Influence of Switchgrass TDIF-like Genes on Arabidopsis Vascular Development

As a member of the CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION (CLE) family, the dodecapeptide tracheary element differentiation inhibitory factor (TDIF) has a major impact on vascular development in plants. However, the influence of polymorphisms in the TDIF peptide motif on activity remains poorly understood. The model plant, Arabidopsis provides a fast and effective tool for assaying the activity of TDIF homologs. Five TDIF homologs from a group of 93 CLE genes in switchgrass (Panicum virgatum), a perennial biomass crop, named PvTDIF-like (PvTDIFL) genes were studied. The expression levels of PvTDIFL1, PvTDIFL3MR3, and PvTDIFL3MR2 were relatively high and all of them were expressed at the highest levels in the rachis of switchgrass. The precursor proteins for PvTDIFL1, PvTDIFL3MR3, and PvTDIFL3MR2 contained one, three, and two TDIFL motifs, respectively. Treatments with exogenous PvTDIFL peptides increased the number of stele cells in the hypocotyls of Arabidopsis seedlings, with the exception of PvTDIFL_4p. Heterologous expression of PvTDIFL1 in Arabidopsis strongly inhibited plant growth, increased cell division in the vascular tissue of the hypocotyl, and disrupted the cellular organization of the hypocotyl. Although heterologous expression of PvTDIFL3MR3 and PvTDIFL3MR2 also affected plant growth and vascular development, PvTDIFL activity was not enhanced by the multiple TDIFL motifs encoded by PvTDIFL3MR3 and PvTDIFL3MR2. These data indicate that in general, PvTDIFLs are functionally similar to Arabidopsis TDIF but that the processing and activities of the PvTDIFL peptides are more complex.

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A survey of endophytic fungi of switchgrass (Panicum virgatum) in the Midwest, and their putative roles in plant growth

Fungal Ecology ◽

10.1016/j.funeco.2011.12.006 ◽

2012 ◽

Vol 5 (5) ◽

pp. 521-529 ◽

Cited By ~ 35

Author(s):

N.M. Kleczewski ◽

J.T. Bauer ◽

J.D. Bever ◽

K. Clay ◽

H.L. Reynolds

Keyword(s):

Plant Growth ◽

Endophytic Fungi ◽

Panicum Virgatum

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Changes in the Synthesis of RNA and Protein during Tracheary Element Differentiation in Single Cells Isolated from the Mesophyll of Zinnia elegans

Plant and Cell Physiology ◽

10.1093/oxfordjournals.pcp.a076556 ◽

1983 ◽

Vol 24 (4) ◽

pp. 603-614 ◽

Cited By ~ 19

Author(s):

Hiroo Fukuda ◽

Atsushi Komamine

Keyword(s):

Single Cells ◽

Tracheary Element ◽

Zinnia Elegans ◽

Tracheary Element Differentiation

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Relationship between Tracheary Element Differentiation and DNA Synthesis in Single Cells Isolated from the Mesophyll of Zinnia elegans—Analysis by Inhibitors of DNA Synthesis

Plant and Cell Physiology ◽

10.1093/oxfordjournals.pcp.a076144 ◽

1981 ◽

Keyword(s):

Dna Synthesis ◽

Single Cells ◽

Tracheary Element ◽

Zinnia Elegans ◽

Tracheary Element Differentiation

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Final and Fatal Step of Tracheary Element Differentiation

Progress in Biotechnology - Molecular Breeding of Woody Plants, Proceedings of the International Wood Biotechnology Symposium (IWBS) ◽

10.1016/s0921-0423(01)80053-8 ◽

2001 ◽

pp. 29-42 ◽

Cited By ~ 1

Author(s):

Alan M. Jones ◽

Andrew Groover ◽

Xiaohong Yu ◽

Tony Perdue

Keyword(s):

Tracheary Element ◽

Tracheary Element Differentiation

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Genome-Wide Analysis of the TCP Gene Family in Switchgrass (Panicum virgatum L.)

