scholarly journals Corrigendum: In vivo Hippocampal Serotonin Dynamics in Male and Female Mice: Determining Effects of Acute Escitalopram Using Fast Scan Cyclic Voltammetry

2019 ◽  
Vol 13 ◽  
Author(s):  
Rachel A. Saylor ◽  
Melinda Hersey ◽  
Alyssa West ◽  
Anna Marie Buchanan ◽  
Shane N. Berger ◽  
...  
Keyword(s):  
Cyclic Voltammetry ◽  
Fast Scan Cyclic Voltammetry ◽  
Male And Female ◽  
Female Mice

scholarly journals In vivo Hippocampal Serotonin Dynamics in Male and Female Mice: Determining Effects of Acute Escitalopram Using Fast Scan Cyclic Voltammetry

2019 ◽  
Vol 13 ◽  
Author(s):  
Rachel A. Saylor ◽  
Melinda Hersey ◽  
Alyssa West ◽  
Anna Marie Buchanan ◽  
Shane N. Berger ◽  
...  
Keyword(s):  
Cyclic Voltammetry ◽  
Fast Scan Cyclic Voltammetry ◽  
Male And Female ◽  
Female Mice

Striatal low-threshold spiking interneurons locally gate dopamine during learning

2020 ◽  
Author(s):  
Elizabeth N. Holly ◽  
M. Felicia Davatolhagh ◽  
Rodrigo A. España ◽  
Marc V. Fuccillo
Keyword(s):  
Cyclic Voltammetry ◽  
Dopamine Release ◽  
Striatal Dopamine ◽  
Operant Learning ◽  
Fast Scan Cyclic Voltammetry ◽  
Directed Learning ◽  
Low Threshold ◽  
Dorsomedial Striatum

Low-threshold spiking interneurons (LTSIs) in the dorsomedial striatum are potent modulators of goal-directed learning. Here, we uncover a novel function for LTSIs in locally and directly gating striatal dopamine, using in vitro fast scan cyclic voltammetry as well as in vivo GRAB-DA sensor imaging and pharmacology during operant learning. We demonstrate that LTSIs, acting via GABAB signaling, attenuate dopamine release, thereby serving as local coordinators of striatal plasticity.

KEAMANAN TEH GAHARU (Aquilaria malaccencis Lamk ) DARI POHON INDUKSI TERHADAP TOKSIK ORAL

2018 ◽  
Vol 13 (1) ◽  
pp. 1-11
Author(s):  
Ridwanti Batubara ◽  
Surjanto Surjanto ◽  
Marsen Purba
Keyword(s):  
Antioxidant Activity ◽  
Research Method ◽  
Male And Female ◽  
Female Mice ◽  
All Treatment

Leaves aloes (Aquilaria malaccencis Lamk) used the farmer  as a drink that in pour (tea). The result of that tea aloes have a very strong antioxidant activity. The problem are the aleo tea from tree induction safety of consume. This study aims to determine the symptoms of toxic  posed of product tea aloes induction.  The research method refers to the Guidance of Toxicity Non-Clinic Test in Vivo, Badan POM RI, 2011. Results showed that not found toxic symptoms in all treatment of male and female  mice, safe for consumption.

scholarly journals Design Choices for Next-Generation Neurotechnology Can Impact Motion Artifact in Electrophysiological and Fast-Scan Cyclic Voltammetry Measurements

Micromachines ◽  
2018 ◽  
Vol 9 (10) ◽  
pp. 494 ◽  
Author(s):  
Evan Nicolai ◽  
Nicholas Michelson ◽  
Megan Settell ◽  
Seth Hara ◽  
James Trevathan ◽  
...  
Keyword(s):  
Cyclic Voltammetry ◽  
Electrode Materials ◽  
Motion Artifact ◽  
Next Generation ◽  
Fast Scan Cyclic Voltammetry ◽  
Flexible Electrodes ◽  
In Vivo Experiments ◽  
Electrophysiological Recordings

