scholarly journals Root Zone Cooling and Exogenous Spermidine Root-Pretreatment Promoting Lactuca sativa L. Growth and Photosynthesis in the High-temperature Season

2016 ◽  
Vol 7 ◽  
Author(s):  
Jin Sun ◽  
Na Lu ◽  
Hongjia Xu ◽  
Toru Maruo ◽  
Shirong Guo
Keyword(s):  
High Temperature ◽  
Lactuca Sativa ◽  
Root Zone ◽  
Lactuca Sativa L ◽  
Exogenous Spermidine

scholarly journals Phosphoproteomic analysis of lettuce (Lactuca sativa L.) reveals starch and sucrose metabolism functions during bolting induced by high temperature

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0244198
Author(s):  
Xiaoxiao Qin ◽  
Panpan Li ◽  
Shaowei Lu ◽  
Yanchuan Sun ◽  
Lifeng Meng ◽  
...  
Keyword(s):  
High Temperature ◽  
High Temperatures ◽  
Lactuca Sativa ◽  
Molecular Mechanisms ◽  
Soluble Sugar ◽  
High Temperature Stress ◽  
Temperature Treatment ◽  
Sucrose Metabolism ◽  
High Temperature Treatment ◽  
Lactuca Sativa L

High temperatures induce early bolting in lettuce (Lactuca sativa L.), which decreases both quality and production. However, knowledge of the molecular mechanism underlying high temperature promotes premature bolting is lacking. In this study, we compared lettuce during the bolting period induced by high temperatures (33/25 °C, day/night) to which raised under controlled temperatures (20/13 °C, day/night) using iTRAQ-based phosphoproteomic analysis. A total of 3,814 phosphorylation sites located on 1,766 phosphopeptides from 987 phosphoproteins were identified after high-temperature treatment,among which 217 phosphoproteins significantly changed their expression abundance (116 upregulated and 101 downregulated). Most phosphoproteins for which the abundance was altered were associated with the metabolic process, with the main molecular functions were catalytic activity and transporter activity. Regarding the functional pathway, starch and sucrose metabolism was the mainly enriched signaling pathways. Hence, high temperature influenced phosphoprotein activity, especially that associated with starch and sucrose metabolism. We suspected that the lettuce shorten its growth cycle and reduce vegetative growth owing to changes in the contents of starch and soluble sugar after high temperature stress, which then led to early bolting/flowering. These findings improve our understanding of the regulatory molecular mechanisms involved in lettuce bolting.

scholarly journals Quantitative Proteomics Analysis of Lettuce (Lactuca sativa L.) Reveals Molecular Basis-Associated Auxin and Photosynthesis with Bolting Induced by High Temperature

2018 ◽  
Vol 19 (10) ◽  
pp. 2967 ◽  
Author(s):  
Jing-Hong Hao ◽  
Li-Li Zhang ◽  
Pan-Pan Li ◽  
Yan-Chuan Sun ◽  
Jian-Ke Li ◽  
...  
Keyword(s):  
High Temperature ◽  
Lactuca Sativa ◽  
Molecular Basis ◽  
Tryptophan Metabolism ◽  
Proteomics Analysis ◽  
Differential Abundance ◽  
Control Temperature ◽  
Lactuca Sativa L ◽  
Iaa Biosynthesis ◽  
Abundance Proteins

Bolting is a key process in the growth and development of lettuce (Lactuca sativa L.). A high temperature can induce early bolting, which decreases both the quality and production of lettuce. However, knowledge of underlying lettuce bolting is still lacking. To better understand the molecular basis of bolting, a comparative proteomics analysis was conducted on lettuce stems, during the bolting period induced by a high temperature (33 °C) and a control temperature (20 °C) using iTRAQ-based proteomics, phenotypic measures, and biological verifications using qRT-PCR and Western blot. The high temperature induced lettuce bolting, while the control temperature did not. Of the 5454 identified proteins, 619 proteins presented differential abundance induced by high-temperature relative to the control group, of which 345 had an increased abundance and 274 had a decreased abundance. Proteins with an abundance level change were mainly enriched in pathways associated with photosynthesis and tryptophan metabolism involved in auxin (IAA) biosynthesis. Moreover, among the proteins with differential abundance, proteins associated with photosynthesis and tryptophan metabolism were increased. These findings indicate that a high temperature enhances the function of photosynthesis and IAA biosynthesis to promote the process of bolting, which is in line with the physiology and transcription level of IAA metabolism. Our data provide a first comprehensive dataset for gaining novel understanding of the molecular basis underlying lettuce bolting induced by high temperature. It is potentially important for further functional analysis and genetic manipulation for molecular breeding to breed new cultivars of lettuce to restrain early bolting, which is vital for improving vegetable quality.

scholarly journals Quantitative Proteomic Analyses Reveal That Energy Metabolism and Protein Biosynthesis Reinitiation are Responsible for the Initiation of Bolting Induced by High Temperature in Lettuce (Lactuca sativa L.)

2020 ◽  
Author(s):  
Jing-hong Hao ◽  
He-Nan Su ◽  
Li-li Zhang ◽  
Chao-jie Liu ◽  
Ying-yan Han ◽  
...  
Keyword(s):  
High Temperature ◽  
Energy Metabolism ◽  
Lactuca Sativa ◽  
Ribosomal Proteins ◽  
Protein Biosynthesis ◽  
Temperature Treatment ◽  
High Temperature Treatment ◽  
Lactuca Sativa L ◽  
Considerable Loss ◽  
Initiation Period

Abstract Background Lettuce (Lactuca sativa L.), one of the most economically important leaf vegetables, exhibits early bolting under high-temperature conditions. Early bolting leads to loss of commodity value and edibility, leading to considerable loss and waste of resources. However, the initiation and molecular mechanism underlying early bolting induced by high temperature remains largely elusive. Results To better understand this phenomenon, the bolting initiation period of lettuce was defined, and a comparative proteomic analysis was conducted in the initiation period of bolting induced by a high temperature (33 °C) and a control temperature (20 °C) using iTRAQ-based proteomics, phenotypic measurement, and biological verification by RT-qPCR. Morphological and microscopic observation showed that the initiation of bolting occurred 8 days after high-temperature treatment. Fructose rapidly accumulated after high-temperature treatment. During bolting initiation, of the 3305 identified proteins, a total of 93 proteins exhibited differential abundances, 38 of which were up-regulated, and 55 were down-regulated. Approximately 38% of the proteins were involved in metabolic pathways and were mainly clustered in energy metabolism and protein synthesis. Furthermore, some proteins involved in sugar synthesis were differentially expressed, which were also associated with energy production. Conclusions This is the first report on the metabolic changes involved in bolting initiation in lettuce. Our study suggested that energy metabolism and ribosomal proteins are pivotal components during bolting initiation. This study could provide a potential regulatory mechanism for the initiation of early bolting by high temperature, which could have applications in the manipulation of lettuce for breeding.

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