scholarly journals Fermentative Potential of Native Yeast Candida famata for Prokupac Grape Must Fermentation

Agriculture ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 358
Author(s):  
Stojan Mančić ◽  
Bojana Danilović ◽  
Marko Malićanin ◽  
Sandra Stamenković Stojanović ◽  
Nada Nikolić ◽  
...  
Keyword(s):  
Perfect Match ◽  
Control Sample ◽  
Fermentation Performance ◽  
Good Tolerance ◽  
Grape Must ◽  
Candida Famata ◽  
Organic Acid Profile ◽  
Sequential Fermentation ◽  
Grape Must Fermentation ◽  
Selection Of

The fermentative potential of native Candida famata isolates from wild and cultivated blackberries was evaluated for potential application in Prokupac grape must fermentation. 5 isolates, out of a total 22 isolated yeasts, were identified as C. famata. After the initial screening of fermentative performances, microfermentation was performed in a sterile grape must. Produced samples were analyzed using the HPLC technique. All isolates showed an ability to grow at lower temperatures, good tolerance to 7% ethanol and 300 ppm of SO2. C. famata isolates WB-1, WB-2 and W-5 had similar fermentation performance, but WB-1 isolate was chosen for validation at a laboratory-scale level according to a pleasant, fruity aroma, highest fermentative vigor and power, good organic acid profile and the highest level of ethanol and glycerol produced in micro-vinification experiments. Good enological performance of selected C. famata WB-1 isolate is confirmed by higher level of glycerol, lower level of ethanol and acetic acid in wine samples produced in pure and sequential fermentation, when compared to the control sample. Throughout the selection of C. famata yeasts with good enological potential, this work gives a contribution in the area of precision enology, aiming to find a perfect match between non-exploited yeasts and “autochthonous” grape cultivar Prokupac.

scholarly journals Assessing the Oenological Potential of Nakazawaea ishiwadae, Candida railenensis and Debaryomyces hansenii Strains in Mixed-Culture Grape Must Fermentation with Saccharomyces cerevisiae

Fermentation ◽  
2020 ◽  
Vol 6 (2) ◽  
pp. 49 ◽  
Author(s):  
Niel van Wyk ◽  
Isak S. Pretorius ◽  
Christian von Wallbrunn
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Mixed Culture ◽  
Debaryomyces Hansenii ◽  
Fermentation Performance ◽  
Acetate Esters ◽  
Grape Must ◽  
Saccharomyces Yeast ◽  
Grape Must Fermentation

Recently, there has been a growing interest in the role of non-Saccharomyces yeast (NSY) as a coculturing partner with Saccharomyces cerevisiae during grape must fermentation. We investigated three new strains, namely Nakazawaea ishiwadae, Candida railenensis and Debaryomyces hansenii, for their oenological potential in mixed-culture micro-vinifications with S. cerevisiae Vin13 using Muscaris grape must. None of the NSY strains impeded the fermentation performance as all the mixed-culture experiments finished at the same time. Coculturing with N. ishiwadae yielded significantly higher concentrations of ethyl and acetate esters in the final wine product. Apart from higher acetic acid levels, wines produced with C. railenensis and D. hansenii yielded much lower esters concentrations. The concentrations of certain terpenes and norisoprenoids were also significantly modulated in the mixed-culture fermentations. This study reveals the rarely reported species of N. ishiwadae as a promising coculturing partner for increasing aroma-active compounds in a wine.

scholarly journals Maintenance with Trabectedin in the Treatment of Platinum-Sensitive Recurrent Ovarian Cancer

2019 ◽  
Vol 12 (2) ◽  
pp. 447-455 ◽  
Author(s):  
Eva María Guerra Alía ◽  
Cayetano Sempere Ortega ◽  
Alfonso Cortés Salgado ◽  
Concepción Sanchez Martínez ◽  
Julio Galindo Álvarez ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Pegylated Liposomal Doxorubicin ◽  
Abdominal Distension ◽  
Recurrent Ovarian Cancer ◽  
Response To Treatment ◽  
Case Presentation ◽  
Good Tolerance ◽  
Platinum Sensitive ◽  
Platinum Based Chemotherapy ◽  
Selection Of

