scholarly journals S-Genotyping and Seed Paternity Testing of the Pear Cultivar ‘Celina’

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1372
Author(s):  
Fuad Gasi ◽  
Oddmund Frøynes ◽  
Belma Kalamujić Stroil ◽  
Lejla Lasić ◽  
Naris Pojskić ◽  
...  
Keyword(s):  
Fruit Set ◽  
Paternity Testing ◽  
Pear Cultivar ◽  
Fruit Cultivation ◽  
Pollen Donor ◽  
S Alleles ◽  
Green Background ◽  
Negative Effect ◽  
In The Beginning ◽  
Simple Sequence

The diploid Celina/QTee® (‘Colorée de Juillet’ × ‘Williams’), one of the most promising pear cultivars developed by the Norwegian breeding program Graminor, was launched in 2010. In Norway, the flowering is medium to late, while the fruits ripen in the beginning of September. The fruits are attractive with an intense red blush (50%) on a green background. Although, ‘Celina’ is cultivated in the most climatically suitable regions for fruit cultivation, present in Norway, unfavorable environmental conditions for pear pollination can have a very negative effect on fruit set and consequent yield. The aim of this study was to determine the S-alleles of ‘Celina’, as well as its frequently used pollinizers, and, through paternity testing of ‘Celina’ seeds, give a recommendation regarding the most important pollinizers of this pear cultivar. In order to accomplish this, ‘Celina’ and its potential pollinizers were all S-genotyped. After harvest, seeds collected from ‘Celina’ fruit in 2017 and 2018 were genotyped using eleven microsatellite markers. Genomic DNA was also extracted from leaf material collected from ‘Celina’, as well as from five pear cultivars used as pollinizers in the three examined orchards, and analyzed using the same marker set. Subsequently a simple sequence repeat (SSR) database was constructed and used for gene assignment analyses with the aim of quantifying pollen donor contribution from individual pollinizers. The obtained results indicate that ‘Anna’, the only examined pollinizer that was fully cross-compatible with ‘Celina’, together with ‘Fritjof’, the genotype which had the highest flowering overlap with ‘Celina’, proved to be the most successful pollinizers across all seasons and orchards. Although both cultivars were ubiquitous in the examined orchards, either as planted trees or as branches introduced during the flowering period, they were the most abundant pollinizers in only one orchard each. It is therefore possible to conclude that pollinizer abundance has a secondary significance in pollinizer success within investigated ‘Celina’ orchards.

scholarly journals Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2356
Author(s):  
Gabriela Vuletin Selak ◽  
Alenka Baruca Arbeiter ◽  
Julián Cuevas ◽  
Slavko Perica ◽  
Petar Pujic ◽  
...  
Keyword(s):  
Fruit Set ◽  
Single Case ◽  
Olive Tree ◽  
Fertilization Success ◽  
Paternity Analysis ◽  
Alternate Bearing ◽  
Pollen Donor ◽  
Cross Compatibility ◽  
Self Fertilization ◽  
Simple Sequence

The olive tree (Olea europaea L.) is a wind-pollinated crop that exhibits an extreme alternate bearing habit. To improve fruit set, several methods have been used to determine the most successful compatible combinations of cultivars. In this study, priority is given to seed paternity analysis based on simple sequence repeats (SSRs), microsatellite markers used for the identification of potential pollen donors of cultivar ‘Oblica’ in a mixed olive orchard during two consecutive years. Seven microsatellite primers were successfully used to examine the paternity of olive embryos from ‘Oblica’ mother trees. Embryos were considered as a product of self-fertilization if only maternal alleles were present, but not a single case of self-fertilization was found among all the embryos analyzed. Two dominant pollen donors were not the closest nor the cultivars with the highest number of trees in the orchard, suggesting that cross-compatibility may have a key role in determining pollen donor success. In our earlier studies, pollen tube growth and fertilization success correlated with fruit set when controlled crosses between cultivars were performed; however, some discrepancy might appear compared to paternity analyses when mother trees have a free choice among different pollen sources from cultivars growing in their surroundings.

scholarly journals Inheritance and linkage analysis of co-dominant SSR markers on the Z chromosome of the silkworm (Bombyx mori L.)

