Long-Term Toxicity Study of Topical Administration of a Highly-Stable rh-aFGF Carbomer 940 Hydrogel in a Rabbit Skin Wound Model

Topical administration of powdered shells of the land snailMegalobulimus lopesiwas evaluated in Wistar rats for their healing activity in an excision wound model. The animals were distributed into three groups—G1 (control): no therapeutic intervention; G2 (vehicle controls): Lanette cream once daily; G3 (experimental animals): treated with powdered shells. Variables investigated were: wound area contraction, angiogenic activity, morphometric data, leukocytic inflammatory infiltrate, and total leukocyte count in peripheral blood. Thermogravimetric analysis and quantification and characterization of powdered shell proteins were also performed. Wound area on days 3, 7, and 14 was smaller in G3, besides presenting wound closure on day 21 for all these animals. Topical administration of the powdered shells also led to an increased number of vessels at the wound site, higher leukocyte counts in peripheral blood, and increased leukocytic inflammatory infiltrate. The results lend support to the southern Brazilian folk use ofM. lopesipowdered shells, as shown by the enhanced secondary-intention healing achieved with their topical administration to wounds in rats. Topical administration caused inflammatory response modulation, crucial to accelerating the healing process, the chronification of which increases the risks of wound contamination by opportunistic pathogens.

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The topical administration of rhEGF-loaded nanostructured lipid carriers (rhEGF-NLC) improves healing in a porcine full-thickness excisional wound model

Journal of Controlled Release ◽

10.1016/j.jconrel.2014.10.033 ◽

2015 ◽

Vol 197 ◽

pp. 41-47 ◽

Cited By ~ 52

Author(s):

Garazi Gainza ◽

Diego Celdran Bonafonte ◽

Beatriz Moreno ◽

José Javier Aguirre ◽

Francisco Borja Gutierrez ◽

...

Keyword(s):

Topical Administration ◽

Nanostructured Lipid Carriers ◽

Full Thickness ◽

Wound Model ◽

Excisional Wound

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Management of Missed Traumatic Achilles Tendon Rupture in a Pediatric Patient: A Case Report

Pediatric Traumatology Orthopaedics and Reconstructive Surgery ◽

10.17816/ptors77113 ◽

2021 ◽

Vol 9 (4) ◽

pp. 465-470

Author(s):

Saad Andaloussi

Keyword(s):

Achilles Tendon ◽

Tendon Rupture ◽

Achilles Tendon Rupture ◽

Tendon Repair ◽

Skin Wound ◽

Plantaris Tendon ◽

First Case ◽

Tendon Ruptures ◽

Post Traumatic

BACKGROUND: Missed traumatic Achilles tendon ruptures in children are rarely reported in the literature. Various techniques have been described to reconstruct delayed Achilles tendon ruptures for adults, but the long-term consequences in the growing child are unknown. CLINICAL CASE: The article presents a clinical observation of a 8-year-old girl with missed rupture of the Achilles tendon operated 7 weeks after the trauma by end-to-end Kessler-type sutures augmented with the plantaris tendon. At 2-year follow-up, the patient was completely asymptomatic. DISCUSSION: A review of the literature shows that this is the third neglected pediatric case of post-traumatic Achilles tendon rupture. The first case concerns a 10-year-old boy treated successfully six weeks after the traumat by open surgical repair using the Bunnell sutures technique. The second patient was a 7-year-old girl, she was operated 8 weeks after the trauma with a termino-terminal tenorrhaphy using the Bunnell technique augmented with the plantaris tendon. CONCLUSIONS: Using the plantaris tendon to reinforce the Achilles tendon repair offers satisfactory results with minimal morbidity. Prognosis depends on the extent of tendon defect which determines the long-term functional outcome. Any skin wound that sits on the back of the leg requires a systematic and careful physical examination to check the integrity of the Achilles tendon.

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Systemic and topical administration of spermidine accelerates skin wound healing

10.21203/rs.3.rs-111107/v1 ◽

2020 ◽

Author(s):

Daisuke Ito ◽

Hiroyasu Ito ◽

Takayasu Ideta ◽

Ayumu Kanbe ◽

Soranobu Ninomiya ◽

...

