Genome-Wide Mining of MYB Transcription Factors in the Anthocyanin Biosynthesis Pathway of Gossypium Hirsutum

2021 ◽  
Author(s):  
Yingjie Zhu ◽  
Ying Bao
Keyword(s):  
Transcription Factors ◽  
Gossypium Hirsutum ◽  
Anthocyanin Biosynthesis ◽  
Biosynthesis Pathway ◽  
Myb Transcription Factors ◽  
Genome Wide

scholarly journals Genome-wide characterization and expression analysis of MYB transcription factors in Gossypium hirsutum

BMC Genetics ◽  
2016 ◽  
Vol 17 (1) ◽  
Author(s):  
Haron Salih ◽  
Wenfang Gong ◽  
Shoupu He ◽  
Gaofei Sun ◽  
Junling Sun ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Gossypium Hirsutum ◽  
Expression Analysis ◽  
Myb Transcription Factors ◽  
Genome Wide

scholarly journals Construction of yeast one-hybrid library and screening of transcription factors regulating LhMYBSPLATTER expression in Asiatic hybrid lilies (Lilium spp.)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yuwei Cao ◽  
Mengmeng Bi ◽  
Panpan Yang ◽  
Meng Song ◽  
Guoren He ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Theoretical Basis ◽  
Anthocyanin Biosynthesis ◽  
Regulatory Proteins ◽  
Biosynthesis Pathway ◽  
Temporal Expression ◽  
Anthocyanin Pigmentation ◽  
Cdna Insert ◽  
R2r3 Myb ◽  
First Time

Abstract Background Anthocyanins, which belong to flavonoids, are widely colored among red-purple pigments in the Asiatic hybrid lilies (Lilium spp.). Transcription factor (TF) LhMYBSPLATTER (formerly known as LhMYB12-Lat), identified as the major kernel protein, regulating the anthocyanin biosynthesis pathway in ‘Tiny Padhye’ of Tango Series cultivars, which the pigmentation density is high in the lower half of tepals and this patterning is of exceptional ornamental value. However, the research on mechanism of regulating the spatial and temporal expression differences of LhMYBSPLATTER, which belongs to the R2R3-MYB subfamily, is still not well established. To explore the molecular mechanism of directly related regulatory proteins of LhMYBSPLATTER in the anthocyanin pigmentation, the yeast one-hybrid (Y1H) cDNA library was constructed and characterized. Results In this study, we describe a yeast one-hybrid library to screen transcription factors that regulate LhMYBSPLATTER gene expression in Lilium, with the library recombinant efficiency of over 98%. The lengths of inserted fragments ranged from 400 to 2000 bp, and the library capacity reached 1.6 × 106 CFU of cDNA insert, which is suitable to fulfill subsequent screening. Finally, seven prey proteins, including BTF3, MYB4, IAA6-like, ERF4, ARR1, ERF WIN1-like, and ERF061 were screened by the recombinant bait plasmid and verified by interaction with the LhMYBSPLATTER promoter. Among them, ERFs, AUX/IAA, and BTF3 may participate in the negative regulation of the anthocyanin biosynthesis pathway in Lilium. Conclusion A yeast one-hybrid library of lily was successfully constructed in the tepals for the first time. Seven candidate TFs of LhMYBSPLATTER were screened, which may provide a theoretical basis for the study of floral pigmentation.

scholarly journals Genome-wide analysis and expression profiles of the StR2R3-MYB transcription factor superfamily in Solanum Tuberosum

2019 ◽  
Author(s):  
Yuanming Li ◽  
Kui Lin-Wang ◽  
Zhen Liu ◽  
Andrew C. Allan ◽  
Junlian Zhang ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Abiotic Stress ◽  
Solanum Tuberosum ◽  
Drought Stress ◽  
Anthocyanin Biosynthesis ◽  
Rna Seq ◽  
Genome Wide ◽  
Ear Motif ◽  
Myb Genes ◽  
Myb Proteins

Abstract Background: MYB transcription factors comprise one of the largest families in plant kingdom, which play a variety of functions in plant developmental processes and defence responses. However, it has not been systematically studied in Potato (Solanum tuberosum), which is the most important non-cereal crop world-wide. Results: In the present study, a total of 108 StR2R3-MYB transcription factors were identified and further phylogenetically classified into 28 subfamilies, as supported by highly conserved gene structures and motifs. Collinearity analysis showed that the segmental duplication events played a crucial role in the expansion of StR2R3-MYB gene family. Synteny analysis indicated that 37 and 13 StR2R3-MYB genes were orthologous to Arabidopsis and wheat, respectively, and these gene pairs have evolved under strong purifying selection. RNA-seq data from different tissues and abiotic stresses revealed tissue-preferential and abiotic stress-responsive StR2R3-MYB genes. We further analyzed StR2R3-MYB genes might be involved in anthocyanin biosynthesis and drought stress by using RNA-seq data of pigmented tetraploid potato cultivars and drought-sensitive and -tolerant tetraploid potato cultivars under drought stress, respectively. Moreover, EAR motifs were found in 21 StR2R3-MYB proteins and 446 pairs of proteins were predicted to interact with 21 EAR motif-containing StR2R3-MYB proteins by constructing the interaction network with medium confidence (0.4). Additionally, Gene Ontology (GO) analysis of the 21 EAR motif-containing StR2R3-MYB proteins was performed to further investigate their functions. Conclusions: In this work, we systematically identified StR2R3-MYB genes by analyzing the potato genome sequence using a set of bioinformatics approaches. Genome-wide comparative analysis of StR2R3-MYB genes and their expression analysis identified members of this superfamily may be involved in tissue-specific development, anthocyanin biosynthesis and abiotic stress responses.

scholarly journals Regulation of MYB Transcription Factors of Anthocyanin Synthesis in Lily Flowers

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiaojuan Yin ◽  
Yibing Zhang ◽  
Li Zhang ◽  
Baohua Wang ◽  
Yidi Zhao ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Transient Expression ◽  
Anthocyanin Biosynthesis ◽  
Gene Promoter ◽  
Flower Color ◽  
Preliminary Evidence ◽  
Anthocyanin Synthesis ◽  
Myb Transcription Factors ◽  
Color Formation ◽  
R2r3 Myb

Flower color is the decisive factor that affects the commercial value of ornamental flowers. Therefore, it is important to study the regulation of flower color formation in lily to discover the positive and negative factors that regulate this important trait. In this study, MYB transcription factors (TFs) were characterized to understand the regulatory mechanism of anthocyanin biosynthesis in lily. Two R2R3-MYB TFs, LvMYB5, and LvMYB1, were found to regulate anthocyanin biosynthesis in lily flowers. LvMYB5, which has an activation motif, belongs to the SG6 MYB protein subgroup of Arabidopsis thaliana. Transient expression of LvMYB5 indicated that LvMYB5 can promote coloration in Nicotiana benthamiana leaves, and that expression of LvMYB5 increases the expression levels of NbCHS, NbDFR, and NbANS. VIGS experiments in lily petals showed that the accumulation of anthocyanins was reduced when LvMYB5 was silenced. Luciferase assays showed that LvMYB5 can promote anthocyanin synthesis by activating the ANS gene promoter. Therefore, LvMYB5 plays an important role in flower coloration in lily. In addition, the transient expression experiment provided preliminary evidence that LvMYB1 (an R2R3-MYB TF) inhibits anthocyanin synthesis in lily flowers. The discovery of activating and inhibitory factors related to anthocyanin biosynthesis in lily provides a theoretical basis for improving flower color through genetic engineering. The results of our study provide a new direction for the further study of the mechanisms of flower color formation in lilies.

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