Screen Oilseed Rape (Brassica napus) Suitable for Low-Loss Mechanized Harvesting

The main reason for the massive loss of rapeseed in mechanized harvesting is the mismatch between the harvester requirements and the pod shatter resistance and plant branching characteristics. Low pod resistance, entanglement caused by excessive branches, and inconsistent pod maturity are primary mismatch problems. However, studies on rape characteristics by integrating agricultural machinery and agronomy are limited. A total of 15 varieties were planted for research from 2016 to 2018. In this paper, the Two-Degree-of-Freedom (2-DOF) collision method was adopted to evaluate the pod shatter resistance taken from the field, and the plant agronomic characteristics and their correlation were investigated. In 2020, a screened variety of C6009 with higher shatter resistance and suitable plant features for mechanized harvesting was planted in large areas and harvested by machines for verification. The test results demonstrated that the compact plant varieties with high branches might be more favorable for yield and shatter resistance. The field harvest loss of the screened variety was significantly less than that of the control group. It provides a reliable reference for agronomic experts in terms of rape variety improvement and agricultural machinery experts regarding the optimization of rape harvesters.

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Using novel variation in Brassica species to reduce agricultural inputs and improve agronomy of oilseed rape—a case study in pod shatter resistance

Plant Genetic Resources ◽

10.1079/pgr200311 ◽

2003 ◽

Vol 1 (1) ◽

pp. 59-65 ◽

Cited By ~ 2

Author(s):

Colin Morgan ◽

Adrian Bavage ◽

Ian Bancroft ◽

David Bruce ◽

Robin Child ◽

...

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

Gene Pool ◽

Brassica Species ◽

Pod Shattering ◽

Genetic Base ◽

Genetic Potential ◽

Pod Shatter ◽

Novel Variation

AbstractOilseed rape is a very undeveloped crop with regard to efficiency of production and the agronomic practice used to maximize its potential. The genetic potential to modify oilseed rape is limited by the narrow genetic base found within the breeding gene pool, resulting in limited novel variation available for exploitation. Novel variation is, however, present in wild diploid ancestors of oilseed rape and has been made available by developing synthetic Brassica napus. This is illustrated through the use of this material to develop an understanding of pod shattering which is one of the most agronomically important characteristics of the crop. Through a variety of approaches it is shown how progress has been made to understand this trait and how this understanding is being used to improve the crop such that efficiency of production will be enhanced.

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An Epigenetic LTR-retrotransposon insertion in the upstream region of BnSHP1.A9 controls quantitative pod shattering resistance in Brassica napus

10.1101/858407 ◽

2019 ◽

Author(s):

Jia Liu ◽

Rijin Zhou ◽

Wenxiang Wang ◽

Hui Wang ◽

Yu Qiu ◽

...

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

Molecular Mechanisms ◽

Upstream Region ◽

Upstream Sequence ◽

Ltr Retrotransposon ◽

Pod Shattering ◽

Retrotransposon Insertion ◽

Pod Shatter ◽

Positive Effect

AbstractSeed loss resulting from pod shattering is a major problem in oilseed rape (Brassica napus L.) production worldwide. However, the molecular mechanisms underlying pod shatter resistance are not well understood. Here we show that the pod shatter resistance at quantitative trait locus, qSRI.A9.1 is controlled by a SHATTERPROOF1 (SHP1) paralog in B. napus (BnSHP1.A9). Expression analysis by quantitative RT-PCR showed that BnSHP1.A9 was specifically expressed in flower buds, flowers and developing siliques in the oilseed rape line (R1) carrying the qSRI.A9.1 allele with negative effect, but not expressed in any tissue of the line (R2) carrying the positive effect qSRI.A9.1 allele. Transgenic plants constitutively expressing BnSHP1.A9 alleles from pod resistant and pod shattering parental lines showed that both alleles are responsible for pod shattering via promoting lignification of enb layer, which indicated allelic difference of BnSHP1.A9 gene per se is not the causal factor of the QTL. The upstream sequence of BnSHP1.A9 in the promotor region harboring highly methylated long terminal repeat retrotransposon insertion (LTR, 4803bp) in R2 repressed the expression of BnSHP.A9, and thus contributed to the positive effect on pod shatter resistance. Genetic and association analysis revealed that the copia LTR retrotransposon based marker BnSHP1.A9-R2 can be used for breeding for pod shatter resistant varieties and reducing the loss of seed yield in oilseed rape.

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