Systematic Analysis and Functional Validation of Citrus XTH Genes Reveal the Role of Csxth04 in Citrus Bacterial Canker Resistance and Tolerance

The present study was designed to serve as a comprehensive analysis of Citrus sinensis (C. sinensis) pectin acetylesterases (CsPAEs), and to assess the roles of these PAEs involved in the development of citrus bacterial canker (CBC) caused by Xanthomonas citri subsp. citri (Xcc) infection. A total of six CsPAEs were identified in the genome of C. sinensis, with these genes being unevenly distributed across chromosomes 3, 6, and 9, and the unassembled scaffolds. A subset of CsPAEs were found to be involved in responses to Xcc infection. In particular, CsPAE2 was identified to be associated with such infections, as it was upregulated in CBC-susceptible variety Wanjincheng and inversely in CBC-resistant variety Calamondin. Transgenic citrus plants overexpressing CsPAE2 were found to be more susceptible to CBC, whereas the silencing of this gene was sufficient to confer CBC resistance. Together, these findings provide evolutionary insights into and functional information about the CsPAE family. This study also suggests that CsPAE2 is a potential candidate gene that negatively contributes to bacterial canker disease and can be used to breed CBC-resistant citrus plants.

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CsPrx25, a class III peroxidase in Citrus sinensis, confers resistance to citrus bacterial canker through the maintenance of ROS homeostasis and cell wall lignification

Horticulture Research ◽

10.1038/s41438-020-00415-9 ◽

2020 ◽

Vol 7 (1) ◽

Cited By ~ 1

Author(s):

Qiang Li ◽

Xiujuan Qin ◽

Jingjing Qi ◽

Wanfu Dou ◽

Christophe Dunand ◽

...

Keyword(s):

Cell Wall ◽

Susceptible Variety ◽

Resistant Variety ◽

Bacterial Canker ◽

Class Iii ◽

Ros Homeostasis ◽

Citrus Production ◽

Citrus Bacterial Canker ◽

Apoplastic Barrier

AbstractCitrus bacterial canker (CBC) results from Xanthomonas citri subsp. citri (Xcc) infection and poses a grave threat to citrus production. Class III peroxidases (CIII Prxs) are key proteins to the environmental adaptation of citrus plants to a range of exogenous pathogens, but the role of CIII Prxs during plant resistance to CBC is poorly defined. Herein, we explored the role of CsPrx25 and its contribution to plant defenses in molecular detail. Based on the expression analysis, CsPrx25 was identified as an apoplast-localized protein that is differentially regulated by Xcc infection, salicylic acid, and methyl jasmone acid in the CBC-susceptible variety Wanjincheng (C. sinensis) and the CBC-resistant variety Calamondin (C. madurensis). Transgenic Wanjincheng plants overexpressing CsPrx25 were generated, and these transgenic plants exhibited significantly increased CBC resistance compared with the WT plants. In addition, the CsPrx25-overexpressing plants displayed altered reactive oxygen species (ROS) homeostasis accompanied by enhanced H2O2 levels, which led to stronger hypersensitivity responses during Xcc infection. Moreover, the overexpression of CsPrx25 enhanced lignification as an apoplastic barrier for Xcc infection. Taken together, the results highlight how CsPrx25-mediated ROS homeostasis reconstruction and cell wall lignification can enhance the resistance of sweet orange to CBC.

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The Role of Evaluative Tools in the Creation of the Memorial Media Portrait of Jacques Chirac (1932—2019)

Nauchnyy dialog ◽

10.24224/2227-1295-2020-9-97-110 ◽

2020 ◽

pp. 97-110

Author(s):

E. N. Mikhailova ◽

V. A. Telegina

Keyword(s):

Print Media ◽

Political Elite ◽

Systematic Analysis ◽

Research Material ◽

Political Orientations ◽

Different Types ◽

The Media ◽

The Creation ◽

Entire Text

The article is devoted to the study of evaluative tools used in modern French media in order to form the media image of a representative of the political elite. The techniques used in the creation of a memorial media portrait of Jacques Chirac (1932—2019), President of France from 1995 to 2007 are considered. The research material was the most prestigious French print media of various political orientations, published in late September — early October 2019 in connection with the death of the ex-President of the French Republic. The relevance of the research topic is dictated by the close attention of modern linguistics to axiological phenomena, differently presented in different types of discursive practices. The novelty of the study is due to the appeal to the analysis of the complex of evaluation tools used in the French print media when characterizing the former leader of the state during the nation’s farewell period. The estimated potential of the title of the article and its influence on the formation of the estimated vector of the entire text of the publication are shown. A systematic analysis of the assessment expression means, reflected in the memorial media portrait of the politician, is given. The factors that influenced the peculiarities of their use in this type of media portrait are revealed.

