scholarly journals Exogenous Auxin-Mediated Salt Stress Alleviation in Faba Bean (Vicia faba L.)

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 547
Author(s):  
Arafat Abdel Hamed Abdel Latef ◽  
Md. Tahjib-Ul-Arif ◽  
Mohammad Saidur Rhaman
Keyword(s):  
Salt Stress ◽  
Vicia Faba ◽  
Faba Bean ◽  
Stress Responses ◽  
Foliar Application ◽  
Soluble Sugar ◽  
Principal Component ◽  
Vicia Faba L ◽  
Catalase Peroxidase ◽  
Amino Acid Contents

Auxin not only controls the development processes, but also regulates the stress responses of plants. In this investigation, we explored the potential roles of exogenously applied indole-3-acetic acid (IAA) in conferring salt tolerance in the faba bean (Vicia faba L.). Our results showed that foliar application of IAA (200 ppm) to salt-exposed (60 mM and 150 mM NaCl) plants promoted growth, which was evidenced by enhanced root–stem traits. IAA application ensured better osmotic protection in salt-stressed plants which was supported by reduced proline and enhanced soluble sugar, soluble protein, and total free amino acid contents in the roots, stem, and seeds. IAA application also increased the number of nodules in salt-stressed plants, which may facilitate better nitrogen assimilation. Moreover, IAA mediated improvements in mineral homeostasis (K+, Ca2+, and Mg2+) and the translocation of Na+, while it also inhibited excessive accumulation of Na+ in the roots. Salt-induced oxidative damage resulted in increased accumulation of malondialdehyde, whereas IAA spraying relegated malondialdehyde by improving antioxidant enzymes, including superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase. Together, these results together with a principal component analysis uncovered that foliar spraying of IAA alleviated the antagonistic effects of salt stress via enhancing osmolyte accumulation, ionic homeostasis, and antioxidant activity. Finally, exogenous IAA enhanced the yield of broad beans under high salinity conditions.

scholarly journals Foliar Application of Auxin or Cytokinin Can Confer Salinity Stress Tolerance in Vicia faba L.

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 790
Author(s):  
Arafat Abdel Hamed Abdel Latef ◽  
Ayasha Akter ◽  
Md. Tahjib-Ul-Arif
Keyword(s):  
Vicia Faba ◽  
Soil Salinity ◽  
Faba Bean ◽  
Foliar Application ◽  
Growth Traits ◽  
Soluble Sugars ◽  
Principal Component ◽  
Lower Growth ◽  
Significant Difference ◽  
Vicia Faba L

Soil salinity severely declines the availability of water and essential minerals to the plants, which hinders growth. The present study evaluates the potential roles of indole-3-acetic acid (IAA) and 6-benzyladenine (BA) for mitigating the adverse effects of soil-salinity in faba bean (Vicia faba L.). Plants were exposed to 150 mM NaCl stress and were sprayed with IAA (1.15 mM) or BA (0.9 mM). Our results revealed that foliar application of IAA or BA improved the growth traits of salinized faba bean due to the increased uptake of K+, Ca2+, and Mg2+ ions, accumulation of free amino acids, soluble sugars, and soluble proteins, and activity of superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase. The principal component analysis (PCA) and heatmap clustering indicated that salinity-exposed plants exhibited lower growth and biomass production, which correlated with higher accumulation of Na+ and malondialdehyde. Moreover, electrophoretic patterns of protein showed new bands in IAA- or BA-treated salt-stressed plants, indicating that IAA or BA treatment can reprogram the metabolic processes to confer salinity tolerance. We also found that IAA has a greater capacity to ameliorate the salt stress than BA, although there is no significant difference in yield between these treatments. Finally, these findings can be helpful for a better understanding of IAA- and BA-mediated salt tolerance mechanisms and increasing production of faba bean in saline soils.

scholarly journals Enhanced Abiotic Stress Tolerance of Vicia faba L. Plants Heterologously Expressing the PR10a Gene from Potato

