scholarly journals Molecular Phylogeny and Taxonomy of the Genus Spumella (Chrysophyceae) Based on Morphological and Molecular Evidence

2021 ◽  
Vol 12 ◽  
Author(s):  
Minseok Jeong ◽  
Jong Im Kim ◽  
Seung Won Nam ◽  
Woongghi Shin
Keyword(s):  
New Species ◽  
Species Diversity ◽  
Molecular Phylogeny ◽  
Cyst Formation ◽  
Morphological Data ◽  
Stalk Cell ◽  
Phylogenetic Study ◽  
Lsu Rdna ◽  
Molecular Evidence ◽  
Species Classification

The genus Spumella, established by Cienkowsky in 1870, is characterized by omnivory, two (rarely three) flagella, a short stick-like structure beneath the flagella, a threadlike stalk, cell division via constriction and cyst formation. Since the first phylogenetic study of Spumella-like flagellates, their paraphyly has consistently been shown, with separation into several genera. More recently, Spumella was carefully investigated using molecular and morphological data to propose seven new species. Classification of this genus and knowledge of its species diversity remain limited because Spumella-like flagellates are extremely difficult to identify based on limited morphological characters. To understand the phylogeny and taxonomy of Spumella, we analyzed molecular and morphological data from 47 strains, including 18 strains isolated from Korean ponds or swamps. Nuclear SSU, ITS and LSU rDNA data were used for maximum likelihood and Bayesian analyses. The molecular data divided the strains into 15 clades, including seven new lineages, each with unique molecular signatures for nuclear SSU rRNA from the E23-2 to E23-5 domains, the spacer between the E23-8 and E23-9 domains of the V4 region and domain 29 of the V5 region. Our results revealed increased species diversity in Spumella. In contrast to the molecular phylogeny results, the taxa showed very similar cell morphologies, suggesting morphological convergence into simple nanoflagellates to enable heterotrophy. Three new species produced stomatocysts in culture. Aspects of stomatocyst morphology, including collar structure, surface ornamentation, and cyst shape, were very useful in differentiating the three species. The general ultrastructure of Spumella bureschii strain Baekdongje012018B8 and S. benthica strain Hwarim032418A5 showed the typical chrysophyte form for the leucoplast, a vestigial chloroplast surrounded by four envelope membranes, supporting the hypothesis that Spumella evolved from a phototroph to a heterotroph via the loss of its photosynthetic ability. Seven new species are proposed: S. benthica, S. communis, S. longicolla, S. oblata, S. rotundata, S. similis, and S. sinechrysos.

scholarly journals Corrigendum: He XL, Horak E, Wang D, Li TH, Peng WH, Gan BC (2019) Descriptions of five new species in Entoloma subgenus Claudopus from China, with molecular phylogeny of Entoloma s.l. MycoKeys 61: 1–26. https://doi.org/10.3897/mycokeys.61.46446

MycoKeys ◽  
2020 ◽  
Vol 63 ◽  
pp. 163-172
Author(s):  
Xiao-Lan He ◽  
Egon Horak ◽  
Di Wang ◽  
Tai-Hui Li ◽  
Wei-Hong Peng ◽  
...  
Keyword(s):  
New Species ◽  
Species Diversity ◽  
Molecular Phylogeny ◽  
Novel Species ◽  
Molecular Data ◽  
Monophyletic Group ◽  
Molecular Evidence ◽  
Morphological And Molecular Data ◽  
High Level ◽  
Ecological Range

Entoloma subgenus Claudopus is widely distributed, yet the taxonomy and systematics of its species are still poorly documented. In the present study, more than forty collections of Claudopus were gathered in China and subsequently analysed, based on morphological and molecular data. The results revealed first a high level of species diversity of Claudopus in China and second, there is a wide ecological range regarding the substrates and the habitats ranging from temperate, tropical to subalpine locations. Based on morphological and molecular evidence, five novel species from China are proposed, viz. E. conchatum, E. flabellatum, E. gregarium, E. pleurotoides and E. reductum. Molecular phylogeny of Entoloma s.l. was also reconstructed, based on 187 representatives of Entoloma s.l. by employing the combined ITS, LSU, mtSSU and RPB2 sequences. Ten monophyletic clades (Claudopus, Leptonia, Nolanea, Cuboid-spored Inocephalus, “Alboleptonia”, Cyanula, Pouzarella, Rhodopolia, Prunuloides and Rusticoides) were recovered, while 13 taxa could not be placed in any defined clades. The results confirmed that Claudopus in a traditional morphological sense is not monophyletic and the Rusticoides-group, previously considered within Claudopus, formed a separate clade; but section Claudopus and relatives of E. undatum belong to a distinctive monophyletic group. Despite some monophyletic groups in Entoloma s.l. being distinctive in both morphology and molecular phylogeny, they were still treated as subgenera of Entoloma s.l. temporarily, because accepting them as genera will make Entoloma s.l. paraphyletic.

scholarly journals Descriptions of five new species in Entoloma subgenus Claudopus from China, with molecular phylogeny of Entoloma s.l.

