Using novel variation in Brassica species to reduce agricultural inputs and improve agronomy of oilseed rape—a case study in pod shatter resistance

AbstractOilseed rape is a very undeveloped crop with regard to efficiency of production and the agronomic practice used to maximize its potential. The genetic potential to modify oilseed rape is limited by the narrow genetic base found within the breeding gene pool, resulting in limited novel variation available for exploitation. Novel variation is, however, present in wild diploid ancestors of oilseed rape and has been made available by developing synthetic Brassica napus. This is illustrated through the use of this material to develop an understanding of pod shattering which is one of the most agronomically important characteristics of the crop. Through a variety of approaches it is shown how progress has been made to understand this trait and how this understanding is being used to improve the crop such that efficiency of production will be enhanced.

Download Full-text

An Epigenetic LTR-retrotransposon insertion in the upstream region of BnSHP1.A9 controls quantitative pod shattering resistance in Brassica napus

10.1101/858407 ◽

2019 ◽

Author(s):

Jia Liu ◽

Rijin Zhou ◽

Wenxiang Wang ◽

Hui Wang ◽

Yu Qiu ◽

...

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

Molecular Mechanisms ◽

Upstream Region ◽

Upstream Sequence ◽

Ltr Retrotransposon ◽

Pod Shattering ◽

Retrotransposon Insertion ◽

Pod Shatter ◽

Positive Effect

AbstractSeed loss resulting from pod shattering is a major problem in oilseed rape (Brassica napus L.) production worldwide. However, the molecular mechanisms underlying pod shatter resistance are not well understood. Here we show that the pod shatter resistance at quantitative trait locus, qSRI.A9.1 is controlled by a SHATTERPROOF1 (SHP1) paralog in B. napus (BnSHP1.A9). Expression analysis by quantitative RT-PCR showed that BnSHP1.A9 was specifically expressed in flower buds, flowers and developing siliques in the oilseed rape line (R1) carrying the qSRI.A9.1 allele with negative effect, but not expressed in any tissue of the line (R2) carrying the positive effect qSRI.A9.1 allele. Transgenic plants constitutively expressing BnSHP1.A9 alleles from pod resistant and pod shattering parental lines showed that both alleles are responsible for pod shattering via promoting lignification of enb layer, which indicated allelic difference of BnSHP1.A9 gene per se is not the causal factor of the QTL. The upstream sequence of BnSHP1.A9 in the promotor region harboring highly methylated long terminal repeat retrotransposon insertion (LTR, 4803bp) in R2 repressed the expression of BnSHP.A9, and thus contributed to the positive effect on pod shatter resistance. Genetic and association analysis revealed that the copia LTR retrotransposon based marker BnSHP1.A9-R2 can be used for breeding for pod shatter resistant varieties and reducing the loss of seed yield in oilseed rape.

Download Full-text

Farmers’ perspectives of future management of winter oilseed rape (Brassica napus L.): A case study from north-eastern Germany

European Journal of Agronomy ◽

10.1016/j.eja.2021.126350 ◽

2021 ◽

Vol 130 ◽

pp. 126350

Author(s):

Sabine Andert ◽

Andrea Ziesemer ◽

Han Zhang

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

Winter Oilseed Rape ◽

Brassica Napus L ◽

Eastern Germany ◽

North Eastern ◽

Future Management

Download Full-text

Evaluating agroclimatic constraints and yield gaps for winter oilseed rape (Brassica napus L.) – A case study

Scientific Reports ◽

10.1038/s41598-017-08164-x ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 1

Author(s):

Zhi Zhang ◽

Jianwei Lu ◽

Rihuan Cong ◽

Tao Ren ◽

Xiaokun Li

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

Winter Oilseed Rape ◽

Brassica Napus L ◽

Yield Gaps

Download Full-text

Reconstruction of allopolyploid Brassicas through non-homologous recombination: introgression of resistance to pod shatter in Brassica napus

Genetics Research ◽

10.1017/s0016672300028810 ◽

1990 ◽

Vol 56 (1) ◽

pp. 1-2 ◽

Cited By ~ 26

Author(s):

Shyam Prakash ◽

V. L. Chopra

Keyword(s):

Brassica Napus ◽

Homologous Recombination ◽

Yield Loss ◽

Genetic Resistance ◽

F1 Hybrid ◽

Seed Fertility ◽

Pod Shattering ◽

Pod Shatter ◽

Regular Meiosis

SummaryPod shattering of rapeseed (Brassica napus) causes serious yield loss. Genetic resistance to shattering has been introgressed into B. napus from B. juncea. This followed from allosyndetic pairing between chromosomes of B and C genomes in the interspecific F1 hybrid, B. juncea × B. napus (2n = 37, AABC). The reconstituted B. napus plant showed regular meiosis with 19 bivalents and had pollen and seed fertility of 84 and 23% respectively. An approach is suggested for achieving introgression from monogenomic diploids to digenomic allopolyploids that exploits non-homologous recombination.

