scholarly journals Inexpensive Apparatus for High-Quality Imaging of Microbial Growth on Agar Plates

2021 ◽  
Vol 12 ◽  
Author(s):  
Parker Smith ◽  
Martin Schuster

The ability to capture images of results or processes is an important tool in the biologist’s tool kit. In microbiology, capturing high-quality images of microbial growth on agar plates is difficult due to the reflective surface of the plates and limitations in common photography techniques. Equipment is available to overcome these challenges, but acquisition costs are high. We have developed and tested an inexpensive and efficient apparatus for high-quality imaging of microbial colonies. The imaging box, as we have named the apparatus, is designed to eliminate glare and reduce reflections on the surface of the plate while providing uniform, diffuse light from all sides. The imaging box was used to capture hundreds of images in research and teaching lab settings.

2016 ◽  
Vol 71 (5) ◽  
pp. 988-995 ◽  
Author(s):  
Patrick D. Barnett ◽  
S. Michael Angel

A spatial heterodyne Raman spectrometer (SHRS) with millimeter-sized optics has been coupled with a standard cell phone camera as a detector for Raman measurements. The SHRS is a dispersive-based interferometer with no moving parts and the design is amenable to miniaturization while maintaining high resolution and large spectral range. In this paper, a SHRS with 2.5 mm diffraction gratings has been developed with 17.5 cm−1 theoretical spectral resolution. The footprint of the SHRS is orders of magnitude smaller than the footprint of charge-coupled device (CCD) detectors typically employed in Raman spectrometers, thus smaller detectors are being explored to shrink the entire spectrometer package. This paper describes the performance of a SHRS with 2.5 mm wide diffraction gratings and a cell phone camera detector, using only the cell phone’s built-in optics to couple the output of the SHRS to the sensor. Raman spectra of a variety of samples measured with the cell phone are compared to measurements made using the same miniature SHRS with high-quality imaging optics and a high-quality, scientific-grade, thermoelectrically cooled CCD.


Author(s):  
Zulkarnain Hanafi ◽  
Chee Kiong Tong

The paper will cover all aspects of the change journey: engaging with relevant stakeholders, the recruitment and retention of high quality faculty members, the review and revision of the curriculum, improving the quality and quantity of research output and publications, developing centers of research excellence, raising the level of funding for both research and teaching, expanding the number of graduate students, developing an eminent visiting professors' program, the internationalization of the university, strengthening governance and administration and raising the international profile of the university. It will set out, in detail, the strategies and processes that were developed to realize the vision, as well as the challenges and problems encountered, and steps taken to address these challenges and problems. Mistakes were made along the way and the lessons that can be learnt for any university that aims to be involved in the ranking exercises.


2001 ◽  
Vol 205 ◽  
pp. 430-431
Author(s):  
Sergey Likhachev

This presentation focuses on the problems associated with obtaining high quality images from a high orbiting space VLBI (SVLBI) mission. SVLBI intensifies and magnifies all imaging problems, making these problems clearer to understand, though much harder to solve.


1997 ◽  
Author(s):  
Makoto M. Miyahara ◽  
Shouji Ohtsuka ◽  
Shuji Taniho ◽  
V. Ralph Algazi

2019 ◽  
Vol 35 (10) ◽  
pp. 1893-1901 ◽  
Author(s):  
Gabriella Captur ◽  
Ilaria Lobascio ◽  
Yang Ye ◽  
Veronica Culotta ◽  
Redha Boubertakh ◽  
...  

1987 ◽  
Vol 51 (359) ◽  
pp. 21-31 ◽  
Author(s):  
I. C. Freestone ◽  
A. P. Middleton

AbstractThe modern analytical SEM, which can provide high-quality imaging facilities together with quantitative elemental analysis using an energy-dispersive spectrometer, is finding wide application in the investigation of archaeological problems. Many of these investigations involve the study of silicate and carbonate-based artefacts which may be relatively unmodified from their original geological parent raw materials so that mineralogically based interpretations are often appropriate. In this paper we present a series of examples illustrating the role of the analytical SEM in the mineralogical investigation of archaeological problems, including the characterization and provenancing of geological raw materials, the elucidation of the processes used to transform those raw materials into useful objects and the recognition and characterization of changes which archaeological artefacts may have undergone during burial or during storage.


2019 ◽  
Vol 58 (13) ◽  
pp. D50 ◽  
Author(s):  
Jian Zhao ◽  
Mostafa Peysokhan ◽  
Jose Enrique Antonio-Lopez ◽  
Yangyang Sun ◽  
Behnam Abaie ◽  
...  

2013 ◽  
Vol 694-697 ◽  
pp. 1001-1007 ◽  
Author(s):  
Om Krishna Suwal ◽  
Anjli Sharma ◽  
Young Bok Lee ◽  
Tae Sik Oh ◽  
Dae Wook Kim ◽  
...  

Microcolumn, a miniaturized electron optical system, is a powerful tool in manipulating electron beam for maskless direct e-beam lithography and miniaturized low voltage SEM for surface inspection, testing, and metrology. The basic parts of microcolumn are electron emitter, source lens, deflector, and Einzel lens. There are still several challenges in optimization of each component for better performance of microcolumn for aberration-free high quality imaging and large field of view. For the improvement of microcolumn, we developed a fabrication technique of making thin electrostatic lens using micro-electromechanical systems (MEMS) processes. Two types of microcolumns have been assembled by varying the spaces between Einzel lens-electrodes, and their performance have been evaluated for the comparison. The scan range is found to be increased with reducing the gap between the lenses and increasing working distance. The effect of the spatial gap on the scan range and image is analyzed through simulation study on the electric potential and field strength.


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