MycoKeys ◽  
2019 ◽  
Vol 61 ◽  
pp. 1-26
Author(s):  
Xiao-Lan He ◽  
Egon Horak ◽  
Di Wang ◽  
Tai-Hui Li ◽  
Wei-Hong Peng ◽  
...  
Keyword(s):  
New Species ◽  
Species Diversity ◽  
Molecular Phylogeny ◽  
Novel Species ◽  
Molecular Data ◽  
Monophyletic Group ◽  
Molecular Evidence ◽  
Morphological And Molecular Data ◽  
High Level ◽  
Ecological Range

Entoloma subgenus Claudopus is widely distributed, yet the taxonomy and systematics of its species are still poorly documented. In the present study, more than forty collections of Claudopus were gathered in China and subsequently analysed, based on morphological and molecular data. The results revealed first a high level of species diversity of Claudopus in China and second, there is a wide ecological range regarding the substrates and the habitats ranging from temperate, tropical to subalpine locations. Based on morphological and molecular evidence, five novel species from China are proposed, viz. E. conchatum, E. flabellatum, E. gregarium, E. pleurotoides and E. reductum. Molecular phylogeny of Entoloma s.l. was also reconstructed, based on 187 representatives of Entoloma s.l. by employing the combined ITS, LSU, mtSSU and RPB2 sequences. Ten monophyletic clades (Claudopus, Leptonia, Nolanea, Cuboid-spored Inocephalus, “Alboleptonia”, Cyanula, Pouzarella, Rhodopolia, Prunuloides and Rusticoides) were recovered, while 13 taxa could not be placed in any defined clades. The results confirmed that Claudopus in a traditional morphological sense is not monophyletic and the Rusticoides-group, previously considered within Claudopus, formed a separate clade; but section Claudopus and relatives of E. undatum belong to a distinctive monophyletic group. Despite some monophyletic groups in Entoloma s.l. being distinctive in both morphology and molecular phylogeny, they were still treated as subgenera of Entoloma s.l. temporarily, because accepting them as genera will make Entoloma s.l. paraphyletic.

Species Diversity in the Braconid Wasp Genus Allorhogas (Doryctinae) Associated With Cynipid Galls on Live Oaks (Quercus: Fagaceae) Using Natural History, Phylogenetics, and Morphology

2020 ◽  
Vol 4 (5) ◽  
Author(s):  
Ernesto Samacá-Sáenz ◽  
Scott P Egan ◽  
Alejandro Zaldívar-Riverón
Keyword(s):  
New Species ◽  
Species Diversity ◽  
Species Interactions ◽  
Human Life ◽  
Nuclear Data ◽  
Morphological Data ◽  
Molecular Evidence ◽  
Wasp Species ◽  
Genetic Lineages ◽  
Braconid Wasp

Abstract The discovery of new biodiversity, during an age of unprecedented extinction, is vital for all the life sciences and the quality of human life. One ecologically and economically important group that requires attention is the hymenopteran family Braconidae, which is estimated to include thousands of undescribed species. Here we assessed the genetic structure and species diversification in the braconid wasp genus Allorhogas Gahan (Doryctinae) that were reared from galls of five cynipid wasp species associated with three live oak species (Fagaceae: Quercus: subsection Virentes) in the southeastern United States. We explored genetic variation in the single-locus barcoding COI region of the mitochondrial DNA (mtDNA), and conducted analyses with different DNA sequence-based species delimitation approaches both for the above marker and genome-wide nuclear data using ultraconserved elements (UCEs). We found high variation in the mtDNA barcoding region among specimens of Allorhogas reared from galls made by different cynipid species in distinct plant organs and among specimens reared from the same type of gall from two separate geographic regions. In addition, our analyses of mtDNA and multilocus nuclear data were concordant in consistently delimiting at least five genetic lineages. We combined this molecular evidence with morphological data to describe four new species and redescribe the type species of the genus, Allorhogas gallicola Gahan, which exhibited similar morphological, ecological, and biogeographic characteristics to the four new species. This study highlights the importance of carrying detailed rearing surveys to uncover the intricate species interactions and species diversity that is present in gall-former systems.