International Journal of Genomics ◽

10.1155/2019/8514928 ◽

2019 ◽

Vol 2019 ◽

pp. 1-13 ◽

Cited By ~ 2

Author(s):

Yuzhu Huo ◽

Wangdan Xiong ◽

Kunlong Su ◽

Yu Li ◽

Yawen Yang ◽

...

Keyword(s):

Salt Stress ◽

Plant Growth ◽

Gene Family ◽

Stress Responses ◽

Panicum Virgatum ◽

Expression Profiles ◽

Specific Expression ◽

Genome Wide Analysis ◽

Genome Wide ◽

A Genome

The plant-specific transcription factor TCPs play multiple roles in plant growth, development, and stress responses. However, a genome-wide analysis of TCP proteins and their roles in salt stress has not been declared in switchgrass (Panicum virgatum L.). In this study, 42 PvTCP genes (PvTCPs) were identified from the switchgrass genome and 38 members can be anchored to its chromosomes unevenly. Nine PvTCPs were predicted to be microRNA319 (miR319) targets. Furthermore, PvTCPs can be divided into three clades according to the phylogeny and conserved domains. Members in the same clade have the similar gene structure and motif localization. Although all PvTCPs were expressed in tested tissues, their expression profiles were different under normal condition. The specific expression may indicate their different roles in plant growth and development. In addition, approximately 20 cis-acting elements were detected in the promoters of PvTCPs, and 40% were related to stress response. Moreover, the expression profiles of PvTCPs under salt stress were also analyzed and 29 PvTCPs were regulated after NaCl treatment. Taken together, the PvTCP gene family was analyzed at a genome-wide level and their possible functions in salt stress, which lay the basis for further functional analysis of PvTCPs in switchgrass.

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Nitrogen and phosphorus levels affected plant growth, essential oil composition and antioxidant status of lavender plant (Lavandula angustifolia Mill.)

Industrial Crops and Products ◽

10.1016/j.indcrop.2015.12.067 ◽

2016 ◽

Vol 83 ◽

pp. 577-586 ◽

Cited By ~ 71

Author(s):

Antonios Chrysargyris ◽

Christakis Panayiotou ◽

Nikos Tzortzakis

Keyword(s):

Essential Oil ◽

Plant Growth ◽

Antioxidant Status ◽

Essential Oil Composition ◽

Oil Composition ◽

Nitrogen And Phosphorus ◽

Lavandula Angustifolia ◽

Affected Plant ◽

Phosphorus Levels

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Growth and Nodulation of Provenances of Casuarina cunninghamiana Inoculated With a Range of Frankia Sources

Australian Journal of Botany ◽

10.1071/bt9880171 ◽

1988 ◽

Vol 36 (2) ◽

pp. 171 ◽

Cited By ~ 15

Author(s):

AI Fleming ◽

ER Williams ◽

JW Turnbull

Keyword(s):

Plant Growth ◽

Root Nodules ◽

A Priori ◽

Geographical Location ◽

Seed Sources ◽

Affected Plant ◽

Casuarina Cunninghamiana ◽

Geographical Regions ◽

Major River ◽

Shoot Weight

Seed and root nodules (for use as a Frankia inoculum) collected from 18 provenances of Casuarina cunninghamiana Miq. were used in a complete cross-inoculation experiment conducted in a glasshouse. The provenances covered the geographical range of the species and represented major river systems. They were arranged a priori into five groups according to geographical location. Seventeen of the inocula were effective on seed from all provenances; one inoculum failed to nodulate seedlings from any provenance. Inoculum source, seed source and their interaction all affected plant growth. Greatest shoot weight was obtained with seed and inoculum combinations from similar geographical regions, particularly northern inocula with northern seed sources and southern inocula with southern seed sources. When averaged over all seed sources northern inocula were the most generally effective in promoting plant growth. In contrast, when averaged over all inoculum sources, southern seed sources grew best. These results demonstrate the potential for improving the effectiveness of the C. cunninghamiana-Frankia association in forestry by selection of the symbiotic partners.

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