scholarly journals Applicability of Saccharomyces cerevisiae Strains for the Production of Fruit Wines Using Cocoa Honey Complemented with Cocoa Pulp

2021 ◽  
Vol 60 (2) ◽  
Author(s):  
Bárbara Teodora Andrade Koelher ◽  
Soraya Maria Moreira de Souza ◽  
Andréa Miura da Costa ◽  
Elizama Aguiar-Oliveira
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Experimental Approach ◽  
Inoculum Size ◽  
Added Value ◽  
Fruit Pulp ◽  
Fermentation Performance ◽  
Scientific Contribution ◽  
Commercial Strain ◽  
Sweet Wine ◽  
Selection Of

Research background. Cocoa honey (CH) and cocoa pulp (CP) are both fruit pulps highly appreciated but, until now, CH is less processed than CP. In this work, it was investigated the applicability of strains of S. cerevisiae to ferment CH complemented with CP, to obtain fruit wines and improve CH commercialization. Experimental approach. The selection of a strain, previously isolated from cachaçaria distilleries in Brazil, took place based on its fermentation performance. The conditions for fermentation with S. cerevisiae L63 were then studied in relation to: volumetric proportion (φCH) of CH (complemented with CP), sucrose addition (γsuc), temperature (T) and inoculum size (No). The best conditions were applied in order to obtain fermentation profiles. Results and conclusions. S. cerevisiae L63 (No=107–108 cell/mL) is capable to ferment φCH of 90 and 80 % (V/V) for 24 or 48 h with γsuc of 50 and 100 g/L at T=28–30 °C resulting in wines with ethanol contents from 8 to 14 % (V/V). Additionally, the φCH=90 % (V/V) wine resulted in the lowest residual sugar concentration (<35 g/L) than the φCH=80 % (V/V) wine (~79 g/L) which could be classified as a sweet wine. In general, S. cerevisiae L63 resulted in a similar fermentation performance than a commercial strain tested, indicating its potential for fruit pulp fermentation. Novelty and scientific contribution. Therefore, S. cerevisiae L63 is capable to ferment CH complemented with CP to produce fruit wines with good commercial potentials that may also benefit small cocoa producers by presenting a product with greater added value.

scholarly journals Selection of Wine Saccharomyces cerevisiae Strains and Their Screening for the Adsorption Activity of Pigments, Phenolics and Ochratoxin A

Fermentation ◽  
2020 ◽  
Vol 6 (3) ◽  
pp. 80
Author(s):  
Andrea Pulvirenti ◽  
Luciana De Vero ◽  
Giuseppe Blaiotta ◽  
Rossana Sidari ◽  
Giovanna Iosca ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Phenolic Compounds ◽  
Ochratoxin A ◽  
Red Wines ◽  
Wine Yeasts ◽  
Fermentation Performance ◽  
Adsorption Ability ◽  
Adsorption Activity ◽  
Safety Hazard ◽  
Selection Of

Ochratoxin A is a dangerous mycotoxin present in wines and is considered the principal safety hazard in the winemaking process. Several authors have investigated the ochratoxin A adsorption ability of Saccharomyces cerevisiae yeasts, and specifically selected strains for this desired trait. In the present work, a huge selection of wine yeasts was done starting from Portuguese, Spanish and Italian fermenting musts of different cultivars. Firstly, 150 isolates were collected, and 99 non-redundant S. cerevisiae strains were identified. Then, the strains were screened following a multi-step approach in order to select those having primary oenological traits, mainly (a) good fermentation performance, (b) low production of H2S and (c) low production of acetic acid. The preselected strains were further investigated for their adsorption activity of pigments, phenolic compounds and ochratoxin A. Finally, 10 strains showed the desired features. The goal of this work was to select the strains capable of absorbing ochratoxin A but not pigments and phenolic compounds in order to improve and valorise both the quality and safety of red wines. The selected strains are considered good candidates for wine starters, moreover, they can be exploited to obtain a further enhancement of the specific adsorption/non-adsorption activity by applying a yeast breeding approach.

scholarly journals Reducing glucoamylase usage for commercial-scale ethanol production from starch using glucoamylase expressing Saccharomyces cerevisiae

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Xin Wang ◽  
Bei Liao ◽  
Zhijun Li ◽  
Guangxin Liu ◽  
Liuyang Diao ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Nitrogen Source ◽  
Culture Media ◽  
Production Condition ◽  
Cassava Starch ◽  
Inoculum Size ◽  
Acid Protease ◽  
Fermentation Performance ◽  
Commercial Scale