scholarly journals A revised taxonomy of Asian snail-eating snakes Pareas (Squamata, Pareidae): evidence from morphological comparison and molecular phylogeny

ZooKeys ◽  
2020 ◽  
Vol 939 ◽  
pp. 45-64
Author(s):  
Ping Wang ◽  
Jing Che ◽  
Qin Liu ◽  
Ke Li ◽  
Jie Qiong Jin ◽  
...  
Keyword(s):  
New Species ◽  
Bayesian Inference ◽  
Molecular Phylogeny ◽  
Morphological Data ◽  
Multivariate Morphometrics ◽  
Cryptic Diversity ◽  
Cyt B ◽  
Morphological Comparison ◽  
The Family ◽  
Two New Species

The Asian snail-eating snakes Pareas is the largest genus of the family Pareidae (formerly Pareatidae), and widely distributed in Southeast Asia. However, potential diversity remains poorly explored due to their highly conserved morphology and incomplete samples. Here, on basis of more extensive sampling, interspecific phylogenetic relationships of the genus Pareas were reconstructed using two mitochondrial fragments (cyt b and ND4) and two nuclear genes (c-mos and Rag1), and multivariate morphometrics conducted for external morphological data. Both Bayesian Inference and Maximum Likelihood analyses consistently showed that the genus Pareas was comprised of two distinct, monophyletic lineages with moderate to low support values. Based on evidences from molecular phylogeny and morphological data, cryptic diversity of this genus was uncovered and two new species were described. In additional, the validity of P. macularius is confirmed.

scholarly journals Two New Species of Taygetina With a Possible Case of ‘Juxta Loss’ in Butterflies (Lepidoptera: Nymphalidae: Satyrinae)

2019 ◽  
Vol 3 (6) ◽  
Author(s):  
Shinichi Nakahara ◽  
Pável Matos-Maraví ◽  
Eduardo P Barbosa ◽  
Keith R Willmott ◽  
Gerardo Lamas ◽  
...  
Keyword(s):  
New Species ◽  
Species Diversity ◽  
Molecular Phylogeny ◽  
Rare Case ◽  
Unusual Case ◽  
Single Species ◽  
Character State ◽  
State Change ◽  
Two New Species ◽  
A New Species

Abstract The male genitalic characters of Hexapoda are well known for their great taxonomic and systematic value. Despite insect male genitalia displaying large diversity, variation, and modification across orders, some structures are consistently present, and such characters can serve as the basis for discussion regarding homology. In the order Lepidoptera, a male genitalic structure widely known as the ‘juxta’ is present in many taxa and absence or modification of this character can be phylogenetically informative at the generic or higher level. We here focus on the systematics of the so-called ‘Taygetis clade’ within the nymphalid subtribe Euptychiina, and report an unusual case of ‘juxta loss’ in a single species, Taygetina accacioi Nakahara & Freitas, n. sp., a new species from Brazil named and described herein. Additionally, we describe another west Amazonian Taygetina Forster, 1964 species, namely Taygetina brocki Lamas & Nakahara, n. sp., in order to better document the species diversity of Taygetina. Our most up-to-date comprehensive molecular phylogeny regarding ‘Taygetis clade’ recovered these two species as members of a monophyletic Taygetina, reinforcing the absence of juxta being a character state change occurring in a single lineage, resulting in an apomorphic condition, which we report here as a rare case in butterflies (Papilionoidea).

Morphological and phylogenetic study of the Western Atlantic Crepidula plana complex (Caenogastropoda, Calyptraeidae), with description of three new species from Brazil

Zootaxa ◽  
2006 ◽  
Vol 1112 (1) ◽  
pp. 1 ◽  
Author(s):  
LUIZ RICARDO L. SIMONE
Keyword(s):  
New Species ◽  
Morphological Data ◽  
Phylogenetic Study ◽  
Good Resolution ◽  
Western Atlantic ◽  
Informal Group ◽  
Phylogenetic Studies ◽  
History Of ◽  
Three New Species ◽  
Good Number