2008 ◽  
Vol 90 (2) ◽  
pp. 151-156 ◽  
Author(s):  
XUE-XIA MIAO ◽  
WEI-HUA LI ◽  
MU-WANG LI ◽  
YUN-PO ZHAO ◽  
XIAN-RU GUO ◽  
...  
Keyword(s):  
Linkage Map ◽  
Bombyx Mori ◽  
Ssr Markers ◽  
Positional Cloning ◽  
Z Chromosome ◽  
Pcr Marker ◽  
Backcross Population ◽  
In The Beginning ◽  
Simple Sequence ◽  
Visible Mutation

SummaryMicrosatellites or simple sequence repeats (SSRs) are co-dominant molecular markers. When we used fluorescent SSR markers to construct a linkage map for the female heterogametic silkworm (Bombyx mori, ZW), we found that some loci did not segregate in a Mendelian ratio of 1:1 in a backcross population. These loci segregated in a 3:1 ratio of single bands compared with double bands. Further examination of band patterns indicated that three types of SSR bands were present: two homozygotes and one heterozygote. In the beginning, we considered to discard these markers. By scoring male and female F1 individuals, we confirmed that these loci were located on the Z chromosome. Using the sex-linked visible mutation sch (K05) and its wild-type (C108), we constructed an F1 male backcross (BC1M) mapping population. The combination of sch backcross and SSR data enabled us to map the SSR markers to the Z chromosome. By adjusting input parameters based on these data, we were able to use Mapmaker software to construct a linkage map. This strategy takes advantage of co-dominant markers for positional cloning of genes on the Z chromosome. We localized sch to the Z chromosome relative to six SSR markers and one PCR marker, covering a total of 76·1 cM. The sch mutation is an important sex-linked visible mutation widely used in breeding of commercial silkworms (e.g. male silkworm selection rearing). Localization of the sch gene may prove helpful in cloning the gene and developing strains for marker-assisted selection in silkworm breeding.

scholarly journals Development of EST-derived SSR Markers with Long-core Repeat in Olive and Their Use for Paternity Testing

2013 ◽  
Vol 138 (4) ◽  
pp. 290-296 ◽  
Author(s):  
Raúl De la Rosa ◽  
Angjelina Belaj ◽  
Antonio Muñoz-Mérida ◽  
Oswaldo Trelles ◽  
Inmaculada Ortíz-Martín ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Simple Sequence Repeats ◽  
Expressed Sequence Tag ◽  
Paternity Testing ◽  
High Level ◽  
Repeated Motifs ◽  
Expressed Sequence ◽  
Simple Sequence ◽  
Olive Breeding ◽  
Genomic Project

In the present work, a set of eight new hexa-nucleotide simple sequence repeats (SSRs) is reported in olive (Olea europaea L). These SSRs loci were generated on the basis of expressed sequence tag (EST) sequences in the frame of an olive genomic project. The markers showed a high level of polymorphism when tested on a set of cultivars used as genitors in the olive breeding program of Córdoba, Spain. The long-core repeat motif of these markers allows a wider separation among alleles, thus permitting an accurate genotyping. Besides, these markers showed comparable levels of polymorphism to di-nucleotide SSRs, the only ones so far reported in olive. Selected on the basis of their discrimination capacity, four of the eight SSRs were used to test their ability for paternity testing in a total of 81 seedlings coming from 12 crosses. The paternity testing showed that seven crosses matched the alleged paternity and the remaining five were products of illicit pollinations. These results exactly matched with previous paternity testing performed with di-nucleotide SSR markers. These results demonstrate the usefulness of the developed hexa-nucleotide repeated motifs for checking the paternity of breeding progenies and suggest their use on variability studies.

FRUIT SET OF SWEET CHERRY 'GOMILICKA' IS INFLUENCED BY POLLEN DONOR GENOTYPE

2014 ◽  
pp. 65-69
Author(s):  
M. Radunić ◽  
S. Goreta Ban ◽  
G. Vuletin Selak ◽  
A. Jazbec ◽  
Z. Čmelik
Keyword(s):  
Sweet Cherry ◽  
Fruit Set ◽  
Pollen Donor ◽  
Donor Genotype

Beetle pollination and fruit predation of Xanthosoma daguense (Araceae) in an Andean cloud forest in Colombia

2004 ◽  
Vol 20 (4) ◽  
pp. 459-469 ◽  
Author(s):  
Carlos García-Robledo ◽  
Gustavo Kattan ◽  
Carolina Murcia ◽  
Paulina Quintero-Marín
Keyword(s):  
Reproductive Success ◽  
Positive Relationship ◽  
Fruit Set ◽  
Cloud Forest ◽  
Female Reproductive Success ◽  
Hand Pollination ◽  
Negative Effect ◽  
Tropical Cloud Forest ◽  
Nitidulid Beetles ◽  
Pollination Systems

