scholarly journals Poriella subacida Gen. & Comb Nov. for Perenniporia subacida (Peck) Donk

Agronomy ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1308
Author(s):  
Rui Chen ◽  
Samantha C. Karunarathna ◽  
Chang-Lin Zhao
Keyword(s):  
Elongation Factor ◽  
Type Specimen ◽  
Small Subunit ◽  
White Rot ◽  
Its2 Region ◽  
Gene Encoding ◽  
Translation Elongation ◽  
Bayesian Inferences ◽  
Elongation Factor 1 ◽  
Mitochondrial Rdna

Poriella subacida gen. & comb. nov., previously known as Perenniporia subacida, which causes white rot, has been documented in temperate and tropical forests. Specimens from Asia, North America, and Europe were examined, including the type specimen of Polylorus subacidus. Sequences of the ITS1-5.8S-ITS2 region, the 28S rDNA, the mitochondrial rDNA small subunit (mtSSU), and the gene encoding the translation elongation factor 1-α (EF1) were generated. In multigene phylogenies (maximum parsimony, maximum likelihood, Bayesian inferences), “Perenniporia subacida” formed a well-supported lineage, distinct from the core “Perenniporia” clade (type species: “P. medulla-panis”), and sister to the “Yuchengia narymica” lineage. We therefore conclude that “P. subacida” should be placed in the new genus “Poriella”gen. nov. Morphologically, “Poriella” is characterized by a di- to trimitic hyphal system, non-truncate basidiospores, and strongly dextrinoid, cyanophilic skeletal hyphae.

scholarly journals Identification and diversity of Fusarium species isolated from tomato fruits

2016 ◽  
Vol 56 (3) ◽  
pp. 231-236 ◽  
Author(s):  
Nur Baiti Abd Murad ◽  
Nor Azizah Kusai ◽  
Nur Ain Izzati Mohd Zainudin
Keyword(s):  
Fusarium Species ◽  
Elongation Factor ◽  
Fungal Species ◽  
Fruit Rot ◽  
Gene Encoding ◽  
Translation Elongation ◽  
Additional Information ◽  
Rot Disease ◽  
Serious Disease ◽  
Elongation Factor 1

Abstract Fruit rot of tomato is a serious disease caused by Fusarium species. Sampling was conducted throughout Selangor, Malaysia and fungal species identification was conducted based on morphological and gene encoding translation elongation factor 1-α (tef1-α) sequence analysis. Five species of Fusarium were discovered namely F. oxysporum (including F. oxysporum f. sp. lycopersici), F. solani, F. equiseti, F. proliferatum and F. verticillioides. Our results provide additional information regarding the diversity of Fusarium species associated with fruit rot disease of tomato.

scholarly journals Increased expression of Saccharomyces cerevisiae translation elongation factor 1 alpha bypasses the lethality of a TEF5 null allele encoding elongation factor 1 beta.

Genetics ◽  
1995 ◽  
Vol 141 (2) ◽  
pp. 481-489 ◽  
Author(s):  
T G Kinzy ◽  
J L Woolford
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Null Allele ◽  
Elongation Factor ◽  
Wild Type ◽  
Translation Elongation Factor ◽  
Gene Encoding ◽  
Translation Elongation ◽  
Dominant Mutant ◽  
Cold Sensitive ◽  
Elongation Factor 1

Abstract Translation elongation factor 1beta (EF-1beta) catalyzes the exchange of bound GDP for GTP on EF-1alpha. The lethality of a null allele of the TEF5 gene encoding EF-1beta in Saccharomyces cerevisiae was suppressed by extra copies of the TEF2 gene encoding EF-1alpha. The strains with tef5::TRP1 suppressed by extra copies of TEF were slow growing, cold sensitive, hypersensitive to inhibitors of translation elongation and showed increased phenotypic suppression of +1 frameshift and UAG nonsense mutations. Nine dominant mutant alleles of TEF2 that cause increased suppression of frameshift mutations also suppressed the lethality of tef5::TRP1. Most of the strains in which tef5::TRP1 is suppressed by dominant mutant alleles of TEF2 grew more slowly and were more antibiotic sensitive than strains with tef5::TRP1 is suppressed by wild-type TEF2. Two alleles, TEF2-4 and TEF2-10, interact with tef5::TRP1 to produce strains that showed doubling times similar to tef5::TRP1 strains containing extra copies of wild-type TEF2. These strains were less cold sensitive, drug sensitive and correspondingly less efficient suppressor of +1 frameshift mutations. These phenotypes indicate that translation and cell growth are highly sensitive to changes in EF-1alpha and EF-1beta activity.

scholarly journals Four new species of Trichoderma in the Harzianum clade from northern China