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 173
Author(s):  
Abeer F. Desouky ◽  
Ahmed H. Ahmed ◽  
Hartmut Stützel ◽  
Hans-Jörg Jacobsen ◽  
Yi-Chen Pao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Vicia Faba ◽  
Faba Bean ◽  
Abiotic Stress Tolerance ◽  
Wild Type ◽  
Stress Injury ◽  
Bean Plants ◽  
Vicia Faba L

Pathogenesis-related (PR) proteins are known to play relevant roles in plant defense against biotic and abiotic stresses. In the present study, we characterize the response of transgenic faba bean (Vicia faba L.) plants encoding a PR10a gene from potato (Solanum tuberosum L.) to salinity and drought. The transgene was under the mannopine synthetase (pMAS) promoter. PR10a-overexpressing faba bean plants showed better growth than the wild-type plants after 14 days of drought stress and 30 days of salt stress under hydroponic growth conditions. After removing the stress, the PR10a-plants returned to a normal state, while the wild-type plants could not be restored. Most importantly, there was no phenotypic difference between transgenic and non-transgenic faba bean plants under well-watered conditions. Evaluation of physiological parameters during salt stress showed lower Na+-content in the leaves of the transgenic plants, which would reduce the toxic effect. In addition, PR10a-plants were able to maintain vegetative growth and experienced fewer photosystem changes under both stresses and a lower level of osmotic stress injury under salt stress compared to wild-type plants. Taken together, our findings suggest that the PR10a gene from potato plays an important role in abiotic stress tolerance, probably by activation of stress-related physiological processes.

scholarly journals Agro-Morphological and Molecular Variability among Algerian Faba Bean (Vicia faba L.) Accessions

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1456
Author(s):  
Nawel Malek ◽  
Meriem Miyassa Aci ◽  
Khalil Khamassi ◽  
Antonio Lupini ◽  
Mustapha Rouaissi ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Morphological Traits ◽  
Crop Improvement ◽  
Principal Component ◽  
Molecular Data ◽  
Mantel Test ◽  
Ssr Analysis ◽  
Fruit Setting ◽  
Vicia Faba L

Faba bean (Vicia faba L.) Algerian accessions represent an essential source of traits of interest for crop improvement, especially for tackling climate change, because their genetic background and potential have not been well studied. The purpose of this research was to assess the genetic variability of 14 Algerian faba bean accessions by means of 10 agro-morphological traits and 7 simple sequence repeat markers (SSRs). ANOVA analysis showed a large significant phenotypic variation in fruit setting (FS), seed length (SL), seed width (SW), and 100-seeds weight (HSW), which arose as the main discriminant characters as revealed by principal component analysis (PCA). In addition, SSR analysis identified a total of twenty different alleles within our collection with a mean of 2.85 alleles per locus. The polymorphism information content (PIC) ranged from 0.32 to 0.58, with a mean of 0.44. Observed heterozygosity (Ho) ranged from 0.57 to 1.00 with a mean of 0.72, while the expected one (He) varied from 0.42 to 0.67, reaching a mean of 0.57. Based on agro-morphological as well as molecular data, the 14 accessions were not clustered according to the geographical pattern, as also confirmed by principal coordinate analysis (PCoA). Moreover, AMOVA highlighted that most of the overall genetic variation within our collection was the result of strong differentiation among accessions (84%). Finally, the Mantel test revealed that there was no substantial correlation between the molecular and agro-morphological traits (r = −0.025, p > 0.05). These findings represent a first step toward faba been breeding programs establishment in Algeria, indicating that our collection exhibited optimal agro- and molecular diversity to identify specific traits useful in Mediterranean environment.

Influence of host genotypes on growth, symbiotic performance and nitrogen assimilation in faba bean (Vicia faba L.) under salt stress

1995 ◽  
Vol 172 (2) ◽  
pp. 289-297 ◽  
Author(s):  
Maria del Pilar Cordovilla ◽  
Francisco Ligero ◽  
Carmen Lluch
Keyword(s):  
Salt Stress ◽  
Vicia Faba ◽  
Faba Bean ◽  
Nitrogen Assimilation ◽  
Vicia Faba L
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