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Neoliberalism with Scandinavian characteristics: The slow formation of neoliberal common sense in Denmark

Capital & Class ◽

10.1177/03098168211029001 ◽

2021 ◽

pp. 030981682110290

Author(s):

Rune Møller Stahl

Keyword(s):

International Economic ◽

Common Sense ◽

Causal Power ◽

Policy Instrument ◽

Alternative Analysis ◽

Systematic Analysis ◽

Economic Council ◽

Ideational Change ◽

Economic Developments

This article describes the ascension of neoliberal economic ideas in the macroeconomic establishment in Denmark. Based on a systematic analysis of documents from the Danish government and the Economic Council from the 1970s to the early 2000s, the article traces the development of the economic ideas and policy instrument that dominate the analytical process of the Danish macroeconomic establishment. The article applies a Gramscian-inspired framework to track the gradual and uneven process under which neoliberal economic ideas became common sense in the Danish context. This framework challenges some of the assumptions of the ideational focus of much constructivist literature, and offers an alternative analysis focused on the legitimating role of economic ideas. As much of the ideational change took place after policy adaptions to international economic developments, the Danish case provides little support for the theory of the causal power of ideas. Rather, it seems as though economic models and ideas are imported as ‘after the fact’ legitimations of changes in policy.

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Enzymatic activities of citrus leaves from plants re resistant and susceptible to citrus bacterial canker disease

Environmental and Experimental Botany ◽

10.1016/0098-8472(92)90059-b ◽

1992 ◽

Vol 32 (4) ◽

pp. 465-470 ◽

Cited By ~ 7

Author(s):

H.J. Jiao ◽

S.Y. Wang ◽

E.L. Civerolo

Keyword(s):

Enzymatic Activities ◽

Bacterial Canker ◽

Canker Disease ◽

Citrus Bacterial Canker ◽

Citrus Leaves

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Cell Therapy in Patients with Critical Limb Ischemia

Stem Cells International ◽

10.1155/2015/931420 ◽

2015 ◽

Vol 2015 ◽

pp. 1-13 ◽

Cited By ~ 27

Author(s):

Rita Compagna ◽

Bruno Amato ◽

Salvatore Massa ◽

Maurizio Amato ◽

Raffaele Grande ◽

...

Keyword(s):

Cell Therapy ◽

Critical Limb Ischemia ◽

Limb Ischemia ◽

Tissue Loss ◽

Systematic Analysis ◽

Peripheral Arterial Obstructive Disease ◽

Peripheral Arterial ◽

Cellular Components

Critical limb ischemia (CLI) represents the most advanced stage of peripheral arterial obstructive disease (PAOD) with a severe obstruction of the arteries which markedly reduces blood flow to the extremities and has progressed to the point of severe rest pain and/or even tissue loss. Recent therapeutic strategies have focused on restoring this balance in favor of tissue survival using exogenous molecular and cellular agents to promote regeneration of the vasculature. These are based on stimulation of angiogenesis by extracellular and cellular components. This review article carries out a systematic analysis of the most recent scientific literature on the application of stem cells in patients with CLI. The results obtained from the detailed analysis of the recent literature data have confirmed the beneficial role of cell therapy in reducing the rate of major amputations in patients with CLI and improving their quality of life.

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Computational modelling of mitotic exit in budding yeast: the role of separase and Cdc14 endocycles

Journal of The Royal Society Interface ◽

10.1098/rsif.2010.0649 ◽

2011 ◽

Vol 8 (61) ◽

pp. 1128-1141 ◽

Cited By ~ 20

Author(s):

P. K. Vinod ◽

Paula Freire ◽

Ahmed Rattani ◽

Andrea Ciliberto ◽

Frank Uhlmann ◽

...