MycoKeys ◽  
2020 ◽  
Vol 73 ◽  
pp. 109-132
Author(s):  
Xin Gu ◽  
Rui Wang ◽  
Quan Sun ◽  
Bing Wu ◽  
Jing-Zu Sun
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Elongation Factor ◽  
Northern China ◽  
Gene Encoding ◽  
Translation Elongation ◽  
Nuclear Rna ◽  
Encoding Gene ◽  
Elongation Factor 1 ◽  
Nuclear Rna Polymerase

The Harzianum clade of Trichoderma comprises many species, which are associated with a wide variety of substrates. In this study, four new species of Trichoderma, namely T. lentinulae, T. vermifimicola, T. xixiacum, and T. zelobreve, were encountered from a fruiting body and compost of Lentinula, soil, and vermicompost. Their colony and mycelial morphology, including features of asexual states, were described. For each species, their DNA sequences were obtained from three loci, the internal transcribed spacer (ITS) regions of the ribosomal DNA, the gene encoding the second largest nuclear RNA polymerase subunit (RPB2), the translation elongation factor 1-α encoding gene (TEF1-α). The analysis combining sequences of the three gene regions distinguished four new species in the Harzianum clade of Trichoderma. Among them, T. lentinulae and T. xixiacum clustered with T. lixii, from which these new species differ in having shorter phialides and smaller conidia. Additionally, T. lentinulae differs from T. xixiacum in forming phialides with inequilateral to a strongly-curved apex, cultural characteristics, and slow growth on PDA. Trichoderma vermifimicola is closely related to T. simmonsii, but it differs from the latter by producing phialides in verticillate whorls and smaller conidia. Trichoderma zelobreve is the sister species of T. breve but is distinguished from T. breve by producing shorter and narrower phialides, smaller conidia, and by forming concentric zones on agar plates. This study updates our knowledge of species diversity of Trichoderma.

Morosphaeria muthupetensis sp. nov. (Morosphaeriaceae) from India: morphological characterization and multigene phylogenetic inference

2018 ◽  
Vol 61 (4) ◽  
pp. 395-405 ◽  
Author(s):  
Bandarupalli Devadatha ◽  
Vemuri Venkateswara Sarma ◽  
Rajesh Jeewon ◽  
E.B. Gareth Jones
Keyword(s):  
Tamil Nadu ◽  
Large Subunit ◽  
Elongation Factor ◽  
Small Subunit ◽  
Morphological Characterization ◽  
Internal Transcribed Spacers ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1 ◽  
Mangrove Wood

Abstract A novel species of Morosphaeria, Morosphaeria muthupetensis, saprobic on intertidal mangrove wood of Rhizophora mucronata from Muthupet mangroves on Kaveri River Delta, Tamil Nadu along the East coast of India, is described in this paper. Morosphaeria muthupetensis is characterized by large sub-globose to lenticular ascomata; long cylindrical to clavate, short pedicellate asci; small, fusiform to ellipsoidal ascospores with acute ends when compared to other Morosphaeria species. A multigene phylogeny obtained from a combined nuclear large subunit, small subunit, internal transcribed spacers rDNA and translation elongation factor 1-alpha sequence analysis revealed that M. muthupetensis is phylogenetically distinct and nested in between Morosphaeria velatispora and Morosphaeria ramunculicola and all these species constitute a strongly supported monophyletic clade sister to the genus Helicascus. Detailed descriptions and illustrations of M. muthupetensis are presented and compared with other Morosphaeria species.

scholarly journals Three new species of soil-inhabiting Trichoderma from southwest China

MycoKeys ◽  
2018 ◽  
Vol 44 ◽  
pp. 63-80 ◽  
Author(s):  
Min Qiao ◽  
Xing Du ◽  
Zhe Zhang ◽  
JianPing Xu ◽  
ZenFen Yu
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Elongation Factor ◽  
Gene Encoding ◽  
Translation Elongation ◽  
Closely Related Species ◽  
Encoding Gene ◽  
Three New Species ◽  
Elongation Factor 1 ◽  
Nuclear Rna Polymerase

Fungi in the genus Trichoderma are widely distributed in China, including in Yunnan province. In this study, we report three new soil-inhabiting species in Trichoderma, named as T.kunmingense, T.speciosum and T.zeloharzianum. Their colony and mycelial morphology, including features of asexual states, were described. For each species, their DNA sequences were obtained from three loci, the internal transcribed spacer (ITS) regions of the ribosomal DNA, the translation elongation factor 1-α encoding gene (tef1) and the gene encoding the second largest nuclear RNA polymerase subunit (rpb2). Our analyses indicated that the three new species showed consistent divergence amongst each other and from other known and closely related species. Amongst the three, T.speciosum and T.kunmingense belong to the Viride Clade. Specifically, T.speciosum is related to three species – T.hispanicum, T.samuelsii and T.junci and is characterised by tree-like conidiophores, generally paired branches, curved terminal branches, spindly to fusiform phialides and subglobose to globose conidia. In contrast, T.kunmingense morphologically resembles T.asperellum and T.yunnanense and is distinguished by its pyramidal conidiophores, ampulliform to tapered phialides, discrete branches and ovoidal, occasionally ellipsoid, smooth-walled conidia. The third new species, T.zeloharzianum, is a new member of the Harzianum Clade and is closely associated with T.harzianum, T.lixii and T.simmonsii but distinguished from them by having smaller, subglobose to globose, thin-walled conidia.