This study describes a pollination system in a species of Araceae that involves three species of beetle, one of which is also a fruit predator. In a tropical cloud forest in Colombia, inflorescences of Xanthosoma daguense opened at dusk, releasing a sweet scent and raising their temperature 1–3 °C. Soon after, two species of Scarabaeidae (Dynastinae; Cyclocephala gregaria and C. amblyopsis) and one species of Nitidulidae (Macrostola costulata) arrived with pollen. Cyclocephala beetles remained inside the inflorescence for 24 h. The next night, Cyclocephala beetles left the inflorescence after picking up the freshly shed pollen, almost always moving to the nearest inflorescence available. The probability of inflorescence abortion and number of fruits set after the visit of one individual was equivalent for both Cyclocephala species. However, C. gregaria was much more abundant than C. amblyopsis, so it was the most important pollinator. There was a positive relationship between the number of dynastine visits and the number of fruits produced. Besides carrying pollen to the inflorescences, nitidulid beetles had a negative effect on female reproductive success through fruit predation. Nitidulid larvae developed inside the infructescence and preyed on up to 64% of the fruits. However, 8% of inflorescences not visited by dynastines were probably pollinated by nitidulids, because hand-pollination experiments showed that self-pollination was unlikely. Inflorescences potentially pollinated by nitidulids comprised 25% of the fruit crop in the year of our study. This interaction with a fruit predator that is also a potential pollinator resembles brood-site pollination systems in which pollinators prey on part of the fruit set (e.g. Ficus, senita cacti, Yucca), making this system substantially more complex than previously described dynastine-pollinated systems in aroids.

scholarly journals Cross-compatibility of five highbush blueberry varieties and ideal crossing combinations

2019 ◽  
Author(s):  
Keanu Martin ◽  
Corneile Minnaar ◽  
Marinus de Jager ◽  
Bruce Anderson
Keyword(s):  
South Africa ◽  
Fruit Set ◽  
Developmental Time ◽  
Highbush Blueberry ◽  
Agricultural Crops ◽  
Compatibility Studies ◽  
Negative Effects ◽  
Pollen Donor ◽  
Cross Compatibility ◽  
Fruit Mass

AbstractBlueberry plants require large quantities of pollen deposited on stigmas to produce commercial-quality fruit. Like many agricultural crops, the interaction between pollen-source variety and pollen-recipient variety can be a major determinant of fruit quality in blueberries. However, little information exists to guide growers in optimising fruit set and quality. Using five commonly grown blueberry varieties, I determined whether crossing between varieties (inter-varietal) increased fruit mass and decreased developmental time relative to crossing within a variety (intra-varietal), and if so, what the best crossing combinations are. While intra-varietal pollination often produced fruit, for certain varieties the fruit set and fruit mass were highly reduced compared to inter-varietal pollination. Furthermore, intra-varietal pollination resulted in longer fruit developmental time in comparison to pollination between varieties. For the same pollen-recipient variety, inter-varietal crosses typically outperformed intra-varietal crosses in fruit mass and developmental time; however, the extent to which inter-varietal crosses outperformed intra-varietal crosses differed between pollen-donor varieties. This result suggests that combinations of varieties are not trivial as some inter-varietal combinations may outperform others. Furthermore, some varieties appear to be more susceptible to the negative effects of intra-varietal crosses than others and that less susceptible varieties may be better suited to conditions where pollinator movement is poor. While our study can guide growers in determining optimal co-planting schemes for the varieties tested, for example in South Africa where these varieties are frequently grown. It also serves as a blueprint for similar compatibility studies that can easily be performed prior to planting to determine the best inter-varietal combinations.

scholarly journals Severely pollen-limited fruit set in a pear (Pyrus communis) orchard revealed by yield assessments and DNA-based paternity assignment of seedlings

2007 ◽  
Vol 13 (3) ◽  
Author(s):  
H. Mattison ◽  
J. Sehic ◽  
H. Nybom
Keyword(s):  
Fruit Set ◽  
Seed Set ◽  
Rapd Analysis ◽  
Total Yield ◽  
Fruit Tree ◽  
Pollen Transfer ◽  
Pear Cultivar ◽  
Pollination Success ◽  
Viable Seeds ◽  
High Yields

