scholarly journals Inoculation with Different Nitrogen-Fixing Bacteria and Arbuscular Mycorrhiza Affects Grain Protein Content and Nodule Bacterial Communities of a Fava Bean Crop

Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 768
Author(s):  
Virginia Sánchez-Navarro ◽  
Raúl Zornoza ◽  
Ángel Faz ◽  
Catalina Egea-Gilabert ◽  
Margarita Ros ◽  
...  

The introduction of nitrogen fixing bacteria (NFB) and arbuscular mycorrhizal fungi (AMF) into the soil is an advisable agricultural practice for the crop, since it enhances nutrient and water uptake and tolerance to biotic and abiotic stresses. The aim of this work was to study plant nutrition, biological nitrogen fixation (BNF) and crop yield and quality, after inoculating seeds with NFBs ((Rhizobium leguminosarum, Burkholderia cenocepacia, Burkholderia vietnamiensis)) and/or AMFs (Rhizophagus irregularis, Claroideoglomus etunicatum, Claroideoglomus claroideum and Funneliformis mosseae) in a fava bean crop in two seasons. The composition of the nodule bacterial community was evaluated by the high-throughput sequencing analysis of bacterial 16 S rRNA genes. It was found that microbial inoculation accompanied by a 20% decrease in mineral fertilization had no significant effect on crop yield or the nutritional characteristics compared with a non-inoculated crop, except for an increase in the grain protein content in inoculated plants. None of the inoculation treatments increased biological nitrogen fixation over a non-inoculated level. The bacterial rRNA analysis demonstrated that the genus Rhizobium predominated in all nodules, both in inoculated and non-inoculated treatments, suggesting the previous presence of these bacteria in the soil. In our study, inoculation with Rhizobium leguminosarum was the most effective treatment for increasing protein content in seeds, while Burkholderia sp. was not able to colonise the plant nodules. Inoculation techniques used in fava beans can be considered an environmentally friendly alternative, reducing the input of fertilizers, while maintaining crop yield and quality, with the additional benefit of increasing the grain protein content. However, further research is required on the selection and detection of efficient rhizobial strains under local field conditions, above all those related to pH and soil type, in order to achieve superior nitrogen-fixing bacteria.

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1177
Author(s):  
Yuncai Hu ◽  
Gero Barmeier ◽  
Urs Schmidhalter

Cultivation of malting barley is particularly challenging as the requirements of growers, for high yield, and that of the brewing industry, seeking a specific quality criteria, must be met simultaneously. Furthermore, significant genotypic and environmental variations in grain yield and quality properties may occur. To investigate the relationships between grain yield and quality parameters of spring malting barley, a 2-year experiment was carried out in order to characterise the genotypic and year effects on grain yield, quality properties, and yield components of 23 high-yielding varieties of spring malting barley under optimal nitrogen (N) fertilisation. Compared to the grain quality properties of the grain protein content and the grain retention fraction of grain size >2.5 mm, less genotypic and environmental variation in grain yield was observed. Grain yield was closely related to spikes per m2, suggesting the importance of tiller formation and establishment as a decisive factor influencing malting barley yields. A major interactive effect of genotypes and year on grain size was observed. Regarding weather effects, the global radiation intensity during the post-anthesis phase was the major factor affecting the final grain size in this study. Grain protein content was primarily dependent on the year effect, suggesting that optimal N fertilisation levels must vary between years to ensure the correct protein content required for the needs of the brewing industry is met. Therefore, we recommend further development strategies addressing N fertilisation and soil N mineralisation to optimise the production of spring malting barley.


2012 ◽  
Vol 40 (4) ◽  
pp. 532-541 ◽  
Author(s):  
V. Mladenov ◽  
B. Banjac ◽  
A. Krishna ◽  
M. Milošević

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Saule Kenzhebayeva ◽  
Alfia Abekova ◽  
Saule Atabayeva ◽  
Gulzira Yernazarova ◽  
Nargul Omirbekova ◽  
...  