Implantable devices to measure neurochemical or electrical activity from the brain are mainstays of neuroscience research and have become increasingly utilized as enabling components of clinical therapies. In order to increase the number of recording channels on these devices while minimizing the immune response, flexible electrodes under 10 µm in diameter have been proposed as ideal next-generation neural interfaces. However, the representation of motion artifact during neurochemical or electrophysiological recordings using ultra-small, flexible electrodes remains unexplored. In this short communication, we characterize motion artifact generated by the movement of 7 µm diameter carbon fiber electrodes during electrophysiological recordings and fast-scan cyclic voltammetry (FSCV) measurements of electroactive neurochemicals. Through in vitro and in vivo experiments, we demonstrate that artifact induced by motion can be problematic to distinguish from the characteristic signals associated with recorded action potentials or neurochemical measurements. These results underscore that new electrode materials and recording paradigms can alter the representation of common sources of artifact in vivo and therefore must be carefully characterized.

scholarly journals Cytochrome P450 Genes Are Differentially Expressed in Female and Male Hepatocyte Retinoid X Receptor α-Deficient Mice

Endocrinology ◽  
2003 ◽  
Vol 144 (6) ◽  
pp. 2311-2318 ◽  
Author(s):  
Yan Cai ◽  
Tiane Dai ◽  
Yan Ao ◽  
Tamiko Konishi ◽  
Kuang-Hsiang Chuang ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Negative Impact ◽  
Retinoid X Receptor ◽  
Differentially Expressed ◽  
Male Mice ◽  
Wild Type ◽  
Male And Female ◽  
Female Mice ◽  
Deficient Mice

Abstract To study the functional role of retinoid X receptor α (RXRα) in hepatocytes, hepatocyte RXRα-deficient mice have been established. Characterization has been performed on male mice. In this paper, we show that the expression of CYP450 genes is differentially expressed in male and female hepatocyte RXRα-deficient mice; male mice have reduced expression of cytochrome P450 (CYP) CYP4A, CYP3A, and CYP2B mRNAs, but females do not exhibit such phenotypes. To examine the hormonal effects on this sexual dimorphic phenotype, male and female mice were subjected to 17β-estradiol and 5α-dihydrotestosterone (DHT) treatment, respectively, and then the expression of the CYP450 genes was studied. Estradiol had no effect on protecting the hepatocyte RXRα-deficient mice from reduced expression of the CYP450 genes. In contrast, DHT induced hepatocyte RXRα-deficient female mice, but not wild-type female mice, to have the reduced expression of CYP450 mRNAs. In addition, castration prevented the mutant male mice from exhibiting reduced expression of CYP450 mRNAs. wild-type and mutant mouse livers from both genders express androgen receptors (ARs). By transient transfection, DHT-AR could inhibit RXRα-mediated transcription. Furthermore, by transfection and coimmunoprecipitation, RXR can interact with AR in vivo. These data suggest that testosterone has a negative impact on retinoid signaling when the level of RXRα is low, which may in turn reduce the expression of the CYP450 genes.

scholarly journals Development of the Wireless Instantaneous Neurotransmitter Concentration System for intraoperative neurochemical monitoring using fast-scan cyclic voltammetry

2009 ◽  
Vol 111 (4) ◽  
pp. 712-723 ◽  
Author(s):  
Jonathan M. Bledsoe ◽  
Christopher J. Kimble ◽  
Daniel P. Covey ◽  
Charles D. Blaha ◽  
Filippo Agnesi ◽  
...  
Keyword(s):  
Cyclic Voltammetry ◽  
Carbon Fiber ◽  
Flow Injection ◽  
High Frequency ◽  
Dopamine Release ◽  
Functional Neurosurgery ◽  
Fast Scan Cyclic Voltammetry ◽  
Stimulation Of