AbstractThe development of yeast that converts raw corn or cassava starch to ethanol without adding the exogenous α-amylase and/or glucoamylase would reduce the overall ethanol production cost. In this study, two copies of codon-optimized Saccharomycopsis fibuligera glucoamylase genes were integrated into the genome of the industrial Saccharomyces cerevisiae strain CCTCC M94055, and the resulting strain CIBTS1522 showed comparable basic growth characters with the parental strain. We systemically evaluated the fermentation performance of the CIBTS1522 strain using the raw corn or cassava starch at small and commercial-scale, and observed that a reduction of at least 40% of the dose of glucoamylase was possible when using the CIBTS1522 yeast under real ethanol production condition. Next, we measured the effect of the nitrogen source, the phosphorous source, metal ions, and industrial microbial enzymes on the strain’s cell wet weight and ethanol content, the nitrogen source and acid protease showed a positive effect on these parameters. Finally, orthogonal tests for some other factors including urea, acid protease, inoculum size, and glucoamylase addition were conducted to further optimize the ethanol production. Taken together, the CIBTS1522 strain was identified as an ideal candidate for the bioethanol industry and a better fermentation performance could be achieved by modifying the industrial culture media and condition.

Analysis of the Most Valued Organization to Work for in Spain, According to Nationality and Location

2019 ◽  
Vol 5 (2) ◽  
pp. 83-99
Author(s):  
Francisco Jesús Ferreiro Seoane ◽  
Manuel Octavio Del Campo Villares
Keyword(s):  
Work Environment ◽  
Talent Management ◽  
Point Of View ◽  
Added Value ◽  
Quantitative Character ◽  
Autonomous Communities ◽  
Professional Performance ◽  
Significant Relationships ◽  
Pearson Correlations ◽  
Selection Of

Background: The objective of this article is to analyse if there are significant relationships between the most valuable companies operating in Spain regarding professional performance, according to nationality and location within their Autonomous Communities or any superior aggrupation. To do that, a sample of 100 companies has been selected. Methods: The methodology followed is based on the selection of the 100 highestvalued companies from the point of view of Human Resources’ policy for the period 2013-2016 and through the measurement of six factors: Talent Management, Retribution, Work environment, CSR, Training and Employees’ perception, and classified by nationality and location. The study was based on 12 hypotheses, using the Unifactorial Variance’s Analysis, Pearson correlations and regressions. One limitation could be the fact that this study refers to a particular period, focusing on Spain and the variables mentioned, based on questionnaires. The added value of this work lies on the newness as it has a quantitative character, and on the fact that most of the hypotheses do not comply. Results and Conclusion: This allows to deny certain beliefs that affirm that European and American companies operating in Spain are more attractive than the Spanish or the Mediterranean ones.

Big Data in the Field of Civil Security Research

2016 ◽  
Vol 7 (1) ◽  
pp. 54-64 ◽  
Author(s):  
Julia Gonschorek ◽  
Anja Langer ◽  
Benjamin Bernhardt ◽  
Caroline Räbiger
Keyword(s):  
Big Data ◽  
Temporal Distribution ◽  
Tactical Planning ◽  
Fire Departments ◽  
Prevention Measures ◽  
Scientific Contribution ◽  
Security Research ◽  
Geovisual Analytics ◽  
The University ◽  
Selection Of

This article gives insight in a running dissertation at the University in Potsdam. Point of discussion is the spatial and temporal distribution of emergencies of German fire brigades that have not sufficiently been scientifically examined. The challenge is seen in Big Data: enormous amounts of data that exist now (or can be collected in the future) and whose variables are linked to one another. These analyses and visualizations can form a basis for strategic, operational and tactical planning, as well as prevention measures. The user-centered (geo-) visualization of fire brigade data accessible to the general public is a scientific contribution to the research topic 'geovisual analytics and geographical profiling'. It may supplement antiquated methods such as the so-called pinmaps as well as the areas of engagement that are freehand constructions in GIS. Considering police work, there are already numerous scientific projects, publications, and software solutions designed to meet the specific requirements of Crime Analysis and Crime Mapping. By adapting and extending these methods and techniques, civil security research can be tailored to the needs of fire departments. In this paper, a selection of appropriate visualization methods will be presented and discussed.

scholarly journals Recruitment of Employees—Assumptions of the Risk Model

Risks ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 55
Author(s):  
Halina Sobocka-Szczapa
Keyword(s):  
Risk Model ◽  
Model Identification ◽  
Added Value ◽  
Competitive Advantages ◽  
Recruitment Process ◽  
Process Risk ◽  
Model Formation ◽  
Risk Determinants ◽  
Final Selection ◽  
Selection Of