Keyword(s):

Regulatory Networks ◽

Budding Yeast ◽

Cell Cycle Control ◽

Computational Modelling ◽

Eukaryotic Cell ◽

Mitotic Exit ◽

Intuitive Reasoning ◽

Systematic Analysis ◽

Systems View

The operating principles of complex regulatory networks are best understood with the help of mathematical modelling rather than by intuitive reasoning. Hereby, we study the dynamics of the mitotic exit (ME) control system in budding yeast by further developing the Queralt's model. A comprehensive systems view of the network regulating ME is provided based on classical experiments in the literature. In this picture, Cdc20–APC is a critical node controlling both cyclin (Clb2 and Clb5) and phosphatase (Cdc14) branches of the regulatory network. On the basis of experimental situations ranging from single to quintuple mutants, the kinetic parameters of the network are estimated. Numerical analysis of the model quantifies the dependence of ME control on the proteolytic and non-proteolytic functions of separase. We show that the requirement of the non-proteolytic function of separase for ME depends on cyclin-dependent kinase activity. The model is also used for the systematic analysis of the recently discovered Cdc14 endocycles. The significance of Cdc14 endocycles in eukaryotic cell cycle control is discussed as well.

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Vertical Delivery as a Method of Prevention of Obstetric and Perinatal Complications

Acta biomedica scientifica ◽

10.29413/abs.2019-4.5.1 ◽

2019 ◽

Vol 4 (5) ◽

pp. 7-13

Author(s):

M. N. Mochalova ◽

S. V. Novokshanovа ◽

V. A. Mudrov

Keyword(s):

Upright Position ◽

Alternative Methods ◽

Vertical Position ◽

Perinatal Complications ◽

Systematic Analysis ◽

Birth Canal ◽

Early Postpartum ◽

Analysis And Synthesis ◽

Selection Of

Currently, there are many points of view on management of physiological labor, in particular, it concerns the intrapartum position of a woman. Tactics of modern obstetrics should ensure the safety of motherhood, which in future ensures the prosperity of the state. One of the alternative methods of delivery is the vertical position of a woman in the intrapartum period. It is impossible to describe the whole range of possible positions of a woman in the intrapartum period, the common ones being: lying position (lateral, reclining, lithotomy, Trendelenburg’s, etc.) or upright position (sitting, using a chair for childbirth, standing, squatting, standing on the knees, etc.). Opinions about how the vertical position of a mother in the intrapartum period affects the outcome of childbirth are quite ambiguous. The conclusions of various authors on that matter often contradict each other.The aim of the research was to study the role of vertical delivery in reducing the frequency of obstetric and perinatal complications.Materials: publications of foreign and domestic authors within the period from 1989 to 2017.Methods: systematic analysis and synthesis of literature data.Conclusion: Despite a significant number of studies, it is not possible to determine the universality of the vertical position in childbirth, therefore, the selection of patients for the management of vertical childbirth should be approached carefully. In the presence of pregnancy complications, preference should be given to the classic version of the position in childbirth. In women who have a low risk of perinatal complications, an upright position can be a worthy alternative. To prevent the development of bleeding in the III stage of labor and the early postpartum period, a woman should take a horizontal position after the birth of the fetus. This condition must also be observed when examining the birth canal. Thus, the rational tactics of conducting childbirth is to determine the optimal combination of vertical and horizontal positions at different periods of the childbirth process, taking into account the convenience for the woman in labor.

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Migration Discourse in Sweden: Frames and Sentiments in Mainstream and Social Media

10.31235/osf.io/m5cjh ◽

2020 ◽

Author(s):

Victoria Yantseva

Keyword(s):

Social Media ◽

Historical Context ◽

Media Discourse ◽

Refugee Crisis ◽

Long Term Effects ◽

Data Set ◽

Systematic Analysis ◽

The Media ◽

European Refugee Crisis

This study undertakes a systematic analysis of media discourse on migration in Sweden from 2012 to 2019. Using a novel data set consisting of mainstream newspapers, Twitter and forum data, the study answers two questions: What do Swedish media actually talk about when they talk about “migration”? And how do they talk about it? Using a combination of computational text analysis tools, I analyze a shift in the media discourse seen as one of the outcomes of the European refugee crisis in 2015 and try to understand the role of social media in this process. The results of the study indicate that messages on social media generally had negative tonality and suggest that some of the media frames can be attributed to a migration-hostile discourse. At the same time, the analysis of framing and sentiment dynamics provides little evidence for the discourse shift and any long-term effects of the European refugee crisis on the Swedish media discourse. Rather, one can hypothesize that the role of the crisis should be viewed in a broader political and historical context.

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