Download Full-text

Publisher Correction: Evaluating agroclimatic constraints and yield gaps for winter oilseed rape (Brassica napus L.) – A case study

Scientific Reports ◽

10.1038/s41598-018-20636-2 ◽

2018 ◽

Vol 8 (1) ◽

Cited By ~ 3

Author(s):

Zhi Zhang ◽

Jianwei Lu ◽

Rihuan Cong ◽

Tao Ren ◽

Xiaokun Li

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

Winter Oilseed Rape ◽

Brassica Napus L ◽

Yield Gaps

Download Full-text

Evaluation of the Effectiveness of Pod Sealants in Increasing Pod Shattering Resistance in Oilseed Rape (Brassica napus L.)

Energies ◽

10.3390/en12122256 ◽

2019 ◽

Vol 12 (12) ◽

pp. 2256 ◽

Cited By ~ 1

Author(s):

Dainius Steponavičius ◽

Aurelija Kemzūraitė ◽

Laimis Bauša ◽

Ernestas Zaleckas

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

Seed Yield ◽

Weather Conditions ◽

Control Treatment ◽

Yield Losses ◽

Brassica Napus L ◽

Pod Shattering ◽

Seed Loss ◽

Net Profit

Shattering of pods of oilseed rape (Brassica napus L.) is a major cause of seed yield losses prior to and during harvesting. In order to reduce shattering, researchers have been engaged in the development of special preparations that are known as pod sealants (PS). Despite the fact that there are already developed and commercialized PSs that have only been effective on seed yield preservation under certain environmental conditions, there is still a need to create a more versatile and efficient PS. Currently, the most promising method of controlling pod shattering in oilseed rape is the application of our developed novel acrylic- and trisiloxane-based pod sealant (PS4). The effectiveness of PS4 and three commercial pod sealants (PS1, PS2, and PS3) was assessed in this comparative study. By spraying an oilseed rape crop with PS4, natural seed loss can be reduced by 20–70%, depending on the prevailing weather conditions, and loss of seeds during harvest can be reduced by more than three-fold compared with that by the control treatment. Thus, the overall results demonstrated that by applying a novel pod sealant (PS4) to oilseed rape crops 2 weeks before harvest can increase the net profit margin by €30–€150 ha−1. The life cycle assessment showed that during 2014–2016 oilseed rape cultivation, the largest effect on global warming emission (kg CO2 eq) reduction was experimental sealant PS4, i.e., approximately 17% compared to the control.

Download Full-text

Interspecific Hybridisation between Wild Relatives and Brassica napus to Introduce New Resistance Traits iinto the Oilseed Rape Gene Pool

Czech Journal of Genetics and Plant Breeding ◽

10.17221/6258-cjgpb ◽

2012 ◽

Vol 38 (No. 3-4) ◽

pp. 155-157 ◽

Cited By ~ 9

Author(s):

J. Siemens

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

Gene Pool ◽

Wild Relatives ◽

Interspecific Hybridisation ◽

Resistance Traits

see the fulll text

Download Full-text

Screen Oilseed Rape (Brassica napus) Suitable for Low-Loss Mechanized Harvesting

Agriculture ◽

10.3390/agriculture11060504 ◽

2021 ◽

Vol 11 (6) ◽

pp. 504

Author(s):

Yiren Qing ◽

Yaoming Li ◽

Lizhang Xu ◽

Zheng Ma

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

Degree Of Freedom ◽

Control Group ◽

Test Results ◽

Agricultural Machinery ◽

Low Loss ◽

Agronomic Characteristics ◽

Pod Shatter ◽

Two Degree Of Freedom

The main reason for the massive loss of rapeseed in mechanized harvesting is the mismatch between the harvester requirements and the pod shatter resistance and plant branching characteristics. Low pod resistance, entanglement caused by excessive branches, and inconsistent pod maturity are primary mismatch problems. However, studies on rape characteristics by integrating agricultural machinery and agronomy are limited. A total of 15 varieties were planted for research from 2016 to 2018. In this paper, the Two-Degree-of-Freedom (2-DOF) collision method was adopted to evaluate the pod shatter resistance taken from the field, and the plant agronomic characteristics and their correlation were investigated. In 2020, a screened variety of C6009 with higher shatter resistance and suitable plant features for mechanized harvesting was planted in large areas and harvested by machines for verification. The test results demonstrated that the compact plant varieties with high branches might be more favorable for yield and shatter resistance. The field harvest loss of the screened variety was significantly less than that of the control group. It provides a reliable reference for agronomic experts in terms of rape variety improvement and agricultural machinery experts regarding the optimization of rape harvesters.

Download Full-text