 1/2a, 1/2b, 1/2c, 3a, 3b, 3c, 4a, 4b, and 4d), and one strain of Listeria ivanovii were investigated. The maximum growth rate (ΔOD420-580/h) varied between 0.073 ± 0.018 (L. monocytogenes 1/2a) and 0.396 ± 0.026 (L. monocytogenes 4b). The isolates of L. monocytogenes belonging to serotypes 3a and 4a, as well as L. ivanovii, showed a greater (p < 0.05) biofilm-forming ability than did the remaining strains, including those that belong to the serotypes commonly implied in human listeriosis (1/2a, 1/2b, 1/2c and 4b). The need for training in good hygiene practices during the handling of meat and poultry is highlighted to reduce the risk of human listeriosis.

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Genomic characterization of the most barotolerant Listeria monocytogenes RO15 strain compared to reference strains used to evaluate food high pressure processing

BMC Genomics ◽

10.1186/s12864-020-06819-0 ◽

2020 ◽

Vol 21 (1) ◽

Author(s):

Ilhan Cem Duru ◽

Margarita Andreevskaya ◽

Pia Laine ◽

Tone Mari Rode ◽

Anne Ylinen ◽

...

Keyword(s):

High Pressure ◽

Listeria Monocytogenes ◽

High Pressure Processing ◽

Genomic Characterization ◽

Reference Strains

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Genomic Characterization of Clinical Listeria monocytogenes Isolates in Beijing, China

Frontiers in Microbiology ◽

10.3389/fmicb.2021.751003 ◽

2021 ◽

Vol 12 ◽

Author(s):

Xiaoai Zhang ◽

Yuzhu Liu ◽

Penghang Zhang ◽

Yanlin Niu ◽

Qian Chen ◽

...

Keyword(s):

Listeria Monocytogenes ◽

Common Core ◽

Core Genome ◽

The United States ◽

Multidrug Resistant ◽

Human Pathogen ◽

Genomic Characterization ◽

Beijing China ◽

Insight Into

Listeria monocytogenes is a foodborne human pathogen that affects public health worldwide. Whole-genome sequencing (WGS) can classify L. monocytogenes isolates and identify virulence islands and resistance genes potentially influencing infectivity. Herein, WGS was used to assess 151 L. monocytogenes isolates from 120 cases of clinical infection in Beijing, China, between 2014 and 2018. Most isolates were either serogroup 1/2a,3a or serogroup 1/2b,3b,7, with 25 multilocus sequence typing (MLST) types (STs) represented, of which ST8, ST87, and ST5 were the most common. Core-genome MLST (cgMLST) grouped the 151 isolates into 116 cgMLST types. The discriminatory power of cgMLST was greater than other subtypes, revealing that isolates from the same patient were highly related (only differing at one allele). Eighty-six isolates formed 30 complexes with ≤ 7 cgMLST alleles between neighboring isolates, suggesting possible outbreaks. Compared with isolates in the United States, ST8, ST121, ST619, ST87, and ST155 isolates were grouped into unified clades. All 151 isolates were positive for common virulence-associated loci, and 26 lineage I isolates harbored the pathogenicity island 3 (LIPI-3) locus, while 42 lineage I isolates harbored the complete LIPI-4 locus. Eleven ST619 isolates had both LIPI-3 and LIPI-4. Among the 151 isolates, 13 were resistant to at least one antibiotic, and no multidrug-resistant isolates were identified. Resistance phenotypes correlated with genotypes, apart from two meropenem resistance isolates. The findings provided insight into the nature of L. monocytogenes strains currently causing clinical disease in Beijing, and WGS analysis indicated possible outbreaks.

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