Ovarian cancer is the seventh most common type of cancer and the fifth leading cause of cancer death among women worldwide. The current usual therapeutic approach in this disease includes optimal cytoreductive therapy followed by platinum-based adjuvant chemotherapy, along with neoadjuvant chemotherapy prior to surgery in selected cases. The platinum-free interval (PFI) continues to be the most useful tool to assist in the selection of the subsequent therapy and to predict response to treatment. The combination of trabectedin and pegylated liposomal doxorubicin (PLD) is useful in patients with partially platinum-sensitive recurrent ovarian cancer, in patients who have previously received two or more platinum-based chemotherapy regimens, in patients who have already experienced a platinum-induced hypersensitivity reaction and in patients who have previously failed to respond to a platinum-based treatment. Case Presentation: A 64-years-old postmenopausal woman with pain, abdominal distension, and an altered intestinal transit and with partially platinum-sensitive recurrent ovarian cancer, was successfully treated with a second line of trabectedin chemotherapy in combination with PLD, followed by trabectedin in monotherapy. This case proves the effectiveness of the combination of trabectedin and PLD and demonstrates how the administration of trabectedin, even in monotherapy, allows to maintain an adequate clinical response with good tolerance to the treatment during more than two years of drug administration.

Volatiles Formation from Grape Must Fermentation Using a Cryophilic and Thermotolerant Yeast

2012 ◽  
Vol 167 (5) ◽  
pp. 1183-1198 ◽  
Author(s):  
Nikolaos Kopsahelis ◽  
Loulouda Bosnea ◽  
Maria Kanellaki ◽  
Athanasios A. Koutinas
Keyword(s):  
Thermotolerant Yeast ◽  
Grape Must ◽  
Grape Must Fermentation

scholarly journals New library of aminosulfonyl-tagged Hoveyda–Grubbs type complexes: Synthesis, kinetic studies and activity in olefin metathesis transformations

2010 ◽  
Vol 6 ◽  
pp. 1159-1166 ◽  
Author(s):  
Etienne Borré ◽  
Frederic Caijo ◽  
Christophe Crévisy ◽  
Marc Mauduit
Keyword(s):  
Olefin Metathesis ◽  
Kinetic Studies ◽  
Ring Closing Metathesis ◽  
Good Tolerance ◽  
Cross Metathesis ◽  
Activity Enhancement ◽  
Selection Of

Seven novel Hoveyda–Grubbs precatalysts bearing an aminosulfonyl function are reported. Kinetic studies indicate an activity enhancement compared to Hoveyda’s precatalyst. A selection of these catalysts was investigated with various substrates in ring-closing metathesis of dienes or enynes and cross metathesis. The results demonstrate that these catalysts show a good tolerance to various chemical functions.

scholarly journals Directed cultivation of Chlorella sorokiniana to increase carotenoid synthesis

2020 ◽  
Vol 81 (4) ◽  
pp. 34-39
Author(s):  
T. A. Kuznetsova ◽  
M. S. Nikitina ◽  
A. D. Sevastyanova
Keyword(s):  
Ultraviolet Irradiation ◽  
Spectral Region ◽  
Control Sample ◽  
Light Flux ◽  
Chlorella Sorokiniana ◽  
Fluorescent Light ◽  
Ultraviolet Exposure ◽  
Stabilization Phase ◽  
High Antioxidant Activity ◽  
Selection Of

Chlorella sorokiniana is a promising species for cultivation both in the laboratory cultivator and on an industrial scale. Its biomass is the source of many valuable components, including plastid pigments, which have high antioxidant activity. The metabolism of Chlorella sorokiniana is subject to change under the influence of various cultivation conditions. With dosed ultraviolet radiation, a compensatory increase in the synthesis of carotenoids is possible, which prevents oxidative stress. The cultivation of C. sorokiniana (strain 211-8k) was carried out in various conditions of illumination: the control version – illumination with a fluorescent lamp; option 1 – dosed periodic ultraviolet exposure every day for 15 min (spectral region of the light flux 280-315 nm (UV-B), intensity 1300 Lux) and further illumination with a fluorescent light; option 2 – ultraviolet irradiation for 30 min (spectral region of the light flux 280-315 nm (UV-B), intensity 1300 Lux) in the stabilization phase. Periodic ultraviolet irradiation negatively affects the growth of C. sorokiniana population, which manifests itself only on the 9th day, the biomass yield is significantly reduced. A single UV exposure for 30 minutes leads to a slight decrease in the yield of air-dried biomass, which can be compensated with a further increase in population. Periodic ultraviolet exposure leads to an increase in the synthesis of carotenoids, the yield in terms of dry biomass exceeds the control sample by an average of 30%. A single ultraviolet irradiation for 30 minutes in the stabilization phase leads to a decrease in the content of both chlorophyll and carotenoids in the biomass. Microscopic examination of microalgae populations showed that ultraviolet exposure leads to the appearance of cells with signs of apoptosis: large cells with large vacuoles, a condensed nucleus, and bleached chloroplast. A further direction of the study is the selection of conditions allowing to increase the yield of carotenoids with minimal loss of microalgae biomass.

