Antibacterial Activity and Mechanism of Action of Aspidinol Against Multi-Drug-Resistant Methicillin-Resistant Staphylococcus aureus

One new and seven known diterpenes were identified from an antibacterial chromatographic fraction of Taxodium ascendens. Of these, demethylcryptojaponol (2), 6-hydroxysalvinolone (3), hydroxyferruginol (4), and hinokiol (5) demonstrated potent activity against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA). These compounds represent a class of synthetically accessible compounds that could be further developed for treatment of drug-resistant bacterial infections.

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Terpenes from Zingiber montanum and Their Screening against Multi-Drug Resistant and Methicillin Resistant Staphylococcus aureus

Molecules ◽

10.3390/molecules24030385 ◽

2019 ◽

Vol 24 (3) ◽

pp. 385 ◽

Cited By ~ 1

Author(s):

Holly Siddique ◽

Barbara Pendry ◽

M. Mukhlesur Rahman

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Secondary Metabolites ◽

Mass Spectra ◽

Methicillin Resistant Staphylococcus Aureus ◽

Drug Resistant ◽

Two Dimensional ◽

Methicillin Resistant ◽

One Dimensional ◽

Nmr Data

Bioassay directed isolation of secondary metabolites from the rhizomes of Zingiber montanum (Fam. ‎Zingiberaceae) led to the isolation of mono-, sesqui-, and di-terpenes. The compounds were characterized as (E)-8(17),12-labdadiene-15,16-dial (1), zerumbol (2), zerumbone (3), buddledone A (4), furanodienone (5), germacrone (6), borneol (7), and camphor (8) by analysing one-dimensional (1D) (1H and 13C) and two-dimensional (2D) (COSY, HSQC, HMBC, and NOESY) NMR data and mass spectra. Among these terpenes, compounds 1 and 2 revealed potential antibacterial activity (minimum inhibitory concentrations (MIC) values 32–128 µg/mL; 0.145–0.291 mM)) against a series of clinical isolates of multi-drug resistant (MDR) and Methicillin resistant Staphylococcus aureus (MRSA).

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Antibacterial Activity of BSA-Capped Gold Nanoclusters against Methicillin-Resistant Staphylococcus aureus (MRSA) and Vancomycin-Intermediate Staphylococcus aureus (VISA)

Journal of Nanomaterials ◽

10.1155/2019/4101293 ◽

2019 ◽

Vol 2019 ◽

pp. 1-7 ◽

Cited By ~ 3

Author(s):

Shu-Hua Kuo ◽

Chi-Sheng Chien ◽

Chun-Chi Wang ◽

Chi-Jen Shih

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bovine Serum Albumin ◽

Serum Albumin ◽

Bovine Serum ◽

Methicillin Resistant Staphylococcus Aureus ◽

Gold Nanoclusters ◽

Drug Resistant ◽

Fluorescence Image ◽

Methicillin Resistant

The emergence of drug-resistant pathogens and the abuse of antibiotics have posed dominant threats to society. In this study, we propose an antibacterial use of bovine serum albumin-capped gold nanoclusters (BSA-AuNCs) to address the issue. BSA-AuNCs have a great antibacterial activity against MRSA and VISA which is proved by time-killing curves (TKC). The possible antibacterial mechanisms of BSA-AuNCs against MRSA and VISA are confirmed by fluorescence image observation.

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ANTIBACTERIAL ACTIVITY OF PSIDIUM GUAJAVA LINN. STEM BARK EXTRACTS ON MULTI DRUG RESISTANT (MDR) COMMUNITY ACQUIRED METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (CA-MRSA)

Journal of Evolution of Medical and Dental Sciences ◽

10.14260/jemds/1127 ◽

2013 ◽

Vol 2 (33) ◽

pp. 6251-6260

Author(s):

Chibuike Ibe ◽

Ngozi Lucy Maduagwu

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Methicillin Resistant Staphylococcus Aureus ◽

Stem Bark ◽

Psidium Guajava ◽

Drug Resistant ◽

Methicillin Resistant

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Antibacterial activity of unifloral honeys against clinical isolates of methicillin-resistant Staphylococcus aureus

Planta Medica ◽

10.1055/s-0029-1234974 ◽

2009 ◽

Vol 75 (09) ◽

Author(s):

A Hannan ◽

MB Hussain ◽

M Absar

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Methicillin Resistant Staphylococcus Aureus ◽

Clinical Isolates ◽

Methicillin Resistant

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In vitro antibacterial activity of some spice extracts against methicillin-resistant Staphylococcus aureus isolated from nose of food handlers

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2020.12.03.412 ◽

2020 ◽

Vol 12 (03) ◽

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Methicillin Resistant Staphylococcus Aureus ◽

Food Handlers ◽

Methicillin Resistant ◽

In Vitro Antibacterial Activity

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A Molecular Insight into the Synergistic Mechanism of Nigella sativa (Black Cumin) with β-Lactam Antibiotics against Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

Applied Sciences ◽

10.3390/app11073206 ◽

2021 ◽

Vol 11 (7) ◽

pp. 3206

Author(s):

Lorina I. Badger-Emeka ◽

Promise Madu Emeka ◽

Hairul Islam M. Ibrahim

Keyword(s):

Staphylococcus Aureus ◽

Mechanism Of Action ◽

Methicillin Resistant Staphylococcus Aureus ◽

Nigella Sativa ◽

Synergistic Effects ◽

Diffusion Method ◽

Methicillin Resistant ◽

Cell Wall Disruption ◽

Time Kill ◽

Insight Into

Methicillin-resistant Staphylococcus aureus (MRSA) infection is detrimental to hospitalized patients. With diminishing choices of antibiotics and the worry about resistance to colistin in synergistic combined therapy, there are suggestions for the use of herbal derivatives. This investigation evaluated the synergistic effects of Nigella sativa (NS) in combination with beta-lactam (β-lactam) antibiotics on extreme drug-resistant (XDR) MRSA isolates. NS concentrations of 10, 7.5, 5.0, 2.5, 1.0, and 0.1 µg/mL, alone and in combination with β-lactam antibiotics, were used to determine the antimicrobial susceptibility of MRSA isolates by the well diffusion method. Time–kill assays were performed using a spectrophotometer, with time–kill curves plotted and synergism ascertained by the fractional inhibitory concentration (FIC). Scanning and transmission electron microscopy were used to gain insight into the mechanism of action of treated groups. Isolates were inhibited by the NS concentrations, with differences in the zones of inhibition being statistically insignificant at p < 0.05. There were statistically significant differences in the time–kill assay for the MRSA isolates. In addition, NS combined with augmentin showed better killing than oxacillin and cefuroxime. The mechanism of action shown by the SEM and TEM results revealed cell wall disruption, which probably created interference that led to bacterial lysis.

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