The aim of this article is to present the risk model premises related to worker recruitment. Recruitment affects the final selection of workers, whose activities contribute to corporate competitive advantages. Hiring unfavorable workers can influence the results produced by an organization. This risk mostly affects situations when searching for workers via the external labor market, although it can also affect internal recruitment. Therefore, it is necessary to attempt to identify recruitment risk determinants and classify their meaning in such processes. Model formation has both theoretical and intuitive characteristics. Model dependencies and their characteristics are identified in this paper. We attempted to assess the usability of the risk model for economic praxis. The analyses and results provide a model identification of dependencies between the factors determining a workers recruitment process and the risk which is caused by this process (employing inadequate workers who do not meet the employer’s expectations). The identification of worker recruitment process determinants should allow for practically reducing the risk of employing an inadequate worker and contribute to the reduction in unfavorable recruitment processes. The added value of this publication is the complex identification of recruitment process risk determinants and dependency formulations in a model form.

scholarly journals Physiological performance of Kazachstania unispora in sourdough environments

2021 ◽  
Vol 37 (5) ◽  
Author(s):  
Dea Korcari ◽  
Giovanni Ricci ◽  
Claudia Capusoni ◽  
Maria Grazia Fortina
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Environmental Factors ◽  
Positive Characteristic ◽  
Carbohydrate Source ◽  
High Osmolarity ◽  
Commercial Strain ◽  
Positive Properties ◽  
Nutritional Mutualism

AbstractIn this work we explored the potential of several strains of Kazachstania unispora to be used as non-conventional yeasts in sourdough fermentation. Properties such as carbohydrate source utilization, tolerance to different environmental factors and the performance in fermentation were evaluated. The K. unispora strains are characterized by rather restricted substrate utilization: only glucose and fructose supported the growth of the strains. However, the growth in presence of fructose was higher compared to a Saccharomyces cerevisiae commercial strain. Moreover, the inability to ferment maltose can be considered a positive characteristic in sourdoughs, where the yeasts can form a nutritional mutualism with maltose-positive Lactic Acid Bacteria. Tolerance assays showed that K. unispora strains are adapted to a sourdough environment: they were able to grow in conditions of high osmolarity, high acidity and in presence of organic acids, ethanol and salt. Finally, the performance in fermentation was comparable with the S. cerevisiae commercial strain. Moreover, the growth was more efficient, which is an advantage in obtaining the biomass in an industrial scale. Our data show that K. unispora strains have positive properties that should be explored further in bakery sector. Graphic abstract

scholarly journals Tuning of a Linear-Quadratic Stabilization System for an Anti-Aircraft Missile

Aerospace ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 48
Author(s):  
Witold Bużantowicz
Keyword(s):  
Analytical Solutions ◽  
Feedback Loop ◽  
Linear Quadratic Regulator ◽  
Added Value ◽  
Tuning Parameter ◽  
Stabilization System ◽  
Linear Quadratic ◽  
Quadratic Stabilization ◽  
Novel Method ◽  
Selection Of

A description is given of an application of a linear-quadratic regulator (LQR) for stabilizing the characteristics of an anti-aircraft missile, and an analytical method of selecting the weighting elements of the gain matrix in feedback loop is proposed. A novel method of LQR tuning via a single parameter ς was proposed and tested. The article supplements and develops the topics addressed in the author’s previous work. Its added value includes the observation that the solutions obtained are symmetric pairs, and that the tuning parameter ς proposed for the designed linear-quadratic regulator enables the selection of suitable parameters for the airframe stabilizing loop for the majority of the analytical solutions of the considered Riccati equation.

A Theoretical and Experimental Approach to E-pH Diagram for the Niobium-Water System

CORROSION ◽  
10.5006/3871 ◽  
2021 ◽  
Author(s):  
Rodney Santandrea ◽  
Simone BRASIL ◽  
Leila Reznik ◽  
Ladimir Carvalho ◽  
Luiz Miranda
Keyword(s):  
Experimental Approach ◽  
Ph Monitoring ◽  
Aqueous Media ◽  
Water System ◽  
Chemical Species ◽  
Polarization Curves ◽  
Buffer Solutions ◽  
Excellent Corrosion Resistance ◽  
Experimental Diagram ◽  
Selection Of

E-pH diagrams are usually built from thermodynamic databases available in the literature or from specific software. However, depending on the conditions and the chemical species defined for elaborating a diagram, it may present completely different immunity, passivation, and corrosion domains. In order to obtain a result closer to a real system, experimental E-pH diagrams can be built from polarization curves obtained in the evaluated conditions. This work discloses the construction of a diagram for the Nb-H&lt;sub&gt;2&lt;/sub&gt;O system at 25°C from theoretical study and the specific selection of chemical species in the solutions through computer simulations. The polarization curves for the construction of the experimental diagram were gathered without the use of buffer solutions and under pH monitoring in the solution bulk throughout all assays. The methodology proposed was considered adequate since, from experimental data, a final result compatible with the classic diagram for the Nb-H&lt;sub&gt;2&lt;/sub&gt;O system and the excellent corrosion resistance of niobium in aqueous media were achieved.

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