In commercial fruit tree orchards, consistently high yields are necessary for a durable economy. The Swedish pear cultivar 'Carola' has been noted for low setting in some orchards, possibly due to insufficient pollination. In this study, fruit set was evaluated in a research orchard where `Carola' had been planted together with four potential pollinators. Total yield and number of fruits was noted during three and four years, respectively. In 2003, seeds were germinated from the harvested `Carola' fruits, and the paternity of three seedlings from 50 trees was determined with RAPD analysis. 'Clapp's Favourite' had sired 39.6% of the seedlings, closely followed by `Seigneur d'Esperen' (30.7%) and 'Clara Frijs' (26.7%) whereas 'Skanskt sockerparon' only sired 1.1% of the seedlings. The remaining 2.3% appeared to have been derived by selfing. Pollen-limited seed set was indicated at surprisingly short distances; accumulated number of fruits on the `Carola' trees was significantly higher when separated by only 2 m from one of the two most efficient pollinators, 'Clapp's Favourite' or 'Seigneur d'Esperen‘, compared to trees 4—l0 in away in the same row. Number of viable seeds per fruit was also higher in fruits from trees immediately adjacent to the pollinators, suggesting an effect of improved pollination success. The importance of very short inter-cultivar distances for efficient pollen transfer became even more clear when comparisons involved the true pollination distances as determined by RAPD; the accumulated yields decreased linearly from 55 kg at a 2 in distance to only 17 kg at 13 m.

scholarly journals Pollination of ‘Hortblue Petite’ Blueberry: Evidence of Metaxenia in a New Ornamental Home-garden Cultivar

HortScience ◽  
2011 ◽  
Vol 46 (11) ◽  
pp. 1468-1471 ◽  
Author(s):  
Shirley Miller ◽  
Peter Alspach ◽  
Jessica Scalzo ◽  
John Meekings
Keyword(s):  
New Zealand ◽  
Fruit Set ◽  
Vaccinium Corymbosum ◽  
Fruit Weight ◽  
Yield Potential ◽  
Home Garden ◽  
Retail Sector ◽  
Berry Weight ◽  
Pollen Donor ◽  
Viable Seeds

‘Hortblue Petite’ (Vaccinium corymbosum L.) is a compact, small-statured tetraploid blueberry that was introduced to the New Zealand retail sector as a garden ornamental in 2005. Pollination requirements for ‘Hortblue Petite’ have not previously been investigated and this study was undertaken to better understand compatibility with other blueberry cultivars. Pollen donor effects on fruit weight were significant; pollen from large fruiting cultivars produced bigger fruit in ‘Hortblue Petite’, adding support toward metaxenia occurring in blueberry. Outcrossing with other tetraploids was most successful in terms of fruit set, berry weight, and yield potential. Number of viable seeds per berry was less with self-pollination and with crosses using pollen from a hexaploid rabbiteye type. The data indicated that the best yields would be obtained from using either open-pollinated flowers or a large-fruited cultivar such as ‘Nui’.

Bitcoin

2015 ◽  
Vol 6 (2) ◽  
pp. 52-71 ◽  
Author(s):  
Tetsuya Saito
Keyword(s):  
Inflation Rate ◽  
Substitution Effect ◽  
Large Share ◽  
Public Authorities ◽  
The Public ◽  
Matching Process ◽  
Negative Effect ◽  
Money Search ◽  
The Stability ◽  
In The Beginning

This paper considers whether the stability of Bitcoin in the market as a method of payment using a dual currency money-search model. In the model, there is traditional money and Bitcoin. The two currencies are classified by the storage cost and the probability that sellers accept particular money for payments. Agents are randomly matched for transactions. To consider substitution effect between monies, we allow new entries every period. In the beginning of each period, new entrants come into the matching process with a unit of money of their choice. A certain number of sellers also come into the same process to maintain the population share of sellers at a constant level. With appropriately chosen parameters, the author finds that there can be stable and unstable equilibria of the share of bitcoiners. In this case, a stable equilibrium is a success (bitcoiners take a large share) while the other (unstable) is a failure (bitcoiners take a marginal share or vanish). However, if the inflation rate of traditional money decreases, the successful equilibrium disappears to start approaching the failure even if Bitcoin is currently widely accepted. Furthermore, welfare comparisons suggest that an increase in the share of bitcoiners has a negative effect; hence, the benefit from reductions in the transaction costs must compensate for the welfare erosion if Bitcoin is accepted as a new kind of payment system. If the author is to succeed, the Bitcoin community or the public authorities need to be prepared for protecting the system from several illicit activities.

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