scholarly journals The Halophyte Halostachys caspica AP2/ERF Transcription Factor HcTOE3 Positively Regulates Freezing Tolerance in Arabidopsis

2021 ◽  
Vol 12 ◽  
Author(s):  
Fangliu Yin ◽  
Youling Zeng ◽  
Jieyun Ji ◽  
Pengju Wang ◽  
Yufang Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abscisic Acid ◽  
Freezing Tolerance ◽  
Critical Role ◽  
Extreme Temperature ◽  
Freezing Stress ◽  
Antioxidant Enzyme Activities ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Halostachys Caspica

The APETALA2 (AP2) and ethylene-responsive element-binding factor (ERF) gene family is one of the largest plant-specific transcription factor gene families, which plays a critical role in plant development and evolution, as well as response to various stresses. The TARGET OF EAT3 (TOE3) gene is derived from Halostachys caspica and belongs to the AP2 subfamily with two AP2 DNA-binding domains. Currently, AP2 family mainly plays crucial roles in plant growth and evolution, yet there are few reports about the role of AP2 in abiotic stress tolerance. Here, we report HcTOE3, a new cold-regulated transcription factor gene, which has an important contribution to freezing tolerance. The main results showed that the expression of HcTOE3 in the H. caspica assimilating branches was strongly induced by different abiotic stresses, including high salinity, drought, and extreme temperature (heat, chilling, and freezing), as well as abscisic acid and methyl viologen treatments. Overexpressing HcTOE3 gene (OE) induced transgenic Arabidopsis plant tolerance to freezing stress. Under freezing treatment, the OE lines showed lower content of malondialdehyde and electrolyte leakage and less accumulation of reactive oxygen species compared with the wild type. However, the survival rates, antioxidant enzyme activities, and contents of osmotic adjustment substance proline were enhanced in transgenic plants. Additionally, the OE lines increased freezing tolerance by up-regulating the transcription level of cold responsive genes (CBF1, CBF2, COR15, COR47, KIN1, and RD29A) and abscisic acid signal transduction pathway genes (ABI1, ABI2, ABI5, and RAB18). Our results suggested that HcTOE3 positively regulated freezing stress and has a great potential as a candidate gene to improve plant freezing tolerance.

ERF105is a transcription factor gene ofArabidopsis thalianarequired for freezing tolerance and cold acclimation

2016 ◽  
Vol 40 (1) ◽  
pp. 108-120 ◽  
Author(s):  
Sylvia Bolt ◽  
Ellen Zuther ◽  
Stefanie Zintl ◽  
Dirk K. Hincha ◽  
Thomas Schmülling
Keyword(s):  
Transcription Factor ◽  
Cold Acclimation ◽  
Freezing Tolerance ◽  
Transcription Factor Gene ◽  
Factor Gene

scholarly journals Inactivation of Transcription Factor Gene ACE2 in the Fungal Pathogen Candida glabrata Results in Hypervirulence

2004 ◽  
Vol 3 (2) ◽  
pp. 546-552 ◽  
Author(s):  
Mohammed Kamran ◽  
Ana-Maria Calcagno ◽  
Helen Findon ◽  
Elaine Bignell ◽  
Michael D. Jones ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Candida Glabrata ◽  
Critical Role ◽  
Innate Immune ◽  
Clear Understanding ◽  
Wild Type ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Systemic Infections

ABSTRACT During an infection, the coordinated orchestration of many factors by the invading organism is required for disease to be initiated and to progress. The elucidation of the processes involved is critical to the development of a clear understanding of host-pathogen interactions. For Candida species, the inactivation of many fungal attributes has been shown to result in attenuation. Here we demonstrate that the Candida glabrata homolog of the Saccharomyces cerevisiae transcription factor gene ACE2 encodes a function that mediates virulence in a novel way. Inactivation of C. glabrata ACE2 does not result in attenuation but, conversely, in a strain that is hypervirulent in a murine model of invasive candidiasis. C. glabrata ace2 null mutants cause systemic infections characterized by fungal escape from the vasculature, tissue penetration, proliferation in vivo, and considerable overstimulation of the proinflammatory arm of the innate immune response. Compared to the case with wild-type fungi, mortality occurs much earlier in mice infected with C. glabrata ace2 cells, and furthermore, 200-fold lower doses are required to induce uniformly fatal infections. These data demonstrate that C. glabrata ACE2 encodes a function that plays a critical role in mediating the host-Candida interaction. It is the first virulence-moderating gene to be described for a Candida species.

Sign in / Sign up

Export Citation Format

Share Document