scholarly journals Recovery from Grapevine Flavescence Dorée in Areas of High Infection Pressure

Agronomy ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1479
Author(s):  
Matteo Ripamonti ◽  
Davide Pacifico ◽  
Chiara Roggia ◽  
Sabrina Palmano ◽  
Marika Rossi ◽  
...  
Keyword(s):  
Management Strategies ◽  
Spontaneous Remission ◽  
Wine Quality ◽  
Wine Production ◽  
Flavescence Dorée ◽  
Infection Pressure ◽  
Scaphoideus Titanus ◽  
High Infection ◽  
Sensory Analyses ◽  
Grapevine Disease

Flavescence dorée (FD) is a quarantine grapevine disease caused by a phytoplasma transmitted by the leafhopper Scaphoideus titanus Ball. FD management relies on compulsory insecticide treatments, roguing of infected plants, and substitution with certified material. Some grapevine cultivars show a spontaneous remission of symptoms (recovery). To determine if recovery is a suitable strategy to co-exist with disease in areas of strong infestation, the qualitative aspects of grapes, musts, and wines obtained from recovered Barbera and Chardonnay grapevines were investigated in two productive vineyards. Following field observations, about 1500 plants in each vineyard were divided into healthy (asymptomatic and negative in phytoplasma molecular diagnosis) and recovered (asymptomatic the year of observation but infected the year before). Maturation curves and microvinification tests followed by oenological and sensory analyses showed that maturation trends of recovered grapes were in line with those from healthy plants and the final qualities of wines were comparable. The spread of FD has strongly increased in Piedmont (Italy) in recent decades. Management strategies to cope with the disease are necessary to preserve traditional wine production. Despite the yield from recovered grapevines is quantitatively lower than that from healthy ones, we showed here that the wine quality is, however, preserved.

scholarly journals Occurrence of Scaphoideus titanus Ball and some other Auchenorrhyncha in the vineyards of western Slovakia

2016 ◽  
Vol 53 (No. 2) ◽  
pp. 96-100
Author(s):  
Tancik Ján ◽  
Seljak Gabriel
Keyword(s):  
Management Strategies ◽  
National Strategy ◽  
Bois Noir ◽  
Potential Vector ◽  
Flavescence Dorée ◽  
First Finding ◽  
Scaphoideus Titanus ◽  
Phytoplasma Vector ◽  
Wine Region ◽  
And Control

A study of Auchenorrhyncha was carried out in 2014 and 2015 in 7 vineyard plots with different varieties and pest management strategies in the Nitra wine region and Lesser Carpathian wine region in western Slovakia. The aim of this study was to obtain information related to the presence of potential vector insects associated with grapevine yellows phytoplasmas from the Flavescence dorée and Bois noir groups. Insects were collected by sweeping with an entomological net. Thirty species of Auchenorrhyncha were identified as belonging to 6 families. Cicadellidae were the most abundant, comprising 20 species. Scaphoideus titanus was collected at 4 localities. Identification of the phytoplasma vector is critical to the national strategy for assessment and control of vectors spreading the phytoplasma disease in Slovakian vineyards. The first finding of Metcalfa pruinosa was noticed in vineyards in Slovakia.

scholarly journals New Viral Sequences Identified in the Flavescence Dorée Phytoplasma Vector Scaphoideus titanus

Viruses ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 287
Author(s):  
Sara Ottati ◽  
Marco Chiapello ◽  
Luciana Galetto ◽  
Domenico Bosco ◽  
Cristina Marzachì ◽  
...  
Keyword(s):  
Large Scale ◽  
Control Measures ◽  
Insect Vector ◽  
Wine Production ◽  
Flavescence Dorée ◽  
Reverse Transcription Pcr ◽  
Scaphoideus Titanus ◽  
The Usa ◽  
In The Wild ◽  
Increasing Demand

(1) Background: The leafhopper Scaphoideus titanus is the primary vector of Flavescence dorée phytoplasma (FDp) in European vineyards. Flavescence dorée is one of the most severely damaging diseases of Vitis vinifera and, consequently, a major threat to grape and wine production in several European countries. Control measures are compulsory, but they mainly involve large-scale insecticide treatments, with detrimental impacts on the environment. One possible solution is to exploit the largely unexplored genetic diversity of viruses infecting S. titanus as highly specific and environmentally benign tools for biological control. (2) Methods: A metatranscriptomic approach was adopted to identify viruses that may infect individuals caught in the wild in both its native (United States) and invasive (Europe) areas. Reverse transcription PCR was used to confirm their presence in RNA pools and explore their prevalence. (3) Results: We described nine new RNA viruses, including members of “Picorna-Calici”, “Permutotetra”, “Bunya-Arena”, “Reo”, “Partiti-Picobirna”, “Luteo-Sobemo” and “Toti-Chryso” clades. A marked difference in the diversity and abundance of the viral species was observed between the USA population and the European ones. (4) Conclusions: This work represents the first survey to assess the viral community of a phytoplasma insect vector. The possibility to exploit these naturally occurring viruses as specific and targeted biocontrol agents of S. titanus could be the answer to increasing demand for a more sustainable viticulture.