A detailed morphological study was performed in the following species of Crepidula: 1) C. margarita new species from Venezuela; 2) C. plana Say, 1822; 3) C. atrasolea Collin, 2000; 4) C. depressa Say, 1822, from Florida, USA; 5) C. pyguaia, new species from Santa Catarina coast, Brazil; 6) C. carioca, new species from Rio de Janeiro coast, Brazil. Additionally, five other species anatomically studied in Simone (2002) were also included: 7) C. argentina Simone, Pastorino & Penchaszadeh, 2000, from Argentina; 8) C. glauca Say, 1822, from Venezuela; 9) C. fornicata (Linné, 1758), from Mediterranean and Florida; 10) C. protea Orbigny, from S.E. Brazil; 11) C. aff. plana, here described as C. intratesta new species, from S.E. Brazil; 12) C. cachimilla Cledón Simone & Penchaszadeh, 2004, from Patagonia. The 46 characters (106 states) for these species were phylogenetically analyzed and a single cladogram was obtained (length: 93, CI: 65; RI: 64) as follows: (Crepidula cachimilla (C. argentina ((C. carioca C. pyguaia) ((C. fornicata (C. intratesta C. protea)) ((C. glauca C. margarita) (C. plana (C. atrasolea C. depressa))))))). Two outgroups were used: Bostrycapulus aculeatus (Gmelin, 1791), which most authors consider a Crepidula (operationally analyzed as part of the ingroup), and the remaining calyptraeoideans studied by the author. The monophyly of the ingroup is confirmed, supported by 25 morphological synapomorphies. Although the ingroup is fully resolved, no clades are formally named, because the phylogeny is still considered provisional. Most studied species belong to an informal group called “Crepidula plana-complex”, but it is not monophyletic, since C. fornicata, which does not belong to this complex, is part of the ingroup. This study demonstrates that detailed morphological data are useful in phylogenetic studies even at the level of closely related/sibling species, resulting in cladogram with good resolution and a good number of shared, analyzable characters. A biogeographic analysis is also performed considering the distribution of each species under the light of the obtained cladogram, a clear ascension from south to north is the main pattern of the evolutionary history of these species. Further comments on the systematics of Crepidula aplysioides Reeve and C. convexa Say is also performed.

Morphological and molecular evidence of some Minuartia species (Caryophyllaceae) from Turkey, with a desciption of a new species

Phytotaxa ◽  
2019 ◽  
Vol 391 (2) ◽  
pp. 122 ◽  
Author(s):  
MURAT KOÇ ◽  
ERGIN HAMZAOĞLU ◽  
AHMET AKSOY
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Related Species ◽  
Phylogenetic Relationships ◽  
Morphological Data ◽  
Molecular Evidence ◽  
A New Species

The genus Minuartia is represented in Turkey by 34 taxa. Some interesting specimens were collected from Antalya province, and examined. These specimens resemble Minuartia meyeri, and M. multinervis from which differ by characters (macro-, and micromorphological) of inflorescence, alar pedicels, petals, sepals, capsules and seeds. Moreover, by using the DNA sequences of the ITS genes, phylogenetic relationships between this collected species, and the related species were investigated. As a result of the evaluation of molecular, and morphological data, we proposed to described the population from Antalya as a new species for the science. A description, pictures, distribution, habitat, and IUCN category are given.

Observations on Malenchus geraerti n. sp. (Rhabditida: Tylenchidae), a morphological and molecular phylogenetic study

Zootaxa ◽  
2018 ◽  
Vol 4369 (3) ◽  
pp. 406 ◽  
Author(s):  
MAJID PEDRAM ◽  
MAHYA SOLEYMANZADEH ◽  
EBRAHIM POURJAM ◽  
MAHYAR MOBASSERI
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Small Subunit ◽  
Phylogenetic Study ◽  
Molecular Characteristics ◽  
Lsu Rdna ◽  
Northern Iran ◽  
Lateral Field ◽  
Molecular Phylogenetic ◽  
Short Body

Malenchus geraerti n. sp., recovered from natural regions of northern Iran, is described and illustrated based on morphological, morphometric and molecular data. The new species is characterized by having females with a short body, an anteriorly wide S-shaped amphidial opening narrowing posteriorly, cuticle with prominent annuli, lateral field a plain band with smooth margins, muscular metacorpus with well-developed valve and corresponding plates, spermatheca filled with small spheroid sperm cells, vulva sunken in body with large epiptygma and no flap, and conical tail tapering gradually to a more or less pointed tip. Males of the new species are characterized by having a short body, tylenchoid spicules, adcloacal bursa with smooth margin and tail similar to that of the female. Morphologically, the new species is similar to five known species of the genus: M. fusiformis, M. machadoi, M. pachycephalus, M. solovjovae and M. undulatus. It most closely resembles M. pachycephalus, but as a cryptic species it can be differentiated using morphological and molecular characteristics. Comparisons with the four other aforementioned species are also discussed. Molecular phylogenetic studies using partial sequences of small and large subunit ribosomal DNA fragments reveal that the new species forms a clade with the species M. neosulcus in the small subunit (SSU) rDNA, and two species of Lelenchus in the large subunit (LSU) rDNA tree. 

