A Cluster of MYB Transcription Factors Regulates Anthocyanin Biosynthesis in Carrot (Daucus carota L.) Root and Petiole

Flower color is the decisive factor that affects the commercial value of ornamental flowers. Therefore, it is important to study the regulation of flower color formation in lily to discover the positive and negative factors that regulate this important trait. In this study, MYB transcription factors (TFs) were characterized to understand the regulatory mechanism of anthocyanin biosynthesis in lily. Two R2R3-MYB TFs, LvMYB5, and LvMYB1, were found to regulate anthocyanin biosynthesis in lily flowers. LvMYB5, which has an activation motif, belongs to the SG6 MYB protein subgroup of Arabidopsis thaliana. Transient expression of LvMYB5 indicated that LvMYB5 can promote coloration in Nicotiana benthamiana leaves, and that expression of LvMYB5 increases the expression levels of NbCHS, NbDFR, and NbANS. VIGS experiments in lily petals showed that the accumulation of anthocyanins was reduced when LvMYB5 was silenced. Luciferase assays showed that LvMYB5 can promote anthocyanin synthesis by activating the ANS gene promoter. Therefore, LvMYB5 plays an important role in flower coloration in lily. In addition, the transient expression experiment provided preliminary evidence that LvMYB1 (an R2R3-MYB TF) inhibits anthocyanin synthesis in lily flowers. The discovery of activating and inhibitory factors related to anthocyanin biosynthesis in lily provides a theoretical basis for improving flower color through genetic engineering. The results of our study provide a new direction for the further study of the mechanisms of flower color formation in lilies.

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Genome-wide analysis of AP2/ERF transcription factors in carrot (Daucus carota L.) reveals evolution and expression profiles under abiotic stress

Molecular Genetics and Genomics ◽

10.1007/s00438-015-1061-3 ◽

2015 ◽

Vol 290 (6) ◽

pp. 2049-2061 ◽

Cited By ~ 22

Author(s):

Meng-Yao Li ◽

Zhi-Sheng Xu ◽

Ying Huang ◽

Chang Tian ◽

Feng Wang ◽

...

Keyword(s):

Transcription Factors ◽

Abiotic Stress ◽

Daucus Carota ◽

Expression Profiles ◽

Daucus Carota L ◽

Genome Wide Analysis ◽

Genome Wide ◽

Erf Transcription Factors

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Identification of transcription factor genes involved in anthocyanin biosynthesis in carrot (Daucus carota L.) using RNA-Seq

BMC Genomics ◽

10.1186/s12864-018-5135-6 ◽

2018 ◽

Vol 19 (1) ◽

Cited By ~ 12

Author(s):

Miyako Kodama ◽

Henrik Brinch-Pedersen ◽

Shrikant Sharma ◽

Inger Bæksted Holme ◽

Bjarne Joernsgaard ◽

...

Keyword(s):

Transcription Factor ◽

Daucus Carota ◽

Anthocyanin Biosynthesis ◽

Rna Seq ◽

Daucus Carota L ◽

Transcription Factor Genes

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Identification of Two Novel R2R3-MYB Transcription factors, PsMYB114L and PsMYB12L, Related to Anthocyanin Biosynthesis in Paeonia suffruticosa

International Journal of Molecular Sciences ◽

10.3390/ijms20051055 ◽

2019 ◽

Vol 20 (5) ◽

pp. 1055 ◽

Cited By ~ 4

Author(s):

Xinpeng Zhang ◽

Zongda Xu ◽

Xiaoyan Yu ◽

Lanyong Zhao ◽

Mingyuan Zhao ◽

...

Keyword(s):

Transcription Factors ◽

Anthocyanin Biosynthesis ◽

Critical Role ◽

Flower Color ◽

Anthocyanin Accumulation ◽

Paeonia Suffruticosa ◽

Myb Transcription Factors ◽

Transgenic Lines ◽

R2r3 Myb ◽

R2r3 Myb Transcription Factors

Flower color is a charming phenotype with very important ornamental and commercial values. Anthocyanins play a critical role in determining flower color pattern formation, and their biosynthesis is typically regulated by R2R3-MYB transcription factors (TFs). Paeonia suffruticosa is a famous ornamental plant with colorful flowers. However, little is known about the R2R3-MYB TFs that regulate anthocyanin accumulation in P. suffruticosa. In the present study, two R2R3-MYB TFs, namely, PsMYB114L and PsMYB12L, were isolated from the petals of P. suffruticosa ‘Shima Nishiki’ and functionally characterized. Sequence analysis suggested that PsMYB114L contained a bHLH-interaction motif, whereas PsMYB12L contained two flavonol-specific motifs (SG7 and SG7-2). Subsequently, the in vivo function of PsMYB114L and PsMYB12L was investigated by their heterologous expression in Arabidopsis thaliana and apple calli. In transgenic Arabidopsis plants, overexpression of PsMYB114L and of PsMYB12L caused a significantly higher accumulation of anthocyanins, resulting in purple-red leaves. Transgenic apple calli overexpressing PsMYB114L and PsMYB12L also significantly enhanced the anthocyanins content and resulted in a change in the callus color to red. Meanwhile, gene expression analysis in A. thaliana and apple calli suggested that the expression levels of the flavonol synthase (MdFLS) and anthocyanidin reductase (MdANR) genes were significantly downregulated and the dihydroflavonol 4-reductase (AtDFR) and anthocyanin synthase (AtANS) genes were significantly upregulated in transgenic lines of PsMYB114L. Moreover, the expression level of the FLS gene (MdFLS) was significantly downregulated and the DFR (AtDFR/MdDFR) and ANS (AtANS/MdANS) genes were all significantly upregulated in transgenic lines plants of PsMYB12L. These results indicate that PsMYB114L and PsMYB12L both enhance anthocyanin accumulation by specifically regulating the expression of some anthocyanin biosynthesis-related genes in different plant species. Together, these results provide a valuable resource with which to further study the regulatory mechanism of anthocyanin biosynthesis in P. suffruticosa and for the breeding of tree peony cultivars with novel and charming flower colors.

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