Object Emerging evidence supports the hypothesis that modulation of specific central neuronal systems contributes to the clinical efficacy of deep brain stimulation (DBS) and motor cortex stimulation (MCS). Real-time monitoring of the neurochemical output of targeted regions may therefore advance functional neurosurgery by, among other goals, providing a strategy for investigation of mechanisms, identification of new candidate neurotransmitters, and chemically guided placement of the stimulating electrode. The authors report the development of a device called the Wireless Instantaneous Neurotransmitter Concentration System (WINCS) for intraoperative neurochemical monitoring during functional neurosurgery. This device supports fast-scan cyclic voltammetry (FSCV) at a carbon-fiber microelectrode (CFM) for real-time, spatially and chemically resolved neurotransmitter measurements in the brain. Methods The FSCV study consisted of a triangle wave scanned between −0.4 and 1 V at a rate of 300 V/second and applied at 10 Hz. All voltages were compared with an Ag/AgCl reference electrode. The CFM was constructed by aspirating a single carbon fiber (r = 2.5 μm) into a glass capillary and pulling the capillary to a microscopic tip by using a pipette puller. The exposed carbon fiber (that is, the sensing region) extended beyond the glass insulation by ~ 100 μm. The neurotransmitter dopamine was selected as the analyte for most trials. Proof-of-principle tests included in vitro flow injection and noise analysis, and in vivo measurements in urethane-anesthetized rats by monitoring dopamine release in the striatum following high-frequency electrical stimulation of the medial forebrain bundle. Direct comparisons were made to a conventional hardwired system. Results The WINCS, designed in compliance with FDA-recognized consensus standards for medical electrical device safety, consisted of 4 modules: 1) front-end analog circuit for FSCV (that is, current-to-voltage transducer); 2) Bluetooth transceiver; 3) microprocessor; and 4) direct-current battery. A Windows-XP laptop computer running custom software and equipped with a Universal Serial Bus–connected Bluetooth transceiver served as the base station. Computer software directed wireless data acquisition at 100 kilosamples/second and remote control of FSCV operation and adjustable waveform parameters. The WINCS provided reliable, high-fidelity measurements of dopamine and other neurochemicals such as serotonin, norepinephrine, and ascorbic acid by using FSCV at CFM and by flow injection analysis. In rats, the WINCS detected subsecond striatal dopamine release at the implanted sensor during high-frequency stimulation of ascending dopaminergic fibers. Overall, in vitro and in vivo testing demonstrated comparable signals to a conventional hardwired electrochemical system for FSCV. Importantly, the WINCS reduced susceptibility to electromagnetic noise typically found in an operating room setting. Conclusions Taken together, these results demonstrate that the WINCS is well suited for intraoperative neurochemical monitoring. It is anticipated that neurotransmitter measurements at an implanted chemical sensor will prove useful for advancing functional neurosurgery.

scholarly journals Fast-scan cyclic voltammetry demonstrates that L-DOPA produces dose-dependent, regionally selective bimodal effects on striatal dopamine kinetics in vivo

2016 ◽  
Vol 136 (6) ◽  
pp. 1270-1283 ◽  
Author(s):  
Rashed Harun ◽  
Kristin M. Hare ◽  
Elizabeth M. Brough ◽  
Miranda J. Munoz ◽  
Christine M. Grassi ◽  
...  
Keyword(s):  
Cyclic Voltammetry ◽  
Striatal Dopamine ◽  
Fast Scan Cyclic Voltammetry ◽  
Dose Dependent ◽  
Kinetics In Vivo

Reducing the Sampling Rate of Biochemical Measurements Using Fast-Scan Cyclic Voltammetry for In Vivo Applications

2014 ◽  
Vol 14 (9) ◽  
pp. 2975-2980 ◽  
Author(s):  
Alison N. Amos ◽  
James G. Roberts ◽  
Lingjiao Qi ◽  
Leslie A. Sombers ◽  
Gregory S. McCarty
Keyword(s):  
Cyclic Voltammetry ◽  
Sampling Rate ◽  
Fast Scan Cyclic Voltammetry ◽  
Biochemical Measurements
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