scholarly journals Acquisition capability of the grapevine Flavescence dorée by the leafhopper vector Scaphoideus titanus Ball correlates with phytoplasma titre in the source plant

2014 ◽  
Vol 87 (4) ◽  
pp. 671-679 ◽  
Author(s):  
Luciana Galetto ◽  
Dimitrios Miliordos ◽  
Chiara Roggia ◽  
Mahnaz Rashidi ◽  
Dario Sacco ◽  
...  
Keyword(s):  
Leafhopper Vector ◽  
Flavescence Dorée ◽  
Scaphoideus Titanus ◽  
Source Plant

scholarly journals Models Applied to Grapevine Pests: A Review

Insects ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 169
Author(s):  
Federico Lessio ◽  
Alberto Alma
Keyword(s):  
Lobesia Botrana ◽  
Grape Berry ◽  
Homalodisca Vitripennis ◽  
Flavescence Dorée ◽  
Or Groups ◽  
Scaphoideus Titanus ◽  
Different Types ◽  
Computer Based ◽  
Biological Aspects ◽  
Definition Of

This paper reviews the existing predictive models concerning insects and mites harmful to grapevine. A brief conceptual description is given on the definition of a model and about different types of models: deterministic vs. stochastics, continuous vs. discrete, analytical vs. computer-based, and descriptive vs. data-driven. The main biological aspects of grapevine pests covered by different types of models are phenology, population growth and dynamics, species distribution, and invasion risk. A particular emphasis is put on forecasting epidemics of plant disease agents transmitted by insects with sucking-piercing mouthparts. The most investigated species or groups are the glassy-winged sharpshooter Homalodisca vitripennis (Germar) and other vectors of Xylella fastidiosa subsp. fastidiosa, a bacterium agent of Pierce’s disease; the European grape berry moth, Lobesia botrana (Denis and Schiffermuller); and the leafhopper Scaphoideus titanus Ball, the main vector of phytoplasmas agents of Flavescence dorée. Finally, the present and future of decision-support systems (DSS) in viticulture is discussed.

scholarly journals How can remote sensing techniques help monitoring the vine and maximize the terroir potential?

2018 ◽  
Vol 50 ◽  
pp. 02007
Author(s):  
Cecile Tondriaux ◽  
Anne Costard ◽  
Corinne Bertin ◽  
Sylvie Duthoit ◽  
Jérôme Hourdel ◽  
...  
Keyword(s):  
Remote Sensing ◽  
High Resolution ◽  
Satellite Images ◽  
Sampling Strategy ◽  
Wine Quality ◽  
Wine Production ◽  
Spectral Bands ◽  
High Resolution Satellite Images ◽  
Remote Sensing Techniques ◽  
Set Up

In each winegrowing region, the winegrower tries to value its terroir and the oenologists do their best to produce the best wine. Thanks to new remote sensing techniques, it is possible to implement a segmentation of the vineyard according to the qualitative potential of the vine stocks and make the most of each terroir to improve wine quality. High resolution satellite images are processed in several spectral bands and algorithms set-up specifically for the Oenoview service allow to estimate vine vigour and a heterogeneity index that, used together, directly reflect the vineyard oenological potential. This service is used in different terroirs in France (Burgundy, Languedoc, Bordeaux, Anjou) and in other countries (Chile, Spain, Hungary and China). From this experience, we will show how remote sensing can help managing vine and wine production in all covered terroirs. Depending on the winegrowing region and its specificities, its use and results present some differences and similarities that we will highlight. We will give an overview of the method used, the advantage of implementing field intra-or inter-selection and how to optimize the use of amendment and sampling strategy as well as how to anticipate the whole vineyard management.

scholarly journals Acquisition of Flavescence Dorée Phytoplasma by Scaphoideus titanus Ball from Different Grapevine Varieties

2016 ◽  
Vol 17 (9) ◽  
pp. 1563 ◽  
Author(s):  
Luciana Galetto ◽  
Dimitrios Miliordos ◽  
Mattia Pegoraro ◽  
Dario Sacco ◽  
Flavio Veratti ◽  
...  
Keyword(s):  
Flavescence Dorée ◽  
Scaphoideus Titanus

scholarly journals First Report of Flavescence Dorée-Related Phytoplasma Affecting Grapevines in Croatia

Plant Disease ◽  
2011 ◽  
Vol 95 (3) ◽  
pp. 353-353 ◽  
Author(s):  
M. Šeruga Musić ◽  
D. Škorić ◽  
I. Haluška ◽  
I. Križanac ◽  
J. Plavec ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Reference Strain ◽  
Simultaneous Detection ◽  
Pinot Noir ◽  
Vitis Vinifera L ◽  
Rrna Gene ◽  
Pcr Assay ◽  
Direct Pcr ◽  
Flavescence Dorée ◽  
Scaphoideus Titanus