Keyword(s):

Cell Proliferation ◽

Wound Healing ◽

Wound Repair ◽

Healing Process ◽

Topical Administration ◽

Skin Wound ◽

Wound Healing Process ◽

Skin Wound Healing

Abstract Background The skin wound healing process is regulated by various cytokines, chemokines, and growth factors. Recent reports have demonstrated that spermine/spermidine (SPD) promote wound healing through urokinase-type plasminogen activator (uPA)/uPA receptor (uPAR) signaling in vitro. Here, we investigated whether the systemic and topical administration of SPD would accelerate the skin wound-repair process in vivo.Methods A skin wound repair model was established using C57BL/6 J mice. SPD was mixed with white petrolatum for topical administration. For systemic administration, SPD mixed with drinking water was orally administered. Changes in wound size over time were calculated using digital photography.Results Systemic and topical SPD treatment significantly accelerated skin wound healing. The administration of SPD promoted the uPA/uPAR pathway in wound sites. Moreover, topical treatment with SPD enhanced the expression of IL-6 and TNF-α in wound sites. Scratch and cell proliferation assays revealed that SPD administration accelerated scratch wound closure and cell proliferation in vitro.Conclusion These results indicate that treatment with SPD promotes skin wound healing through activation of the uPA/uPAR pathway and induction of the inflammatory response in wound sites. The administration of SPD might contribute to new effective treatments to accelerate skin wound healing.

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Adenosine diphosphate contributes to wound healing in diabetic mice through P2Y1 and P2Y12 receptors activation

10.1101/2020.10.22.350785 ◽

2020 ◽

Author(s):

Paula A. Borges ◽

Ingrid Waclawiak ◽

Janaína L. Georgii ◽

Janaína F. Barros ◽

Vanderlei S. Fraga-Junior ◽

...

Keyword(s):

Wound Healing ◽

Inflammatory Diseases ◽

Purinergic Signaling ◽

Adenosine Diphosphate ◽

Treg Cells ◽

Skin Wound ◽

Diabetic Patients ◽

Dependent Manner ◽

Keratinocyte Proliferation ◽

Wound Model

AbstractSeveral studies have shown the importance of purinergic signaling in various inflammatory diseases. In diabetes mellitus, there is an increase in the activity of some nucleotidases suggesting that this signaling may be affected in the diabetic skin. Thus, the aim of our study was to investigate the effect of ADP on wound healing in diabetic skin. Swis and C57BL/6 mice were pharmacologic induced to type 1 diabetes and submitted to a full-thickness excisional wound model to evaluate the effect of ADP as a topic treatment. Adenosine diphosphate accelerated cutaneous wound healing, improved the new tissue formation, and increased collagen deposit by positively modulating P2Y1 and P2Y12 and TGF-β production. In parallel, ADP reduced reactive oxygen species production and TNF-α levels, while increased IFNγ, IL-10 and IL-13 levels in the skin. Also, ADP induced the migration of neutrophils, eosinophils, mast cells, TCRγ4+, and TCRγ5+ cells while reduced Treg cells towards the lesion at day 7. In accordance, ADP increased the proliferation and migration of fibroblast, induced myofibroblast differentiation and keratinocyte proliferation in a P2Y12-dependent manner. We provide the first evidence of ADP acting as a potent mediator on skin wound resolution and a possible therapeutic approach for diabetic patients worldwide.

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Ischemic Non-Healing Skin Wound Model: Cell Death and Wound Healing Mechanisms

Sovremennye tehnologii v medicine ◽

10.17691/stm2018.10.4.08 ◽

2018 ◽

Vol 10 (4) ◽

pp. 69

Author(s):

E.I. Morgun ◽

O.S. Rogovaya ◽

E.A. Vorotelyak

Keyword(s):

Wound Healing ◽

Cell Death ◽

Skin Wound ◽

Wound Model ◽

Model Cell

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Sundew-Inspired Adhesive Hydrogel Threads through Reversible Complexation of Polyphenol and Boronic Acid

Applied Sciences ◽

10.3390/app11188591 ◽

2021 ◽

Vol 11 (18) ◽

pp. 8591

Author(s):

Jae Hyuk Choi ◽

Donghee Son ◽

Mikyung Shin

Keyword(s):

Boronic Acid ◽

Skin Wound ◽

Body Fluids ◽

The Body ◽

Wound Dressings ◽

Suture Materials ◽

Wound Site ◽

Long Term Stability ◽

State Dependent

Adhesive hydrogels have been utilized as tissue sealants, hemostatic agents, and wound dressings, with the aim of replacing conventional sutures. To prevent immune response and serious inflammation from those hydrogels after sealing, natural biocompatible polysaccharides are widely used as a component of the hydrogels. However, the weak mechanical strength, insufficient adhesiveness, and rapid dissociation of the hydrogels necessitates additional suturing at the wound site. In this study, we report on a solid polysaccharide thread reversibly crosslinked with boronic acid-polyphenol complexation and its adhesive gelation for complete tissue sealing without additional suturing. The polysaccharide adhesive suture threads demonstrated long-term stability, which is useful when used for skin wound suturing. Specifically, their robust adhesion property occurred as soon as the threads were soaked, attaining a swollen hydrogel state, dependent on the presence of body fluids after suturing. Moreover, the pH of the body fluids affects the viscoelasticity and adhesiveness of the hydrogels in order to ensure a tight sealing. Therefore, we expect that these pH-responsive adhesive threads would be promising for the development of on-demand functional suture materials.

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