Phylogenetic classification and generic delineation of Hydeomyces desertipleosporoides gen. et sp. nov., (Phaeosphaeriaceae) from Jebel Akhdar Mountain in Oman

Phytotaxa ◽  
2019 ◽  
Vol 391 (1) ◽  
pp. 28 ◽  
Author(s):  
SAJEEWA S. N. MAHARACHCHIKUMBURA ◽  
HIRAN A. ARIYAWANSA ◽  
DHANUSHKA N. WANASINGHE ◽  
MONIKA C. DAYARATHNE ◽  
NADIYA A. AL-SAADY ◽  
...  
Keyword(s):  
Large Subunit ◽  
Elongation Factor ◽  
Small Subunit ◽  
Internal Transcribed Spacers ◽  
New Taxon ◽  
Small Subunit Rrna ◽  
Translation Elongation ◽  
The Family ◽  
Large Subunit Rrna ◽  
Elongation Factor 1

Specimens of a new pleosporalean taxon were obtained on the bark of Juniperus excels from the northern mountains of Oman; from the Jebel Akhdar (‘Green Hills’). Sequence analyses based on the regions of large subunit rRNA (LSU), small subunit rRNA (SSU), translation elongation factor 1-α (TEF) and internal transcribed spacers (ITS) were performed to resolve the phylogenetic relationships of the new taxon in Phaeosphaeriaceae. The data concluded that the taxon represents a novel genus of the family Phaeosphaeriaceae and the generic name Hydeomyces and the species name H. desertipleosporoides are introduced for the new taxon. An outline of the characters which differentiate the new genus from phylogenetically closely related genera Dematiopleospora and Dlhawksworthia is given and its morphology of asexual and sexual morphs is described.

scholarly journals A morphological and molecular survey of Neoconidiobolus reveals a new species and two new combinations

2021 ◽  
Author(s):  
Yong Nie ◽  
Zi-Min Wang ◽  
Xiao-Yong Liu ◽  
Bo Huang
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Elongation Factor ◽  
Small Subunit ◽  
New Combinations ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1 ◽  
A New Species

Abstract The genus Neoconidiobolus was recently established to accommodate all members of the Conidiobolus subgenus Conidiobolus. Based on mitochondrial small subunit (mtSSU), nuclear large subunit (nucLSU) of rDNA and translation elongation factor 1-alpha (TEF1), this study further resolved the genus Neoconidiobolus into three clades, with three new taxa being added. They are N. kunyushanensis B. Huang & Y. Nie, sp. nov., N. lamprauges (Drechsler) B. Huang & Y. Nie, comb. nov., and N. nanodes (Drechsler) B. Huang & Y. Nie, comb. nov. Meanwhile, a morphologial comparison among species in the three clades and a key to the species of the genus Neoconidiobolus are provided herein.

scholarly journals A taxonomic revision of the genus Conidiobolus (Ancylistaceae, Entomophthorales): four clades including three new genera

MycoKeys ◽  
2020 ◽  
Vol 66 ◽  
pp. 55-81 ◽  
Author(s):  
Yong Nie ◽  
De-Shui Yu ◽  
Cheng-Fang Wang ◽  
Xiao-Yong Liu ◽  
Bo Huang
Keyword(s):  
Ribosomal Dna ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Taxonomic Revision ◽  
Small Subunit ◽  
Nuclear Ribosomal Dna ◽  
New Genera ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

The genus Conidiobolus is an important group in entomophthoroid fungi and is considered to be polyphyletic in recent molecular phylogenies. To re-evaluate and delimit this genus, multi-locus phylogenetic analyses were performed using the large and small subunits of nuclear ribosomal DNA (nucLSU and nucSSU), the small subunit of the mitochondrial ribosomal DNA (mtSSU) and the translation elongation factor 1-alpha (EF-1α). The results indicated that the Conidiobolus is not monophyletic, being grouped into a paraphyletic grade with four clades. Consequently, the well-known Conidiobolus is revised and three new genera Capillidium, Microconidiobolus and Neoconidiobolus are proposed along with one new record and 22 new combinations. In addition, the genus Basidiobolus is found to be basal to the other entomophthoroid taxa and the genus Batkoa locates in the Entomophthoraceae clade.

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