Deficiency of metals, primarily Fe and Zn, affects over half of the world’s population. Human diets dominated by cereal products cause micronutrient malnutrition, which is common in many developing countries where populations depend heavily on staple grain crops such as wheat, maize, and rice. Biofortification is one of the most effective approaches to alleviate malnutrition. Genetically stable mutant spring wheat lines (M7 generation) produced via 100 or 200 Gy gamma treatments to broaden genetic variation for grain nutrients were analyzed for nutritionally important minerals (Ca, Fe, and Zn), their bioavailability, and grain protein content (GPC). Variation was 172.3–883.0 mg/kg for Ca, 40.9–89.0 mg/kg for Fe, and 22.2–89.6 mg/kg for Zn. In mutant lines, among the investigated minerals, the highest increases in concentrations were observed in Fe, Zn, and Ca when compared to the parental cultivar Zhenis. Some mutant lines, mostly in the 100 Gy-derived germplasm, had more than two-fold higher Fe, Zn, and Ca concentrations, lower phytic acid concentration (1.4–2.1-fold), and 6.5–7% higher grain protein content compared to the parent. Variation was detected for the molar ratios of Ca:Phy, Phy:Fe, and Phy:Zn (1.27–10.41, 1.40–5.32, and 1.78–11.78, respectively). The results of this study show how genetic variation generated through radiation can be useful to achieve nutrient biofortification of crops to overcome human malnutrition.


Author(s):  
Isaiah O. Ochieng’ ◽  
Harun I. Gitari ◽  
Benson Mochoge ◽  
Esmaeil Rezaei-Chiyaneh ◽  
Joseph P. Gweyi-Onyango

2013 ◽  
Vol 13 (1) ◽  
pp. 35 ◽  
Author(s):  
Shengguan Cai ◽  
Gang Yu ◽  
Xianhong Chen ◽  
Yechang Huang ◽  
Xiaogang Jiang ◽  
...  

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e73751 ◽  
Author(s):  
Domenica Nigro ◽  
Yong Q. Gu ◽  
Naxin Huo ◽  
Ilaria Marcotuli ◽  
Antonio Blanco ◽  
...  

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 374
Author(s):  
Marta Aranguren ◽  
Ander Castellón ◽  
Ana Aizpurua

Adequate N fertilisation is crucial to increase the grain protein content (GPC) values in wheat. The recommended level of GPC needed to achieve high-quality bread-making flour should be higher than 12.5%. However, it is difficult to ensure the GPC values that the crop will achieve because N in grain is derived from two different sources: N remobilized into the grain from N accumulated in the pre-anthesis period, and N absorbed from the soil in the post-anthesis period. This study aimed to (i) evaluate the effect of the application of N on the rate of stem elongation (GS30) when farmyard manures are applied as initial fertilisers on GPC and on the chlorophyll meter (CM) values at mid-anthesis (GS65), (ii) establish a relationship between the CM values at GS65 and GPC, and (iii) determine a minimum CM value at GS65 to obtain GPC values above 12.5%. Three field trials were performed in three consecutive growing seasons, and different N fertilisation doses were applied. Readings using the CM Yara N-TesterTM were taken at GS65. The type of initial fertiliser did not affect the GPC and CM values. Generally, the greater the N application at GS30 is, the higher the GPC and CM values are. CM values can help to estimate GPC values only when yields are below 8000 kg ha−1. Additionally, CM values at GS65 should be higher than 700 to achieve high-quality bread-making flour (12.5%) at such yield levels. These results will allow farmers and cooperatives to make better decisions regarding late-nitrogen fertilisation and wheat sales.


2001 ◽  
Vol 29 (1-2) ◽  
pp. 33-40 ◽  
Author(s):  
R. K. Varshney ◽  
M. Prasad ◽  
J. K. Roy ◽  
M. S. Röder ◽  
H. S. Balyan ◽  
...  

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