scholarly journals Applicability of Saccharomyces cerevisiae Strains for the Production of Fruit Wines Using Cocoa Honey Complemented with Cocoa Pulp

2021 ◽  
Vol 60 (2) ◽  
Author(s):  
Bárbara Teodora Andrade Koelher ◽  
Soraya Maria Moreira de Souza ◽  
Andréa Miura da Costa ◽  
Elizama Aguiar-Oliveira
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Experimental Approach ◽  
Inoculum Size ◽  
Added Value ◽  
Fruit Pulp ◽  
Fermentation Performance ◽  
Scientific Contribution ◽  
Commercial Strain ◽  
Sweet Wine ◽  
Selection Of

Research background. Cocoa honey (CH) and cocoa pulp (CP) are both fruit pulps highly appreciated but, until now, CH is less processed than CP. In this work, it was investigated the applicability of strains of S. cerevisiae to ferment CH complemented with CP, to obtain fruit wines and improve CH commercialization. Experimental approach. The selection of a strain, previously isolated from cachaçaria distilleries in Brazil, took place based on its fermentation performance. The conditions for fermentation with S. cerevisiae L63 were then studied in relation to: volumetric proportion (φCH) of CH (complemented with CP), sucrose addition (γsuc), temperature (T) and inoculum size (No). The best conditions were applied in order to obtain fermentation profiles. Results and conclusions. S. cerevisiae L63 (No=107–108 cell/mL) is capable to ferment φCH of 90 and 80 % (V/V) for 24 or 48 h with γsuc of 50 and 100 g/L at T=28–30 °C resulting in wines with ethanol contents from 8 to 14 % (V/V). Additionally, the φCH=90 % (V/V) wine resulted in the lowest residual sugar concentration (<35 g/L) than the φCH=80 % (V/V) wine (~79 g/L) which could be classified as a sweet wine. In general, S. cerevisiae L63 resulted in a similar fermentation performance than a commercial strain tested, indicating its potential for fruit pulp fermentation. Novelty and scientific contribution. Therefore, S. cerevisiae L63 is capable to ferment CH complemented with CP to produce fruit wines with good commercial potentials that may also benefit small cocoa producers by presenting a product with greater added value.

scholarly journals Selection of Wine Saccharomyces cerevisiae Strains and Their Screening for the Adsorption Activity of Pigments, Phenolics and Ochratoxin A

Fermentation ◽  
2020 ◽  
Vol 6 (3) ◽  
pp. 80
Author(s):  
Andrea Pulvirenti ◽  
Luciana De Vero ◽  
Giuseppe Blaiotta ◽  
Rossana Sidari ◽  
Giovanna Iosca ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Phenolic Compounds ◽  
Ochratoxin A ◽  
Red Wines ◽  
Wine Yeasts ◽  
Fermentation Performance ◽  
Adsorption Ability ◽  
Adsorption Activity ◽  
Safety Hazard ◽  
Selection Of

Ochratoxin A is a dangerous mycotoxin present in wines and is considered the principal safety hazard in the winemaking process. Several authors have investigated the ochratoxin A adsorption ability of Saccharomyces cerevisiae yeasts, and specifically selected strains for this desired trait. In the present work, a huge selection of wine yeasts was done starting from Portuguese, Spanish and Italian fermenting musts of different cultivars. Firstly, 150 isolates were collected, and 99 non-redundant S. cerevisiae strains were identified. Then, the strains were screened following a multi-step approach in order to select those having primary oenological traits, mainly (a) good fermentation performance, (b) low production of H2S and (c) low production of acetic acid. The preselected strains were further investigated for their adsorption activity of pigments, phenolic compounds and ochratoxin A. Finally, 10 strains showed the desired features. The goal of this work was to select the strains capable of absorbing ochratoxin A but not pigments and phenolic compounds in order to improve and valorise both the quality and safety of red wines. The selected strains are considered good candidates for wine starters, moreover, they can be exploited to obtain a further enhancement of the specific adsorption/non-adsorption activity by applying a yeast breeding approach.

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