Flavescence dorée (FD) and Bois noir (BN) phytoplasmas are principal grapevine yellows (GY) agents in the wider Euro-Mediterranean Region. While BN phytoplasma belongs to the ribosomal subgroup 16SrXII-A, the FD agents belong either to the ribosomal subgroups 16SrV-C or -D. During the official GY survey in 2009, 40 symptomatic grapevines (Vitis vinifera L.) were sampled throughout grapevine-growing regions in Croatia. Typical GY symptoms of leaf yellowing or reddening were evident on white and red varieties, respectively. Leaf rolling as well as irregular lignification of the shoots and withering of clusters were also observed. Phloem tissue from cuttings and leaf veins from mature vines were sampled for total DNA extraction and amplification of phytoplasma 16S rRNA gene by using generic primers P1/P7 in a direct PCR assay followed by a nested PCR using primer pair R16F2n/R2 (2). Phytoplasma ribosomal group affiliation was determined by restriction fragment length polymorphism (RFLP) analysis of the nested PCR products with enzyme Tru1I (Fermentas, Vilnius, Lithuania). These initial findings were validated and augmented by a triplex real-time PCR assay targeting the nonribosomal map gene. This assay enables simultaneous detection of BN and FD (16SrV-C and -D) phytoplasmas in grapevine (3). Assay results revealed the majority of GY positive vines (19 of 40) contained BN phytoplasma which is widespread. For the first time in Croatia, two red variety samples, Pinot Noir and Plemenka Crvena, from the vicinity of Ozalj (Vivodina) and Zagreb (Brezje), respectively, were found to harbor FD-related phytoplasmas. Fragments amplified by P1/P7 primers from latter samples were cloned and sequenced. Sequence analyses using online interactive tool iPhyClassifier (4) revealed that the phytoplasma under study from Pinot Noir sample (GenBank Accession No. HQ712064) is a member of 16SrV-C subgroup and shares 99.87% similarity with 16S rDNA sequence of the reference strain (GenBank Accession No. AF176319). The sequence from the Plemenka Crvena sample (GenBank Accession No. HQ712065) shares 99.54% similarity with the reference strain and has the most similar virtual RFLP pattern to the one of the 16SrV-C subgroup (GenBank Accession No. AY197642). These findings are currently limited to vineyards in northwestern Croatia. Even so, the presence of FD principal cicadellid vector Scaphoideus titanus in the country and the occurrence and distribution of FD in neighboring countries (1,2) are factors indicating that the spread of FD in Croatia is highly probable. References: (1) L. Filippin et al. Plant Pathol. 58:826, 2009. (2) S. Kuzmanović et al. Vitis 47:105, 2008. (3) C. Pelletier et al. Vitis 48:87, 2009. (4) Y. Zhao et al. Int. J. Syst. Evol. Microbiol. 59:2582, 2009.

scholarly journals Silencing of ATP Synthase β Impairs Egg Development in the Leafhopper Scaphoideus titanus, Vector of the Phytoplasma Associated with Grapevine Flavescence Dorée

2022 ◽  
Vol 23 (2) ◽  
pp. 765
Author(s):  
Matteo Ripamonti ◽  
Luca Cerone ◽  
Simona Abbà ◽  
Marika Rossi ◽  
Sara Ottati ◽  
...  
Keyword(s):  
Atp Synthase ◽  
Cathepsin L ◽  
Field Application ◽  
Female Sterility ◽  
Model Species ◽  
Egg Development ◽  
Flavescence Dorée ◽  
Vector Population ◽  
Regulatory Constraints ◽  
Scaphoideus Titanus

Scaphoideus titanus (Hemiptera: Cicadellidae) is the natural vector of Flavescence dorée phytoplasma, a quarantine pest of grapevine with severe impact on European viticulture. RNA interference (RNAi) machinery components are present in S. titanus transcriptome and injection of ATP synthase β dsRNAs into adults caused gene silencing, starting three days post injection (dpi) up to 20 dpi, leading to decrease cognate protein. Silencing of this gene in the closely related leafhopper Euscelidiusvariegatus previously showed female sterility and lack of mature eggs in ovaries. Here, alteration of developing egg morphology in S. titanus ovaries as well as overexpression of hexamerin transcript (amino acid storage protein) and cathepsin L protein (lysosome proteinase) were observed in dsATP-injected females. To evaluate RNAi-specificity, E.variegatus was used as dsRNA-receiving model-species. Different doses of two sets of dsRNA-constructs targeting distinct portions of ATP synthase β gene of both species induced silencing, lack of egg development, and female sterility in E. variegatus, indicating that off-target effects must be evaluated case by case. The effectiveness of RNAi in S. titanus provides a powerful tool for functional genomics of this non-model species and paves the way toward RNAi-based strategies to limit vector population, despite several technical and regulatory constraints that still need to be overcome to allow open field application.

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