A new species of Aoruroides Travassos & Kloss, 1958 (Nematoda: xyuridomorpha: Thelastomatidae) parasite of the wood-burrowing cockroach Panesthia angustipennis spadica (Shiraki, 1906) (Blattodea: Blaberidae: Panesthiinae) from Japan with comments on the validity of the genus Aoruroides

Zootaxa ◽  
2019 ◽  
Vol 4712 (3) ◽  
pp. 365-376
Author(s):  
JANS MORFFE ◽  
NAYLA GARCÍA ◽  
KOICHI HASEGAWA ◽  
RAMON A. CARRENO
Keyword(s):  
New Species ◽  
Body Length ◽  
Excretory Pore ◽  
Nerve Ring ◽  
The Body ◽  
Morphological Data ◽  
Lsu Rdna ◽  
Japanese Archipelago ◽  
The Japanese Archipelago ◽  
A New Species

Aoruroides chubudaigaku n. sp. (Nematoda: Oxyuridomorpha: Thelastomatidae) is described from the wood-burrowing cockroach Panesthia angustipennis spadica (Shiraki, 1906) (Blattodea: Blaberidae: Panesthiinae) from Aichi prefecture, Japan. Females of A. chubudaigaku n. sp. are similar to A. costaricensis Carreno & Tuhela, 2011 by the position of the nerve ring at level of the first third of the isthmus, the body length and the comparative measurements of the oesophagus and tail. They differ by the position of the excretory pore and the vulva. The males of A. chubudaigaku n. sp. are characterized by the absence of ornamentations in the cervical cuticle and the nerve ring located at the posterior third of the corpus. In addition, the males of the new species can be differentiated by the length of the body and the comparative lengths of the oesophagus and the tail. The phylogeny of A. chubudaigaku n. sp. is inferred by the D2-D3 domains of the LSU rDNA and the validity of the genus Aoruroides Travassos & Kloss, 1958 is discussed on the basis of both molecular and morphological data. This constitutes the first species of the genus Aoruroides described from the Japanese archipelago. 

Molecular and morphological data place Blarneya in Tylophoron (Arthoniaceae)

2011 ◽  
Vol 43 (4) ◽  
pp. 345-356 ◽  
Author(s):  
Damien ERTZ ◽  
Frank BUNGARTZ ◽  
Paul DIEDERICH ◽  
Leif TIBELL
Keyword(s):  
Molecular Phylogeny ◽  
Molecular Analysis ◽  
Morphological Data ◽  
Molecular Evidence ◽  
Dry Forests ◽  
Single Locality

AbstractBased on morphological, anatomical, chemical, ecological and molecular evidence, Blarneya is synonymized here with Tylophoron. The molecular phylogeny derived from sequences obtained from sporodochia of Blarneya places this genus, described to accommodate an anamorphic lichen with white cushion-shaped sporodochia, within Tylophoron. This conclusion is further supported by the discovery of Tylophoron-type ascomata emerging directly from thalli with Blarneya-type sporodochia and producing identical hyaline conidia. In one specimen pycnidia were also observed. This represents a surprising variety of morphologically different conidiomata. A different anamorphic type was previously reported from Tylophoron, and this is confirmed here by molecular analysis for T. moderatum: besides thalli with ascomata this species has anamorphic thalli with an irregularly delimited brown sporodochial felt and brown conidia. Ascomata are not known from these entirely anamorphic thalli, whereas they do occur infrequently in Tylophoron species with Blarneya-type sporodochia. A key to all currently accepted species of Tylophoron is provided. In addition to the corticolous Tylophoron hibernicum, confined to humid forests, two saxicolous species with Blarneya-type sporodochia are described here as new: T. galapagoense, known only from Galapagos, differs from T. hibernicum by a thicker, more compact, beige rather than white, more strongly C+ red thallus, growing below sheltered rock overhangs in dry forests; T. stalactiticum has a C− thallus with stipitate, white, C+ red sporodochia; the species is known only from a single locality in Tenerife, on a large slope with volcanic boulders.

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